• 생물환경조절학회지
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• 제19권1호
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• pp.19-24
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• 2010

토마토 펄라이트 자루재배에서 기존에 알려진 배액 전극제어법 적용시기를 가능한 앞당기고, 배양액을 절감하기 위해 수행되었다. 실험처리는 정식후 15일차(T-15), 19일차(T-19), 22일차(T-22)에 배액전극제어법을 적용하는 3개 처리와 타이머법 1개 처리를 실시하였다. 기존의 정식후 1개월 이후에 배액전극제어법 적용하는 관행보다 2주 앞당긴 15일차 처리부터 작물이 배액전극제어법에 반응하는 것으로 나타났다. 생장 및 수확량은 배액전극법에서 높았다. 용수이용효율과 비료이용효율은 T-15 처리에서 가장 경제적이었고, 타이머법 처리는 경제성이 낮았다. 따라서 배액전극제어법 처리시기를 빨리 적용할수록 경제성이 높음을 알 수 있었다.

• The Plant Pathology Journal
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• 제16권2호
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• pp.110-117
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• 2000

Potato virus X (PVX-KO) showing mild mosaic and stunting symptoms on potato (Solanum tuberosum) in Kangwon area has been isolated and characterized. EM observation of the purified virus particles showed flexuous rod shape of about 520 nm in length. The coat protein (CP) of the virus had a molecular weight of 31 kDa in SDS-PAGE analysis, and the viral RNA was approximately 6.4 kb in size in denatured agarose gel electro-phoresis. In gel-immunodiffusion tests, it reacted strongly with an antiserum to common PVX from BIOREABAAG (USA). A rabbit antiserum was produced using purified virus and used for routine PVX detection by ELISA. Cultivated potatoes in Kangwon and other areas were frequently infected with PVX-KO. Both Datura stramonium and Nicotiana tabaccum cultivars developed necrotic local lesions 5 days after inoculation, and systemic mosaic symptoms with vein clearing 2 weeks after inoculation. All the features agree with the description of other PVX strains. To confirm and determine PVX strains, reverse transcription-polymerase chain reaction experiment was conducted using specific primers for viral CP. Amplified DNA fragments were cloned and sequenced. Results showed nucleotide sequence homologies of about 88 to 99% to other PVX strains. Based on CP amino acid sequence deduced from nucleotide sequences and host range studies PVX-KO is considered a member of the type X subgroup of PVX.

• 식물병연구
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• 제22권1호
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• pp.55-58
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• 2016

고추포장 주변의 모자이크 및 기형의 병징을 보이는 토끼풀에서 CMV를 분리하여 CMV-Tr1으로 명명하였다. 토끼풀로부터 RT-PCR, PCR-restriction fragment length polymorphism 및 외피단백질 유전자의 염기서열 분석을 통해 CMV를 동정하였다. 기주식물 반응결과 N. benthamiana를 포함한 5종의 담배와 오이, 꽈리 및 토마토의 상엽에서 모자이크 병징을 나타내었고, 명아주와 동부에서는 접종엽에 국부괴사 병반을 나타냈다. CMV-Tr1의 외피단백질을 CMV-As와 비교한 결과 99.5%의 상동성을 보였으며, 계통분석 결과 CMV subgroup IB에 속하는 CMV의 한 계통으로 확인되었다.

• 식물병연구
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• 제20권2호
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• pp.107-111
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• 2014

나선선충 접종 밀도가 토마토의 생육에 미치는 영향을 조사하고자, 시판 토마토 6품종을 직경 10-cm 토화분에 심고, 나선선충을 0, 0.02, 0.2, 2마리/g 토양 수준으로 접종하여 60일간 온실에서 재배하였다. 나선선충은 식물체의 무게나 뿌리의 무게에는 영향을 미치지 않았으나 키에는 영향을 주었는데(P < 0.05), 토양 g 당 나선선충 2마리 접종 시 포세이돈 품종은 무처리에 비하여 키가 24% 위축되었다. 나선선충을 접종하였을 때(2마리/g 토양), 가장 나선선충의 선충의 증식이 많았던 토마토 품종은 호용으로 최종 선충 밀도는 7.0마리/g 토양 이었고, 가장 나선선충의 선충의 증식이 적었던 토마토 품종은 미니흑수품종으로 최종 선충 밀도는 2.2마리/g 토양 이었다.

• The Plant Pathology Journal
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• 제36권4호
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• pp.346-354
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• 2020

Pectobacterium, which causes soft rot disease, is divided into 18 species based on the current classification. A total of 225 Pectobacterium strains were isolated from 10 main cultivation regions of potato (Solanum tuberosum), napa cabbage (Brassica rapa subsp. pekinensis), and radish (Raphanus sativus) in South Korea; 202 isolates (90%) were from potato, 18 from napa cabbage, and five from radish. Strains were identified using the Biolog test and phylogenetic analysis. The pathogenicity and swimming motility were tested at four different temperatures. Pectolytic activity and plant cell-wall degrading enzyme (PCWDE) activity were evaluated for six species (P. carotovorum subsp. carotovorum, Pcc; P. odoriferum, Pod; P. brasiliense, Pbr; P. versatile, Pve; P. polaris, Ppo; P. parmentieri, Ppa). Pod, Pcc, Pbr, and Pve were the most prevalent species. Although P. atrosepticum is a widespread pathogen in other countries, it was not found here. This is the first report of Ppo, Ppa, and Pve in South Korea. Pectobacterium species showed stronger activity at 28℃ and 32℃ than at 24℃, and showed weak activity at 37℃. Pectolytic activity decreased with increasing temperature. Activity of pectate lyase was not significantly affected by temperature. Activity of protease, cellulase, and polygalacturonase decreased with increasing temperature. The inability of isolated Pectobacterium to soften host tissues at 37℃ may be a consequence of decreased motility and PCWDE activity. These data suggest that future increases in temperature as a result of climate change may affect the population dynamics of Pectobacterium.

• 한국자원식물학회지
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• 제29권5호
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• pp.620-624
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• 2016

본 연구에서 국내 자생식물 추출물중 비만관련 특허가 없는 식물원료를 대상으로 지방세포의 분화억제 효과를 Wnt/β-catenin 신호전달계 활성측정방법으로 탐색하여 비만치료를 위한 기능성소재 응용가능성을 검토하였다. HEK 293-TOP세포의 luciferase 리포터 상대적인 활성은 무 처리 대조군에 비하여 지유(전초), 측백나무(줄기)가 각각 152%, 130%로 높은 활성을 나타내었으며, 피마자(전초)는 약 90%대의 활성을, 해당화(줄기), 괴화(전초)의 경우는 약 80%, 초피나무(줄기), 칡(줄기), 까마중(전초)은 약 70%대의 높은 활성을 나타내었다. 또한, 신경줄기 세포에 대한 어떠한 독성도 보이지 않음으로써 안전한 물질인 것으로 사료되었다. 이 결과는 Wnt/β-catenin 신호전달 활성 측정방법이 향후 High throughput screening 기반기술로 활용될 수 있을 것으로 판단되며, 지방세포 분화 억제활성 후보로 선별된 식물추출물들에 대한 추가적인 실험을 통하여 항비만 소재 개발 응용 가능성을 타진할 것이다.

• 한국작물학회지
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• 제49권4호
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• pp.305-308
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• 2004

Recently, with increasing concern for sustainable agriculture and safe agricultural products, organic farming has become widely adapted as an alternative to conventional farming. This study was conducted to investigate the effects of earthworm casts (EWC) with $100\%$ organic compost on the growth and yield characteristics of fall-cropping potato (Solanum tuberosum L. cv. Dejima) in Jeju. The treatments consisted of seven plots: 2-, 4-, 6-, 8-, and 10-ton $ha^{-1}$ EWC-treated plots, 1.2-ton $ha^{-1}$ complex fertilizer $(N-P_2O_5-K_2O,\;10-10-14)-for-potato$ (CEP)-treated plot as conventional practices, and a control plot. The plant heights were greater in the plots where EWC and CFP were applied than in the control plot. Tuber diameter, number of stems per plant, and chlorophyll level tended to increase in the plots where 8-10 tons $ha^{-1}$ of EWC were applied. The application of CFP and EWC showed an increment in the average tuber weight per plant, but there was minimal significant difference. The application of 8-10 tons $ha^{-1}$ of EWC resulted in an increase in the total tuber yield (21.61-21.87 tons $ha^{-1}$) as compared to the other plots. The highest yield of market able tubers was $69.8\%$ of the total yield from the 10-ton $ha^{-1}$ EWC-treated plot. Consequently, with regard to the growth and yield characteristics of fall-cropping potato, the effects of EWC application were more favorable than the effects of the application of a chemical fertilizer.

• 한국작물학회지
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• 제47권1호
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• pp.68-73
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• 2002

High temperature during sprout emergence period of potato (Solanum tuberosum L.) is a major limitation to the yield of fall-grown potato in Korea. To explore the possibility of improving the yield of fall-grown potato through recycled paper mulching, the changes of soil environment and the growth and yield of potato cv. Daeji as affected by three mulching treatments of non-mulched control, recycled paper mulching (RPM), and black polyethylene film-mulching (BPFM) were examined over two fall seasons at Suwon, Korea. The mulching materials were a recycled mulch paper with 110 g/$m^2$ and a thickness of 0.1 mm, which was manufactured from old corrugated containers for this experiment and a commercial black polyethylene film with a thickness of 0.01mm. On an average throughout the growing period, the soil temperature with RPM at the 5-cm depth was lower by 0.4-1.1$^{\circ}C$ than that of the control. The maximum temperature during daytime in RPM was lowered by approximately 1 to 5$^{\circ}C$ according to the weather condition during emergence period, the difference being great on a fine day. The soil temperature with BPFM was much higher than the control. The effect of the mulching treatments diminished as the canopy became developed. The mulching treatments were more advantageous than the control in the conservation of soil water. Moreover, RPM and BPFM efficiently suppressed the occurrence of weeds until the potato harvest. RPM improved the emergence significantly due to lower soil temperature, whereas BPFM showed much poorer emergence than the control. Growth after emergence and yield were significantly higher in RPM than in the control and BPFM. It was concluded that the significantly improved yield in RPM compared to that in the control was mainly due to the improvement of the sprout emergence and tuber growth accompanied by lower soil temperature and better conservation of soil water.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.153-153
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• 2017

Potato (Solanum tubersosum L.) is susceptible to various environmental stresses such as frost, high temperature, and drought. Enhancement of potato drought tolerance can reduce yield loss under drought that has negative effect on potato tuber growth. Genetic engineering can be utilized to achieve this goal, but such approaches using endogenous potato genes have rarely been applied. Since unpredictable global weather changes cause more severe and frequent water limiting conditions, improvement of potato drought tolerance can minimize such adverse effects under drought and can impact on sustainable potato production. Genetic engineering can be utilized to improve potato drought tolerance, but such approaches using endogenous potato genes have rarely been applied. We were obtained AtbZIP28 gene transgenic potato plants. It is identified transcript levels at various stress conditions, polyethylene glycol (PEG), NaCl, abscisic ${\underline{acid}}$ (ABA). Also, For identification to regulate ER stress response genes in AtbZIP28 gene transgenic potato plant, we screened seven potato genes from RNA-seq analysis under TM treatment. Five and two genes were up- and down-regulated by TM, respectively. Their expression patterns were re-examined at stress agents known to elicit TM, DTT, DMSO and salt stress.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.309-309
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• 2017

The experiments were carried out in open experimental field of Field Crops Research Institute, Hai Duong, Vietnam in Winter Spring seasons 2015-2016 and 2016-2017 for finding high yielding of Korean chipping potato varieties which are adaptable to Vietnam condition. Six varieties of Korean potato (G1, G2, G4, G10, B1, B2) were experimented. The control variety (G6) was Atlantic which has long been a standard chip variety. The results showed that, for a hill, there was positive correlation between number of stems and number of tubers. The hill yield also showed highly relation to the plant height and number of tubers. Even though the growth and yield of potatoes grown in the season 2016-2017 were significant higher than these of potatoes grown in the season 2015-2016, their percentages of tubers infected by scab disease were much higher. This is probably because of irregular soil moisture content at the tuber forming duration. While G1, G6 and B1 are white flesh varieties, the remaining are yellow flesh varieties. There are two shapes types among experimented varieties: oblong (G1, G10, B1) and round shape (G2, G4, G6, B2). The total yields of varieties G1, G2, B1, B2 were higher than the total yield of the control variety from 16.5-49.4% in the season 2015-2016 and from 39.8-75.6% in the season 2016-2017.