• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.3
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• pp.147-151
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• 2003

Normal seed tubers and plug seedlings raised from stem cutting and mini-tubers ($10\pm3$ g) were planted or transplanted on August 20,2001 in bare soil or under transparent and black polyethylene film mulches to evaluate the effects of mulch material and seedling type on the growth and tuber yield of fall-grown potato (Solanum tuberosum L. cv. Dejima) in Jeju region. Total tuber number per plant was 4.6 regardless of mulch treatment, and direct planting seed potatoes resulted in the greatest tubers (5.14)/plant and followed by transplanting seedlings raised from cutting and mini-tubers (4.15 and 5.15 tubers/plant). Transparent and black polyethylene film mulches increased total tuber yield by 16 and 8%, respectively, and average tuber weight by 14 and 12%, respectively, compared with no mulch (21.16 t/ha and 72.2 g/tuber). Transplanting plug seedlings raised from cutting resulted in the greatest tuber yield, followed by transplanting plug seedlings raised from mini-tubers and direct planting seed potatoes.

• Korean Journal of Plant Tissue Culture
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• v.24 no.1
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• pp.49-52
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• 1997

The effects of several additives on plant regeneration were investigated from leaf disc culture of S. tuberosum cv. Atlantic which is known as poor in regeneration ability. The presence of 2 g/L casein hydrolysate significantly enhanced shoot regeneration. Addition of 10~20 $\mu$M $AgNO_3$, not only increased the frequency of shoot regeneration but also maintained the leaf disc green presumably by the inhibitory action of ethylene accumulation in vitro. Decrease of sucrose levels to below 3% significantly increased the degree of regeneration. The addition of CuSO$_4$had no effect on shoot regeneration.

• Plant Biotechnology Reports
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• v.5 no.3
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• pp.265-271
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• 2011

The cultivated potato as well as its tuber-bearing relatives are considered model plants for cell and tissue culture, and therefore for exploiting the genetic variation induced by in vitro culture. The association between molecular stability and tissue culture in different genetic backgrounds and ploidy levels has already been explored. However, it still remains to be ascertained whether somaclonal variation differs between callus-derived chromosome-doubled and undoubled regenerants. Our research aimed at investigating, through amplified fragment length polymorphism (AFLP) markers, the genetic changes in marker-banding patterns of diploid and tetraploid regenerants obtained from one clone each of Solanum bulbocastanum Dunal and S. cardiophyllum Lindl (both 2n = 2x = 24) and tetraploids from cultivated S. tuberosum L. (2n = 4x = 48). Pairwise comparisons between the banding patterns of regenerants and parents allowed detecting considerable changes associated to in vitro culture both at diploid and tetraploid level. The percentages of polymorphic bands between diploid and tetraploid regenerants were, respectively, 57 and 69% in S. bulbocastanum and 58 and 63% in S. cardiophyllum. On average, the frequencies of lost parental fragments in regenerants were significantly higher than novel bands both in S. bulbocastanum (48 vs. 22%) and S. tuberosum (36 vs. 18%) regenerants. By contrast, in S. cardiophyllum, a similar incidence of the two events was detected (32 vs. 29%). Our results revealed that structural changes after tissue culture process strongly affected the genome of the species studied, but diploid and tetraploids regenerated plants responded equally.

• Korean Journal of Weed Science
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• v.17 no.4
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• pp.390-399
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• 1997

This experiment was conducted to introduce PAT (phosphinothricin acetyltransferase, non-selective herbicide bialaphos resistant gene) gene into potato (Solanum tuberosum. cv. Desiree). Optimal shoot regeneration from leaf discs and stem segments was obtained in MS medium supplemented with 0.1 mg/L IBA and 0.5 mg/L BA, and the frequency of shoot regeneration was 54% in left discs and 46% in stem segments. In this condition, leaf discs and stem segments of potato were co-cultivated with A. tumefaciens MP90 which contained binary vector with GUS: :NPTII gene and PAT gene. Transgenic shoots were regenerated from leaf and stem-derived calli on selection medium with 100mg/L kanamycin. The 100${\mu}M$ acetosyringone treatment during the co-cultivation highly enhanced(4 times than the control) the shoot regeneration on selection medium. When the putative transgenic plants were transferred to medium with 10mg/L basta, all of them were survived. After PCR. GUS test, and Southern blot analysis of the survived plant, we confirmed that the gene was stably integrated into the potato genome and expressed. After the transgenic plants were transplanted in soil, and the transgenic plants were sprayed with the herbicide basta (300ml/10a), the transgenic plants remained green but control plants were died.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.5
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• pp.905-909
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• 2002

This study was peformed to determine the anticarcinogenic activity of the Solanum tuberosum Peel (SP) on several microorganisms and human cancer cell lines. Among the various solvent fractions of SP, the ethylether Partition layer (SPMEE) showed the strongest antimicrobial activity, ethylacetate partition layer (SPMEA) and butanol partition layer (SPMB) resulted in good antimicrobial activity. We also determined the effect of SP extract and fractions on cytotoxicity, and chemopreventive effect on human cancer cells. The experiment was conducted to determine cytotoxicity of SP partition layers on HepG2, HeLa and MCF-7 cells by MTT assay. Among the various partition layers of SP, SPMEE and SPW were showed the strongest cytotoxic effects on all cancer cell lines. The quinone reductase induced activities of HepG2 cell, the butanol partition layer (SPMB) at a does of 40 $\mu\textrm{g}$/mL was 8.49 times more effective compared to the control value of 1.0. This value was significantly higher than that of previous results using the other materials. Therefore, based on these studies, SP may be developed into a potentially useful antimicrobial and anticarcinogenic agents.

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.551-554
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• 1998

Bacterial specs isolated from common scab lesion on potato (Solanum tuberosum L. c.. Dejima) tuber was identified as Streptomyces turgidiscabies. This organism had flexuous spore chains and grey spore mass color, produced melanin pigment on ISP 7, but did not produce on ISP 6. S. turgidiscabies grew on agar media at pH 4.5, used L-arabinose, D-fructose, D-glucose, D-mannitol, raffinose, rhamnose, sucrose, D-xylose and meso-inositol as carbon sources, and was susceptible to 7% NaCl, thallium acetate (10 $\mu\textrm{g}$/ml, 100$\mu\textrm{g}$/ml), crystal violet (0.5 $\mu\textrm{g}$/ml), phenol (0.1%, wt/vol), oleandomycin (100 $\mu\textrm{g}$/ml), and streptomycin (20 $\mu\textrm{g}$/ml).

• Korean Journal Plant Pathology
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• v.14 no.6
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• pp.689-692
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• 1998

Bacterial species isolated from common scab lesion on potato (Solanum tuberosum L. cv. Dejima) was identified as Streptomyces acidiscabies. This organism had flexuous spore chains and white spore mass color, produced melanin pigment on tyrosine agar medium but did not produce on peptone agar medium. S. acidiscabies grew on agar medium at pH 4.0, used L-arabinose, D-fructose, D-glucose, D-mannitol, rhamnose, sucrose, D-xylose and meso-inositol except reffinose as carbon sources. It was also susceptible to thallium acetate (10 $\mu\textrm{g}$/ml, 100$\mu\textrm{g}$/ml), phenol (0.1%, wt/vol), streptomycin (20 $\mu\textrm{g}$/ml), and was resistant to 7% NaCl, crystal violet (0.5 $\mu\textrm{g}$/ml), penicillin (10 IU/ml) and oleandomycin (25 $\mu\textrm{g}$/ml, 100 $\mu\textrm{g}$/ml).

• Journal of Bio-Environment Control
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• v.14 no.4
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• pp.298-301
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• 2005

Potato (Solamum tuberosum 'Dejima') plantlets were investigated on culture type and initial quantity of inoculation in bioreactor and survival rate by hydroponics for mass production. rode stems (1 to 1.5cm in length) of potato plantlets multiplied in vitro were grown for 3 weeks in liquid Murashige and Skoog (MS) medium with sucrose $30 g\; L^{-1}$. When plantlets (80-node inoculation) were raised in 10L balloon type bubble (BB) bioreactor, the healthiest growth of plantlets was obtained from explants cultured in ebb & flow culture with medium supplied periodically 12 times per day. The suitable inoculation quantity of 20L BB bioreactor was 120 pieces of stem segments (mean 2.2g fresh weight) in ebb & flow culture. Number of nodal shoot was eight on the average. In controlled culture room, survival rate of plantlets at 7 days after stem cutting was above $70\%$ when they were acclimatized by hydroponics grown in deep flow and solid medium culture. The highest survival rate of the stem cutting plantlets was in nutrient solution adjusted to EC $1.4dS{\cdot}m^{-1}$. Stem cutting plantlets through one culture could be obtained $670\~900$, when plantlets were grown in ebb & flow culture during 3 weeks using a 20L bioreactor with initial 120 pieces of nodal segments. 11 is possible In do mass production of seedlings cultured in bioreactor and hydroponics.

• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.311-316
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• 2016

The efficacy of plant breeding has been enhanced by application of molecular markers in population screening and selection. Pepper (Capsicum annuum L.) is a major staple crop that is economically important with worldwide distribution. It is valued for its spicy taste and medicinal effect. The aim of this study was to discover single nucleotide polymorphisms (SNPs), microsatellite markers information, and percentage sharing through orthologous analysis of pepper-specific pungency-related genes. Here, we report the results of transcriptome analysis and microsatellite markers for four pepper varieties that possess a pungency-related gene. Orthologous analyses was performed to identify species-specific pungency-related genes in pepper, Arabidopsis thaliana L., potato (Solanum tuberosum L.), and tomato (Solanum lycopersicum L.). Advancements in next-generation sequencing technologies enabled us to quickly and cost-effectively assemble and characterize genes to select molecular markers in various organisms, including pepper. We identified a total of 9762, 7302, 8596, and 6886 SNPs for the four pepper cultivars Blackcluster, Mandarine, Saengryeg 211, and Saengryeg 213, respectively. We used 454 GS-FLX pyrosequencing to identify microsatellite markers and tri-nucleotide repeats (54.4%), the most common repeats, followed by di-, hexa-, tetra-, and penta-nucleotide repeats. A total of 5156 (15.9%) pepper-specific pungency-related genes were discovered as a result of orthologous analysis.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2002.04a
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• pp.93-96
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• 2002

감자는 오랜 재배 역사를 가진 세계4대 식량작물로 영양가가 풍부하며 생산량이 어느 작물보다 많기 때문에 전략식량으로 많이 이용되고 있다. 최근 가공산업의 발달로 감자의 수요가 급격히 증가함에 따라 감자의 대량 생산에 관련된 많은 연구들이 수행되어 왔으며, 그 중 미세증식 기법은 단기간에 대량 생산할 수 있다는 점에서 각광을 받고 있다. (중략)