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http://dx.doi.org/10.5352/JLS.2010.20.5.649

RAPD Analysis and Antimicrobial Susceptibility of Streptococcus equi subsp. zooepidemicus Isolated from Thoroughbred Horses  

Choi, Seong-Kyoon (College of Veterinary Medicine, Kyungpook National University)
Park, Yong-Soo (Gyeongsangbukdo Livestock Research Institute)
Cho, Kwang-Hyun (Gyeongsangbukdo Livestock Research Institute)
Cho, Gil-Jae (College of Veterinary Medicine, Kyungpook National University)
Publication Information
Journal of Life Science / v.20, no.5, 2010 , pp. 649-654 More about this Journal
Abstract
A total of 68 samples were collected including vaginal mucosa (n=66) from Jangsu stud farm, an equine aborted fetus (n=1), and uterine contents (n=1) from Jeju island. Seventeen Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) strains isolated from horses in Korea were identified as S. zooepidemicus by biochemical tests and sodA.seeI specific multiplex PCR. All isolated strains were divided into 4 clusters: group 1 (No. 2, 3, 5, 6, 7, 11, 12, 13, 14, 15), group 2 (No. 4, 9), group 3 (No. 10, 16, 17), and group 4 (No. 1, 8) by RAPD typing. In group 3, No. 10 isolate that was isolated from vaginal mucosa was indistinguishable from No. 16 and 17 isolates, which were isolated from the equine uterine contents and the equine aborted fetus, respectively. The results of this study suggest that a limited epidemiological relationship exists between the strains from Jangsu (No. 10) and Jeju (No. 16 and No. 17). All isolates showed a high susceptibility to ampicillin, cefoxitin, ceftiofur, cephalothin, florofenicol, gentamicin, nalidixic acid, oxacillin, penicillin, tiamulin, tylosin and vancomycin in antimicrobial susceptibility tests. These results may provide the basic information needed to establish strategies for the treatment and prevention of reproductive diseases in mares in Korea.
Keywords
Antimicrobial susceptibility; equine; Streptococcus zooepidemicus;
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