• Journal of the Korean Applied Science and Technology
• /
• v.33 no.2
• /
• pp.361-373
• /
• 2016

This study tested the antioxidant activities and anti-aging effects of an extract of Orostachys japonicus, a herb used as a folk remedy for cancer for a long time. To assess the antioxidant activities of the Orostachys japonicus extract (OJE), the total polyphenol content was confirmed to have a high caffeic acid equivalent weight of 12 g by dry weight of 100g OJE and the total flavonoid content was confirmed that the quercetin equivalent of 1.7 g by dry weight of 100g OJE. DPPH free radical scavenging activity was confirmed a high scavenging activity of 79.7% at a concentration of 1%. To confirm the anti-aging effect of OJE, MMP-1 expression was decreased in $25{\mu}L/mL$ and $50{\mu}L/mL$ similar to control. In a clinical experiment, a group of subjects who used a cosmetic product containing OJE showed a significant increase in the amount of skin moisture from the forehead, left cheek, and chin. The experimental group showed a significant increase in the amount of skin sebum from the chin and maintaining pH balance of normal skin, As a result, the OJE demonstrates the efficacy of natural cosmetic material as antioxidant and anti-aging.

• KSBB Journal
• /
• v.23 no.5
• /
• pp.431-438
• /
• 2008

For the experiment, to develop new materials for cosmetics, the Celosia cristata L. plant ethanol extract were used for physiological effect and cosmetics application research. The Celosia cristata L. is a Korean traditional variety grown. To investigate the effect of Ethanol extract of Celosia cristata L. on skin care, we measured anti-oxidant activity and anti-aging activity. Celosia cristata L. ethanol extract itself had anti-oxidant activity in a dose-dependent manner in 1-diphenyl-2-picryl-hydrazyl(DPPH) radical scavenging. Ethanol extract had anti-oxidant activity in a dose-dependent manner. Silica dose-dependently increased the intracellular ROS generation in RAW 264.7 cells. Celosia cristata L. ethanol extract inhibited silica-induced intracellular superoxide anion generation and $H_2O_2$ generation and hydro-peroxide generation in RAW 264.7 cells. For anti-aging effects, the hyaluronidase inhibition effects, were relatively strong and they also showed elastase activity inhibition effects, which suggesting the Celosia cristata L. ethanol extract might be used as hydration and anti-wrinkle agents. From the above results, it is referred that Celosia cristata L. ethanol extract appears to have potent anti-oxidant activity and anti-aging activity.

• The Korea Journal of Herbology
• /
• v.38 no.5
• /
• pp.85-95
• /
• 2023

Objectives : This study was intended to reveal the chemical profiles of aerial(leaf, stem) and underground(rhizome, radix) parts of wild ginseng, and to investigate their anti-aging effects on human skin cells. Methods : Wild ginseng, estimated for over 20 years, was divided into the aerial and underground parts. Total phenolic contents of each extracts were measured using a Folin-ciocalteu method. The contents of 18 amino acids, 8 minerals and 27 ginsenosides were determined by GC-FID, ICP-MS and LC-MS, respectively. The anti-aging effects, including the radical scavenging activity, the activation of mitochondrial function on human fibroblasts, and the proliferation activity on human keratinocyte progenitor cells, for the whole plant and underground part of wild ginseng were evaluated. Results : The total phenolic acids, amino acids, and minerals in the aerial part were more than twice as high as in the underground part. Compared to the cultivated ginseng root, there were various types of ginsenosides in both parts of wild ginseng, and the total amount was more than twice as high. In particular, the aerial part significantly contained ginsenoside F1, F2, C-Mc1, and C-O, and the distinctive patterns that distinguish each parts of wild ginseng from the cultivated ginseng root were derived. The whole plant and underground part of wild ginseng exhibited significant antioxidant effect(14.3-45.6%), activation of mitochondrial membrane potential(105.5-120.1%), and cell proliferation(112.1-125.4%). Conclusions : The entire plant and underground part of wild ginseng are high value-added plants and have beneficial effects on skin anti-aging properties through its abundant metabolites.

• Journal of Haehwa Medicine
• /
• v.21 no.2
• /
• pp.63-72
• /
• 2013

To develop a new anti-skin aging cosmetics or functional foods by using antioxidative activity and collagenase inhibitor, a potent collagenase or elastase inhibitor was screened from various extracts of medicinal plants and its optimal extraction condition was investigated. And antioxidative activity, antimicrobial activity and inhibitory of effect against collagenase activity were investigated. In the these results, we selected the Sanguisorba (Sanguisorba officinalis L.) that presents a potential biological activities. Sanguisorba which is very rich in triterpenoid saponin and tannins was recently reported its anti-oxidant activities and phytoestogenic activities in vivo test and many clinical studies. The experiments were carried out in vitro to determine anti-oxidant activities of Sanguisorba extracts on DPPH radical scavenging activity assay, Superoxidase scavenging activity assay, Elastase and collagenase activity assay. It show that the Sanguisorba extracts have the most significant anti-oxidant on free radical scavenging activity assay, and also inhibited significantly activities of elastase, collagenase. Further, Sanguisorba extracts are activated Type I collagen protein expression in CCD-986sk cells. These result suggest that the Sanguisorba extracts on DPPH radical scavenging activity assay, Superoxidase scavenging activity assay, elastase and collagenase activity assay, Type I collagen protein expression in CCD-986sk cells effected could be developed cosmetic ingredients for anti-aging.

• Journal of the Korean Society for Nondestructive Testing
• /
• v.25 no.5
• /
• pp.368-376
• /
• 2005

Coolant rubber hoses for automobile radiators can be degraded and thus failed due to the influence of contacting stresses of air and coolant liquid under thermal and mechanical loadings. In this study, for EPDM(ethylene-propylene diene monomer) rubber conventionally used as a radiator hose material the aging behaviors of the skin part due to thermo-oxidative and electro-chemical stresses were nondestructively evaluated. Through the thermo-oxidative aging test, it was shown that the surface hardness IRHD(International Rubber Hardness Degrees) of the rubber increased with a considerable reduction of failure strain. On account of the penetration of coolant liquid into the skin part the weight of rubber specimens degraded by electro-chemical degradation(ECD) test increased, whereas their. failure strain and IRHD hardness decreased largely. The penetration of coolant liquid seemed to induce some changes in inner structure and micro hardness distribution of the rubbers. Consequently, EPDM rubbers degraded by thermo-oxidative aging and ECD could be characterized nondestructively by micro-hardness and chemical structure analysis methods.

• Journal of Nutrition and Health
• /
• v.49 no.6
• /
• pp.429-436
• /
• 2016

Purpose: Solar ultraviolet (UV) radiation causes inflammation and matrix metalloproteinase (MMP) overexpression and extracellular matrix depletion, leading to skin photoaging such as wrinkle formation, dryness, and sagging. Activation of MMP is influenced by various molecules such as reactive oxygen species (ROS), proinflammatory cytokines, and transient receptor potential vanilloid type (TRPV)-1, which are increased in UV-irradiated skin cells. Aralia elata (AE) ethanolic extract was reported to inhibit ROS generation caused by UVB-irradiation in keratinocytes. In this study, we investigated the photoprotective effect of AE ethanolic extract on UVB-irradiated human keratinocytes (HaCaT) and human dermal fibroblasts (HDF). Methods: AE was freeze-dried, extracted in 70% ethanol, and concentrated. Skin cells were treated with AE extract for 24 h and then exposed to UVB ($55mJ/cm^2$). After 48 h of incubation, proinflammatory cytokines, MMP-1, type-1 procollagen, and TRPV-1 levels were measured by ELISA or Western blotting. Results: Treatment with AE extract ($100{\mu}g/mL$) significantly inhibited UVB-induced IL-6, IL-8, and $PGE_2$ production in HaCaT by 25.6%, 5.3%, and 70.2%, respectively, and also inhibited elevation of MMP-1 and TRPV-1 caused by UVB irradiation by 20.0% and 41.9%, respectively (p < 0.05). In HDF, AE extract treatment significantly inhibited both elevation of MMP-1 and reduction of type-1 procollagen caused by UVB irradiation (p < 0.05). In addition, type-1 procollagen was elevated by AE extract treatment in normal HDFs (p < 0.05). Conclusion: AE 70% ethanol extract has photoprotective ability via reduction of proinflammatory mediators, TRPV-1 and MMP-1 production, and elevation of collagen synthesis. Our findings suggest that AE extract might be a good natural material to protect against UVB-induced premature skin aging.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.34 no.4
• /
• pp.1-11
• /
• 2021

Objectives : The aim of this study was to investigated the anti-aging and anti-wrinkles effects of the pinus koreaiensis seed(PKS) oil. Methods : The anti-oxidant effect was performed by beta-carotene bleaching assay and the intracellular proteome was analyzed expression of each 15 proteins by 2-D electrophoresis. And fatty acid was analysed by gas chromatography. Anti-wrinkle effect was analyzing human skin by the PRIMOS system. Results : Fatty acid analysis of PKS oil has shown oleic acid was 49.7% and linoleic acid was 34.1%. And the antioxidant effect was about 125% compared with alpha-tocoperol(0.1%) by beta carotene bleaching assay. In 2D PAGE analysis, fifteen protein changes in five mechanisms which was collagen synthesis pathway, MMPs, ECM-cell interaction, cytokine, antioxidant enzymes were analyzed. In case of anti-wrinkle effect was proved in vivo by analyzing human skin by the PRIMOS system. The analysis results of eye wrinkles for 4 weeks showed an improvement effect of over 6%. Conclusions : In this study, the amount of protein change in the five mechanism through the cell experiment and the skin anti wrinkle efficacy by the human in vivo test were investigated. As a result pinus koreaiensis seed oil by supercritical extraction could be used as a anti-aging and anti-wrinkle substance for the skin.

• Journal of Life Science
• /
• v.30 no.9
• /
• pp.819-825
• /
• 2020

All living organisms exhibit the characteristics of aging, such as skin wrinkle formation, muscle degeneration, cataracts, and hair graying as the number of aged cells increases over time. Senescence, which is known as a key cause of aging, is directly related to the aging of living organisms because cells are aged by external and internal factors and eventually cell proliferation is stopped. Senescence is caused by the gradual shortening of the telomere with cell division, and lifespan is determined by the length of the telomere. Recently, it has been found that the histone deacetylase, which can influence gene expression, is not only involved in yeast but also deeply involved in anti-aging mechanisms in both C. elegans and humans. It was also discovered that old cells play a decisive role in the aging phenomenon, and it has been reported that it is possible to promote the proliferation of young cells and delay aging by removing these senescent cells from the inside. Therefore, in order to develop potential anti-aging agents in the future, research should begin with an in-depth study of telomerase activators, sirtuin activators, and senolytics.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.30 no.4 s.48
• /
• pp.445-448
• /
• 2004

Aging is divided into intrinsic aging and photo-aging. Intrinsic aging is naturally occurred as the time passed and photo-aging is induced by the UV radiation of skin. The main reason of aging is the free radicals and the degeneration of the cellular materials by free radicals. In this paper, we checked the anti-aging effects of Mallotus japonicus bark extracts. It has the ability to scavenge free radicals and the SOD like activity. Also, it reduced the cell damage by hydrogen peroxide treatment. Mallotus japonicus bark extracts showed the excellent activity on inhibiting the UV induced cell damage and DNA damage. In conclusion, Mallotus japonicus bark extracts can be used as active ingredients for anti-aging cosmetics.

• Fashion & Textile Research Journal
• /
• v.15 no.6
• /
• pp.993-999
• /
• 2013

This study aimed to measure the amount of sweating on 12 parts of the upper body using absorption fabric and analyze subjective sensations. The study was conducted with 9 male subjects in climate chamber controled at $30{\pm}0.5^{\circ}C$, and $55{\pm}5%$ RH. The result was that sweating amount of the upper back part was significantly more than upper front part. We assumed that forced convection flow cased by exercise decreased the sweating rate in the front. The skin temperature of upper front body rapidly decreased as soon as exercise starts and gradually increased with cessation of exercise. On the other hand, the skin temperature of palm increased with exercise and showed continuous increasing even exercise stopping all the experimental period. This is caused by thermoregulatory responses through vasodilatation on the peripheral area. Subjective sensations, such as thermal sensation, wet sensation, and thermal comfort showed the highest score at the time of exercise stop. This means the subjects felt more hot, wet, and uncomfortable after exercise stopped. Bur after wiping of sweat, subjective sensation scores were recovered rapidly. The present study has provided more detailed information on the upper body sweat distribution than previously available, which can be used in clothing design, thermo-physiological modeling, and thermal manikin design. We also think that results of the present study will play an important role in making the sweat distribution map.