• Journal of the Society of Cosmetic Scientists of Korea
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• v.36 no.4
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• pp.315-321
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• 2010

In this study, we investigated the antibacterial activity and the skin moisturizing effect of cream contaning Cedrela sinensis A. Juss shoots extracts. MIC values of ethyl acetate fraction from Cedrela sinensis A. Juss shoots extracts on P. acnes and S. aureus were 0.13 %, 0.03% respectively and P. ovale and E. coli were 0.25 % equally. The results showed that the antibacterial activity of the ethyl acetate fraction on P. acnes and S. aureus were higher than the methyl paraben. The cream containing the ethyl acetate fraction from Cedrela sinensis A. Juss shoots extracts was formulated and its skin moisturizing effect was evaluated in a clinical trial. The skin hydration and transepidermal water loss (TEWL) were investigated after topical application of the cream. The skin hydrating effect of the cream containing Cedrela sinensis A. Juss shoots extract was 10 ~ 15 % higher than the placebo cream, and transepidermal water loss was decreased to 6.7 g/$m^2h$ in the subjects applied with cream containing the ethyl acetate fraction of Cedrela sinensis A. Juss shoots extracts. These results indicate that ethyl acetate fraction from Cedrela sinensis A. Juss shoots extracts could be applicable to new functional antibacterial cosmetics.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.18 no.3
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• pp.44-54
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• 2005

Background and Objectives: Allergic contact dermatitis is a common environmental health issue and seriously affect the patient's quality of life. The more our environment industrialized, the number of material that could cause the allergic contact dermatitis has been increased, consequently the prevalence of allergic contact dermatitis has been increased. In oriental medicine, clinically Chogam-Tang has been used fur the treatment of allergic dermatitis, eczema, atopic dermatitis etc. The objective of this study is to investigate the effects of Chogam-Tang on allergic contact dermatitis Meterial and Methods: Fifteen Sprague-Dawley rats were divided into three groups: normal, control, experimental group. Control and experimental group were induced allergic contact dermatitis, by DNCB. Experimental group was orally administered the Chogam-Tang. Each group was observed after 24, 48 and 72 hours. Contact hypersensitivity assay, melanin-erythema measurement, pH measurement skin moisture measurement and biopsy were performed. Results: 1. In contact hypersensitivity assay, experimental group showed decreased ear swelling compared with control group at 48hours. 2. ln melanin measurement there was no difference in three groups. 3. In erythema measurement experimental group showed reduction at 48. 72 hours. 4. In pH measurement, experimental group and control group showed increase in pH but there was no statistical significance. 5. In skin moisture measurement, experimental group showed higher skin moisture level than control group at 24 hours and showed lower skin moisture level at 72 hours, but there was no statistical significance. 6. In biopsy, experimental group showed decrement of Iymphocyte as time goes by, and regeneration of keratin layer was increased compared with normal group. Conclusions: Chogam-Tang shows anti-inflammatory effect in biopsy, improves hydration levels of skin, decreases erythema level on allergic contact dermatitis.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.32 no.3
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• pp.23-36
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• 2019

Objectives : The purpose of this study is to confirm the efficacy and safety of "Functional cosmetics containing peptide Scolopendrasin-I isolated from the Scolopendra subspinipes mutilans" on dry skin due to mild atopic dermatitis. Methods : A total of 24 patients who visited Semyung Oriental Medical Center from November 1st, 2018 to December 20th, 2018 were included in the study. In this study, the patients were treated with Functional cosmetics containing peptide Scolopendrasin-I isolated from the Scolopendra subspinipes mutilans. For 4 weeks of gross examination, instrumental assessment were made before and after the study to evaluate how well the products for treatment group in recovering the dry skin barriers by mild atopic dermatitis. Results : 1. In the primary endpoint, Skin Hydration showed a statistically significant increase in treatment group in baseline, 2 weeks and 4 weeks. 2. In the primary endpoint, Transepidermal Water Loss(TEWL) showed a statistically significant decrease in treatment group between Baseline and 4 weeks. 3. In the secondary endpoint, Skin pH showed a statistically significant decrease in treatment group between baseline and 4 weeks. 4. To evaluate the safety of the products for the human body, Adverse events, SCORAD Index Assessment were conducted; There were no severe adverse events during this study. And SCORAD Index showed a statistically significant decrease in treatment group in baseline, 2 weeks and 4 weeks. Therefore, it is suggested that products, if used for certain period, should be safe for the human body. Conclusions : According to the above experiments, it is suggested that "Functional cosmetics containing peptide Scolopendrasin-I isolated from the Scolopendra subspinipes mutilans" should be effective for dry skin due to mild atopic dermatitis.

• Journal of Korean Medical classics
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• v.12 no.1
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• pp.113-138
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• 1999

Distension of hydrops is the pathosis that the hydration is retented in abdomen and that is caused by disturbance of hydrometabolism. Most of hydrops belongs to Consolidation. In part of OhRyungJinAek-Byeul of Youngchu"靈樞 五癃津液別", the general explanation of hydrops is briefed. But there was nothing about pathogenesis, pathology, symptoms and the method of treatment. The part SuChang of Youngchu "靈樞 水脹" divided it by Superficial-swelling, Hidden-swelling, Ovarian-hydrops, Stony-uterin-mass and explained it to cure by Category of pathology. So, the books JeByeongWonHuRon"諸病源候論" ChonGeumYoBang"千金要方", WoiDaeBiYo"外臺秘要", TaePyeungSeongHyeBang"太平聖惠方", BoJeBang"普濟方", EuiHakGangMok"醫學綱目", DongEuiBoGam"東醫寶鑑" etc. published later, show the method of treatments and medications with quotation the original text of this part. And it is convinced that more profound research of this part is inevitable to understand the correct meaning of hydrops that explained by many of ancient medical scholars, and to apply it to clinical medicine. There is an explanation on Distension hydrops in the prologue of this part. And t six kinds of disease in this part is simila to edema, so it is called SuChang"水脹", pathology of Edema. Superficial-swellin Hidden-Swelling is reported in the fir chapter, differential diagnosis of Ovarian-hydrops, Stony-uterin-mass is rep in the second chapter, and treatment Superficial swelling. Hidden Swelling reported in the third chapter. Briefly looking over that chapter, Distension of hydrops is recognized as stay of hydration at the abdomen as a result of disturbance of hydrometabolism and it says about symtoms, pathogenesis, pathology, differential diagnosis, treatment of Hydrops Superficial-swelling Hidden-swelling Ovarian-hydrops Stony-uterinmass. And it says about a few things that Superficial-swelling, Hidden-swelling could be differentiated by colors of skin and distension of abdomen, and Ovarian-hydrops, Stony-ulerin-mass could be differentiated that it occurs which sex and whether mensturation occurs or not, and treatment of Superficial-swelling, Hidden-swelling, Ovarian-hydrops.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.33 no.1 s.60
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• pp.17-22
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• 2007

In this study, we investigated the effects of Sedum sarmentosum extract on the expression of hyaluronan synthase (HAS) genes and hyaluronan (HA) production in HaCaT keratinocytes. We also assessed water content (electrical capacitance) and transepidermal water loss (TEWL) in human skin after topical treatment with Sedum sarmentosum extract. Sedum sarmentosum extract increased expression of HAS-2 and HAS-3 genes in HaCaT cells, when assayed by real-time reverse-transcriptase polymerase chain reactions (RT-PCR). Sedum sarmentosum extract induced HA production in HaCaT cells, when determined by enzyme-linked immunosorbent assay (ELISA). Finally, treatment of Sedum sarmentosum extract on human skin increased the skin hydration and decreased TEWL when measured using Corneometer and Tewameter. Our study suggests that Sedum sarmentosum extract should be a very useful cosmetic ingredient, as a skin moisturizer.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.39 no.3
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• pp.233-239
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• 2013

In this study, water content, sebum content, and transepidermal water content were investigated to provide basic data for the future cosmetics industry as comparing the skin characteristics of different ages. Water content of cheek area, sebum content of nose area, and transepidermal water loss (TEWL) of cheek and forearm of trial subjects (total 638 people of both male and female) were measured. Data were analyzed using Statistical Package for the Social Sciences program (version 19.00; SPSS Inc., Chicago, IL, USA). Water content, sebum content, and TEWL of skin were measured as mean ${\pm}$ standard deviation. As a result, water content of cheek from female was higher than male and the water content increased with the older ages. However, sebum content of nose from male was higher than female and the sebum content increased with the younger ages. TEWL of cheek from female was higher than male, but TEWL of forearm from male was higher than female. Therefore, these results can be used as basic data in the development of future cosmetics for different ages and genders.

• Applied Microscopy
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• v.38 no.3
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• pp.167-174
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• 2008

Atopic dermatitis (AD) is a chronically relapsing inflammatory skin disease that often has asthma and allergic rhinitis. Magnesium salts, the important component of minerals in Dead Sea water, are known to exhibit beneficial effects in inflammatory disease. Favorable effects of magnesium ions and sea water treated to the skin of patients with contact dermatitis have been reported. But histological and immunological investigations are insufficient. This study was performed to examine the inhibitory effect of magnesium-rich sea mineral water on the development of AD-like skin lesions in hairless mice. AD-like skin lesions are induced by the repeated application of 2,4-dinitrochlorobenzene (DNCB). Local application of magnesium-rich sea mineral water on hairless mice skin applied with DNCB inhibited the development of AD-like skin lesions as exemplified by a significant increase in skin hydration (p<0.01), and a decrease in epidermal water loss (p<0.01). Serum IgE level was also significantly decreased (p<0.01). These results suggest that magnesium-rich sea mineral water inhibits the development of DNCB-induced AD-like skin lesions in hairless mice. These observations indicate that magnesium-rich sea mineral water may be alternative and assistant substances for the management of AD.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.1 s.49
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• pp.97-102
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• 2005

One of the key molecules involved in skin moisture is hyaluronan (hyaluronic acid, HA) with its associated water of hydration. The predominant component of the ECM (extracellular matrix) of skin is HA. It Is the primordial and the simplest of the GAGs (glycosaminoglycans), a water-sorbed macromolecule In extracellular matrix, Included between the vital cells of epidermis. In the skin, HA was previously thought to derive extlusively from dermis. But, recent studies revealed that HA could be synthesized in epidermis. Flavonoids are polyphenolic compounds that is found mainly in foods of plant origin. Kaempferol was known to increase glutathione synthesis in human keratinocyte. And quercetin blocked PPAR-meidated keratinocyte differentiation as lipoxygenase inhibitors. In this study, we sought to evaluate the effect of flavonid, kaempferol and quercetin on production HA in keratinocyte. We examined the changes of three human hyaluronan synthase genes (HASI, HAS2, HAS3) expression by semi-quantitative RT-PCR when kaempferol or quercetin was added to cultured human keratinocytes. We found that these flavonoids slightly upregulated HAS2, HAS3 mRNA after 24 h. And we investigated the effect on HA production by ELISA. When we evaluated the level of HA in culture medium after 24 h incubation. We found enhanced accumulation of HA in the culture medium. Although the effects of above flavonoids are less than retinoic acid, the data indicate that kaempferol, quercetin can dose-dependently increase the level of HA in epidermis cell line. It suggested that flavonoid, kaempferol, and quercetin increased production of HA in skin and it helped to elevate skin moisture and improve facial wrinkle.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.27 no.3
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• pp.40-55
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• 2014

Objectives : The purpose of this study is to confirm the effects of external applications(CLANCHE SM A Clinic) containing herbal extracts on patient with atopic dermatitis. Methods : A total of 24 patients who visited Semyung Oriental Medical Center from January 15th, 2014 to February 20th, 2014 were included in the study. In this study, the patients were treated with external applications(CLANCHE SM A Clinic) containing herbal extracts. For 4 weeks of gross examination and instrumental assesment were made before and after the study. Results : 1. In the primary endpoint, SCORAD index showed a statistically significant decline. 2. In the secondary endpoint, index of skin hydration and index of TEWL showed statistical significance. 3. In the secondary endpoint, index of skin pH showed no statistically significant result. 4. To evaluate the safety of the products for the human body, vital sign check were conducted and showed no statistically significant result. And There were no severe adverse events during this study. Therefore, it is suggested that the safety of the products, if used for certain period, should be safe for the human body. 5. In global assesment of efficacy, both experimenter and subjects evaluated external applications were effective. Conclusions : According to the above experiments, it is suggested that external applications(CLANCHE SM A Clinic) containing herbal extracts should be effective for the atopic dermatitis.

• Journal of Convergence for Information Technology
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• v.10 no.5
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• pp.232-238
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• 2020

This study evaluated the anti-aging activity with antioxidant, anti-inflammatory and moisture activity of Ganoderma lucidum spore oil(GLS). GLS increased DPPH radical scavenging activity in a dose-dependent manners. Anti-inflammatory assay measured the inhibitory effect of GLS on NO, TNF-α and IL-6 production in LPS-stimulated RAW264.7 cells. As a result GLS inhibited NO and pro-inflammatory cytokine, TNF-α, IL-6 production. Also using human fibroblast cell to the procollagen production analysis and COL1A1 mRNA expression level analysis for defining, and for AQP-3 mRNA expression level analysis, used human keratinocyte cell. GLS increased procollagen production and COL1A1, AQP-3 mRNA expression. Our results suggest that the GLS have potential anti-inflammatory and wrinkle improves, skin moisture effect.