• Title/Summary/Keyword: Slaughter blood

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Application of methylene blue color test for the detection of inherited susceptibility to hemolysis of Korean native cattle (한우(韓牛)의 선천성용혈감수성검사(先天性溶血感受性檢査)에 대한 methylene blue 청색소실시험법(靑色消失試驗法)의 적용(適用))

  • Cho, Jong-hoo
    • Korean Journal of Veterinary Research
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    • v.28 no.2
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    • pp.327-329
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    • 1988
  • Blood samples were obtained from Korean native cattle and dairy cattle of Holstein species in the slaughter house and methylene blue color tests were performed for the detection of the inherited susceptibitity to hemolysis. Glucose-6-phosphate dehydrogenase activities expressed as the optical density obtained by methylene blue color test were the highest as 0.54 in male Korean cattle, 0.62 in female Korean cattle and 0.72 in dairy cattle of Holstein species. Percent hemolysis, packed cell volume and plasma protein contents were measured and compaired with relation to the results of methylene blue color test and no correlation were observed in each.

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Histologic Alterations in the Liver of Black Bengal Goats Infected with Fasciola gigantica

  • Howlader, M.M.R.;Huq, M.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.1
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    • pp.114-117
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    • 1997
  • A total of 77 Black Bengal goats (67 females and 10 males) of 3.5 to 5.0 years old between 18 and 20 kg liveweights were used in this study. The whole liver with gall-bladder from respective carcass was collected at slaughter following fecal and postmortem examinations. Tissue sections of all lobes of each liver were prepared and stained with hematoxylin and eosin following a standard procedure. Results confirmed the presence of flukes surrounded by fibrous capsule in the liver. All the animals were suffering from chronic Fasciola gigantica infections. The blood vessels in most of the liver tissue were thickened due to proliferation of fibrous tissue around them. Focal infiltration of lymphocytes in the lobules, patches of focal accumulation of neutrophils and eosinophils were found in all the liver tissue. Proliferations of new bile ductules in the hepatic trinity around the bile ducts that were clogged by the flukes were also observed in most tissue sections.

Serological Study on Leptospirosis in Pigs (돼지의 Leptospira 속균에 대한 혈청항체조사 - 강원도 지역을 중으로 -)

  • 정동수;김정기;박량주;김교승
    • Korean Journal of Veterinary Service
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    • v.13 no.1
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    • pp.64-68
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    • 1990
  • This experiment was to investigate the Leptospiral antibody in the pigs with the serological test in two areas of Kangwondo from March to April. 1989. Twelve different living antigen (L. icterohaemorrhagiae, L. pomona, L. hardjo, L. australis, L. canicola, L. autumnalis, L. grippotyphosa, L. tarassovi, L. pyrogenes, L. bataviae, L. lai and L. ballum) of Leptospira interrogans was used for the serological test in the pigs. The blood sample of 280 pigs collected from the slaughter houses were detected by microscopic-agg-lutination test. The results were as follows, 1. Among the serum sample of 280 heads of the pigs, 6 heads of the swine (2.14%) were positive. 2. Among the positive sample of 6 heads, 3 heads of the swine (1.1%) showed the antibody of L. ict-erohaemorrhagiae, 2 heads of the swine (0.7%) showed the antibody of L. canicola, and 1 head of the swine (0.4%) showed the antibody of L. pomona. 3. The positive rate of Leptospira interrogans in the swine of Kangwondo appeared to be very low.

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Meat Production Characteristics of Black Bengal Goat

  • Chowdhury, S.A.;Faruque, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.6
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    • pp.848-856
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    • 2004
  • Black Bengal goat is primarily reared for meat, skin comes here as a by-product. The present trial describes the effect of age on different carcass characteristics of Black Bengal goats of either sex. A total of 61 Black Bengal goats of different age and sex groups were slaughtered. They were reared under semi-intensive management on milk alone or with concentrates (of 10.14 MJ ME and 10.48 g M/kg DM) and freshly cut Napier grass (2 MJ ME and 25 g CP/kg of fresh matter) that provides the estimated NRC (1981) requirement. The four age groups were: pre-weaned kids (0-90 day), post-weaned kids (91-180 days), growing (181-365 days) and adult (>365 days). Goats were slaughtered according to 'Halal' method by severing the major vessels of the throat by a transverse cut. Different slaughter parameters of Black Bengal goat can be best predicted from the equations as follows: live weight (kg)=0.801 (shoulder height (cm))-24.32, ($r^{2}$=0.94); carcass weight (kg)=0.364 (height at hind legs (cm))-11.54, ($r^{2}$=0.91); edible weight (kg)=0.623 (shoulder height (cm))-19.94, ($r^{2}$=0.91) and saleable weight (kg)=0.701 (shoulder height (cm))-21.99, ($r^{2}$=0.92). Live weight, carcass weight, edible weight and saleable weight of castrated goat at one-year onward ranges from 20-22, 9.4-10.5, 14-16 and 16.6-18.8 kg, respectively, which are about 80% higher than most of the reported observations on Black Bengal goat of same age and sex. Slaughter weight, warm carcass weight, edible weight and saleable weight increased curvilinearly with age of slaughter but not affected (p>0.05) by sex. However, linearity of the response curve of affect of age on mentioned parameters ends at around 9 months. Visceral fat as per cent of live weight increased curvilinearly with age and attain its maximum (about 6%) at about 500 days. However, linear part of the quadratic model ends at about 300 days when visceral fat content is about 4.8% of body weight. Blood and skin yield for one-year old male goat was 797 g and 1.61 kg, respectively. Absolute yield of blood and skin increased curvilinealry and attained maximum level at about 400 days (13.3 months). Average proportion of different carcass cut were - round 27%, rump 7%, loin 10%, ribs (6-12th) 14%, shoulder 21%, Neck 7%, chest 14%. Thigh and shoulder constituted about 48.3% of the cold carcass weight. Overall crude protein content of meat samples of different carcass cuts progressively decreased with age starting from 57 at 0-90 days to 58, 47 and 33 per cent, respectively at 91-180, 181-365 and >365 days, respectively. Overall meat fat content increased almost linearly from 11.1% during 91-180 days to 22.9 and 39.5% during 181-365 and >365days, respectively. Results from this trial suggest that both carcass yield and carcass composition changes with age; and sex have little or no effect on carcass yield and carcass composition. However, caution should be made in using second conclusion as there were few female animals slaughtered relative to the male. Optimum slaughter age for Black Bengal goat reared under semi-intensive management with adequate feeding and management would be about 9 months when their live weight, warm carcass weight, edible and saleable weight of carcass can be about 16.74, 7.28, 12.05 and 13.81 kg, respectively.

High Strength Slaughter Wastewater Treatment in a Novel Combined System of Hybrid-Rotating Biological Contactor and Biological Aerated Filter (Hybrid-RBC와 BAF의 조합공정을 이용한 고농도 도축폐수의 처리 특성)

  • Jung, Chan-Il;Ahn, Jo-Hwan;Bae, Woo-Keun;Kim, Seung-Jin
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.2
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    • pp.77-84
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    • 2011
  • This study was conducted to develop a novel combined system of a hybrid rotating biological contactor (RBC) process that was composed of an attached- and suspended- biomass reactor, followed by a settler and a biological aerated filter (BAF) column to treat a high strength slaughter wastewater. Long term influences of organic and nitrogen loading rates were investigated to see how the combined system worked in terms of the removal efficiency. A synthetic wastewater containing a pork cutlet steak source (commercially available) and swine blood was used to feed the combined system. The hybrid RBC process showed excellent removals: about 95% for soluble COD and 85% for ammonium nitrogen. However, the unsettled solids seriously deteriorated the removal efficiency of total COD (TCOD) and total nitrogen (TN) in the RBC process. A significant fraction of the TCOD and suspended solids (SS) was further removed in the BAF column although the effluent quality was still unsatisfactory, giving TCOD 300 mg/L, SS 180 mg/L and TN 59 mg/L. An addition of polyaluminium chloride into the RBC effluent improved the performance of the settler and BAF, producing an excellent quality of final effluent; TCOD 16.5 mg/L, SS 0 mg/L, TN 55.5 mg/L, TP 1.3 mg/L. Therefore, it was confirmed that the combined system of hybrid RBC and BAF could treat a high strength slaughter wastewater excellently.

Studies on biochemical and immunological properties of Aetinobacillus pleuropneumoniae Isolated from the slaughter pigs with respiratory lesions in chungnam province (충남지역 도축돈의 폐병변으로 부터 분리한 Actinob acillus pleuropneumoniae의 생물학적 및 면역학적 특성)

  • 이종훈;안신욱;정영재;장경수;전무형
    • Korean Journal of Veterinary Service
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    • v.20 no.1
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    • pp.103-125
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    • 1997
  • An epidemiologic study on pleuropneumonia in the slaughter pigs(Chonan and Asan area, Chungnam province, Korea) during the period of January 1994 through December 1995 was conducted. Isolation of A pleuropneumoniae was attempted in 425 pigs with pneumonic lesions. Biochemical properties, antimicrobial susceptibility, serotypes and pathogenicity of isolated A pleuropneumoniae were investigated. In addition, outer membrane protein(OMP) of the Isolates were extracted to determine its properties and immunogenicity in both mice and piglets The results obtained through this study were summarized as followed ; 1. Of 3, 395 slaughter pigs, pleuropneumonia was observed in 425 pigs(10.6%). A pleuropneumoniae was isolated from 22 pigs(5.2%) out of 425 pigs with pneumonic lesions. The biochemical properties of all isolates were same as those of reference A pleuropneumoniae strain. Among 22 isolates, 9, 1 and 12 isolates were serovar 2, 3 and 5, respectively. 2. The results of antimicrobial susceptibility test revealed that the isolates showed high susceptibility to ciprofloxacin and cephalothin, moderate susceptibility to amikacin, gentamicin, kanamycin and streptomycin, and low susceptibility to erythromycin, tylosin and sulfadimethoxin. 3. The isolates were varied in pathogenicity to mice. Median lethal dose of LE9402(serovar 2) and LE9511(serovar 5) were $9.2{\times}10^7$ CFU and $2.8{\times}10^7$%CFU, respectively. Specific pneumonic lesions were observed from the infected mice with clinical signs. Bacteria recovery rate was high in the lung, but low In heart blood and tracheas. 4. Serovar 2 was found to be more pathogenic than serovar 5 in guinea pig. Mortality on guinea pigs inoculated with serovar 2($5.4{\times}10^8-5.4{\times}10^6$CFU) and serovar 5($2.8{\times}10^8-2.8{\times}10^6$ CFU) was 20~40% and 40~80%, respectively. A severe hemorrhagic lesions and focal pneumonic lesions were observed from dead guinea pigs. Bacteria recovery rate was relatively higher in the lung than that of other organs. 5. In the SDS-PAGE analysis, OMP-enriched fractions of both isolates and reference strains contain common peptide bands equivalent to molecular weight of 17, 27, 42, 52 and 95Kd. In addition to common peptide bands, the bands which are specific to each isolate were also observed. The profiles of Sephadex G25 fractions showed 3 major peaks. The common peptide bands which were observed by SDS-PAGE of the crude OMPs were found in the peaks 1 and 2. 6. The OMPs extracted from serovar 2(LE9402) and serovar 5(LE9511) provided high level of protection in mice(70~80%) and pigs(100%). All animals inoculated with OMPs were seroconverted, showing micro-agglutination titer of 640 to 1280.

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Reduction of slaughter age of Hanwoo steers by early genotyping based on meat yield index

  • Jeong, Chang Dae;Islam, Mahfuzul;Kim, Jong-Joo;Cho, Yong-Il;Lee, Sang-Suk
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.5
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    • pp.770-777
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    • 2020
  • Objective: This study was conducted to determine early hereditary endowment to establish a short-term feeding program. Methods: Hanwoo steers (n = 140) were equally distributed into four groups (35/group) based on genetic meat yield index (MYI) viz. the greatest, great, low, and the lowest at Jukam Hanwoo farm, Goheung. All animals were fed in group pens (5 animals/pen) with similar feed depending on the growth stage. Rice straw was provided ad libitum, whereas concentrate was fed at 5.71 kg during the growing period (6 to 13 mo) and 9.4 kg during the fattening period (13 to 28 mo). Body weight (BW) was measured at two-month intervals, whereas carcass weight was determined at slaughtering at about 31 months of age. The Affymetrix Bovine Axiom Array 640K single nucleotide polymorphism (SNP) chip was used to determine the meat quantity-related gene in the blood. Results: After 6 months, the highest (p<0.05) BW was observed in the greatest MYI group (190.77 kg) and the lowest (p<0.05) in the lowest MYI group (173.51 kg). The great MYI group also showed significantly (p<0.05) higher BW than the lowest MYI group. After 16 and 24 months, the greatest MYI group had the highest BW gain (p<0.05) and were therefore slaughtered the earliest. Carcass weight was significantly (p<0.05) higher in the greatest and the great MYI groups followed by the low and the lowest MYI groups. Back-fat thickness in the greatest MYI group was highly correlated to carcass weight and marbling score. The SNP array analysis identified the carcass-weight related gene BTB-01280026 with an additive effect. The steers with the allele increasing carcass weight had heavier slaughter weight of about 12 kg. Conclusion: Genetic MYI is a potential tool for calf selection, which will reduce the slaughter age while simultaneously increasing carcass weight, back-fat thickness, and marbling score.

Enrichment of Pork with Omega-3 Fatty Acids by Tuna Oil Supplements: Effects on Performance as well as Sensory, Nutritional and Processing Properties of Pork

  • Jaturasitha, S.;Wudthithumkanaporn, Y.;Rurksasen, P.;Kreuzer, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.11
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    • pp.1622-1633
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    • 2002
  • The effects of tuna oil supplementation (0, 1, 2 and 3%) to pig diets on growth and carcass yield as well as meat quality were determined in 40 crossbred pigs. Animals were fattened from 30 to 90 kg of live-weight. Twenty-four hours after slaughter, following various early- and late-post mortem measurements, loin, backfat and belly were prepared from the carcasses. Bacon was produced from the belly part by curing and smoking. Neither performance (feed intake, daily gains, feed conversion efficiency) nor carcass quality (slaughter weight, dressing percentage, lean percentage, nutrient composition of the loin) were significantly affected by tuna oil supplementation. Tuna oil also had no clear effects on early- and late-post mortem meat quality traits, water-holding capacity and tenderness of the M. longissiumus dorsi (LD). Colour traits of LD and backfat, and backfat firmness were not significantly affected by tuna oil, either. However, there was a certain trend to elevated fat contents of LD (and bacon), but not of backfat, with increasing levels of tuna oil in feed. Pigs receiving elevated proportions of tuna oil expressed lower VLDL cholesterol and triglyceride concentrations in blood plasma, whereas the cholesterol content of LD, backfat and bacon did not reflect this trend. Effects of tuna oil on fatty acids in LD, backfat and bacon were often small in extent, except those concerning the long-chain polyunsaturated fatty acids. With 3% tuna oil in the diet, the contents of the particularly desired omega-3 fatty acids, C20:5 and C22:6, were 0.1 and 0.2 g/kg in LD. The corresponding values for backfat and bacon were 2.6 and 12.6 g/kg, and 1.3 and 9.2 g/kg, respectively. Tuna oil supplementation was associated with significant adverse effects on flavour and overall acceptance of bacon (not significant in LD although numerically the same trend was noted), but these effects on sensory ratings were limited in extent. Also shelf life of the products, determined as TBA value after different storage periods at $4^{\circ}C$ in LD, backfat and bacon, was significantly reduced. Overall, the present study suggests that omega-3 fatty acids may be enriched in pork by feeding tuna oil with few undesired side-effects, particularly those on sensory perception and shelf life, suggesting immediate consumption of the products is advisable. Most economically important traits (performance, slaughter and physical meat quality) remained unaffected.

EFfect of Feed Withdrawal Time on Salmonella Contamination in the Crop, Intestinal Weight, and Blood Parameters of Broilers before Harvesting (절식 시간에 따른 육계의 소낭 내 Salmonella 오염도, 소화관 길이, 혈액 성분, 계육 품질에 미치는 영향)

  • Kim, Hee-Jin;Kang, Hwan-Ku;Hong, Eui-Chul;Kim, Hyun-Soo;Son, Jiseon;You, Are-Sun;Kang, Bo-Seok;Jeon, Jin-Joo
    • Korean Journal of Poultry Science
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    • v.48 no.4
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    • pp.287-295
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    • 2021
  • In this study, we aimed to evaluate the impact of different feed withdrawal periods (2, 3, 4, 5, and 6 h) on Salmonella contamination in the crop, intestinal weight, and blood parameters of broilers before harvesting. A total of 100 broilers (initial live weight 1.906±0.06 kg) were randomly assigned to the same five treatment groups and broilers had access to water but no feed before catching. Feed withdrawal times were 2, 3, 4, 5, and 6 h prior to slaughter. Measurements included live weight, pre-slaughter weight, carcass weight, weight of gastrointestinal tract. In addition, Salmonella and Campylobacter contamination in the crop, serum biochemical profiles, counts of leukocytes, erythrocytes, and platelets, and meat quality were assessed. The results demonstrate that carcass weight, blood corticosterone, meat pH, meat color, and water holding capacity did not differ based on the feed withdrawal time. Carcass yield increased as the withdrawal time increased, but the weights of the crop, proventriculus, jejunum, ileum, and rectum were significantly reduced by 6 h (P<0.05). Salmonella counts were the lowest in the crops subjected to 6 h of feed withdrawal (P<0.05). Shear force was highest in chicken breast meat after 6 h of feed withdrawal (P<0.05). However, when the feed was withdrawn after 6 h, the shear force increased, but it did not affect the sensory characteristics in consumers, and the Salmonella count also decreased and had a reduction effect on contamination during slaughter. These findings can serve as reference data for setting the feed withdrawal time.

Effects of different space allowances on growth performance, blood profile and pork quality in a grow-to-finish production system

  • Jang, J.C.;Jin, X.H.;Hong, J.S.;Kim, Y.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.12
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    • pp.1796-1802
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    • 2017
  • Objective: This experiment was conducted to evaluate the optimal space allowance on growth performance, blood profile and pork quality of growing-finishing pigs. Methods: A total of ninety crossbred pigs [$(Yorkshire{\times}Landrace){\times}Duroc$, $30.25{\pm}1.13kg$] were allocated into three treatments (0.96: four pigs/pen, $0.96m^2/pig$; 0.80: five pigs/pen, $0.80m^2/pig$; 0.69: six pigs/pen, $0.69m^2/pig$) in a randomized complete block design. Pigs were housed in balanced sex and had free access to feed in all phases for 14 weeks (growing phase I, growing phase II, finishing phase I, and finishing phase II). Results: There was no statistical difference in growing phase, but a linear decrease was observed on average daily gain (ADG, p<0.01), average daily feed intake (ADFI, p<0.01), and body weight (BW, p<0.01) with decreasing space allowance in late finishing phase. On the other hand, a quadratic effect was observed on gain to feed ratio in early finishing phase (p<0.03). Consequently, overall ADG, ADFI, and final BW linearly declined in response to decreased space allowance (p<0.01). The pH of pork had no significant difference in 1 hour after slaughter, whereas there was a linear decrease in 24 h after slaughter with decreasing space allowance. Floor area allowance did not affect pork colors, but shear force linearly increased as floor space decreased (p<0.01). There was a linear increase in serum cortisol concentration on 14 week (p<0.05) with decreased space allocation. Serum IgG was linearly ameliorated as space allowance increased on 10 week (p<0.05) and 14 week (p<0.01). Conclusion: Data from current study indicated that stress derived from reduced space allowance deteriorates the immune system as well as growth performance of pigs, resulting in poor pork quality. Recommended adequate space allowance in a grow-to-finish production system is more than $0.80m^2/pig$ for maximizing growth performance and production efficiency.