Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Biological Activity of Organic Solvent Fractions from Lythrum salicaria L. (Root)

털부처꽃 뿌리로부터 조제된 분획물의 생리활성

  • 이승은 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김금숙 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 한희선 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이은숙 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영옥 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이정훈 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 성낙술 ((재)금산국제인삼약초연구소) ;
  • 이상원 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영철 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • Received : 2010.08.29
  • Accepted : 2010.10.11
  • Published : 2010.10.30
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Abstract

Root extract of Lythrum salicaria reported a hepato-protective effect on $CCl_4$-induced liver toxicity of rat was prepared into fractions such as n-hexane up layer (HA), n-hexane down layer (HB), diethyl ether (E), ethylacetate (EA), n-butanol (B) and water (W). Fractions prepared were tested their activities in vitro and in vivo condition. All of the fractions showed effective antioxidant asctivities on DPPH radical and $CuSO_4$-induced oxidation of human low density lipoprotein and E fraction showed the highest inhibitory effect (98.1% at $50\;{\mu}g/m{\ell}$) on linoleic acid autoxidation at $40^{\circ}C$, which was more effective than $\alpha$-tocopherol (82.4%). Five fractions (H = HA plus HB, E, EA, B, and W, 150 mg/kg/day) were fed into Sprague Dawley, male rats for 4 days, which were intoxicated with intra-peritoneal injection of carbon tetrachloride ($1\;m{\ell}/kg$ in corn oil) at the 4th day and were sacrificed in 24 hrs. Serum tumor necrosis factor-alpha (TNF-$\alpha$), a proinflammatory cytokine, elevated with $CCl_4$-intoxication in negative control group ($83\;pg/m{\ell}$) was significantly decreased in E fraction-supplemented group ($18\;pg/m{\ell}$). Cu, Zn-superoxide dismutase (SOD) activity increased in negative control group (0.12 U/mg protein) was decreased in E fraction (0.07 U/mg protein). From the results, it is suggested that ether fraction from root extract of L. salicaria would be a potent antioxidant candidate for ameliorating liver injury induced by chemical intoxicant.

Keywords

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