• Journal of Aquaculture
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• 제18권3호
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• pp.189-195
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• 2005

By testing the separation performance for a fine settleable solid removal system in an aquaculture system using polystyrene particles as an experimental substitute, the optimal geometric dimensions for a Low-Pressure Hydrocyclone (LPH) were obtained. The design approach far the LPH took into consideration two inflow diameters (Di: 30, 50 mm), three overflow diameters (Do: 60, 70, 100 mm) and four cylinder lengths (Lc: 250, 345, 442, 575 mm), while the cylinder diameter (Dc) at 335 mm, the underflow diameter (Du) at 50 mm and the cone angle (${\theta}$) at $68^{\circ}$ were kept constant. The separation performances of 19 different dimension combinations of LPH were tested, ranging from 300 to 1200 ml/sec of inflow rate using substitute polystyrene particles (0.4-0.7 mm dia., ${\rho}_s=1.05g/cm^3$). These polystyrene particles exhibit a similar density and settling velocity to the fine fecal debris of the common carp. The total separation efficiency for the inflow rate ranged from a high of 97% to a low of 20%. Experimental results obtained by ANCOVA and the Tukey test (${\alpha}=0.05$) showed that the separation performances of the LPH were significantly affected (P<0.05) by the fi, Di, Do and Lc. The maximum separation performance was detected at a dimension combination of 30 mm of inflow diameter (Di), 60 mm of overflow diameter (Do), 442 and 575 mm of cylinder length (Lc). The dimension proportions were 0.09, 1.32-1.72, 0.18 and 0.15 for Di/Dc, Lc/Dc, Do/Dc and Du/Dc respectively.

• Bulletin of the Korean Chemical Society
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• 제31권8호
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• pp.2322-2328
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• 2010

A multiple solid-phase extraction (SPE) method was used with liquid chromatography, coupled with mass spectrometry (LC/MS), for the analysis of heterocyclic amines (HCAs) in human urine. Separation efficiencies based on the pH of the mobile phase and the types of columns were compared. An amide column showed better baseline separation and narrower HCA peak widths at pH 5.0 for the mobile phase than a $C_8$ column. Each SPE step, HLB, MCX, and HybridSPE, was optimized by controlling the pH conditions. The combined method with the three SPEs effectively removed interfering species that cause ion-suppression during HCA detection. Validation of the method, performed with SIM and SRM detection, showed correlation coefficients above 0.991 in the range 0.3 - 16.7 ng/mL. Recovery rates were 45.4 - 97.3% on the $C_8$ column and 71.8 - 101.4% on the amide column, and method detection limits were 0.11 - 0.65 ng/mL on the $C_8$ column and 0.12 - 0.48 ng/mL on the amide column. This method using multiple SPEs offers significant benefits for high-throughput determination of HCAs in urine.

• Journal of Korean Society of Water and Wastewater
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• 제24권3호
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• pp.267-276
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• 2010

Many plants have been improved to adapt the target of the biological treatment processes changed from organics to nutrients since the water quality criteria of effluent was reinforced and included T-N and T-P for the municipal wastewater treatment plant. To meet the criteria of T-N and T-P, the conventional biological reactor such as aeration tank in activated sludge system is changed to the BNR (biological nutrient removal) processes, which are typically divided into three units as anaerobic, anoxic and oxic tank. Therefore, the solid separation process should be redesigned to fit the BNR processes in case of the application of the DAF (dissolved air flotation) process as an alternatives because the solid-liquid separation characteristics of microbial flocs produced in the BNR processes are also different from that of activated sludge system as well. The results of this study revealed that the microbial floc of the anaerobic tank was the hardest to be separated among the three steps of the unit tanks for the BNR processes. On the contrary, the oxic tank was best for the removal efficiency of nutrients as well as suspended solid. In addition, the removal efficiency of nutrients was much improved under the chemical coagulation treatment though coagulation was not indispensable with a respect to the solid separation. On the other hand, in spited that the separation time for the microbial floc from the BNR processes were similar to the typical particles like clay flocs, over $2.32{\times}10^3$ ppm of air volume concentration was required to keep back the break-up of the bubble-floc agglomerates.

• Advances in nano research
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• 제10권3호
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• pp.295-312
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• 2021

A new ionic liquid functionalized magnetic silica nanoparticle was synthesized and characterized and tested as an adsorbent. The adsorbent was used for magnetic solid phase extraction on ICP-MS method. Simultaneous determination of precious metal Au has been addressed. The method is simple and fast and has been applied to standard water and surface water analysis. A new method for separation/analysis of trace precious metal Au by Magnetron Solid Phase Extraction (MSPE) combined with ICP-MS. The element to be tested is rapidly adsorbed on CoFe2O4@SiO2@[BMIM]PF6 composite nano-adsorbent and eluted with thiourea. The method has a preconcentration factor of 9.5-fold. This method has been successfully applied to the determination of gold in actual water samples. Hydrophobic Ionic Liquids (ILs) 1-butyl-3-methylimidazole hexafluorophosphate ([BMIM]PF6) coated CoFe2O4@SiO2 nanoparticles with core-shell structure to prepare magnetic solid phase extraction agent (CoFe2O4@SiO2@ILs) and establish a new method of MSPE coupled with inductively coupled plasma mass spectrometry for separation/analysis of trace gold. The results showed that trace gold was adsorbed rapidly by CoFe2O4@SiO2@[BMIM]PF6 and eluanted by thiourea. Under the optimal conditions, preconcentration factor of the proposed method was 9.5-fold. The linear range, detection limit, correlation coefficient (R) and relative standard deviation (RSD) were found to be 0.01~1000.00 ng·mL-1, 0.001 ng·mL-1, 0.9990 and 3.4% (n = 11, c = 4.5 ng·mL-1). The CoFe2O4@SiO2 nanoparticles could be used repeatedly for 8 times. This proposed method has been successfully applied to the determination of trace gold in water samples.

• Journal of the Korean Society of Marine Environment & Safety
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• 제11권2호
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• pp.109-115
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• 2005

Hydrocyclone has been widely used for the solid-liquid separation in many industrial sites because of its comparatively preferable applications that can be applied to wide-range particle sizes. If seawater with impurities flows through pumps or heat exchanger, it might cause an decrease in the efficiency of cooling system In this paper, we have suggested some methods of separating impurities from seawater in the cooling system by using a Hydrocyclone. The effects of design factors as solid concentration, cyclone inlet pressure, flow rate and diameter of underflow on the separating performance of the Hydrocyclone were investigated The results from this study are summarized as follows: 1) In proportion to the decrease of solid concentration, the efficiency of solid-liquid separation is improved. 2) According as the cyclone inlet pressure increases the efficiency of separation is improved. Conclusively, this research suggested that the Hydrocyclone will be used as a pre-treatment system of cooling water in machines, and eventually prevent unexpected accidents in engine systems.

• Proceedings of KOSOMES biannual meeting
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• 해양환경안전학회 2004년도 추계학술발표회
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• pp.109-115
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• 2004

Hydrocyclone has been widely used for the solid-liquid separation in many industrial sites because of its comparatively preferable applications that can be applied to wide-range particle sizes. If seawater with impurities flows through pumps or heat exchanger, it might cause an decrease in efficiency of cooling system. In this paper, we have suggested some methods of separating impurities from seawater in the cooling system by using a Hydrocyclone. The effects of design factors as solid concentration, cyclone inlet pressure, flow rate and diameter of underflow on the separating performance of the Hydrocyclone were investigated The results from this study are summarized as follows: 1) In proportion to the decrease of solid concentration, the efficiency of solid-liquid separation is improved 2) According as the cyclone inlet pressure increases the efficiency of separation is improved Conclusively, this research suggested that the Hydrocyclone will be used as a pre-treatment system of cooling water in machines, and eventually prevent unexpected accidents in engine systems.

• Journal of Korean Society of Water and Wastewater
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• 제22권5호
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• pp.531-540
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• 2008

The main advantage of dissolved air flotation (DAF) in water treatment process is the small dimension compared with conventional gravity sedimentation and it can be basically reduced by the separation zone performed with the short solid-liquid separation time. Fine bubbles make such a short time possible to carry out solid from liquid separation as a collector on the course of water treatment. Therefore, the dimension of separation zone in DAF process is practically determined by the rise velocity of the bubble-floc agglomerates, which is a floc attached with several bubbles. To improve flotation velocity and particle removal efficiency in DAF process, many researchers have tried to attach bubbles as much as possible to flocs. Therefore, the maximum number of attached bubble on a floc and the rise velocity of bubble-floc agglomerates considered as the most important factor to design the separation zone of flotation tank in DAF process was simulated based on the population balance theory. According to the simulation results of this study, the size and volume concentration of bubble influenced on the possible number of attached bubble on a floc. The agglomerates attached with smaller bubble was more sensitive to hydraulic loading rate in the separation zone of DAF process. For the design of a high rate DAF process applied over surface loading 40 m/hr. it is required a precise further study on the variation of bubble property and behavior including in terms of bubble size distribution.

• Journal of Animal Environmental Science
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• 제17권1호
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• pp.33-38
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• 2011

Economic development involves increase in life expectancy as well as human health care. Consequently, demand for fish meal and fish oil is rapidly growing. In particular, Krill (Eupausia superba) oil product is in high demand due to its rich unsaturated-fatty acid, and thus stable supplies are necessary in the krill oil market. It is required for captured krills to be immediately frozen and stored during ship transport, since proteins of the krill are quickly denatured in natural temperature condition. However, the transportation cost has been sharply increased, which encourages researchers to involve in studies for development of efficient oil extraction process. In this study, a solid/liquid separation technique on boat for the krill oil was developed through triple separation tests using only a separator or using either brush or crusher prior to the separator. The separation tests revealed that the efficiency were 46.2, 60.2 and 60.4 % by the separator, combination with brush, and combination with crusher, respectively. In addition, it was found that byproduct, extracted cake, derived from the separation process could be used as a feed stuff. These results suggest that smashing using the brush or crusher prior to the separator is more efficient than using only the separator.

• Journal of Animal Environmental Science
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• 제19권2호
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• pp.177-182
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• 2013

We studied the possibility to produce solid fuel using cattle manure and to apply TAO (Thermophilic Aerobic Oxidation) process of solid-liquid separation fraction. The physiochemical compositions of cattle manure solid fuel chip were analyzed as water 0.12%, low calorific value 3,510 kcal/kg, ashes 11.9%, chlorine 0.82%, sulfur dust 0.5%, mercury non-detection, cadmium 1.0 mg/kg, lead 2 mg/kg, arsenic non-detection. In treating cattle manure with TAO reactor the internal temperature of the reactor was increasing higher and $50^{\circ}C$ and over was maintained after 20 hours on. The physiochemical compositions of liquids increased from pH 7.3 to pH 9.18 and EC decreased from 4.6 to 3.48 mS/cm in treating process of cattle manure with TAO reactor. COD and SCOD decreased from 16,800 to 10,400 mg/L, from 4,600 to 2,040 mg/L respectively, which showed about 38% and 56% of remove efficiency respectively.

• FOCUS: LIFE SCIENCE
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• 제1호
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• pp.3.1-3.7
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• 2024

The article discusses the critical role of chromatography in the analysis and purification of proteins in biopharmaceuticals, emphasizing the importance of comprehensive characterization for ensuring their safety and efficacy. It highlights the use of Avantor® ACE® HPLC columns for the separation and purification of proteins, focusing on the analysis of intact proteins using reversed-phase liquid chromatography (RPLC) with fully porous particles. This article also details the application of different mobile phase additives, such as TFA and formic acid, and emphasizes the advantages of using type B ultra-pure silica-based columns for efficiency and peak shape in biomolecule analysis. Additionally, it addresses the challenges of analyzing intact proteins due to slow molecular diffusion and introduces the concept of solid-core (or superficially porous) particles, emphasizing their benefits over traditional porous particles for the analysis of therapeutic proteins. Furthermore, it discusses the development of Avantor® ACE® UltraCore BIO columns, specifically designed for the high-efficiency separation of large biomolecules, such as proteins, and demonstrates their effectiveness in achieving high-resolution separations, even for higher molecular weight proteins like monoclonal antibodies (mAbs). In addition, it underscores the complexity of analyzing and characterizing intact protein biopharmaceuticals, requiring a range of analytical techniques and the use of wide-pore stationary phases, operated at elevated temperatures and with relatively shallow gradients. It highlights the comprehensive range of options offered by Avantor® ACE® wide pore columns, including both fully porous and solid-core particles, bonded with a variety of complementary stationary phase chemistries to optimize selectivity during method development. The use of ultrapure and highly inert base silica is emphasized for enabling the use of lower concentrations of mobile phase modifiers without compromising analyte peak shape, particularly beneficial for LC-MS applications. Then the article concludes by emphasizing the significance of reversed-phase liquid chromatography and its compatibility with mass spectrometry as a valuable tool for the separation and analysis of intact proteins and their closely related variants in biopharmaceuticals.