• Current Research on Agriculture and Life Sciences
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• v.15
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• pp.109-114
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• 1997

This studies were conducted to improve the root formation of plantlet derived from shoot tip culture and to evaluate the optium transplanting date of Cnidium officinale Makino in field. The rooting rate of shoot-tip derived plantlets was 81% on media containing 1.0 mg/L IBA and 0.05 mg/L BA within 30 days after culture. Upon transfer into potting soil, the seedling grown well under 75% shading. Optimal transplanting date on taking roots and rhizome growth was May 5 in field.

• Journal of the Korean Institute of Landscape Architecture
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• v.33 no.2 s.109
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• pp.71-82
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• 2005

A large scale of riverside rearrangement has been recently done in the major rivers in Korea. So inundation possibility in agricultural area closed by these rivers has been higher than the possibility a few years ago. However, land use in this area has not been adjusted to a change of this situation near the rivers. Therefore, when typhoon or heavy rain is happened on this area, it can cause a large damage in agricultural area. This study analyzed inundation potentiality in agricultural area at Ibang-myeon, Changnyeong-gun, Kyeongnam-province, Korea by using the logistic regression model and the piecewise regression model. The first thing we did was to transfer the inundation area per elevation to the accumulated inundation area per elevation. This accumulated inundation area per elevation as an distribution function could be described by the logistic regression model(LRM), and piecewise regression model(PRM) could make it much more accurate to analyze the inundation area per elevation. As a result, the regression models derived from LRM and PRM showed $R^2$ over 0.950. The models derived from LRM and PRM in Ibang-myeon noted that frequently inundated area(FIA) was shown up to 12.12m in elevation, and potentially inundated area(PIA) was shown up to 14.60m in elevation. In FIA, regular agricultural activity would be impossible. And It would be not easy to continue the regular agricultural activity in PIA. So, this land should be rearranged to be used for a buffer zone for ecosystem protection, landscape conservation and things like that in riverside.

• Proceedings of the Korean Geotechical Society Conference
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• 2004.03b
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• pp.28-35
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• 2004

Driven pile has the excellent bearing characteristics and good economics, so it is known as the comparative piling method. To use the advantages of driven pile fully, it is necessary to perform the proper construction management. Engineers must drive pile to the proper bearing layer with proper blow energy and measure the blow count and penetration per certain depth to analyze the bearing capacity and driveability. In conventional method, these parameters have been measured manually so it was difficult to get good accuracy. After PIR-D(Pile Installation Recorder-Driven Pile) was attached to the driving equipment, the hammer efficiency, blow count and penetration in blow/10cm were measured automatically. In this paper, to givethe rational judgement criteria of bearing layer, driveability, blow/10cm according to pile depth during pile driving, the some relationship between the driving resistance and ground layer distribution was analyzed. The ground investigation during piles (PHC ${\Phi}450,\;{\Phi}400\;&\;Steel\;Pile\;{\Phi}609{\ast}16t$) installation in the marine clay layer in Incheon, the sandy soil layer in Yongin and the tuff layer in Pusan was done. And measuring hammer efficiency not doing recently, we could compare hammer efficiency(Eh) by PIR-D and energy transfer ratio(ETR) by Pile Dynamic Analyzer(PDA).

• Journal of Plant Biotechnology
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• v.5 no.4
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• pp.221-224
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• 2003

Leaf explants of Ixeris sonchifolia produced adventitious shoots at a frequency of 100% when cultured on MS medium supplemented with combinations of various concentrations of 6-benzyladenine (BA) (0.44, 4.44, or 8.87 ${\mu}M$) and 0.54 ${\mu}M$ NAA, or MS medium supplemented with 22.19 ${\mu}M$ BA and 2.69 ${\mu}M\;\alpha$-naphthaleneacetic acid (NAA) after four weeks of culture. Each explants (approximately $3{\times}6mm$) produced greater than 70 shoots at a combination of 0.44 ${\mu}M$ BA and 0.54 ${\mu}M$ NAA. Leaf explants produced shoots at a frequency of greater than 80% even at as low as 0.13 ${\mu}M$ BA as the sole growth regulator. Upon transfer to one-third strength MS with 0.54 ${\mu}M$ NAA, excised adventitious shoots were rooted at a frequency of 100%. Regenerated plantlets were transplanted to potting soil and grown to maturity in a greenhouse. The competence of I. sonchifolia for plant regeneration via organogenesis appears to be greater than the competence of tobacco, currently the best model plant for organogenesis.

• Journal of Plant Biotechnology
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• v.5 no.4
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• pp.225-231
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• 2003

The hormonal requirement suiting micropropagation of Morus alba during any season throughout the year was studied. Sprouting frequency from axillary buds of M. alba was greatly influenced by the time of explant collection, the highest value was achieved when nodal explants were collected at the end of bud dormancy period (late in March) and cultured on Murashige and Skoog (MS) medium supplemented with low concentration (0.5 mg/L) of BAP, kinetin or IBA (85-68%). In addition, they showed higher axillary bud sprouting on growth-regulators-free medium (49%) than others collected in autumn or winter and cultured on medium supplemented with various growth regulators (47-48%). Regardless of that period, young explants with greenish buds collected in summer exhibiting high sprouting frequency (66%) on MS medium supplemented with 0.5 mg/L kinetin and 0.5 mg/L GA3. Shoot multiplication via adventitious bud formation was achieved when the nodal explants were cultured on MS medium supplemented with 2 mg/L BAP and 0.2 mg/L IBA. Further multiplication via nodal explants of in vitro grown shoots was obtained on MS medium supplemented with 0.5 mglL BAP and 0.5 mg/L GA3. While half strength MS medium supplemented with low concentration (0.5 mg/L) of IBA, IAA or 2,4-D stimulated adventitious root formation, IBA was the best. After transfer the plantlets to the soil, acclimatization for three weeks was essential prerequisite for survival in high frequency (92%). Peroxidase activity is related to break of bud dormancy where maximum enzyme activity was detected when the lateral buds were induced to commence growth under field condition (early in spring) or in vitro.

• Journal of Plant Biotechnology
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• v.36 no.2
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• pp.193-196
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• 2009

Pinellia koreana K-H Tae & J-H Kim is a recently discovered Korea endemic medicinal plant species whose natural habitat is rapidly destroyed by industrial development. Described in this paper are culture conditions for high frequency plant regeneration via bulblet formation from leaf explant cultures of P. koreana. Leaf explants formed white nodular structures and off-white calluses at a frequency of 91.2% when cultured on MS medium supplemented with 2 mg/L BA and 0.5 mg/L NAA. However, the frequency of white nodular structures and off-white calluses formation was slightly decreased with an increasing concentration of NAA up to 4 mg/L, where the frequency reached 31.7%. Most petiole explants did not form white nodular structures and off-white calluses except the combination treatment of 2 mg/L BA and 2 mg/L NAA. Upon transfer onto MS basal medium, over 90% of nodular structures gave rise to numerous bulblets and developed into plantlets. Plantlets regenerated from bulblets were transplanted to potting soil and grown to maturity at a survival rate of over 95% in a growth chamber. Therefore, the in vitro plant regeneration system of P. koreana obtained in this study will be useful for mass propagation and long-term preservation of genetic resources of P. koreana.

• Journal of Plant Biology
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• v.35 no.2
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• pp.135-142
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• 1992

Shoot tip explants from germinated seeds of Alnus hirsuta were cultured on NT (Nagata and Takebe, 1971) mineral salts medium supplemented with 6% glucose, MS (Murashige and Skoog, 1962) vitamin mixture, polyvinylpyrrolidone (PVP) and $0-50\;\mu\textrm{M}$ 6-benzylaminopurine (BAP). Five $\mu\textrm{M}$ BAP was found to give the highest shoot multiplication rate. Accordingly about 200 shoots were obtained for further experiments by multiplying shoots on this medium for 4-5 months. Regardless of carbon sources, NT mineral medium produced 3-12 times of shoots than MS mineral medium did. On NT mineral medium, 3% sucrose, 3% glucose and 6% glucose yielded no significant differences. It was observed that media consisting of 1/4-1/2 strength NT mineral salts, 3% sucrose and $1-8\;\mu\textrm{M}$ IBA produced about 100% rooting rate. Almost 100% of the resulting plantlets survived after transfer to the soil by decreasing humidity stepwise.epwise.

• Korean Journal of Plant Tissue Culture
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• v.21 no.1
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• pp.1-6
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• 1994

Cotyledonary and hypocotyl explants of melon seedlings were cultured on Murashige and Skoog's (MS) medium supplemented with various concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and benzyladenine (B.A).Up to 22% of cotyledonary explants and 7%, of hypocotyl explants, respectively: Produced somatic embryos through intervening two types of calli: bright yellow compact (BYC) callus and pale-yellow compact (PYC) callus. BYC callus was capable of producing somatic embryos at initial culture, but it became necrotic as subrulhues proceeded. In contrast UC callus was incapable of producing somatic embryos during initial culture (first 6 weeks), but it became bright-yellow friable (BYF) callus with forming a few globular embryos after 2 months of subculture, indicating that the callus turned embryogenic. The embryogenic capacity of BYF maintained for over one year when the callus was sucultured at 4-week interval. Upon transfer onto MS basal medium the callus gave rise to numerous somatic embryos and subsequently converted to plantlets. Plantlets were transplanted to potting soil and grown to maturity in the phyotron.

• Journal of Plant Biotechnology
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• v.43 no.2
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• pp.240-247
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• 2016

In this study, routinely used transformation method, which includes transferring explants onto co-cultivation medium after inoculating them with bacterial solution for a while, was compared with 3 different inoculation methods. In every 3 methods, hypocotyl explants excised from 7-day-old sterile flax seedlings having cotyledon leaves and no root system dried under air flow in sterile cabin for 35 min were inoculated with different volumes of bacterial solution at different inoculation periods. GV2260 line of Agrobacterium tumefaciens having 'pBIN 19' plasmid containing npt II (neomycin phosphotransferase II) gene and GUS reporter gene was used in transformation studies. After inoculation, hypocotyl segments of seedlings (0.5 cm in length) - were excised and left to co-cultivation for 2 days. Then, explants were transferred to regeneration medium supplemented with different antibiotics. The presence of npt-II and GUS genes in transformants was confirmed by PCR and GUS analysis. The highest results in all characters examined in all cultivars were obtained from the 2 inoculation method in which hypocotyls excised from seedlings inoculated with $500{\mu}l$ of bacterial solution after drying in sterile cabin for 35 min were used.

• Journal of the Korean Geotechnical Society
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• v.19 no.4
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• pp.223-233
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• 2003

Sand compaction pile (SCP) is one of the ground improvement techniques which are being used for not only accelerating consolidation but also increasing bearing capacity of loose sands or soft clay grounds. In this study, laboratory model tests and 3-D finite element analyses were performed to investigate the interaction between sand compaction piles and surrounding soft soils. Based on the results obtained, as the area replacement ratio increases, the stress concentration ratio increases at the pile point, the settlement decreases, and the relative displacement between column and soil also decreases. It is also found that numerical study is illustrated by good comparison with model test results, and the numerical analysis revealed slip effects which could not be specifically identified in the model tests.