• 생명과학회지
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• 제20권5호
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• pp.649-654
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• 2010

Streptococcus equi subsp. zooepidemicus는 말의 생식기 질환을 유발하는 중요한 병원체 중 하나로서 국내 말의 생산성 향상을 도모할 목적으로 제주도에서 사육중인 더러브렛 암말의 유산태아 및 그 말의 자궁내용물과 장수목장에서 암말의 교배전 생식기 질환이 의심되는 말의 질 내용물로부터 시료를 채취하여 Streptococcus equi subsp. zooepidemicus을 분리하여 그 분리균의 특성 및 항균제 감수성 검사를 실시한 결과, RAPD typing에서는 크게 4개의 cluster로 구분되었다. 특히 제주도에서 분리한 균주와 장수목장에서 분리한 하나의 균주가 동일한 pattern을 나타내었다. 항균제 감수성 검사에서는 대부분의 균주가 ampicillin 등의 약제에 감수성이 있음을 확인하였다. 본 연구의 결과는 국내에서 사육중인 암말의 생식기 질병의 예방 및 치료에 유용할 것으로 생각된다.

• Weed & Turfgrass Science
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• 제5권1호
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• pp.5-9
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• 2016

본 연구는 2015년 4월부터 6월(1차 조사), 9월부터 10월까지(2차 조사) 2차례에 걸쳐 대전광역시와 세종특별자치시를 포함한 충청남도지역의 사과원에 발생하는 잡초분포현황과 잡초관리방법을 확인하기 위하여 수행되었다. 조사결과 충남지역 사과원에 27과 64종의 잡초가 발생하는 것을 확인하였다. 이 중 일년생잡초는 39종이였으며 다년생 잡초는 25종이였다. 1차 조사에서는 새포아풀(6.51), 개불알풀(5.36), 질경이(5.36)순으로 우점하였으며, 2차 조사에서는 별꽃(5.73), 바랭이(5.36), 소리쟁이(5.18)순으로 우점 하였다. 발생잡초를 과별로 분류하면 국화과가 12종으로 가장 많이 발생하였고 화본과는 7종, 마디풀과는 6종 발생하였다. 잡초관리 방법에 대한 설문조사 결과 충남지역 사과원의 55%가 예초와 제초제나 경운 또는 초생재배를 함께 사용하는 것으로 조사되었다. 예초를 통해 잡초관리를 할 경우 25종의 잡초가 발생하여 방제효과가 낮은 것으로 조사되었다. 충남지역 사과원에서 선호하는 제초제는 glufosinate-ammonium으로 연간 2-3회 사용하는 것으로 조사되었다.

• The Korean Journal of Ecology
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• 제25권4호
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• pp.253-259
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• 2002

Application of phytoremediation in the polluted area to remove undesirable materials is a complex and difficult subject without detailed investigation and experimentation. We investigated the accumulation patterns of cadmium and lead in plants naturally grown, the bioavailability of plants to accumulate these toxic metals and the responses of P. thunbergii to cadmium and lead. The soil samples contained detectable lead (＜$17.5_\mu$g/g), whereas cadmium was not detected in the soils of study area. The whole body of Persicaria thunbergii contained detectable lead (＜320.$8_\mu$g/g/g) but cadmium was detected only in the stem (＜7.$4_\mu$g/g/g) and root (＜10.$4_\mu$g/g/g) of P. thunbergii. Cadmium was not detected in Trapa japonica and Nymphoides peltata, whereas lead was detected in T. japonica (＜323.$7_\mu$g/g/g) and N. peltata (＜177.$5_\mu$g/g/g). Correlation coefficient between lead content in soil and in these plant samples represented positive correlation. The total content of lead in each plant sample increased in the order of N. peltata$\leq$P. thunbergii

• 한국식품영양과학회지
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• 제28권3호
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• pp.654-662
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• 1999

The purpose of this study was to investigate the effects of vitamin E on the antioxidative defense system of kidney in streptozotocin induced diabetic rats. Sprague Dawley male rats weighing 100$\pm$10g were randomly assigned to one normal and three STZ induced diabetic groups, which were subdivided into vitamin E free diet(DM 0E group), 40mg vitamin E per kg diet(DM 40E group) and 400mg vitamin E per kg diet(DM 400E group). Vitamin E level of normal group was 40 mg per kg diet. Diabetes was experimentally induced by intravenous injection of 55mg/kg of body weight of streptozotocin(STZ) in citrate buffer(pH 4.3) after 4 weeks feeding of experimental diets. Animals were sacrificed at the 6th day of diabetic states. There were no significant on body weights, food intakes, and food efficiency ratio before the diabetic occurrence. But after the injection of STZ, body weights and food efficiency ratios were significantly decreased and the food intakes was increased. Kidney weights were significantly increased in diabetic groups compared to normal group. However, there were no significant differences among the diabetic groups. Plasma insulin levels of diabetic groups were significantly decreased, whereas, blood sugar levels were increased compared to that of normal group. There were no significant differences among diabetic groups in plasma insulin and glucose levels. Activities of superoxide dismutase(SOD) in DM 0E and DM 40E groups were signi ficantly decreased by 33% and 27%, respectively, compared to normal group. But that of DM 400E group was increased by 35% compared to DM 0E group. Activity of glutathione peroxidase(GSHpx) in DM 0E group was decreased by 20% compared with normal group. GSHpx activity in DM 400E group was increased by 29% compared to normal group. The contents of vitamin E in kidney were 58% and 49% lower in DM 0E and DM 40E group, respectively, than normal group. There was no significant difference in renal vitamin E contents between DM-400E group and normal group. The contents of superoxide radical(O2 ) in kidney were 150% and 98%, respectively, higher in DM 0E and DM 40E groups than normalgroup. DM 400E and normal groups were similar levels in their superoxide radical contents of kidneys. These results indicate that vitamin E functioned as chain breaking antioxidant in kidney such as in other tissues.

• 한국식품영양과학회지
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• 제42권5호
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• pp.721-729
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• 2013

본 연구는 염농도의 저하에 따른 저염 오징어젓갈의 유통기한 감소에 대하여 유통기한의 연장과 기능성 제품화의 가능성을 검토하기 위하여 천연식물추출물 첨가에 따른 저장기간별 품질특성, 최적발효기한, 유통기한 설정 및 생리활성을 연구하였으며, 그 결과는 다음과 같다. 첨가군 모두 저장온도가 증가할수록 pH는 급격히 감소하였고, 아미노질소, 휘발성염기질소의 함량은 급격히 증가하였으며, 총균수 역시 증가하였다. 유통기한 및 최적발효기한은 Arrhenius equation의 결과에 따라 $10^{\circ}C$에서 대조구 34 및 28일, 월계수첨가 35 및 29일, 녹차첨가 34 및 30일, 솔잎첨가 34 및 29일 저장한 것이었다. 구성 및 유리아미노산은 첨가군 모두가 glutamic acid, glycine, alanine 등의 감칠맛, 단맛을 내는 아미노산의 함량이 대부분을 차지하여 맛에서 우수하다고 판단되었고, 핵산관련물질은 첨가군 모두 쓴맛을 나타내는 hypoxanthine이 대부분을 차지하였으며, 그 뒤 inosine 및 정미성분인 IMP 순이었다. 관능검사결과 월계수첨가군이 종합적으로 가장 뛰어났다. 생리활성은 첨가군이 대조구에 비해 활성이 높았으며, 기능성 제품으로서의 상업화가 가능하다고 판단된다. 그러므로 천연식물추출물을 첨가한 저염 오징어젓갈은 웰빙 등 시대의 변화에 맞추어 현대인의 식기호에 적합한 식품으로 이용 가능하며, 젓갈의 고급화 및 부가가치 창출이 가능하다고 사료된다.

• Applied Microscopy
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• 제37권1호
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• pp.1-10
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• 2007

MCT 유발 간독성 흰쥐에 해독정기탕의 투여가 시간경과에 따른 간세포의 항산화효소 활성변화, 세포사멸 억제와 간의 조직병리학적 변화에 미치는 영향을 조사하였다. 대조군은 생리식염수를 투여하였으며, 실험군은 해독정기탕 0.9 mL을 6시간, 24시간 간격으로 경구투여 하였다. 간기능의 지표가 되는 GPT 효소 활성은 해독정기탕 투여한 6시간 군에서 대조군에 비해 감소하였다. 항산화 효소인 SOD, catalase 활성도의 변화에 있어서도 6시간 실험군에서 변화를 보여주었고 24시간 경과 후에는 대조군과 실험군의 통계적인 유의성은 없었다. 간의 조직병리학적 관찰 결과 대조군은 심한 출혈, 간세포의 팽창과 중심정맥 내피세포의 파괴 현상이 뚜렷이 나타났으며, 24시간 경과 후에는 핵질의 응축과 사립체의 팽대현상이 현저하였다. 반면 해독정기탕 투여후 6시간 실험군은 일부 세포괴사와 세포사멸 보호 및 회복효과를 관찰하였다. 이상의 연구 결과로 보아 해독정기탕은 투여시간에 따라 간 기능회복 및 해독, 간세포의 항산화 활성변화, 세포사멸의 보호효과 약물로서 작용함이 사료된다.

• 한국약용작물학회지
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• 제18권3호
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• pp.157-167
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• 2010

We investigated a method to improve anticancer activities of Acer mono wood extracts by ultra high pressure extraction process. The A. mono was extracted by water at $40^{\circ}C$ and 300 MPa for 15 min (High Pressure Extraction, HPE). The extraction yield by ultra high pressure extraction process was 5.42%. The cytotoxicity on human normal lung cell (HEL299) of the extracts from HPE showed 21.54% lower than that from conventional water extraction at $100^{\circ}C$ in adding the maximum concentration of 1.0 mg/$m{\ell}$. Ultra high pressure extracts process for 15 minutes extracts (HPE15) showed more potent scavenging effect than the control, BHA. On SOD-like test, the HPE15 showed highest activity as 32.4% at 1.0 mg/$m{\ell}$ concentration. Human stomach adenocarcinoma, liver adenocarcinoma, breast adenocarcinoma and lung adenocarcinoma cell growth were inhibited up to about 67~79%, in adding 1.0 mg/$m{\ell}$ of extracts from HPE. HPE was 20~25% higher than conventional water extraction. It was interesting that, among several cancer cell lines (stomach adenocarcinoma, liver adenocarcinoma), the growth of digestive related cancer cells were most effectively inhibited as about 75~79%. On in vivo experiment using ICR mice, the variation of body weight of mice group treated A. mono wood extracts from HPE of 100 mg/kg/day concentration was very lower than control and other group. The survival times of group treated this extracts was 61.96% longer than that of the control group and this extracts showed the lower tumor weight, which were 10.49 g than positive control as 16.17 g. Based on these results, we could tell that the HPE wood extracts of A. mono had higher anticancer activity than conventional water extraction. The results of HPE showed obvious advantages in higher efficiency, shorter extraction time, at lower energy costs.

• 한국약용작물학회지
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• 제18권5호
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• pp.323-328
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• 2010

Root extract of Lythrum salicaria reported a hepato-protective effect on $CCl_4$-induced liver toxicity of rat was prepared into fractions such as n-hexane up layer (HA), n-hexane down layer (HB), diethyl ether (E), ethylacetate (EA), n-butanol (B) and water (W). Fractions prepared were tested their activities in vitro and in vivo condition. All of the fractions showed effective antioxidant asctivities on DPPH radical and $CuSO_4$-induced oxidation of human low density lipoprotein and E fraction showed the highest inhibitory effect (98.1% at $50\;{\mu}g/m{\ell}$) on linoleic acid autoxidation at $40^{\circ}C$, which was more effective than $\alpha$-tocopherol (82.4%). Five fractions (H = HA plus HB, E, EA, B, and W, 150 mg/kg/day) were fed into Sprague Dawley, male rats for 4 days, which were intoxicated with intra-peritoneal injection of carbon tetrachloride ($1\;m{\ell}/kg$ in corn oil) at the 4th day and were sacrificed in 24 hrs. Serum tumor necrosis factor-alpha (TNF-$\alpha$), a proinflammatory cytokine, elevated with $CCl_4$-intoxication in negative control group ($83\;pg/m{\ell}$) was significantly decreased in E fraction-supplemented group ($18\;pg/m{\ell}$). Cu, Zn-superoxide dismutase (SOD) activity increased in negative control group (0.12 U/mg protein) was decreased in E fraction (0.07 U/mg protein). From the results, it is suggested that ether fraction from root extract of L. salicaria would be a potent antioxidant candidate for ameliorating liver injury induced by chemical intoxicant.

• Weed & Turfgrass Science
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• 제5권3호
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• pp.165-169
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• 2016

잔디 뗏장의 효율적인 생산을 위한 토양 관리 시스템을 구축하기 위해 토양관리에 따른 잔디의 생육에 대한 연구를 수행하였다. 토양관리는 4가지 방법 (1) 답압 처리구 (2) 배토 처리구 (3) 배토 +답압 처리구 (4) 배토 +경운 +답압 혼합 처리구로 실시하였다. 토양의 물리성은 배토 +경운 +답압 혼합 처리구가 답압과 배토 +답압 처리구에 비해 용적비중과 공극률, 고상비 등이 통계적으로 유의하게 개선되었으나, 배토 처리구와는 통계적으로 유의한 차이가 없었다. 토양 관리방법에 따른 잔디생육의 특성을 조사한 결과, 답압작업에 비해 배토만 하거나 배토와 함께 토양 경운의 방법을 추가하였을 때 식물체의 생육이 향상되는 결과를 나타내었다. 따라서 잔디 연작 재배지의 경우 토양 물리성 개선을 위한 배토, 경운, 답압등의 토양 갱신작업을 통해 잔디의 적정밀도 유지와 양질의 잔디를 생산할 수 있도록 해야 할 것이다.

• Korean Journal of Acupuncture
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• 제27권1호
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• pp.49-62
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• 2010

Objective: This study was performed to investigate the effect of manual acupuncture, invasive laser acupuncture and laser skin irradiation at LU8, LR4, HT8 and LR2(Liver Seunggyeok) on D-galN-induced liver injury in rats. Method: Liver injury was induced with D-galN. The experimental rats were divided four groups(Control group, EXP-1, EXP-2, EXP-3). In the Control group, liver injury-induced and not treated. EXP-1 group was liver injury-induced and carried out manual acupuncture with Young-Su(against the meridian course and following the course of the meridian) & Won-Bang(by twisting and rotating the needle) acupuncture method at Liver Seunggyeok. EXP-2 group was liver injury-induced and carried out invasive laser acupuncture at Liver Seunggyeok. EXP-3 group was liver injury-induced and carried out laser skin irradiation at Liver Seunggyeok. Result: In the change of body weight(in 1 week), EXP-1, EXP-2 and EXP-3 groups were significantly increased as compared with control group. In the change AST & ALT, EXP-1 and EXP-2 groups were significantly decreased as compared with control group. In the change of SOD, EXP-1, EXP-2 and EXP-3 groups were significantly increased as compared with control group. In the change of WBC, EXP-2 group was significantly increased as compared with control group. Conclusion: Manual acupuncture, invasive laser acupuncture and laser skin irradiation at Liver Seunggyeok had hepatotherapeutic effect on the treatment of hepatocytotoxity. Invasive laser acupuncture was as effective as manual acupuncture on the treatment of hepatocytotocity.