• Korean Journal of Soil Science and Fertilizer
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• v.45 no.4
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• pp.515-523
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• 2012

The study investigated the biochemical methane potential (BMP) assay of cellulose supplementing with mixed methanogens and cellulolytic bacteria to improve anaerobic digestion for methane production. For the BMP assay, 7 different microbial supplementation groups were consisted of the cultures of mixed methanogens (M), Fibrobacter succinogenes (FS), Ruminococcus flavefaciensn (RF), R. albus (RA), RA+FS and M+RA+FS including control. The cultures were added in the batch reactors with the increasing dose levels of 1% (0.5 mL), 3% (1.5 mL) and 5% (2.5 mL). Incubation for the BMP assay was carried out for 40 days at $38^{\circ}C$ and anaerobic digestate obtained from an anaerobic digester with pig slurry as inoculum was used. In results, 5% FS increased total biogas and methane production up to 10.4~22.7% and 17.4~27.5%, respectively, compared to other groups (p<0.05). Total solid (TS) digestion efficiency showed a similar trend to the total biogas and methane productions. Generally the TS digestion efficiency of the FS group was higher than that of other groups showing at the highest value of 64.2% in the 5% FS group. Volatile solid (VS) digestion efficiencies of 68.4 and 71.0% in the 5% FS and the 5% RF were higher than other groups. After incubation, pH values in all treatment groups were over 6.4 indicating that methanogensis was not inhibited during the incubation. In conclusion, the results indicated that the hydrolysis stage for methane production in anaerobic batch reactors was the late-limiting stage compared with the methanogenesis stage, and especially, as the supplementation levels of F. succinogenes supplementation increased, the methane production was increased in the BMP assay compared with other microbial culture addition.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.3
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• pp.400-407
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• 2011

To evaluate the aquatic ecological characteristics in Sinpyongcheon constructed wetlands for treating nonpoint source pollution, the removal rates of nutrients in water, the total amounts of T-N and T-P uptakes by water plants, and chemical characteristics of T-N and T-P in sediment were investigated. The concentrations of BOD, COD, SS, T-N and T-P in inflow were 0.07~1.47, 0.60~2.65, 0.50~4.60, 1.38~6.26 and $0.08{\sim}0.32mg\;L^{-1}$, respectively. The removal rates of BOD, COD, SS, T-N, and T-P were 14%, 6%, 18%, 24%, and 10%, respectively. The maximum amount of T-N uptake by water plants in August was $813mg\;plant^{-1}$ for Phragmites communis TRIV in $2^{nd}$ bed, $1,172mg\;plant^{-1}$ for Typha orientalis PRESL in $3^{rd}$ bed, respectively. The maximum amount of T-P uptake by water plants in August was $247mg\;plant^{-1}$ for Phragmites communis TRIV in $2^{nd}$ bed, $359mg\;plant^{-1}$ for Typha orientalis PRESL in $3^{rd}$ bed, respectively. Organic matter, T-N, and T-P contents in sediments were high in the order of $1^{st}$ bed > $2^{nd}$ bed > $3^{rd}$ bed. Microbial biomass C/N/P ratios in sediments in $1^{st}$, $2^{nd}$, and $3^{rd}$ were 78~110/3~6/1, 73~204/1~6/1, and 106~169/1~6/1, respectively.

• Korean Journal of Organic Agriculture
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• v.10 no.3
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• pp.67-77
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• 2002

This study was conducted to investigate an effects on ago-chemical alternative materials such as the wood vinegar, a lactic acid bacteria serum, the fermented plant juice, the brown rice vinegar and a Chitosan used for amount and qualities of fruits and to examine the pest protection efficiency for their uses in the apple and pear orchard farms. An apple yields in the orchard cultivated with using the ago-chemical alternative materials without appling the fertilizer and pesticides were decreased at 56% relative to the conventional farming practice method. Also, it was indicated that there was difficult to produce the fruits with marketability because the small sizes of fruits were produced. For the quality of fruits, the brix of apple produced in the orchard cultivated with using the ago-chemical alternative materials was similar, but Vitamin C content was greater than that of the conventional farming practice method. As a results of treating with the wood vinegar, a lactic acid bacteria serum, the fermented plant juice, the brown rice vinegar and a Chitosan instead of applying pesticides, the fruit disease in the Chitosan treatment was a little decreased, but was great occurred in the other treatments compared with the conventional farming practice mehod. However, it observed that brix and Vitamin C content of apple produced in the Chitosan, brown rice vinegar, fermented plan juice and fish amino acid treatments and in the Chitosan, brown rice vinegar, charcoal power and peat moss treatments were greater than those of the conventional farming practice method, respectively. Over all, it considered that there was very difficult to manage the orchard depended on the ago-chemical alternative materials without appling the chemical fertilizer and pesticides in the apple orchard, but it might be proper to use the ago-chemical alternative materials as an auxiliary means to decrease the appling amount of chemical fertilizer and pesticides. Furthermore, the general effects on the ago-chemical alternative materials to the perennial fruits should be investigated with considering the changes of soil fertility, soil microbial status and natural enemy creatures after treating them for a long time.

• Journal of Korea Soil Environment Society
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• v.4 no.3
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• pp.45-56
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• 1999

A biofilter was established to remove the ammonia, which is representative nitrogen-contained malodorous gas. in a compost factory. Removal efficiency of ammonia and hydrogen sulfide also was investigated. A quantity of malodor gas produced in a compost factory was affected greatly by the weather. compost states and working condition of a fertilizing mixer, and the produced gas concentrations doubled by above various parameters. By operating a water scrubbing system for removing water-soluble malodorous gases effectively. we could improve the removal efficiency over three times. We investigated long-term stability of biofilter under continuous gas flow(SV=500h-1) for 100 days. The results showed 30 days of microbial retention time. After the days, deodorization efficiency of biofilter was kept steady state. and the removal efficiency was kept over 95% for ammonia and 97% for hydrogen so]fide. respectively. The electric consumption of the biofilter, which could treat malodorous gas of 100$\textrm{m}^3$/min, applied in the compost factory was evaluated about 80u0day and water consumption was 80~100$\ell$/day. These results concluded that the biofilter is a excellent deodorization technology as well as cost-effective for removing malodorous gas produced in a compost factory.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.2
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• pp.200-208
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• 2010

The objectives of this study were to evaluate no-tillage cover crop cropping systems for organic farming in paddy. The experiment was performed at Ihyeon series (silt loam: 9.1% sand, 73.0% silt and 17.9% clay) which affected by different management practices. Planthopper population per 20 plant was significantly higher 65.3 for conventional tillage with chemical compounds (control) compared with 3.4~9.6 for no-tillage treatments (without rice straw or green manure, amended with rice straw, hairy vetch, rape, rye, and Chinese milk vetch). Also, disease severity of sheath blight was significantly higher 10.5% for control compared to 0.7~2.9% for no-tillage treatments. Four weed species, namely Monochoria vaginalis, Ludwigia prostrata, Rotala indica, and Aneilema keisak occurred in no-tillage paddy, whereas Monochoria vaginalis occurred in control only. The pH, available phosphate, and microbial biomass C in paddy were steeply decreased in response to submerging, but increased at first heading stage. Soil $NH_4$-N content at first heading stage was significantly higher in no-tillage treatments compared with control. The grain yield was significantly higher in 4.30 Mg $ha^{-1}$ for control than other treatments. Meanwhile, rice productivity was significantly higher in 2.69 Mg $ha^{-1}$ for no-tillage amended with Chinese milk vetch compared to other no-tillage treatments. The number of panicle per plant, grain number perpanicle, and percent ripened grain were highly related for increasing the yield of rice. These results show that Chinese milk vetch was optimum cover crop for organic farming in no-tillage paddy.

• Korean Journal of Soil Science and Fertilizer
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• v.28 no.2
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• pp.191-205
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• 1995

The carrier materials used for the development of bacterial inoculants to be effective in field were made with various carrier materials of two major forms, alginate bead and powder inoculants. Inoculants were prepared after mixing those carrier materials with Pseudomonas fluorescens SSL3 and Bacillus subtilis B5, and the treatment effects of each inoculants was investigated on cucumber, tomato, pepper and potato. Survival density of SSL3 and B5 in various carrier materials for duration of storage and the bead inoculants were better than the powder. In the powders, survival rate increased in carrier materials treated 5% skimilk. The growth condition of microorganisms in carrier materials is good at powder. When they were preserved in the long period, contamination is problem. Scanning(200 to 600nm) of the P. fluorescens SSL3 supernatant in centrifuged MKB broth incubated for 48h had two main peaks, pyochelin(300nm) and pyoverdin(400nm). The potato yield in field experiments of spring, treated with bead formulas showed increase of 22~29% in whole potato breeds as compared with control, because the bead formulas degraded, and released the antibiotic microorganisms in slow and constant rate. In the pot experiment, there were significant difference in soil, wheatbran, and bead formed wheatbran.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.6
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• pp.447-453
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• 2007

With a broad objective for the development of microbial based fertilizers, a total of 373 strains were isolated from rhizoplane and rhizosphere of pepper, tomato, lettuce, pasture, and grass. The efficacy of the isolates to augument overall plant growth was evaluated. After screening for their plant growth promotion and antagonistic properties in vitro efficient strains were further selected. The most efficient strains was characterized by 16S rRNA gene sequences and biochemical techniques and was designated as Bacillus subtilis S37-2. The strains facilitated plant growth and inhibited the plant phathogenic fungi such as Fusarium oxysporum (KACC 40037, Rhizoctonia solani (KACC 40140), and Sclerotinia sclerotiorum (KACC 40457). Pot based bioassay using lettuce as test plant was conducted by inoculating suspension ($10^5$ to $10^8cells\;mL^{-1}$) of B. subtilis S37-2 to the rhizosphere of lettuce cultivated in soil pots. Compared with non-inoculated pots, marked increase in leaf (42.3%) and root mass (48.7%) was observed in the inoculation group where the 50ml of cell mixture ($8.7{\times}10^8cells\;ml^{-1}$) was applied to the rhizosphere of letuce either once or twice. Antagonistic effects of B. subtilis S37-2 strain on S. sclerotiorum (KACC 40457) were tested. All the tested lettuce plants perished after 9 days in treatment containing only S. sclerotiorum, but only 17% of lettuce was perished in the inoculation plot. B. subtilis grew well in the TSB culture medium. The isolates grew better in yeast extracts than peptone and tryptone as nitrogen source. The growth rate was 2~4 times greater at $37^{\circ}C$ as compared with $30^{\circ}C$ incubation temperature. B. subitlis S37-2 produced $0.1{\mu}g\;ml^{-1}$ of IAA (indole 3-acetic acid) in the TSB medium containing L-tryptophan($20mg\;L^{-1}$) in 24 hours.

• Korean Journal of Soil Science and Fertilizer
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• v.23 no.1
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• pp.21-25
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• 1990

The amounts of monosaccharides in acid hydrolysates of decomposing plant residues under laboratory conditions were determined. Straw of cereal rice and barley, wild grass cutting, and litters of deciduous and coniferous forest trees were treated to decompose for 90 days. Samples for the analysis of mono-saccharides were taken at 3 different periods of incubation. 1. Fractions of monosaccahrides in plant residues steadily decreased with the time of decomposition. In some samples there appeared an intermediate stages where the fractions reached the highest level. 2. Decomposition of barley straw occured at a faster rate than that of rice straw, and so did the decomposition of deciduous litter than that of coniferous litter. 3. Cereal crop residues of rice and barley were richer in monosaccharides than residues of wild grass cutting and forest litters. 4. Distiction between monosaccharides of plant origin and those of microbial origin was not possible to make in this study. 5. Glucose was the predominent monosaccharide and fucose was the monosaccharide contained in the smallest amount. No measurable ribose was detected from any sample. 6. The relative proportion of galactose in creased with the time of incubation. 7. In general, the proportion of fucose decreased with time and so did that of rhamnose, rhamnose of rice straw residue being the exception. 8. The orders of abundance of monosaccharides after decomposition of 90 days were as the following: in rice straw; glucose > xylose > arabinose > galactose > rhamnose > mannose > fucose, in barley straw; glucose > xylose > arabinose > galactose> mannose > rhamnose > fucose, in wild grass cutting; glucose > xylose > galactose arabinose> rhamnose mannose > fucose, in deciduous litter; glucose > arabinose > xylose galactose > mannose > rhamnose = fucose, and in coniferous litter; glucose > xylose > galactose > arabinose mannose > rhamnose > fucose.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.1
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• pp.8-15
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• 2003

If contaminated river water is sprayed over a floodplain, the microbial processes can simultaneously remove organic matter and nitrogen during the infiltration through the sediment profile. The effect of rhizosphere on the removal of organic matter and nitrogen from contaminated river water was investigated using floodplain lysimeters. River water was sprayed at a rate of $68.0L\;m^{-2}\;d^{-1}$ on the top of the lysimeters with or without weed vegetation on the surface, Concentrations of $NO_3$, $NH_4$ and dissolved oxygen (DO), and chemical oxygen demand (COD) and Eh in water were measured as functions of depth for 4 weeks after the system reached a steady state water flow and biological reactions. A significant reductive-condition for denitrification developed in the 30-cm surface profile of lysimeters with weeds. At a depth of 30 cm, COD and $NO_3$-N concentration decreased to 5.2 and $0.9mg\;L^{-1}$ from the respective influent concentrations of 18.2 and $9.8mg\;L^{-1}$. The removal of $NO_3$ in lysimeters with weeds was significantly higher than in those without weeds. Vegetation on the top was assumed to remove $NO_3$ directly by absorption and to create more favorable conditions for denitrification by supply of organic matter and rapid $O_2$ consumption, In the lysimeters without weeds, further removal of $NO_3$ was limited by the lack of an electron donor, i.e. organic matter. These results suggest that the filtration through native floodplains, which include rhizospheres of vegetation on the surface, can be effective for the treatment of contaminated river water.

• Journal of Korean Society of Environmental Engineers
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• v.29 no.7
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• pp.820-825
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• 2007

Earthworm casting was the natural fertilizer that contained high concentrations of nutrients such as nitrogen, phosphate and potassium and of over $10^8$ CFU/ml of microorganisms. Greater than 80% of feed was excreted through the fermentation by the intestinal enzyme, after worm had eaten feeds such as fallen leaves and rotten roots under the ground. Also, the soil structure of casting was known to be very efficient in the aspects of the porosity, the water permeability, and deodorizing activities. In this research, the biofilter packed with a biomedia made of casting and waste polyurethane foam, a binder, which helped to improve the durability and perpetuity of casting, was investigated to degrade malodorous hydrogen sulfide gas. The biomedia had no need of extra supply of nutrients and of microbial inoculations. On the beginning of the operations, it showed 100% removal of hydrogen sulfide gas without lag phase. At SV of 50 $h^{-1}$, hydrogen sulfide gas from the outlet of the biofilter was not detected, when inlet concentration increased to 450 ppmv. After that, removal efficiency decreased as increasing inlet hydrogen sulfide concentration. Hydrogen sulfide removal was maintained at almost 93% until inlet concentration was increased up to 950 ppmv, at which the elimination capacity of $H_2S$ was 61.2 g $S{\cdot}m^{-3}{\cdot}h^{-1}$. Maximum elimination capacity guaranteing 90% removal was 61.2, 65.9, 84.7, 89.4 g $S{\cdot}m^{-3}{\cdot}h^{-1}$ at SV ranging from 50 $h^{-1}$ to 300 $h^{-1}$, but was 59.3 g $S{\cdot}m^{-3}{\cdot}h^{-1}$ at SV of 400 $h^{-1}$. The results calculated from Michaelis-Menten equation revealed that $V_m$ increased from 66.04, 88.96, 117.35, 224.15, to 227.54 g $S{\cdot}m^{-3}{\cdot}h^{-1}$ with increasing space velocity in the range of 50 $h^{-1}$ to 400 $h^{-1}$. However, saturation constant$(K_s)$ decreased from 79.97 ppmv to 64.95 and 65.37 ppmv, and then increased to 127.72 and 157.43 ppmv.