• Horticultural Science & Technology
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• v.30 no.4
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• pp.426-431
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• 2012

This study was conducted to establish an efficient screening method for resistant tomato to Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (FOL). The resistance degrees of the six commercial cultivars of tomato to the pathogen were evaluated by dipping roots of the seedlings in spore suspension of five FOL isolates. On the basis of the results, two cultivars (Dotaerangmaster, resistant cultivar to FOL race 1; Supersunload, resistant cultivar to FOL race 2) and two isolates (KACC40043, FOL race 2; TF104, FOL race 3) were selected for system establishment. The disease development of the FOL isolates on the cultivars according to several conditions including root wounding, incubation temperature, inoculum concentration and dipping period of roots in spore suspension was investigated. The resistance of each cultivar to the disease was a race-specific response and hardly affected by the tested conditions except for incubation temperature of $20^{\circ}C$. The optimum temperature for disease development caused by FOL was 25 to $30^{\circ}C$. On the basis of the results, we suggest that an efficient screening method for resistant tomato cultivars to Fusarium wilt is to dip the non-cut roots of tomato seedlings at two-leaf stage in spore suspension of $1{\times}10^7\;conidia{\cdot}mL^{-1}$ for 0.5 hours and transplant the seedling to plastic pot with horticulture nursery media, and then to cultivate the plants in a growth room at $25^{\circ}C$ for 3 weeks with 12 hours light a day.

• Journal of Bio-Environment Control
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• v.27 no.4
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• pp.302-311
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• 2018

Using cylindrical paper pot nursery method, three kinds of commercial tomatoes 'Dafnis', 'DOTAERANG DIA' and 'Maescala' were grafted onto a commercial rootstock 'B blocking'. From 10 to 40 days after graft-take, growth traits of seedlings were investigated by 0.5, 1.0 and 2.0S treatments of standard nutrient solution(S) for seedling growth, and top to root ratio(TRR), compactness(CP) and Dickson Quality Index(DQI) were calculated. Two weeks after transplanting of the seedlings under three different night temperature targeting to 10, 15, and $25^{\circ}C$, which were not precisely controlled, the relative growth rate (RGR) was investigated. The quantitative growth traits of grafted seedlings increased with increasing fertilizer concentration, and various range of seedling size could be produced. Compactness and DQI were significantly regressed (Adj $R^2=0.9480$). Short-term RGR after transplanting was higher at 1.0S treatment of standard nutrient solution at the seedling age of 30 days and 40 days after graft-take(DAGT). DQI and RGR were significantly regressed linearly at respective fertigation strength. Specially the diminishing slope of RGR was lower at 1.0S fertigation strength with the increase of DQI than others. The results indicate that DQI could be applied as a quality index of grafted tomato seedlings and the relation analysis between DQI and short-term RGR also could be used to determine the good quality seedlings of grafted tomato grown in cylindrical paper pot.

• Journal of the Korea Organic Resources Recycling Association
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• v.27 no.1
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• pp.39-48
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• 2019

Objective of this study was to evaluate an optimum biochar application rate and estimate the carbon sequestration based on the soil chemical properties and growth responses for biochar application during tomatoes cultivation. The treatments consisted of control as recommended application rates of fertilizers, 0.01%, 0.03%, 0.05%, and 0.07% of biochar application(w/w, biochar:soil). For effects of soil chemical properties, the $NO_3-N$contents in the soil were peaked at 9 days after transplanting. But there was not significant difference(p>0.05) among the treatments during cultivation periods. However, $NH_4-N$ contents in the biochar treatment were lower than the control until 14 days of transplanting. $P_2O_5$ contents in the biochar treatments were lower than that of the control until 19 days after transplanting except 0.01% of biochar application plot. $K_2O$ contents in soils treated with 0.01% and 0.03% of biochar were higher until 6 days after transplanting than that in the control. For N use efficiency of biochar application, it was observed that the 0.05% biochar application plot was highest among the treatments. The highest carbon sequestration was estimated at $2.83mg\;kg^{-1}$ for 0.03% of biochar application. However, it is considered that the optimum biochar application rate was 0.05% for tomato cultivation, considering the growth characteristics and yield components.

• Journal of Bio-Environment Control
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• v.28 no.1
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• pp.38-45
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• 2019

Recycling of drained nutrient solution in hydroponic cultivation of horticultural crops is important in the conservation of the water resources, reduction of production costs and prevention of environmental contamination. Objective of this research was to obtain the fundamental data for the development of a recirculation system of hydroponic solution in semi-forcing cultivation of 'Bonus' tomato. To achieve the objective, tomato plants were cultivated for 110 days and the contents of inorganic elements in plant, supplied and drained nutrient solution were analyzed when crop growth were in the flowering stage of 2nd to 8th fruiting nodes. The T-N content of the plants based on above-ground tissue were 4.1% at the flowering stage of 2nd fruiting nodes (just after transplanting), and gradually get lowered to 3.9% at the flowering stage of 8th fruiting nodes. The tissue P contents were also high in very early stage of growth and development and were maintained to similar contents in the flowering stage of 3rd to 7th fruiting nodes, but were lowed in 8th node stages. The tissue Ca, Mg and Na contents in early growth stages were lower than late growth stages and the contents showed tendencies to rise as plants grew. The concentration differences of supplied nutrient solution and drained solution in $NO_3-N$, P, K, Ca, and Mg were not significant until 5 weeks after transplanting, but the concentration of those elements in drained solution rose gradually and maintained higher than those in supplied solution. The concentrations of B, Fe, and Na in drained solution were slightly higher in the early stages of growth and development and were significantly higher in the mid to late stages of growth than those in supplied solution. The above results would be used as a fundamental data for the correction in the inorganic element concentrations of drained solution for semi-forcing hydroponic cultivation of tomato.

• Journal of Plant Biotechnology
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• v.46 no.3
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• pp.165-171
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• 2019

Marker-assisted backcrossing (MABC) is useful for selecting an offspring with a highly recovered genetic background for a recurrent parent at early generation to various crops. Moreover, marker-assisted backcrossing (MABC) along with marker-assisted selection (MAS) contributes immensely to overcome the main limitation of the conventional breeding and it accelerates recurrent parent genome (RPG) recovery. In this study, we were employed to incorporate rin gene(s) from the donor parent T13-1084, into the genetic background of HK13-1151, a popular high-yielding tomato elite inbred line that is a pink color fruit, in order to develop a rin HK13-1084 improved line. The recurrent parent genome recovery was analyzed in early generations of backcrossing using SNP markers obtained from genotyping-by-sequencing analysis. From the $BC_1F_1$ and $BC_2F_1$ plants, 3,086 and 4868 polymorphic SNP markers were obtained via GBS analysis, respectively. These markers were present in all twelve chromosomes. The background analysis revealed that the extent of RPG recovery ranged from 56.7% to 84.5% and from 87.8% to 97.8% in $BC_1F_1$ and $BC_2F_1$ generations, respectively. In this study, No 5-1 with 97.8% RPG recovery rate among $BC_2F_1$ plants was similar to HK13-1151 strain in the fruit shape. Therefore, the selected plants were fixed in $BC_2F_2$ generation through selfing. MAS allowed identification of the plants that are more similar to the recurrent parent for the loci evaluated in the backcross generations. MABC can greatly reduce breeding time as compared to the conventional backcross breeding. For instance, MABC approach greatly shortened breeding time in tomato.

• Journal of Bio-Environment Control
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• v.31 no.3
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• pp.194-203
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• 2022

Cultivation of tomatoes in Korea grown in soil covers 89% of the total area for tomato cultivation. Tomatoes grown in soil often encounter various environment stresses including not only salt stress and soil-borne diseases but also cold stress in the winter season. This study was conducted to comparatively analyze the performance of rootstocks with cold stress by measuring the growth, yield, and photosynthetic efficiency in tomatoes grown in soil. The rootstocks were used 'Powerguard', 'IT173773', and '20LM' for the domestic rootstock cultivars and 'B-blocking' for a control cultivar. The tomato cultivar 'Red250' was used as the scion and the non-grafted tomatoes. Stem diameter, flowering position, leaf length, and leaf width were investigated for the growth parameters. The stem diameter of the non-grafted tomatoes decreased by 15% compared to the grafted tomatoes at 80 days after transplanting when exposed to low temperatures of 9-14℃ for 14 days. The leaf length and width of the non-grafted tomatoes were the lowest with 42.4 cm and 41.8 cm at 80 days after transplanting. The total yield per plant was the highest in tomato plants grafted on 'Powerguard' with 1,615 g and lowest in non-grafted tomatoes with 1,299 g. As the result of measuring the chlorophyll fluorescence parameters, PI_ABS and DI₀/RC, which mean the performance index and dissipated energy flux, 'Powerguard' was the highest with 3.73 in PI_ABS and the lowest with 0.34 in DI₀/RC, whereas non-grafted tomatoes was the lowest with 2.62 in PI_ABS and the highest with 0.41 in DI₀/RC at 80 days after transplanting. The stem diameter has positive correlation with PI_ABS, while it has negative correlation with DI₀/RC. The results indicate that can be analyzed by chlorophyll fluorescence parameters can be used for analyzing the differences in the growth of tomato plants grafted on different rootstocks when exposed to cold stress.