• 아시안잔디학회지
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• 제5권1호
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• pp.11-22
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• 1991

This experiment was executed to investigate the possibility of the application of taxonomic method through the isoelectric focusing with polyacrylamide gel and sodium dodecyl sulfate-polyacrylamide gel electrophoresis with seeds in the identification of turfgrasses. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to investigate the pattern of seed proteins which were extracted from 18 cultivars of cool season turfgrass and 4 cultivars of warm season turfgrass. The isoelectric focusing with polyacrylarnide gel was used to investigate the activity of the three isozymes of esterase, peroxidase and phosphoglucose isomerase which were extracted from 18 cultivars of cool season turfgrass and 4 cultivars of warm season turfgrass. The results were summarized as follows. 1. The difference of the patterns of seed proteins was observed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The identification of intra-genus was easily detected. 2. The three isozymes of esterase, peroxidase and phosphoglucose isomerase were investigated through isoelectric focusing with polyacrylamide gel. As a result, esterase was most effective among three isozymes in the identification of turfgrass cultivars 3. In the past cultivar identification was primarily based on visual morphological characters, but there was a lot of difficulty. If we should use electrophoresis, we will be able to identifvturfgrass cultivars more effectively.

• Bulletin of the Korean Chemical Society
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• 제32권12호
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• pp.4253-4257
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• 2011

Sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE) and capillary isoelectric focusing (CIEF) were employed to characterize and compare ricin E purified from the small grain seeds of Ricinus communis with ricin D isoform. During the purification of ricin E using ion-exchange and size-exclusion chromatography, SDS-CGE was found to be useful for monitoring the efficiencies of purification steps. SDS-CGE showed that the molecular size of ricin E was not significantly different from that of ricin D, which was confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. CIEF was useful for discriminating ricin E from ricin D based on their isoelectric points (pI). The pI values of ricin E and D were 8.6-8.8 and 7.0-7.4, respectively. This study demonstrates the usefulness of SDS-CGE and CIEF for the characterization of ricin toxins.

• 한국진공학회지
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• 제17권2호
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• pp.170-174
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• 2008

고립된 탄소나노튜브를 얻기 위해 계면활성제 Sodium Dodecyl Sulfate(SDS) 수용액에 단층 탄소나노튜브 분말을 넣어 초음파 처리를 하는 과정 중에 발생하는 물성 변화를 라만과 Photoluminescence를 통하여 연구하였다. 단층 탄소나노튜브(SWCNT) radial breathing mode(RBM)의 라만신호 세기의 변화는 SWCNT의 chirality에 따라 서로 다른 경향성을 보이고 초음파 처리 시간에 영향을 받음을 확인하였다. 또한 동일한 농도의 계면활성제에 담긴 SWCNT의 농도가 커지면 G-band 라만 진동수가 작아지면서 Photoluminescence 세기가 증가하는 현상을 관측하였다.

• Applied Biological Chemistry
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• 제32권2호
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• pp.85-90
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• 1989

다수확계(통일벼) 및 일반계 각각 3가지 품종의 각 출수후 6시기의 쌀종자에서 2% sodium dodecyl sulfate/5% ${\beta}-mercaptoethanol$로 단백질을 추출하여 1차원에서 등전점에 따라 분리한 후 2차원에서 분자량에 따라 분리하는 2차원 전기영동으로 각각의 단백질 지도를 작성하였다. 종합단백질 지도를 작성하였고 pH $5.2{\sim}8.3$, 분자량 $20,000{\sim}100,000$의 범위에서 300개 이상의 spots이 관찰되었다. 품종 및 출수시기에 따라 단백질 지도가 많은 차이를 보였다.

• 분석과학
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• 제11권3호
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• pp.151-155
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• 1998

100 mM NaCl+27 mM $MgCl_2$ 용액에서, 도데실황산나트륨 (SDS)의 유무에 따른 1.0 mM 메틸비올로겐 ($MV^{2+}$)의 전기화학적 거동을 연구하였다. ${\Delta}E_p$(산화와 환원 봉우리전위 값의 차이) 대 - log[SDS]로 도시한 그림에서 두 선의 교차점을 임계미셀농도 (CMC)로 결정하였다. $Mg^{2+}$가 첨가되면, 유리탄소전극에 대한 효과적인 $MV{\cdot}^+$의 접근이 가능해지고 미셀형성도 지연되었다.

• 한국응용과학기술학회지
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• 제26권1호
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• pp.74-79
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• 2009

The inorganic-organic composite particles with core-shell structure were polymerized by using styrene and potassium persulfate (KPS) as a shell monomer and an initiator, respectively. We studied the effect of surfactants on the core-shell structure of silicone dioxide/styrene composite particles polymerized in the presence of sodium dodecyl sulfate(SDS), polyoxyethylene alkylether sulfate (EU-S133D), and at none surfactant condition. We found that $SiO_2$ core / polystyrene(PS) shell structure was formed when polymerization of styrene was conducted on the surface of $SiO_2$ particles, and the concentration SDS and EU-Sl33D was $8.34{\times}10^{-2}mole/L$. The core-shell structure was confirmed by measuring the thermal decomposition of the polymer composite using thermogravimetric analyzer (TGA), and the morphology of the composite particles was characterized by transmission electron microscope (TEM).

• Bulletin of the Korean Chemical Society
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• 제18권2호
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• pp.185-189
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• 1997

The effect of pH and sodium dodecyl sulfate (SDS) micelle on the quenching of Ru(bpy)32+ luminescence by N-alkyl-4,4'-bipyridinium ions (RBPY+: R=methyl, octyl, dodecyl, benzyl) were investigated. In the absence of SDS, the quenching rate at pH 2 is similar to that of the corresponding methylalkyl viologens and much greater than that in pH 8 solution due to greater reducibility of the protonated form of RBPY+ (HRBPY2+). The quenching rate at pH 2 is strongly enhanced by the presence of SDS, while that at basic pH is greatly retarded. These observations are explained by deep embedment of RBPY+ into the hydrophobic hydrocarbon region of the micelle, whereas Ru(bpy)32+ and HRBPY2+ locate in the Stern layer of the micelle.

• 대한한의학회지
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• 제27권2호
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• pp.103-110
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• 2006

Objectives : This study was conducted to investigate the effects of ginseng saponins and commercial surfactants such as Triton X-100, sodium deoxycholate, and sodium dodecyl sulfate on the gastric enzyme-catalyzed hydrolysis. Methods : Saponins (a surface-active plant component) from fresh ginseng root were extracted to examine its effect on the gastric enzyme-catalyzed hydrolysis. Commercial surfactants such as Triton X-100, sodium deoxycholate, and sodium dodecyl sulfate were also employed in the hydrolysis system to compare their effects with that of the ginseng saponins. The effects of surfactants on the gastric enzyme-catalyzed hydrolysis were measured by using a spectrophotometer. A spectropolarimeter was used to examine the conformational change of enzymes and substrates by the addition of ginseng saponins into the system. Results : Both the tryptic and the peptic digestion of milk casein or eggalbumin were slightly improved with an increase in the amount of ginseng saponins in the system. Triton X-100 showed an effect similar to that of ginseng saponins, while sodium dodecyl sulfate and sodium deoxycholate diminished the hydrolysis. Circular dichroism spectra of enzymes and substrates was significantly changed by the addition of ginseng saponins into the system. Conclusions : These results show that ginseng saponins affect positively the gastric enzyme-catalyzed hydrolysis, and suggest that the digestion of substrates by gastric enzymes is affected by the change of enzyme conformation by ginseng saponins.

• 폴리머
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• 제28권2호
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• pp.154-161
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• 2004

폴리(4-비닐피리딘)의 질소 원자에 메틸기를 부착시켜 4차아민화시켜서 부분적으로 개질된 폴리(4-비닐피리딘)을 얻었고, 부분 개질된 폴리(4-비닐피리딘)의 구조를 수용액상에서 레이저 광산란 및 형광법 등을 이용하여 조사한 결과, 자기 응집현상에 의하여 핵-껍질 구조를 가지고 있는 것으로 나타났다. 즉 사슬의 소수성 부분은 상호작용에 의하여 중심부에 모여있고, 4차화된 아민의 양전하를 가진 친수성 부분은 바깥 껍질을 형성하고 있었다. 이 고분자 수용액에 음이온 계면활성제인, 도데실 황산 소듐을 첨가하면 이들 사이에 임계 응집 농도가 존재하며, 이 임계 응집 농도는 특히 NaCl 농도 0.1 M 이상에서는 급격히 감소하는 경향을 보여주었다. 또한 계면활성제의 첨가에 따라 생성된 고분자-계면활성제의 복합 응집체의 입자크기 변화 등을 동적 광산란으로 측정 분석하였다.

• 한국임상수의학회지
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• 제24권3호
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• pp.305-307
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• 2007

락토페린 결합단백질(Lactoferrin-binding proteins, LBP)은 젖소유방염 원인균인 Streptococcus uberis의 막단백질로서 그 특성에 관해서는 잘 규명되어 있지 않지만, 특히 최근에는 스트렙토코커스성 유방염의 독성인자로서 중요시되고 있다. 본 연구에서는 S. uberis 네 가지 균주를 대상으로 LBP를 보다 효율적으로 추출하기 위하여 mutanolysin 및 sodium dodecyl sulfate(SDS)를 이용한 두 가지 다른 추출 방법을 사용하였다. 추출된 세균단백질을 SDS-polyacrylamide gel electrophoreis(SDS-PAGE)로 전기영동을 하였고, 겔을 니트로셀룰로스 막으로 이동시켰다. Rabbit anti-bovine lactoferrin 항체와 HRP-conjugated donkey anti-rabbit IgG 항체를 사용하여 LBP를 검출하였다. 이러한 웨스턴 블롯팅 분석을 통해 SDS 추출법이 mutanolysin 추출법에 비해 보다 효율적으로 110 kDa 및 112 kDa의 LBP를 추출할 수 있음을 증명하였다.