• Molecules and Cells
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• v.39 no.10
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• pp.728-733
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• 2016

Mesenchymal stem cells (MSCs) effectively reduce airway inflammation and regenerate the alveolus in cigarette- and elastase-induced chronic obstructive pulmonary disease (COPD) animal models. The effects of stem cells are thought to be paracrine and immune-modulatory because very few stem cells remain in the lung one day after their systemic injection, which has been demonstrated previously. In this report, we analyzed the gene expression profiles to compare mouse lungs with chronic exposure to cigarette smoke with non-exposed lungs. Gene expression profiling was also conducted in a mouse lung tissue with chronic exposure to cigarette smoke following the systemic injection of human cord blood-derived mesenchymal stem cells (hCB-MSCs). Globally, 834 genes were differentially expressed after systemic injection of hCB-MSCs. Seven and 21 genes, respectively, were up-and downregulated on days 1, 4, and 14 after HCB-MSC injection. The Hbb and Hba, genes with oxygen transport and antioxidant functions, were increased on days 1 and 14. A serine protease inhibitor was also increased at a similar time point after injection of hCB-MSCs. Gene Ontology analysis indicated that the levels of genes related to immune responses, metabolic processes, and blood vessel development were altered, indicating host responses after hCB-MSC injection. These gene expression changes suggest that MSCs induce a regeneration mechanism against COPD induced by cigarette smoke. These analyses provide basic data for understanding the regeneration mechanisms promoted by hCB-MSCs in cigarette smoke-induced COPD.

• Journal of Environmental Health Sciences
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• v.40 no.1
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• pp.10-16
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• 2014

Objectives: This pilot study assessed secondhand smoke (SHS) exposure in smoking and non-smoking nightclubs in Seoul, Korea by measuring the concentration of particulate matter smaller than $2.5{\mu}m$ ($PM_{2.5}$). Methods: This comparative study was conducted in three nightclubs in Seoul. While one non-smoking nightclub was measured on weekdays and weekends, different smoking nightclubs were measured on weekdays and weekends. The concentration of $PM_{2.5}$ was observed using a real-time monitor over an average of three hours. The number of people in the clubs was also estimated. Settled dust was collected in a smoking and a non-smoking nightclub and analyzed for NNK concentration. Results: The $PM_{2.5}$ concentration in the smoking nightclubs was higher than those found in the non-smoking nightclub by 26 times on weekdays and three times on weekends. Indoor $PM_{2.5}$ concentration was correlated with the number of people in the smoking nightclubs. Relatively high $PM_{2.5}$ concentration was observed in the non-smoking nightclub on weekends. NNK concentration in the smoking nightclub was 7 times higer than in the non-smoking nightclub. Conclusion: Smoking in nightclubs caused high $PM_{2.5}$ concentration. Although the non-smoking nightclub had a lower $PM_{2.5}$ concentration, $PM_{2.5}$ concentration on weekends was higher due to the smoking room. Complete prohibition of smoking in nightclubs can protect patrons from secondhand smoke exposure.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.2
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• pp.621-626
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• 2015

Background: Smokefree laws aim to protect employees and the public from the dangers of secondhand smoke. Waterpipe premises have significantly increased in number in the last decade, with anecdotal reports of poor compliance with the smokefree law. The literature is bereft of information pertaining to waterpipe premise employees. This study aimed to opportunistically gather knowledge about the occupational health hazards associated with working in waterpipe premises in London, England. Materials and Methods: Employees from seven convenience-sampled, smokefree-compliant waterpipe premises in London were observed for occupational activities. Opportunistic carbon monoxide (CO) measurements were made among those with whom a rapport had developed. Observations were thematically coded and analysed. Results: Occupational hazards mainly included environmental smoke exposure. Waterpipe-serving employees were required to draw several puffs soon after igniting the coals, thereby providing quality assurance of the product. Median CO levels were 27.5ppm (range 21-55ppm) among these employees. Self-reported employee health was poor, with some suggestion that working patterns and smoke exposure was a contributory factor. Conclusions: The smokefree law in England does not appear to protect waterpipe premise employees from high levels of CO. Continued concerns surrounding chronic smoke exposure may contribute to poor self-reported physical and mental wellbeing.

• Korean Journal of Oriental Medicine
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• v.3 no.1
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• pp.261-278
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• 1997

This experiment was performed in order to study the clinical effects of Gyumsuyukgunjun on the pulmonary injury caused by exposure of cigarette smoke in rats. Therefore, a writer reports on present experimental results having significant effects on the level of oxygen consumption, the lungs volume, the leukocyte, the hemoglobin level, and the $PaO_2$, $PaCO_2$ after administration of Gyumsuyukgunjun in order to study the clinical effects of Gyumsuyukgunjun on the pulmonary injury caused by exposure of cigarette smoke in rats. The results were obtained as follows: 1. In comparison with control group, The group of Gyumsuyukgunjun administration was revealed significant effects(P<0.005) of increase on the level of oxygen consumption. 2. In comparison with control group, The group of Gyumsuyukgunjun administration was revealed significant effects(P<0.005) of decrease on the lungs volume. 3. In comparison with control group, The group of Gyumsuyukgunjun administration was revealed significant effects(P<0.05) of decrease on the leukocyte counts. 4. In comparison with control group, The group of Gyumsuyukgunjun administration was revealed significant effects(P<0.05) of decrease on the hemoglobin level. 5. In comparison with control group, The group of Gyumsuyukgunjun administration was revealed significant effects(P<0.01) of increase on the $PaO_2$ level only of $PaO_2$, $PaCO_2$ level. It was concluded from the above results that Gyumsuyukgunjun has a significant effects on the pulmonary injury caused by cigarette smoke.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.26 no.4
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• pp.411-417
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• 2016

Objectives: The aims of this study were to determine the indoor level of environmental tobacco smoke (ETS) and to assess the implementation rate of smoke-free laws at hospitality venues around a university campus by measuring particulate matter smaller than $2.5{\mu}m$ ($PM_{2.5}$) as an indicator of ETS. Materials and Methods: We measured indoor $PM_{2.5}$ concentrations at 20 PC game rooms, 20 pubs, and 20 billiards halls using Sidepak AM510, a direct reading portable real time monitor, from October to December 2015. Results: Smoking was observed in 65% of the PC game rooms, 10% of pubs, and 85% of billiards halls. The average $PM_{2.5}$ concentrations were $98.2{\mu}g/m^3$, $29.0{\mu}g/m^3$, and $134.2{\mu}g/m^3$ at PC game rooms, pubs, and billiards halls, respectively. $PM_{2.5}$ concentrations in PC game rooms and billiards halls were 2 to 2.7 times higher than the 24-hour exposure standard for outdoor $PM_{2.5}$ ($50{\mu}g/m^3$) by the Ministry of Environment. Conclusions: Although a smoking ban has been implemented for PC rooms and pubs, smoking is still taking place in many of these places. More stringent legal action is required for successfully protecting patrons and workers from secondhand smoke exposure. A ban on smoking in billiards halls should be introduced as quickly as possible.

• Journal of Environmental Health Sciences
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• v.44 no.3
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• pp.267-274
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• 2018

Objectives: This study examined the impact of a smoke-free policy on indoor air quality at indoor recreation facilities by assessing $PM_{2.5}$ concentrations before and after the implementation of the new policy. Methods: Using real-time monitors, $PM_{2.5}$ concentrations were measured in 50 billiard rooms and 50 golf simulator rooms in Seoul, Korea. The characteristics of the indoor recreation facilities, smoking status, and atmospheric conditions were recorded at the same time.After the enforcement of a smoke-free policy, $PM_{2.5}$ concentrations, installation of smoking room, and smoking status were examined when the facilities were revisited. Results: Almost a half of the billiard rooms and over 80% of golf simulator rooms were located underground. Seventy percent of the billiard rooms and one hundred percent of the golf simulator rooms were equipped with a local exhaust ventilation system. After the implementation of the smoke-free policy, 46% of the billiard rooms and 20% of the golf simulator rooms newly installed a smoking room. In the billiard rooms with a newly-installed smoking room, the $PM_{2.5}$ concentrations decreased from 97.9 to $45.6{\mu}g/m^3$ after the implementation of the smoke-free policy. The same change of 29.0 to $ 26.3{\mu}g/m^3$ was not statistically significant in golf simulator rooms. Indoor $PM_{2.5}$ concentrations were correlated with outdoor $PM_{2.5}$ concentrations, number of smokers, and number of people in the room. Conclusions: The smoke-free policy for indoor recreation facilities was not effective at making the indoor spaces free from second hand smoke. Although a few billiard rooms installed a smoking room, indoor $PM_{2.5}$ concentrations were still higher than those of outdoor $PM_{2.5}$ or atmospheric $PM_{2.5}$. Stricter enforcement of the smoke-free policy should be achieved to prevent secondhand smoke exposure.

• Journal of Environmental Health Sciences
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• v.41 no.1
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• pp.11-16
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• 2015

Objectives: This study assessed the impact of the smoke-free law on secondhand smoke exposure in computer game rooms by measuring concentrations of particulate matter smaller than $2.5{\mu}m$($PM_{2.5}$). Methods: Indoor $PM_{2.5}$ concentrations were measured in 56 (during the smoke-free guidance period) and 118 computer game rooms (after the law went into effect) in four cities (Seoul, Cheonan, Daegu and Kunsan) in Korea. The number of smokers in the computer game rooms was also counted every five minutes. Results: Although a smoking ban had been implemented nationally, smoking was observed in 47% of the computer game rooms. Smoking density decreased from 1.62 persons per $100m^3$ during the guidance period to 0.32 persons per $100m^3$ after the smoke-free law. There is no statistically significant difference of $PM_{2.5}$ concentrations before and after the smoking ban. The $PM_{2.5}$ concentration was two times higher than the US NAAQS of $35{\mu}g/m^3$. The $PM_{2.5}$ concentration in computer game rooms without smokers was two times higher than the outdoor concentration. Conclusion: The smoke-free law in computer game rooms was complied with, even after the guidance period, in Korea. Indoor $PM_{2.5}$ concentration after smoke-free law implementation was high due to this non-compliance. High $PM_{2.5}$ concentration in computer game rooms without smokers might be due to smoking booths. The complete prohibition of smoking in computer game rooms should be implemented to protect patrons from secondhand smoke exposure.

• Proceedings of the Korean Environmental Health Society Conference
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• 2005.11a
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• pp.35-50
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• 2005

• Journal of the Korean Society of Hazard Mitigation
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• v.10 no.5
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• pp.1-7
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• 2010

Since subway fire disaster at Daegu, Korea smoke control system and passengers evacuation distance has been focused to reform. Existing smoke control facilities need to expand volume of ventilation capacity however, the complicate subway station structure can hardly react dispersion of smokes from massive subway cabin fire. Smoke flow at platform level move upward thought vertical stairway and passengers evacuation goes with same direction. The victims of evacuees from subway station fire mainly due to exposure of heat radiation and smoke. The study demonstration the effect of downward evacuates stairway system by separating evacuation route to smoke movement pass way including saving times of evacuation.

• Journal of Environmental Health Sciences
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• v.35 no.2
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• pp.100-115
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• 2009

Secondhand smoke (SHS) is one of major public health threats. Since secondhand smoke is complex mixture of toxic chemicals, there has been no standardized method to measure SHS quantitatively. The purpose of this manuscript was to review various quantitative methods to measure SHS. There are two different methods: air monitoring and biological monitoring. Air monitoring methods include exhaled carbon monoxide level, ambient fine particulates, nicotine and 3-ethenylpyridine. Measurement of fine particulates has been utilized due to presence of real-time monitor, while fine particulates can have multiple indoor sources other than SHS. Ambient nicotine and 3-EP are more specific to SHS, although there is no real-time monitor for these chemicals. Biological monitoring methods include nicotine in hair, cotinine in urine, NNK in urine and DNA adducts. Nicotine in hair can provide chronic internal dose, while cotinine in urine can provide acute dose. Since biological monitoring can provide total internal dose, identification of specific exposure source may be difficult. NNK in urine can indicate carcinogenicity of the SHS exposure. DNA adducts can provide overall cancer causing exposure, but not specific to SHS. While there are many quantitative methods to measure SHS, selection of appropriate method should be based on purposes of assessment. Application of accurate and appropriate exposure assessment method is important for understanding health effects and establishing appropriate control measures.