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Ginseng Transformation of Betaine Aldehyde Dehydrogenase Gene Relative Salt Resistant through Somatic Embryogenesis  

Yoon Young-Sang (College of Industrial Sciences, Kongju National University)
Bae Chang-Hyu (College of Agriculture and Life Sciences, Sunchon National University)
Song Won-Seob (College of Agriculture and Life Sciences, Sunchon National University)
Yoon Jae-Ho (Institute of KuRae Wild Flower)
Yang Deok-Chun (College of Life Science, Kyung Hee University)
Publication Information
Korean Journal of Plant Resources / v.18, no.1, 2005 , pp. 15-21 More about this Journal
Abstract
Korean ginseng(Panax ginseng C.A. Meyer) is very difficult to obtain stable production of qualified ginseng roots because of variable stresses in soil environments. In transformation of ginseng with betain aldehyde dehydrogenase gene, compounds synthesized for controlling osmotic pressure such as proline, glycine, betaine, polyols and sugar were accumulated in cell for salt resistance in transgenic plants. 2 Agrobactgerium conjugants were acquired with bet A and bet B genes for solt resistant plants. A. tumefaciens MP90/pBetA and A. tumefaciens MP90/pBetB were recombined for increasing the tolerance to salt stress. To confirm the transformation of the binary vector, tobacco plant was transformed, and the transformant can grow on media containing high concentration of kanamycin. To identify NPT 11, BetA and BetB genes of the transformants, the band on the agarose was confirmed by PCR and RT-PCR techniques. The transformants of ginseng with bet A and bet B genes were acquired on the phytohormone free basic MS media containing only antibiotics and 1M mannitol used for selection of transgenic plant, but the transfomation efficiency for BetA and BetB was very low.
Keywords
Bet A; Bet B; mannitol; Embryo culture; Panax ginseng; salt resistance;
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