• Journal of The Korean Society of Grassland and Forage Science
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• v.20 no.2
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• pp.139-146
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• 2000

A trial was carried out to investigate the effect of fermented saw-dust pig manure (FSP) and N fertilizer application on physical, chemical properties and microbial population of soil on Cheju brown volcanic ash pasture during the period from September, 1997 to January, 1999. Average soil N contents during 3 different periods, August and October, 1998, and January, 1999 were 0.39, 0.41 and 0.39% for fertilizer N level 0, 150 and 300kg/ha, respectively. Soil N contents determined in January, 1999, was significantly increased by an increase of fertilizer N. Nitrogen contents in the soil applied with 0, 3, 6 and 12 tons 1 ha of FSP were 0.43, 0.40, 0.38 and 0.38%, respectively, showing decreasing tendency of soil with increasing levels of FSP application. Soil N contents determined in August, 1998 and January, 1999, were significantly decreased by increasing levels of FSP application. Organic matter contents of the soil applied with N 0, 150 and 300 kg/ha was 8.04, 8.37, and 9.08%. Soil organic matters determined on the 1st and 2nd period trended to increase with increasing level of fertilizer N. FSP application significantly decreased organic matter contents of pasture soil, (9.14, 8.79, 8.28 and 7.78% OM in soil applied with FSP 0, 3, 6 and 12 tonlha of FSP), respectively. Soil OM determined in October, 1998, also showed a significant decrease with increasing level of FSP. Soil pH, available phosphorus, exchangeable K, Ca, Mg and Na in soil studied were not influenced by fertilizer N or FSP application during all three periods. N application tended to increase soil bacteria count, ($27.0\;{\times}\;10^4\;cfu/g,\;29.4\;{\times}\;1-^4\;cfu/g$ and $53.6\;10^4\;cfu/g$ in the soil applied with 0, 150 and 300 kg N/ha, respectively). The number of colonies of soil bacteria and fungi determined in June and October, 1998 was not increased remarkably by FSP application, but the number of colonies of bacteria determined in March, 1998 showed a significant increase with increasing level of FSP application. In conclusion, N contents and OM of soil increased with increasing level of N application, but decreased with increasing level of FSP application.

• Korean Journal of Soil Science and Fertilizer
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• v.33 no.1
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• pp.52-60
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• 2000

This study was conducted to gain basic data for alleviation of gas emission and conservation of healthy soil environment by investigating an aspect of gas emission and microbial diversity due to the supplement of different fertilizers after application with a livestock manure compost in greenhouse soils. Green pepper was cultivated in clay loamy soil from April to August. Before planting, a livestock manure compost was applied with $741mg\;ha^{-1}$ on the basis of the phosphate content contained in compost. And then, deficient nitrogen for cropping was supplemented with either quick-acting fertilizer of urea or a controlled slow release fertilizer made from urea formaldehyde(U/F). $NH_3$ and R $NH_2$ gases emitted from soil showed a low concentration in the early stage but a maximum in 27 days after planting, then decreased rapidly and not detected after 33 days. Their average concentrations were 42% and 85% lower in the treatment of slow release fertilizer than that of urea fertilizer, respectively. $CO_2$ gas emitted under urea fertilization was ranged from 1,200 to $3,200mg{\ell}^{-1}$ and that in slow release fertilizer was $900{\sim}2,650mg\;{\ell}^{-1}$. The average concentration of urea treatment was $2,260mg{\ell}^{-1}$ and 30% higher than that of slow release fertilizer. The treatment of slow release fertilizer with the lapse of cropping time populated larger in numbers of bacteria, actinomycetes, nitrate bacteria and nitrate reduction bacteria, and ratios of bacteria and actinmycetes to fungi than that of urea fertilizer. But the number of fungi was higher in the treatment of urea fertilizer and denitrifying bacteria showed a similar trend in both treatments. The microbial diversity index, which calculated with numbers of 6 species of microorganisms, was decreased with increasing of growing stage in the range of 0.1 to 0.35 and that was higher in the tratment of slow release fertilizer than urea.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.3
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• pp.173-181
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• 2013

Livestock wastes are considered as major environmental pollutants because they contain high concentration of organic materials. In 2001, The Environmental Department reported that stock farmers were increasing as 5.1%/year, which resulted in a gradual increase in livestock wastes generation. The direct disposal of livestock wastes create several environmental problems. Thus, several countries banned the disposal of livestock wastes in environment including aquatic systems. Recently, aeration-based liquid fertilization was considered as potential way for the disposal of livestock wastes. In this study, next generation sequencing (NGS) analysis was used to understand the microbial community changes during liquid fertilization of livestock wastes. Microbial community was compared with liquid fertilizer physicochemical analysis such as $BOD_5$, $COD_{Mn}$ pH, N (Nitrogen), P (Phosphorus), K (Potassium) etc. The physicochemical parameters and bacterial community results pave the way for producing effective livestock-based fertilizer. By comparing the physical characteristics of the manure with microbial community changes, it is possible to optimize the conditions for producing effective fertilizer.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.2
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• pp.146-152
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• 2015

Heavy metal pollution has been a critical problem in agricultural field near at the abandoned metal mines and chemical amendments are applied for remediation purpose. However, biological activity can be changed depending on chemical amendments affecting crop productivity. Main purpose of this research was to evaluate biological parameters after applying chemical amendments in heavy metal polluted agricultural field. Result showed that soil respiration (SR) and microbial biomass carbon (MBC) were changed after chemical amendments were applied. Among three different amendments, lime stone (LS), steel slag (SS), and acid mine drainage sludge(AMDS), AMDS had an effect to increase SR in paddy soil. Comparing to control ($93.98-170.33mg\;kg^{-1}day^{-1}$), average of 30% increased SR was observed. In terms of MBC, SS had an increased effect in paddy soil. However, no significant difference of SR and MBC was observed in upland soil after chemical amendment application. Overall, SR can be used as an indicator of heavy metal remediation in paddy soil.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.2
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• pp.122-128
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• 2013

This study was carried out to evaluate the soil dehydrogenase activity and microbial biomass C with soil type and land use in cropland of JeJu region. Soil chemical properties, dehydrogenase activity, and microbial biomass C were analyzed after sampling from upland (50 sites), orchard (50 sites), paddy (30 sites), horticultural facility (30 sites) in March. Average pH values was at 6.3 in upland soil, however soil chemical properties showed a large spatial variations in both orchard and horticultural facility soil. The Zn and Cu contents increased by the continuous application of pig manure compost in some citrus orchard soil. Soil dehydrogenase activity and microbial biomass C were higher in non-volcanic ash than in volcanic ash soil regardless of land use type. Soil dehydrogenase activity was two to four times higher in upland than in the others. It was at 38.7 ug TPF $24^{h-1}g^{-1}$ in non-volcanic ash of upland soil. Microbial biomass C content was very high in horticultural facility soil and it showed at 216.8 $mg\;kg^{-1}$ in non-volcanic ash. Soil dehydrogenase activity showed a positive correlation with organic matter ($r^2$=0.59), Zn ($r^2$=0.65), and Cu ($r^2$=0.66) in non-volcanic ash horticultural facility soil. There was a negative correlation ($r^2$=0.57) between soil organic matter and dehydrogenase activity in volcanic ash upland soil.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.1
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• pp.15-21
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• 2015

The use of cover crops has a beneficial effect on sustainable soil management in pear orchards. We aimed to compare changes in soil chemical properties and biota with the use of different cover crops. We tested the effects of five cover plants, including hairy vetch, orchard grass, rattail fescue, rye, and perennial ryegrass. Use of different cover crops had a minimal impact on soil chemical properties through three year experiments. The aboveground biomass was greatest with the use of rye. The potential amounts of returnable N and P were highest when leguminous hairy vetch was used as a cover plant. Changes in the composition of the microbial community were investigated by phospholipid fatty acid (PLFA) analysis. Microbial PLFAs were highest with the use of rattail fescue and lowest with the use of hairy vetch. Minimal changes in the abundances of nematodes and microarthropods suggested that there was no bottom-up control in the soil ecosystem. The results also show that increases in aboveground biomass and nutrient content with the use of cover crops may not promote the abundance of soil organisms.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.6
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• pp.995-1001
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• 2010

Biogeography is the study of the distribution of biodiversity over space and time. We report the development of a prototype database that maps of microbial diversity in the context of the geochemical and geological environment and geographic location. It aims to reveal where organisms live, and at what abundance in nation wide. Microbial data collected from agricultural land during 1999 to 2007 were categorized for mapping with ArcGIS program. Distribution maps of bacteria, fungi, Bacillus and gram negative bacteria of agricultural land showed different patterns from each other. Microbial biomass content investigated in year of 2007 was higher than in 1999.

• Korean Journal of Organic Agriculture
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• v.20 no.1
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• pp.81-89
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• 2012

This study was investigated to evaluate the effects of fertilizer application of surface and broadcast for rice culture on the soil chemical, physical, and microbial properties as well as growth and yield of rice. The application was made with 'Dongjin 1' rice at Jeollanam-do Agricultural Research & Extension Services from 2008 to 2010. Soil organic matter and cation concentrations were increased by surface and broadcast applications, respectively. Plots treated by surface application tended to be higher seasonal N-mineralization rate in the organic fertilizer and seasonal soil organic matter than those of broadcast application. Soil physical properties seemed to be improved by the broadcast application, and soil microbial properties were increased by the surface application. Surface application increased 5% of rice yield compared to that of broadcast.

• Korean Journal of Soil Science and Fertilizer
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• v.30 no.2
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• pp.200-207
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• 1997

In order to evaluate the soil microbiological characteristics of paddy fields in Korea, surface soils were sampled from 63 sites in different agroclimatic zones before submersion of the fields. The distribution of microorganisms and the microbial diversity indices were examined. Soil microbial populations were generally higher in southern area than in northern area. The colony forming units(cfus) of fluorescence Pseudomonas sp. showed the greatest regional differences, among the microbes investigated. On the topographical differences, the cfus of aerobic bacteria, fluorescence Pseudomonas sp. and Azotobacter sp. maintained high level in coastal plains; and on the sail textural difference, fungus was the highest in clay soil, but Bacillus sp., Azotobacter sp and denitrifiers were the highest in silty clay loam soil at 0.05 probability level based on the multiple range test. The numbers of ammonium oxidizers and Azotobacter sp. were increased with soil pH. Microbial diversity indices of paddy fields which calculated from the percentages of Bacillus sp. fluorescence Pseudomonas sp. Azotobacter sp. denitrifiers, ammonium oxidizers, nitrite oxidizers, actinomycetes and fungus to these total microbial numbers were between 0.109 and 0.661. On the soil textures, the microbial diversity indices of sandy, sandy loam, silty clay loam, clay loam and clay soil were 0.443, 0.427, 0.414, 0.405 and 0.362 respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.6
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• pp.1169-1175
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• 2011

The soil chemical properties, microbial community structures and biochemical properties of lettuce or cucumber-cultivated greenhouse soil samples were analyzed to assess soil health and characterize microbial distribution in 8 locations in Korea. Although most of chemical properties were within the soil management guidelines, the available phosphate, and the contents of exchangeable potassium and calcium were higher than those of recommended levels. In the culture-dependent analysis, 841 bacterial strains were isolated from the greenhouse soils and were identified at the genus level by 16S rRNA gene sequences analysis. The dominant bacterial genera were Bacillus (35.7%), Microbacterium (9.3%), Arthrobacter (5.7%) and Lysobacter (5.1%). The abundance of pseudomonads was highly variable depending on the soil samples. In the culture-independent analysis, soil microbial community was investigated by using phospholipid fatty acid (PLFA) method. Principal component analysis (PCA) showed that a specific grouping for microbial community structure in the greenhouse soils was not observed based on cultivated crops and investigated sites. The results revealed that the greenhouses soils examined are relatively sound managed in terms of soil chemical contents and microbial properties.