• Journal of Korea Water Resources Association
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• v.34 no.5
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• pp.533-542
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• 2001

In order to establish drought policy, the estimation of drought period for each drought situation should be preceded. Non-linear Water Balance Model(NWBM) and palmer Drought Severity Index (PDSI) can be used for analysis of drought period. As a water balance method considering moisture transfer between land surface and atmosphere, NWBM can be used to estimate transition time between dry and wet period induced by stochastic fluctuations. PDSI is also water balance method to show drought severity comparing actual precipitation with climatically appropriate precipitation based on precipitation and potential evapotranspiration. In this study, the drought periods are estimated using NWBM and PDSI for the Han River Basin. The drought periods according to the soil moisture estimated by NWBS and the drought periods according to drought severity index estimated by PDSI show similar trend. The estimated drought period from extreme drought to wet condition for the Han River Basin is about 3years.

• Journal of Plant Biotechnology
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• v.29 no.4
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• pp.253-257
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• 2002

To establish the optimum condition for plant regeneration from somatic embryos of Acanthopanax sessiliflorus Rupr. et Maxim, a medicinal plant, somatic embryos were induced from zygotic embryo-derived embryogenic callus in hormoen-free MS medium. To induce plantlet conversion, cotyledonary somatic embryos were cultured on MS solid medium with GA$_3$at various concentrations (0~10 mg/L) for three weeks. Plantlets were transferred to 1/3 MS solid medium with 0.5% charcoal for 7 weeks. Stem length was increased proportionally to the concentration and treatment period of GA$_3$. Also, the highest leaf width (8.9 mm) and leaf number (2.84) of plantlet were obtained when plantlets were converted on 5,10 mg/L GA$_3$pretreatments, respectively. The highest plant conversion frequency (66.7%) was obtained when the somatic embryos were cultured on medium containing 5 mg/L GA$_3$ for 3 weeks and then were transferred to 1/3 MS medium with 0.5% charcoal. The highest survival rate of soil transfer was 90% when plantlets were regenerated on medium with 5 mg/L GA$_3$ for 3 weeks and then transferred to plastic pots containing vermiculite and sand mixture for 4 weeks.

• Korean Journal of Plant Tissue Culture
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• v.24 no.6
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• pp.369-374
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• 1997

Immature flower buds of 'Desio' carnation were cultured on MS agar medium supplemented with 1 ㎎/L 2,L-D. Embryogenic calli were formed from 5-10% of the buds less than 20 ㎜ in length, but only non-embryogenic calli were produced from explants of shoot apex leaf, internode, and flowere buds larger than 20 ㎜. The same method was applied to 16 cultivars of cut Sower carnation and embryogenic calli were obtained in 7 cultivars. Several embryogenic callus lines were selected and maintained through subcultures over 120 weeks without loss of embryogenic competence. The embryogenic cultures were also proliferated rapidly in liquid agitation cultures using MS medium supplemented with 1mg/L 2,4-D. Numerous embryos were formed on the periphery of the cell aggregates upon transfer to auxin-free MS agar medium. Plantlets were transplanted in potting soil and grown to bloom in six months.

• Korean Journal of Plant Tissue Culture
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• v.25 no.5
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• pp.329-333
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• 1998

$\beta$-Glucuronidase (GUS) gene of Escherichia coli was introduced into sweet potato (Ipomoea batatas (L.) Lam.) cells by particle bombardment and expressed in the regenerated plants. Microprojectiles coated with DNA of a binary vector pBI121 carrying CaMV35S promoter-GUS gene fusion and a neomycin phosphotransferase gene as selection marker were bombarded on embryogenic calli which originated from shoot apical meristem-derived callus and transferred to Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid and 100 mg/L kanamycin. Bombarded calli were subcultured at 4 week intervals for six months. Kanamycin-resistant calli transferred to MS medium supplemented with 0.03 mg/L 2iP, 0.03 mg/L ABA, and 50 mg/L kanamycin gave rise to somatic embryos. Upon transfer to MS basal medium without kanamycin, they developed into plantlets. PCR and northern analyses of six regenerants transplanted to potting soil confirmed that the GUS gene was inserted into the genome of the six regenerated plants. A histochemical assay revealed that the GUS gene was preferentially expressed in the vascular bundle and the epidermal layer of leaf, petiole, and tuberous root.

• Proceedings of the Korea Water Resources Association Conference
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• 2007.05a
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• pp.15-21
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• 2007

Due to a decreasing tendency of river runoff in the Laohahe River basin in North China, quantitative analysis was made with the aid of the conceptual Xinanjiang model under the background of nature climate variability as well as human-induced climate change according to the long-term observational hydrometeorological data. In the past, the human effect on surface water resources was estimated by investigating the impact of human activities on each item in the equation of water balance, so as to calculate water quantity of each item in the original natural status. It seems to be clear conceptually. It is appropriate just for the case of direct impact, such as water transfer from one basin to another, water storage by various scales of hydraulic projects, besides a huge amount of investigation and indeterminate statistics data when applied in practice. It is difficult for us to compute directly water consumption due to the implementation of measures for soil conservation, the improvement of farming techniques in agriculture, the growth of population in towns and villages, and the change of socioeconomic structure. In view of such situation, the Xinanjiang model was used to separate human impact from the climatic impact on water resources. Quantitatively human activity made river runoff decrease by 1.02, 50.67, 58.06 mm in 1960's, 1970's, 1980's, respectively, while by 97.2 mm in 1990's in the sense of annual average in the Laohahe River basin.

• Journal of The Korean Society of Agricultural Engineers
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• v.53 no.4
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• pp.67-74
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• 2011

The objectives of this study were to assess the rice yields and evaluate fertilizer reduction effect of reclaimed wastewater irrigation in paddy fields using the Decision Support System for Agrotechnology Transfer (DSSAT) v4.5 model. The experimental plots were designed, which was located near the Suwon wastewater treatment plant in Gyeonggi-do, Korea. The rice yield, irrigation amount, irrigation water quality and soil data were monitored and collected between 2006 and 2009. The DSSAT model was calibrated and validated with observed data. The methods that were used to evaluate this model were the root mean square error (RMSE), normalized root mean square error (nRMSE), and index of agreement (d). The values of RMSE, nRMSE, and d ranged from 145 to $789\;kg\;ha^{-1}$, 3.0 to 13.3 %, and 0.90 to 0.95 for the calibration period, respectively and represented from 91 to $538\;kg\;ha^{-1}$, 2.0 to 10.4 %, 0.94 to 0.98 for the validation period, respectively. Overall, this model showed good agreement with observed data of rice yields irrigated with reclaimed wastewater. The fertilizer reduction effect in paddy field of reclaimed wastewater irrigation was assessed about 60 % in 2008 and 40 % in 2009.

• Microbiology and Biotechnology Letters
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• v.38 no.4
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• pp.475-480
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• 2010

Actinomycetes are Gram-positive soil bacteria and one of the most important industrial microorganisms due to superior biosynthetic capabilities of many valuable secondary metabolites as well as production of various valuable bioconversion enzymes. Among them are cytochrome P450 hydroxylase (CYP), which are hemoproteins encoded by a super family of genes, are universally distributed in most of the organisms from all biological kingdoms. Actinomycetes are a rich source of soluble CYP enzymes, which play critical roles in the bioactivation and detoxification of a wide variety of metabolite biosynthesis and xenobiotic transformation. Cyclosporin A (CyA), one of the most commonly-prescribed immunosuppressive drugs, was previously reported to be hydroxylated at the position of 4th N-methyl leucine by a rare actinomycetes called Sebekia benihana, leading to display different biological activity spectrum such as loss of immunosuppressive activities yet retaining hair growth-stimulating side effect. In order to improve this regio-selective CyA hydroxylation in S. benihana, previously-identified several secondary metabolite up-regulatory genes from Streptomyces coelicolor and S. avermitilis were heterologously overexpressed in S. benihana using an $ermE^*$ promoter-containing Streptomyces integrative expression vector. Among tested, SCO4967 encoding a conserved hypothetical protein significantly stimulated region-specific CyA hydroxylation in S. benihana, implying that some common regulatory systems functioning in both biosynthesis and bioconversion of secondary metabolite might be present in different actinomycetes species.

• Journal of the Korean Geotechnical Society
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• v.21 no.5
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• pp.65-74
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• 2005

The negative skin friction on piles, which are installed in currently consolidating soft deposits, creates significant problems on the stability of pile foundations. This study investigated whether or not the pile foundation designs were appropriate in soft deposits with large amount of consolidation settlement. The final settlements of the grounds along the pile depth were estimated by the soil parameters obtained from the laboratory tests and by the field-measured settlement curves, if they were available. The displacement of the piles along the pile depth was estimated by both the load transfer method and the numerical method. Both methods gave similar locations of neutral planes and magnitudes of the maximum axial forces on the piles. The movements of the ground and the piles were compared to calculate the down drag acting on piles. For the piles whose bearing capacities were less than the design loads including the down drag, slip layer coatings and/or incrementing of the pile penetration depth into the bearing stratum were proposed to improve the pile capacities.

• Journal of the Korean Geotechnical Society
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• v.29 no.5
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• pp.5-17
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• 2013

This study investigates the effect of thermal stress on axial load and pile settlement of PHC energy piles. A series of numerical analyses were performed by controlling major influencing parameters such as pile arrangement, pile spacing, end-bearing condition, soil condition and pile cap stiffness. It is found that the characteristics of pile-load transfer are significantly affected by seasonal operation mode (i.e., cooling and heating) throughout the year. Also, the axial load under thermal loading increases with increasing the pile spacing. The settlement of the pile in sand is larger than that in clay because of the thermal stress generated. It is also found that thermal stress highly influences on the end-bearing pile, corner pile and rigidity of pile cap.

• Journal of the Korean Geotechnical Society
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• v.29 no.9
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• pp.31-41
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• 2013

The use of energy pile foundation has increased for economic utilization of geothermal energy. In particular, coil-shaped ground heat exchanger (GHE) is preferred to the conventional U-shaped exchanger to ensure better efficiency of heat exchange rate. This paper presents a numerical and experimental study on the heat transfer behavior of PHC energy piles. Field thermal performance tests (TPTs) were conducted for the PHC energy piles installed in a partially saturated weathered granite soil deposit, in which two types of GHEs were considered: W and coil shaped GHEs. Besides, three-dimensional finite element analyses were also conducted, and the results were compared with the experimental results. According to the results of TPT and numerical analyses, the coil shaped GHE showed 10~15% higher heat exchange rate than the W type GHE in the PHC energy piles.