• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.2
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• pp.198-208
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• 1992

Recently, a nursery mat made from rock wool has realized transplanting of the younger seedlings with the ordinary transplanting machines for Chibyo and Chubyo(3 and 4～5 leaved seedling, respectively). The seedlings defined as the 'Nyubyo' or 'Nursling seedlings' became possible to achieve economic profits from the reduction in both working time and costs. It being widely noticed as a strategy to solve the difficulties in current rice cultivation. The nursling seedlings are 1.4 to 2.5 leaves and height at 4.5 to 7cm, grown 4 to 7 days after seeding. They maintain still up to 50 to 80% of their nutrients in the endosperm, and can grow by using only their own nutrients for a certain period of time after transplanting. Nursling seedlings take 2 days in the nursery chamber at 32$^{\circ}C$ after seeding, and 2 days in the greening house at $25^{\circ}C$. This is only 4 days, all together, to make the nursling seedlings of 1.5 leaves which are ready for transplanting. Watering is only needed once at the sowing time. It only takes 1 or 2 waterings even to raise a seedlings for a period of 7 days. The number of nursery boxes can be reduced because it is possible to sow more densely(220 to 240g per box), thus it only needs seedlings of 15 to 16 boxes per 10 a which leads to a reduction in facilities and space needed. Temperature during the nursery period can be artificially adjusted more precisely which may lead to the prevention of temperature stress. The nursling seedlings can root rapid by because the crown roots from the coleoptile node begin to emerge immediately after transplanting. They show strong resistance to low temperature (12$^{\circ}C$) and deep-planting. There is no danger in the rooting of the seedlings even if half of their height is buried into the soil. Moreover, it can root at a rate of up to 65 to 80% even if the full height of the seedlings is buried. They show also strong resistance to submergence (10～15cm). The nursling seedlings tend to grow by producing tillers from lower nodes. It is therefore, necessary to control to keep the proper numbers of tillers per unit area. They have no fear in the delay of heading and their yield components can be so well balanced that the same level of yield was achieved with the nursling seedlings compared to that with Chibyo. It was further suggested that if the surplus tillers can be avoided by such cultivation practices, the number of grain per panicle can be kept greater and higher yield can be realized. Practical experiments with the nursling seedlings conducted in 1989 and 1990 by farmers in various areas showed exciting results. The nursling seedlings will become widely spread, or at least occupy an important position in Japanese and also in Korean rice cultivation techniques.tivation techniques.

• Journal of the Korea institute for structural maintenance and inspection
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• v.14 no.4
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• pp.70-77
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• 2010

Microcement grouting and micro pile are frequently used for ground modification during tunnel construction. The influence of grouting pressure of microcement grouting and micro pile to the existing bridge which is directly over the constructing tunnel is investigated. Three dimensional seepage flow-structure interactive analysis considering firm water pressure with full stages of construction including the construction of upper bridge, microcement grouting, micro pile and tunnel is performed. The settlement and tilting of the pier of existing bridge violate the design code and the reaction of the bridge are highly increased after grouting. The stress of tunnel bracings such as rockbolt and shotcrete also exceed the limit of the code. The pressure of microcement grouting is confined by bedrock and transmit to the surrounded soil and the upper bridge. Microcement grouting needs mid-high pressure to penetrate through weak fault plane and the pressure greatly influence the safety of the upper structure. It is important to decide and care the grouting pressure to improve weak fault plane directly under the existing structures and the pressure of microcement grouting should be considered in underground analysis.

• Journal of Life Science
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• v.25 no.2
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• pp.189-196
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• 2015

Bacterial diversity was studied in the rhizosphere of Suaeda japonica Makino, which is native to Suncheon Bay in South Korea. Soil samples from several sites were diluted serially, and pure isolation was performed by subculture using marine agar and tryptic soy agar media. Genomic DNA was extracted from 29 pure, isolated bacterial strains, after which their 16S rDNA sequences were amplified and analyzed. Phylogenetic analysis was performed to confirm their genetic relationship. The 29 bacterial strains were classified into five groups: phylum Firmicutes (44.8%), Gamma proteobacteria group (27.6%), Alpha proteobacteria group (10.3%), phylum Bacteriodetes (10.3%), and phylum Actinobacteria (6.8%). The most widely distributed genera were Bacillus (phylum Firmicutes), and Marinobacterium, Halomonas, and Vibrio (Gamma proteobacteria group). To confirm the bacterial diversity in rhizospheres of S. japonica, the diversity index was used at the genus level. The results show that bacterial diversity differed at each of the sampling sites. These 29 bacterial strains are thought to play a major role in material cycling at Suncheon Bay, in overcoming the sea/mud flat-specific environmental stress. Furthermore, some strains are assumed to be involved in a positive interaction with the halophyte S. japonica, as rhizospheric flora, with induction of growth promotion and plant defense mechanism.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.28 no.4
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• pp.177-190
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• 2016

In case of the seabed around and under gravity structures such as submerged breakwater is exposed to a large wave action long period, the excess pore pressure will be generated significantly due to pore volume change associated with rearrangement soil grains. This effect will lead a seabed liquefaction around and under structures as a result from decrease in the effective stress. Under the seabed liquefaction occurred and developed, the possibility of structure failure will be increased eventually. Lee et al.(2016) studied for regular waves, and this study considered for irregular waves with the same numerical analysis method used for regular waves. Under the condition of the irregular wave field, the time and spatial series of the deformation of submerged breakwater, the pore water pressure (oscillatory and residual components) and pore water pressure ratio in the seabed were estimated and their results were compared with those of the regular wave field to evaluate the liquefaction potential on the seabed quantitatively. Although present results are based on a limited number of numerical simulations, one of the study's most important findings is that a more safe design can be obtainable when analyzing case with a regular wave condition corresponding to a significant wave of irregular wave.

• Korean Journal of Plant Resources
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• v.18 no.1
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• pp.15-21
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• 2005

Korean ginseng(Panax ginseng C.A. Meyer) is very difficult to obtain stable production of qualified ginseng roots because of variable stresses in soil environments. In transformation of ginseng with betain aldehyde dehydrogenase gene, compounds synthesized for controlling osmotic pressure such as proline, glycine, betaine, polyols and sugar were accumulated in cell for salt resistance in transgenic plants. 2 Agrobactgerium conjugants were acquired with bet A and bet B genes for solt resistant plants. A. tumefaciens MP90/pBetA and A. tumefaciens MP90/pBetB were recombined for increasing the tolerance to salt stress. To confirm the transformation of the binary vector, tobacco plant was transformed, and the transformant can grow on media containing high concentration of kanamycin. To identify NPT 11, BetA and BetB genes of the transformants, the band on the agarose was confirmed by PCR and RT-PCR techniques. The transformants of ginseng with bet A and bet B genes were acquired on the phytohormone free basic MS media containing only antibiotics and 1M mannitol used for selection of transgenic plant, but the transfomation efficiency for BetA and BetB was very low.

• Molecules and Cells
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• v.40 no.3
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• pp.230-242
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• 2017

In the Arabidopsis genome, approximately 80 MAP3Ks (mitogen-activated protein kinase kinase kinases) have been identified. However, only a few of them have been characterized, and the functions of most MAP3Ks are largely unknown. In this paper, we report the function of MAP3K16 and several other MAP3Ks, MAP3K14/15/17/18, whose expression is salt-inducible. We prepared MAP3K16 overexpression (OX) lines and analyzed their phenotypes. The result showed that the transgenic plants were ABA-insensitive during seed germination and cotyledon greening stage but their root growth was ABA-hypersensitive. The OX lines were more susceptible to water-deficit condition at later growth stage in soil. A MAP3K16 knockout (KO) line, on the other hand, exhibited opposite phenotypes. In similar transgenic analyses, we found that MAP3K14/15/17/18 OX and KO lines displayed similar phenotypes to those of MA3K16, suggesting the functional redundancy among them. MAP3K16 possesses in vitro kinase activity, and we carried out two-hybrid analyses to identify MAP3K16 substrates. Our results indicate that MAP3K16 interacts with MKK3 and the negative regulator of ABA response, ABR1, in yeast. Furthermore, MAP3K16 recombinant protein could phosphorylate MKK3 and ABR1, suggesting that they might be MAP3K16 substrates. Collectively, our results demonstrate that MAP3K16 and MAP3K14/15/17/18 are involved in ABA response, playing negative or positive roles depending on developmental stage and that MAP3K16 may function via MKK3 and ABR1.

• Journal of the Korean Geotechnical Society
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• v.18 no.5
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• pp.37-42
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• 2002

Artificial neural networks are efficient computing techniques that are widely used to solve complex problems in many fields. In this paper a liquefaction potential was estimated by using a back propagation neural network model applicated to cyclic triaxial test data, soil parameters and site investigation data. Training and testing of the network were based on a database of 43 cyclic triaxial test data from 00 sites. The neural networks are trained by modifying the weights of the neurons in response to the errors between the actual output values and the target output value. Training was done iteratively until the average sum squared errors over all the training patterns were minimized. This generally occurred after about 15,000 cycles of training. The accuracy from 72% to 98% was shown for the model equipped with two hidden layers and ten input variables. Important effective input variables have been identified as the NOC,$D_10$ and (N$_1$)$_60$. The study showed that the neural network model predicted a CSR(Cyclic shear stress Ratio) of silty-sand reasonably well. Analyzed results indicate that the neural-network model is more reliable than simplified method using N value of SPT.

• Journal of the Korean GEO-environmental Society
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• v.21 no.7
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• pp.17-26
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• 2020

The soft ground in the southwest coastal area composed of marine clay is greatly influenced by sediment composition, particle size distribution, particle shape, adsorption ions and pore water characteristics, tide and temperature. In addition, the geotechnical properties are very complex due to stress history, change in pore water, dissolution process and gas formation. In this study, the physical and mechanical properties of the soft ground were evaluated through field tests and laboratory tests to investigate the strength increase characteristics according to consolidation on the soft ground in the southwest coast. In addition, in order to understand the consolidation behavior of soft ground such as subsidence, pore water pressure, horizontal displacement of soil by embankment load, measuring instruments such as pore water pressuremeter, settlement gauge, inclinometer and differential settlement gauge was installed, and a piezocon penetration test was carried out step by step to confirm the increase in shear strength of the ground. Through this, it was confirmed that the shear strength of the ground is increased according to the stages of filling. In addition, by evaluating the properties of consolidation behavior, strength increase and consolidation prediction by empirical methods and theories were compared to analyze the characteristics of strength increase rate and consolidation behavior in consideration of regional characteristics.

• Journal of Sensor Science and Technology
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• v.24 no.5
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• pp.291-298
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• 2015

There are many landslides occurred by typhoons and intense rainfall during the summer seasons in Korea. To predict a landslide triggering it is important to understand mechanisms and potential areas of landslides by the geological approaches. However, recent climate changes make difficult to predict landslide based on only conventional prediction methods. Therefore, the importance of a real-time monitoring of landslide using various sensors is emphasized in recent. Many researchers have studied monitoring techniques of landslides and suggested several monitoring systems which can be applicable to the natural terrain. Most sensors of landslide monitoring measure slope displacement, hydrogeologic properties of soils and rocks, changes of stress in soil and rock fractures, and rainfall amount and intensity. The measured values of each sensor are transmitted to a monitoring server in real-time. The ultimate goal of landslide monitoring is to warn landslide occurrence in advance and to reduce damages induced by landslides. This study introduces the current situation of landslide monitoring techniques in each country.

• Journal of Korean Society of Environmental Engineers
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• v.34 no.1
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• pp.63-71
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• 2012

As nanotechnology is a key industry, there is growing concern relating to the potential risk of nanoparticles. They are known to be released into the environment via various exposure routes. When nanoparticles are present in water environments, they are supposed to be illuminated by ultraviolet light, and the ecotoxicity of photoactive nanoparticles may be changed. In this study, a review of the ecotoxicity of photoactive nanoparticles, including the mechanisms of phototoxicity, are presented. In order to address this issue, studies on the ecotoxicity to soil and water organisms exposed to photoactive nanoparticles were investigated. The photoactive nanoparticles chosen for this study were zinc oxide, titanium dioxide and fullerene. Microorganisms, nematode, earthworm, algae and fish, etc., were chosen to assess the toxicity of nanoparticles using diverse methods. However, studies on the phototoxicity potentially induced by nanoparticles on UV illumination have been reviewed in only 8 studies. From a few studies, photoactive nanoparticles have shown high dissolution rates under UV conditions, with the released ions observed to profoundly influence test organisms. In addition, NPs exposed to UV produced reactive oxygen species (ROS). These ROS can induce oxidative stress in exposed organisms. Evidence of phototoxicity by nanoparticles were found based on previous studies.