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Biological Activity of Organic Solvent Fractions from Lythrum salicaria L. (Root)  

Lee, Seung-Eun (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Geum-Soog (Department of Herbal Crop Research, NIHHS, RDA)
Han, Hee-Sun (Department of Herbal Crop Research, NIHHS, RDA)
Lee, Eun-Suk (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Young-Ok (Department of Herbal Crop Research, NIHHS, RDA)
Lee, Jeong-Hoon (Department of Herbal Crop Research, NIHHS, RDA)
Seong, Nak-Sul (International Ginseng & Herb Research Institute)
Lee, Sang-Won (International Ginseng & Herb Research Institute)
Kim, Young-Chul (International Ginseng & Herb Research Institute)
Publication Information
Korean Journal of Medicinal Crop Science / v.18, no.5, 2010 , pp. 323-328 More about this Journal
Abstract
Root extract of Lythrum salicaria reported a hepato-protective effect on $CCl_4$-induced liver toxicity of rat was prepared into fractions such as n-hexane up layer (HA), n-hexane down layer (HB), diethyl ether (E), ethylacetate (EA), n-butanol (B) and water (W). Fractions prepared were tested their activities in vitro and in vivo condition. All of the fractions showed effective antioxidant asctivities on DPPH radical and $CuSO_4$-induced oxidation of human low density lipoprotein and E fraction showed the highest inhibitory effect (98.1% at $50\;{\mu}g/m{\ell}$) on linoleic acid autoxidation at $40^{\circ}C$, which was more effective than $\alpha$-tocopherol (82.4%). Five fractions (H = HA plus HB, E, EA, B, and W, 150 mg/kg/day) were fed into Sprague Dawley, male rats for 4 days, which were intoxicated with intra-peritoneal injection of carbon tetrachloride ($1\;m{\ell}/kg$ in corn oil) at the 4th day and were sacrificed in 24 hrs. Serum tumor necrosis factor-alpha (TNF-$\alpha$), a proinflammatory cytokine, elevated with $CCl_4$-intoxication in negative control group ($83\;pg/m{\ell}$) was significantly decreased in E fraction-supplemented group ($18\;pg/m{\ell}$). Cu, Zn-superoxide dismutase (SOD) activity increased in negative control group (0.12 U/mg protein) was decreased in E fraction (0.07 U/mg protein). From the results, it is suggested that ether fraction from root extract of L. salicaria would be a potent antioxidant candidate for ameliorating liver injury induced by chemical intoxicant.
Keywords
Lythrum salicaria; Antioxidant Activity; DPPH; LDL;
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