• Title/Summary/Keyword: Small Samples

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Activation of Platelet Rich Plasma by Soluble Canine Small Intestinal Submucosa Gel and Bovine Thrombin (개 소장점막하 겔과 소 트롬빈을 이용한 혈소판풍부혈장의 활성화 연구)

  • Lee, A-Jin;Lee, Changsun;Kim, Hyun;Chung, Dai-Jung;Do, Sun Hee;Kim, Hwi-Yool
    • Journal of Veterinary Clinics
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    • v.31 no.3
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    • pp.170-174
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    • 2014
  • This study evaluated the efficacy of soluble canine small intestinal submucosa gel in comparison to bovine thrombin in activating rabbit platelet rich plasma (PRP) by detecting growth factors. PRP from rabbits was activated by using soluble canine SIS gel, bovine thrombin, or both. The surface morphology of each group of samples was examined by scanning electron microscopy. The release of transforming growth factor (TGF)-${\beta}1$ from each set of samples was measured over 7 days using enzyme-linked immunosorbent assay. The PRP-canine SIS gel group exhibited the highest total amount of released TGF-${\beta}1$. However, there were no significant differences between any groups. The use of soluble type of canine SIS gel could be an effective alternative to bovine thrombin.

Diet composition of the Korean wild boar Sus scrofa coreanus (Suidae) at Mt. Jeombongsan, Korea

  • Shin, Hyung-Min;Kim, Jihee;Jin, Seon Deok;Won, Ho-Yeon;Park, Sangkyu
    • Journal of Ecology and Environment
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    • v.44 no.3
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    • pp.143-154
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    • 2020
  • Background: Korean wild boars (Sus scrofa coreanus Heude), because of their adaptability, are a widespread large mammal; however, they sometimes cause problems by invading farms and eating the crops, creating insufficiencies of some foods in South Korea. To understand the diet composition of Korean wild boars according to sex and body size, we collected their feces from Mt. Jeombongsan, Seoraksan National Park, South Korea. The sizes of fecal samples were measured, and genomic DNA was extracted from the samples. We amplified specific loci targeting plants (rbcL and trnL) and animals (COI) to detect the food sources of this omnivore and amplified the ZF and SRY regions to determine the sex. Results: In the wild boar feces, Rosaceae and Bryophyte were the most frequently detected plant food sources at the family level and Diptera and Haplotaxida were the most frequently detected animal food sources at the order level. As a result of sex determination, the sex ratio of wild boars collected in the Mt. Jeombongsan area was approximately 1:1. Our result suggested that there is no significant difference between the diet composition of male and female boars. Based on the average cross-sectional area of the feces, the top 25% were classified into the large body size group and the bottom 25% were classified into the small body size group. The large body size group mainly preferred Actinidiaceae, and the small body size group most frequently consumed Fagaceae. The diet of the large body size group was more diverse than the small body size group. Conclusions: Our results showed that the wild boars preferred Rosaceae, especially Sanguisorba and Filipendula, as plant food sources, and Diptera and Coleoptera of Insecta as animal food sources. Based on the results, the dietary preferences of wild boar appear to be distinguished by not their sex but their body size. Our study could help to elucidate the feeding ecology and population structure of wild boar, as well as address conservation and management issues.

Preliminary Evaluation of the Activity Concentration Limits for Consumer Goods Containing NORM

  • Jang, Mee;Chung, Kun Ho;Ji, Young Yong;Lim, Jong Myung;Kang, Mun Ja;Choi, Guen Sik
    • Journal of Radiation Protection and Research
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    • v.41 no.2
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    • pp.101-104
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    • 2016
  • Background: To protect the public from natural radioactive materials, the 'Act on safety control of radioactive rays around living environment" was established in Korea. There is an annual effective dose limit of 1 mSv for products, but the activity concentration limit for products is not established yet. Materials and Methods: To suggest the activity concentration limits for consumer goods containing NORM, in this research, we assumed the "small room model" surrounding the ICRP reference phantom to simulate the consumer goods in contact with the human bodies. Using the Monte Carlo code MCNPX, we evaluate the effective dose rate for the ICRP reference phantom in a small room with dimension of phantom size and derived the activity concentration limit for consumer goods. Results and Discussion: The consumer goods have about 1600, 1200 and $19000Bq{\cdot}kg^{-1}$ for $^{226}Ra$, $^{232}Th$ and $^{40}K$, and the activity concentration limits are about six times comparing with the values of building materials. We applied the index to real samples, though we did not consider radioactivity of $^{40}K$, indexes of the some samples are more than 6. However, this index concept using small room model is very conservative, for the consumer goods over than index 6, it is necessary to reevaluate the absorbed dose considering real usage scenario and material characteristics. Conclusion: In this research, we derived activity concentration limits for consumer goods in contact with bodies and the results can be used as preliminary screening tool for consumer goods as index concept.

Correlation Between Expression of Cell Adhesion Molecules CD44 v6 and E-cadherin and Lymphatic Metastasis in Non-small Cell Lung Cancer

  • Su, Chong-Yu;Li, Yun-Song;Han, Yi;Zhou, Shi-Jie;Liu, Zhi-Dong
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.5
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    • pp.2221-2224
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    • 2014
  • Objective: To explore the relationship between expressions of cell adhesion molecules CD44 v6 and E-cadherin (E-cad) and lymphatic metastasis in non-small cell lung cancer (NSCLC). Materials and Methods: Eightyseven tissue samples obtained from patients with primary NSCLC were collected in our hospital from Dec., 2007 to Dec., 2012, and the expressions of CD44 v6 and E-cad gene proteins in these samples were detected by immunohistochemical method. Results: In the tissue without lymphatic metastasis, the positive expression rate of CD44 v6 was significantly lower, whereas the normal expression rate of E-cad was notably higher than that with lymphatic metastasis (55.6% vs. 78.4%, 47.2% vs. 21.6%), and both differences had statistical significance (P<0.05). Besides, CD44 v6 and E-cad expressions had a significant correlation in the NSCLC tissue with lymphatic metastasis (P<0.05). Conclusions: The positive expression of CD44 v6 and abnormal expression of E-cad may play a very important role in promoting lymphatic metastasis of NSCLC, with synergistic effect. Hence, detection of CD44 v6 and E-cad expressions is conductive to judging the lymphatic metastasis in NSCLC.

Treatment of Whitening of a Car TPE Component (자동차용 TPE 부품의 백화 처리)

  • Choi, Sung-Seen;Chung, Hye-Seung;Joo, Yong-Tae;Min, Byung-Kwon;Lee, Seong-Hoon
    • Elastomers and Composites
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    • v.45 no.2
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    • pp.94-99
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    • 2010
  • 340 nm UV radiation of $2500\;kJ/m^2$ irradiated a thermoplastic elastomer (TPE) specimen used for interior and exterior materials of a car. After the UV irradiation, the black sample surface was changed to gray. Materials on the aged sample were collected and analyzed using GC/MS, but significant materials were not found. Lots of small cracks were found on the aged specimen surface. Whitening phenomenon of the aged sample disappeared by brushing or searing with a fire on the aged surface. After brushing or searing with a fire, small cracks on the surface vanished. Samples before and after the aging, and the treated samples with brush or fire were analyzed using image analyzer, SEM, EDX, and ATR and the results were compared. The vanished whitening phenomenon by brushing or searing was explained with filling the small cracks by polypropylene of TPE.

Electrochemical Properties for the Corrosion of Zinc Anode with Different Particle Size and Shape in Zinc/air Batteries (입자의 크기가 다른 아연공기전지용 아연음극의 부식에 관한 전기화학적 특성 연구)

  • Yang, Won-Geun;Hong, Jung-Eui;Oh, Rye-Gyeong;Oh, Ji-Woo;Kong, Young-Min;Ryu, Kwang-Sun
    • Journal of Powder Materials
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    • v.20 no.3
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    • pp.186-190
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    • 2013
  • The electrochemical performance for the corrosion of zinc anodes according to particle size and shape as anode in Zn/air batteries was study. We prepared five samples of Zn powder with different particle size and morphology. For analysis the particle size of theme, we measured particle size analysis (PSA). As the result, sample (e) had smaller particle size with $10.334{\mu}m$ than others. For measuring the electrochemical performance of them, we measured the cyclic voltammetry and linear polarization in three electrode system (half-cell). For measuring the morphology change of them before and after cyclic voltammetry, we measured Field Emission Scanning Electron Microscope (FE-SEM). From the cyclic voltammetry, as the zinc powder had small size, we knew that it had large diffusion coefficient. From the linear polarization, as the zinc powder had small size, it was a good state with high polarization resistance as anode in Zn/air batteries. From the SEM images, the particle size had increased due to the dendrite formation after cyclic voltammetry. Therefore, the sample (e) with small size would have the best electrochemical performance between these samples.

Application of Modelling Stress-Strain Relations (Part I) -Application to Plane Strain Compression Tests- (응력-변형률 관계 정식화의 적용성(I) -평면변형률압축시험에 대한 적용성-)

  • Park, Choon-Sik
    • Journal of the Korean Geotechnical Society
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    • v.28 no.12
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    • pp.17-25
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    • 2012
  • FEM requires the stress-strain relationship equations for numerical analyses. However, most formulations for the stress-strain relationship published up to the present are not satisfactory enough to properly express all the levels from the small strain to the peak. Tatsuoka and Shibuya (1991) suggested a new single formulation applicable not only to a wide range of geo-materials from soft clay to soft rock, but also to a wide range of strain levels from $10^{-6}$ to $10^{-2}$. The plain strain compression test is carried out to seven samples of research standard sand specimens and two samples of glass beads, which have been used at world-renowned research institutes. In this study, strains of the maximum principal stress (${\sigma}_1$) and the minimum principal stress (${\sigma}_3$) were thoroughly measured from $10^{-6}$ to $10^{-2}$, and the result, applied to Tatsuoka and Shibuya's new formulation, coincided closely with the measured data of the stress-strain relationship from the small strain to the peak.

Scale Effects of the Specimen on Shear Strength of sand by Direct Shear Test (직접전단시험에 의한 모래의 전단강도에서 시편의 크기효과)

  • Kim, Joon-Seok;Kim, Ji-Hyun
    • Journal of the Society of Disaster Information
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    • v.15 no.4
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    • pp.590-596
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    • 2019
  • Purpose: An experimental study was conducted on the sand samples for the size effect of the test specimens, one of the problems of the direct shear test. Method: Jumunjin standard sand, a representative sand of Korea, was used as sand sample. The large direct shear test was performed to analyze the shear strength at 50%, 60%, 70%, and 80% relative density, and then the comparative results were compared with the test results of the small direct shear test. Result: It was analyzed that the internal friction angle of the small shear tester tended to be relatively large in the dense region. It was analyzed that the results of the large shear tester tend to be relatively large in the region of medium relative density. Conclusion: The size effect on the samples in the direct shear test on sandy soil was analyzed to be relatively small.

An investigation into the Online Sales Channels of Small Business Fashion Retailers on Portal Shopping and Fashion Shopping Malls (소상공인 패션판매업자의 온라인 판매채널 연구: 포털쇼핑몰과 패션쇼핑몰(종합물/전문몰)을 중심으로)

  • Son, Mi Young
    • Human Ecology Research
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    • v.59 no.4
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    • pp.449-463
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    • 2021
  • The aim of this study was to analyze the perceptions and entering status of small business online fashion retailers on portal shopping and fashion shopping malls. Case studies were conducted on a total of 10 research samples. The results were as follows: first, regarding the strategic factors of online fashion stores, 'price competitiveness' is important, especially in portal shopping and low-cost brands; 'product assortment' is important but not essential in all platforms; and 'differentiation' is important to continuously secure loyal customers in fashion shopping malls. Customer satisfaction leads to customer loyalty, and customer loyalty affects the sales conversion rate and brand growth of online sales channels. Factors that promoted sales activities in online sales channels were exposure, advertisements, SNS, events, special exhibitions, and events. Hindrance factors were low price competition, overheated competition, and the MD of sales channels. Second, the research samples used multiple online sales channels, including portal shopping malls and fashion shopping malls, in addition to their own malls. The selection factors were platform reputation and commission, branding, and customer inflow through exposure. Portal shopping malls were perceived as providing easy access, advertising/customer communication, exposure/search, price competitiveness, scalability, and intense competition, whereas fashion shopping malls were perceived as providing a brand image and concept, brand promotion, high commissions, difficult entry, and low profits. The factors for success in portal shopping malls were exposure/search, price competitiveness, and brand recognition, whereas the factors for success in fashion shopping malls were differentiation, brand, exposure/advertisement, product assortment, and MD.

Investigation on Harmful Trace Elements in Some Food Additives (식품첨가물중(食品添加物中) 유해미량금속(有害微量金屬) 함량(含量)에 관(關)하여)

  • Kirn, Jeung-Ok;Song, Jae-Chul;Young, Han-Chul;Ha, Yeung-Lae
    • Journal of Nutrition and Health
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    • v.12 no.3
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    • pp.25-32
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    • 1979
  • In order to investigate harmful trace elements in ford additives used in food processing the contents of Copper, Lead, Cadmium, Chromium, Manganese, Nickel and Iron are determined by Atomic Absorption Spectrophotometer. These Elements are Extracted with APDC-MIBK extract method. Samples are collected from the food Industrial companies in Korea. Samples studied as shown in Table 1 are food colors(3-samples, Flavoring Agents(7-samples), Acidifiers(4-samples) Baking Powders(4-samples), Emulsifiers(2-samples), Thickeners(2-samples), Antioxidants(2-samples), Intensifiers(2-samples), Seasonings(3-samples), modifiers (10-samples) Most of the food additives contained comparatively a little small amount of harmful trace elements. However, the contents of harmful trace elements of Sodium Carbonate, Food Yellow No.5, Food Blue No.1, Food Red No.2 and Alkalies added in noodles are significantly higher than the other food additives. The obtained results are as follows ; 1) Sodium Carbonate contained Cadmium; 16.73 ug/g and Lead ; 61.55 ug/g. 2) Food Yellow No. 5 contained Cadmium: 1.67ug/g and Lead; 23.46 ug/g. 3) Food Blue No. 1 contained Cadmium; 1. 16 ug/g and Lead; 23.46 ug/g. 4) Food Red Ho. 1 contained Cadmium; 1.91 ug/g and Lead 23.08 ug./g. 5) Alkalies added in noodles contained Cadmium; 6. 11 ug/g and Lead; 53.85 ug/g.

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