• 대한수의학회지
• /
• 제60권2호
• /
• pp.85-88
• /
• 2020

A 12-month-old domestic rabbit died suddenly without specific clinical signs. Gross examination revealed irregular yellowish hepatic nodules with pus in the submandibular muscles, lungs, uterus, and small intestines. Histopathological examination of the liver showed granulomatous inflammation with acid-fast-positive bacteria. Mycobacterium bovis SB1040 was identified by polymerase chain reaction and spoligotyping, and Pasteurella multocida was isolated from the multiple lesions. This report demonstrates the pathological features of rare bovine tuberculosis (bTB) in a domestic rabbit, the first case in the Republic of Korea. To ensure public safety, we recommend routine monitoring of rabbits to control the incidence of bTB.

• Plant Biotechnology Reports
• /
• 제3권2호
• /
• pp.111-126
• /
• 2009

MicroRNAs (miRNAs) are endogenous, non-coding, small RNA molecules consisting of 21-24 nucleotides (nts) that regulate target genes at the posttranscriptional level in plants and animals. In plants, miRNAs negatively regulate target mRNAs containing a highly complementary sequence by either mRNA cleavage or translational repression. MiRNAs are processed from single-stranded precursors containing stem-loop structures by a Dicer-like enzyme and are loaded into silencing complexes, where they act on target mRNAs. Although plant miRNAs were first reported in Arabidopsis 10 years later than animal miRNAs, numerous miRNAs have since been identified from various land plants ranging from mosses to flowering plants, and their roles in diverse aspects of plant developmental processes have been characterized. Furthermore, most of the annotated plant miRNAs are evolutionarily conserved in various plants. In particular, recent functional studies using Arabidopsis mutants have contributed a great deal of information towards establishing a framework for understanding miRNA biogenesis and functional roles. Extensive appraisal of miRNA-directed regulation during a wide array of plant development and plant responses to environmental conditions has confirmed the versatile roles of miRNAs as a key component of plant molecular biology.

• The Plant Pathology Journal
• /
• 제29권3호
• /
• pp.350-355
• /
• 2013

Plants protect themselves from diverse potential pathogens by induction of the immune systems such as systemic acquired resistance (SAR). Most bacterial plant pathogens thrive in the intercellular space (apoplast) of plant tissues and cause symptoms. The apoplastic leaf exudate (LE) is believed to contain nutrients to provide food resource for phytopathogenic bacteria to survive and to bring harmful phytocompounds to protect plants against bacterial pathogens. In this study, we employed the pepper-Xanthomonas axonopodis system to assess whether apoplastic fluid from LE in pepper affects the fitness of X. axonopodis during the induction of SAR. The LE was extracted from pepper leaves 7 days after soil drench-application of a chemical trigger, benzothiadiazole (BTH). Elicitation of plant immunity was confirmed by significant up-regulation of four genes, CaPR1, CaPR4, CaPR9, and CaCHI2, by BTH treatment. Bacterial fitness was evaluated by measuring growth rate during cultivation with LE from BTH- or water-treated leaves. LE from BTH-treatment significantly inhibited bacterial growth when compared to that from the water-treated control. The antibacterial activity of LE from BTH-treated samples was not affected by heating at $100^{\circ}C$ for 30 min. Although the antibacterial molecules were not precisely identified, the data suggest that small (less than 5 kDa), heat-stable compound(s) that are present in BTH-induced LE directly attenuate bacterial growth during the elicitation of plant immunity.

• 상하수도학회지
• /
• 제23권2호
• /
• pp.251-255
• /
• 2009

Foreign and domestic seawater desalination plant market investigation was performed to analyze the worldwide trend of seawater desalination plant market and to establish the activation plan for seawater desalination plant application. Water demand and seawater desalination related laws and regulations were investigated and analyzed for the activation plan. RO type and large scale plants are popular nowadays however there are only small plants in island region in Korea. There will be about $1 million\;m^3/day$ deficit in 2015 according to the water demand forecasting from Ministry of Environment and Ministry of Land, Transportation, and Maritime Affairs in Korea. Therefore, it is necessary to activate the domestic application of seawater desalination plant to secure stable water resources. To activate the domestic application of seawater desalination plant, first, we need to establish regulations, support system in the water service law for seawater desalination plant. Second, related Ministry should increase the support for the operation and management of seawater desalination plant and suggest the construction of seawater desalination plant for water resources security near seaside region.

• 한국축산식품학회지
• /
• 제41권1호
• /
• pp.8-15
• /
• 2021

Vegetarians have claimed and actively promoted the advantages of plant-based alternative milks as the best option for human nutrition and health, compared to the natural dairy milk. However, numerous scientific evidences and reports have demonstrated that the natural milk possesses more beneficial nutrients and bioactive components than artificially manufactured plant-derived milks. The biochemical and nutritional advantages and functionalities of natural dairy milk cannot be replaced by man-made or crafted plant-based beverage products. On the other hand, the tremendous increase in production and consumption of the plant-based alternative milks in recent years has led a serious business downturn in traditional roles and stability of the dairy industry, especially in the major dairy producing Western countries. Although plant-based milk alternatives may have some benefits on nutrition and health of certain consumers, the plant-derived alternative milks may not overshadow the true values of natural milk. Milk is not a high fat and high cholesterol food as animal meat products. Unlike plant-based alternative milks, natural milk contains many bioactive as well as antiappetizing peptides, which can reduce body weight. It has proven that taking low-fat, cultured and lactase treated milk and dairy products with other diversified nutritionally balanced diets have been shown to be healthier dietary option than plant-based milk/foods alone.

• Journal of Electrical Engineering and Technology
• /
• 제6권3호
• /
• pp.311-318
• /
• 2011

Small-scale reactors have recently attracted attention as a potential power generation source for the future. The Regional Energy Research Institute for Next Generation is currently developing a small-scale reactor called Regional Energy rX 10 MVA (REX-10). The current paper deals with a power system to be used with small-scale reactors for multi-purpose regional energy systems. This small nuclear system can supply electric and thermal energy like a co-generation system. The electrical model of the REX-10 has been developed as a part of the SCADA system. REX-10's dynamic and electromagnetic performance on the power system is analyzed. Simulations are carried out on a test system based on Ulleung Island's power system to validate REX-10 availability on a power system. RSCAD/RTDS and PSS/E software tools are used for the simulation.

• Journal of Plant Biotechnology
• /
• 제39권4호
• /
• pp.235-241
• /
• 2012

식물체에서 sumoylation 기작은 성장 및 발달에 중요한 기능을 수행할 것이다. 특히, SUMO E3 ligase는 SUMO 단백질을 목적 단백질로 전달해주는 마지막 단계의 sumoylation 기작 구성요소이며, 다양한 신호전달에 특이성을 나타내는 것으로 보고되고 있다. 본 연구에서는 벼에서 SUMO E3 ligase, SIZ1 유전자에 T-DNA가 삽입된 Ossiz1-2 돌연변이 식물체를 분석하였다. 그리고, OsSIZ1 단백질이 OsSUMO1 단백질과 상호작용함으로써 OsSIZ1이 SUMO E3 ligase의 기능을 수행할 것으로 예측하였다. Ossiz1-2 돌연변이 식물체는 형태학적으로 발달과 성장의 다양한 부분에서 미성숙상태로 유지됨이 보였다. 특히, 야생형인 동진벼와 비교하여 초장의 성장 및 등숙율에서 상당히 낮은 정도를 보여 주었다. 이와 같이, 벼에서 SUMO E3 ligase로써 OsSIZ1 단백질의 생리학적인 기능은 성장과 발달 그리고, 수확량에 관여하는 단백질을 sumoylation 시키는 기작에서 역할을 수행할 것으로 사려된다.

• Journal of Plant Biotechnology
• /
• 제42권4호
• /
• pp.376-379
• /
• 2015

본 연구는 낙엽송의 배발생조직 증식 및 체세포배 유도에 영향하는 유기질소원(${\small{L}}$-Glutamine 및 casein hydrolysate, CH), 식물생장조절물질(옥신 및 사이토키니류)의 종류 및 농도의 효과를 평가하기 위해 수행되었다. 배발생조직 증식 비교에서 1,000 mg/L ${\small{L}}$-Glutamine 첨가 시 2주 혹은 4주 모두 가장 높은 조직생중량을 보였고 그 외 처리구에서는 대체로 유사하였다. 체세포배 유도에서는 0.2 mg/L IBA 첨가 시 가장 높은 426.3개를 유도하여 가장 효과적이었으나 0.2 mg/L BA 혹은 Kinetin처리구에서는 전혀 체세포배가 유도되지 않았다. IBA 농도 별 비교에서는 0.2 mg/L(303개)농도가 가장 좋았으며, 1.0 mg농도에서도 281개를 유도하여 체세포배 유도에 효과적이었으나 5.0 mg/L 첨가 시 109.3개로 가장 저조하였다.

• 한국토양비료학회지
• /
• 제44권3호
• /
• pp.425-433
• /
• 2011

자연정화공법에 의한 축산폐수처리장에서 효과적으로 축산폐수를 처리하기 위해 인공습지 축산폐수처리 시스템에 전처리방법으로 수생식물여과조와 활성슬러지조를 적용하여 최적 전처리방법을 조사하였다. 또한, 최적 전처리방법을 실제 현장 축산폐수처리장에 연계하여 시공한 후 전처리방법의 효율성과 안전성을 검토하였다. 수생식물여과조와 인공습지를 연계한 소형 축산폐수처리장치에서 최종방류수 중 COD, SS, T-N 및 T-P의 처리효율은 각각 83, 89, 63 및 87%이었다. 활성슬러지조와 자연정화공법을 연계한 소형 축산폐수처리장치에서 최종방류수 중 COD, SS, T-N 및 T-P의 처리효율은 각각 96, 95, 86 및 92%이었다. 수생식물여과조 및 활성슬러지조는 인공습지 축산폐수처리장의 전처리공법으로서 질소와 인 처리효율을 향상시킬 수 있어 고농도의 축산폐수를 안정적으로 처리하기 위해 필요할 것으로 판단된다. 최적 전처리방법을 연계한 실제 현장 축산폐수처리장에서 COD, SS, T-N 및 T-P 의 처리효율은 모든 조건에서 각각 99.8, 99.9, 99.2 및 99.9%으로 높은 처리효율을 보였다.

• Current Research on Agriculture and Life Sciences
• /
• 제17권
• /
• pp.39-43
• /
• 1999

벼에 있어서 엽록체 small heat shock protein(small HSP)의 기능을 밝히기 위하여 Agrobacterium을 이용한 형질전환법을 이용하여 벼로부터 분리한 small HSP cDNA를 도입하였다. Agrobacterium의 감염에는 벼의 미성숙 배로부터 유도한 callus를 이용하였다. 형질전환 후의 재분화율은 약 30%였다. 형질전환을 통하여 얻어진 식물체의 genomic DNA로부터 PCR 분석과 Southern blot 분석으로 엽록체 small HSP 유전자의 도입을 확인하였다. 도입 유전자의 형질전환 벼에 있어서 유전자의 발현 양상을 northern blot 분석으로 조사하였다. 그 결과 도입된 유전자는 상온에서의 발현량이 서로 다르게 나타났으며 항상적으로 발현하고 있음을 확인하였다.