Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Characterization of Microbial Community Changes in Process Affected by Physicochemical Parameters During Liquid Fertilization of Swine Waste

  • Shin, Mi-Na (Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Kim, Jin-Won (Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Shim, Jaehong (Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Koo, Heung-Hoe (Semyong Engineering) ;
  • Lee, Jai-Young (Department of Environmental Engineering, The University of Seoul) ;
  • Cho, Min (Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Oh, Byung-Taek (Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University)
  • Received : 2013.05.06
  • Accepted : 2013.05.22
  • Published : 2013.06.30
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Abstract

Livestock wastes are considered as major environmental pollutants because they contain high concentration of organic materials. In 2001, The Environmental Department reported that stock farmers were increasing as 5.1%/year, which resulted in a gradual increase in livestock wastes generation. The direct disposal of livestock wastes create several environmental problems. Thus, several countries banned the disposal of livestock wastes in environment including aquatic systems. Recently, aeration-based liquid fertilization was considered as potential way for the disposal of livestock wastes. In this study, next generation sequencing (NGS) analysis was used to understand the microbial community changes during liquid fertilization of livestock wastes. Microbial community was compared with liquid fertilizer physicochemical analysis such as $BOD_5$, $COD_{Mn}$ pH, N (Nitrogen), P (Phosphorus), K (Potassium) etc. The physicochemical parameters and bacterial community results pave the way for producing effective livestock-based fertilizer. By comparing the physical characteristics of the manure with microbial community changes, it is possible to optimize the conditions for producing effective fertilizer.

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References

  1. Adams, I.P., R.H. Glover, W.A. Monger, R. Mumford, E. Jackeviciene, M. Navalinskiene, M. Samuitiene, and N. Boonham. 2009. Next-generation sequencing and metagenomic analysis: a universal diagnostic tool in plant virology. Mol. Plant Pathol. 10:537-545. https://doi.org/10.1111/j.1364-3703.2009.00545.x
  2. Beaudet, R., C. Gagnon, J. Bisaillon, and M. Ishaque. 1990. Microbiological aspects of aerobic thermophilic treatment of swine waste. Appl. Environ. Microbial. 56(4):971-976.
  3. Dong, X. and G.B. Reddy. 2010. Soil bacterial communities in constructed wetlands treated with swine wastewater using PCR-DGGE technique. Bioresour. technol. 101:1175-1182. https://doi.org/10.1016/j.biortech.2009.09.071
  4. Gajewska, E. and M. Sklodowska. 2009. Nickel-induced changes in nitrogen metabolism in wheat shoots. J. Plant Physiol. 166:1034-1044. https://doi.org/10.1016/j.jplph.2008.12.004
  5. Gutierrez, J., C.O. Hong, B.H. Lee, and P.J. Kim. 2010. Effect of steel-making slag as a soil amendment on arsenic uptake by radish (Raphanus sativa L.) in an upland soil. Biol. Fertil. Soils. 46:617-623. https://doi.org/10.1007/s00374-010-0470-z
  6. Gangwar, S. and V.P. Singh. 2011. Indole acetic acid differently changes growth and nitrogen metabolism in Pisum sativum L. seedlings under chromium (VI) phytotoxicity: Implication of oxidative stress. Sci. Hortic. 129:321-328. https://doi.org/10.1016/j.scienta.2011.03.026
  7. Hess, T.F., I. Grdzelishvili, H. Sheng, and C.J. Hovde. 2004. Heat inactivation of E. coli during manure composting. Compost sci. Utilization. 12: 314-322. https://doi.org/10.1080/1065657X.2004.10702200
  8. Holzel, C., K. Harms, H. Kuchenhoff, A. Kunz, C. Muller, K. Meyer, K. Schwaiger, and J. Bauer. 2010. Phenotypic and genotypic bacterial antimicrobial resistance in liquid pig manure is variously associated with contents of tetracyclines and sulfonamides. Appl. Microbiol. 108:1642-1656. https://doi.org/10.1111/j.1365-2672.2009.04570.x
  9. Jin, S.N., H.J. Lee, and S.D. Oh. 2003. Growth, leaf nitrogen contents, and nitrate reductase activity in pear (Pyrus pyrifolia cv. Niitaka) trees as affected by ammonium and nitrate nitrogen supply. J. Kor. Soc. Hort. Sci. 44(1):82-86.
  10. Jung, K.S., S.G. Heu, E.J. Roh, D.H. Lee, J.C. Yun, and K.H. Kim. 2011. Prevalence of pathogenic bacteria in livestock manure compost and organic fertilizer. Korean J. Soil Sci. Fert. 44(5):824-829. https://doi.org/10.7745/KJSSF.2011.44.5.824
  11. Jeong, K.H., M.A. Khan, C.H. Kim, D.H. Lee, D.Y. Choi, and Y.H. Yu. 2012. Effect of aeration mechanism on livestock manure liquid fertilization. J. Organic Agri. 20(4):703-713. https://doi.org/10.11625/KJOA.2012.20.4.703
  12. Kim, D.I. and D.H. Kim, 2006, Bacterial community structure and diversity of the Zoysia japonica soil treated with liquid fertilizer containing amino acids. Korean J. Microbiol. 42(2):103-110.
  13. Kim, K.S. 2007. The present conditions and measures for ocean dumping of livestock extra wastes in korea. J. Of The Korean Society Of Marine Environmental & Safety. 13(4):27-35.
  14. Kim, T.S., H.S. Kim, S.D. Kwon, and H.D. Park, 2010. Analysis of bacterial community composition in wastewater treatment bioreactors using 16S rRNA gene-based pyrosequencing. Korean J. Microbiol. 46(4):352-358.
  15. Kwon, Y.R., J. Kim, B.K. Ahn, S.B. Lee, 2010. Effect of Liquid Pig Manure and Synthetic Fertilizer on Rice Growth, Yield, and Quality. Korea J. of Environmental Agriculture. 29(1): 54-60. https://doi.org/10.5338/KJEA.2010.29.1.054
  16. Kim, B.C., S. Kim, T. Shin, H. Kim, and B.I. Sang. 2013. Comparison of the Bacterial Communities in Anaerobic, Anoxic, and Oxic Chambers of a Pilot A2O Process Using Pyrosequencing Analysis. Curr. Microbiol. 66(6):555-565. https://doi.org/10.1007/s00284-013-0311-z
  17. Nam, Y.D., S.Y. Lee, and S.I. Lim. 2012. Microbial community analysis of Korean soybean pastes by next-generation sequencing. Int. J. Food Microbiol. 155(1-2):36-42. https://doi.org/10.1016/j.ijfoodmicro.2012.01.013
  18. Park, H.S., J.H. Sul, and M.H. Chiang, 1998. Effects of ammonium and nitrate on callus growth of tobacco and soybean and activities of nitrogen metabolizing enzymes. Korean J. Plant Tissue Culture. 25(1):57-61.
  19. Park, E.J., J. Chun, C.J. Cha, W.S. Park, C.O. Jeon, and J.W. Bae. 2011. Bacterial community analysis during fermentation of ten representative kinds of kimchi with barcoded pyrosequencing. Food microbiol. 30(1):197-204.
  20. Reysenbach A.L., L.J. Giver, G.S., Wickham and N.R., Pace. 1992. Differential amplification of rRNA genes by polymerase chain reaction. Appl Environ Microbiol. 58(10):3417-3418.
  21. Ryoo, J.W., 2010. Effects of mixture application of concentrated pig slurry and byproduct liquid fertilizer on the growth and yield of chinese cabbage. Korean J. of Organic Agriculture. 18(2):271-282.
  22. Sung, G.M., D.G. Lee, and S.J. Park. 2011. The bacterial community structure in biofilms of the RABC process for swine butchery wastewater treatment. Korean J. Microbiol. 47(1):56-65.
  23. Song, J.Y., B.K. Kim, S.K. Kwon, M.J. Kwak, and J.F. Kim. 2012. Next-generation sequencing for environmental biologyfull-fledged environmental genomics around the corner. Korean J. Environ. Biol. 30(2):77-89.
  24. Unno, T., J. Jang, D. Han, J.H. Kim, M.J. Sadowsky, O.S. Kim, J. Chun, and H.G. Hur. 2010. Use of barcoded pyrosequencing and shared OTUs to determine sources of fecal bacteria in watersheds. Environ. Sci. Technol. 44:7777-7782. https://doi.org/10.1021/es101500z
  25. Wagner M., A. Loy, R. Nogueira, U. Purkhold, N. Lee, H. Daims. 2002. Microbial community composition and function in wastewater treatment plants. Antonie Leeuwenhoek Int. J. Gen. M. 81:665-680. https://doi.org/10.1023/A:1020586312170