• 한국약용작물학회지
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• 제13권6호
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• pp.213-216
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• 2005

본 연구에서는 비교적 가격이 저렴하고 부작용이 없으면서 효능이 우수한 피부 노화 억제 제품을 개발하고자 먼저 다양한 종류의 약용식물을 대상으로 각종 유기용매 추출물을 제조한 후 이들의 elastase 저해 활성을 측정하여 우수 식물을 선발하고 이들로 부터 elastase 저해제 추출 최적조건을 검토하였다. 64종의 약용식물에 대한 다양한 추출물 가운데 복분자 메탄올 추출물의 elastase 저해 활성이 85%로 가장 높았고 이 저해물질은 메탄올로 $50^{\circ}C$에서 12시간 진탕 시켰을 때 가장 많이 추출 되었다.

• Journal of Microbiology and Biotechnology
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• 제30권10호
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• pp.1567-1573
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• 2020

Ultraviolet (UV) is one of the major factors harmful to skin health. Irradiation with ultraviolet accelerates the decline of skin function, causing the skin to have deep wrinkles, dryness, decreased procollagen production, and degradation of collagen. Novel materials are needed to prevent the aging of the skin by blocking the effects of UV. Safflower seed oil (Charthamus tinctorius L., SSO) contains significantly high levels of unsaturated fatty acids and phytochemicals. SSO has been traditionally used in China, Japan, and Korea to improve skin and hair. Our objective in this study was to determine the effect of SSO and its active compound acacetin on UVB-induced skin photoaging in HaCaT cells and human dermal fibroblasts (HDF). SSO inhibited UVB-induced matrix metalloproteinase-1 (MMP-1) at both protein and mRNA levels in HaCaT cells and HDF. MMP-1 is known to play important roles in collagen degradation and wrinkle formation. Acacetin, a type of flavonoid, is present in SSO. Similar to SSO, acacetin also inhibited UVB-induced MMP-1 protein and mRNA levels in HaCaT cells and HDF. MMP-1 mRNA is primarily regulated by the mitogen-activated kinase (MAPK) signaling pathway. Acacetin regulated the phosphorylation of JNK1/2 and c-jun, but did not inhibit the phosphorylation of ERK1/2, p38 and AKT. Taken together, these results indicate that SSO and its active compound acacetin can prevent UVB-induced MMP-1 expression, which leads to skin photoaging, and may therefore have therapeutic potential as an anti-wrinkle agent to improve skin health.

• Biomolecules & Therapeutics
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• 제31권6호
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• pp.640-647
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• 2023

The skin, the largest organ in the body, undergoes age-related changes influenced by both intrinsic and extrinsic factors. The primary external factor is photoaging which causes hyperpigmentation, uneven skin surface, deep wrinkles, and markedly enlarged capillaries. In the human dermis, it decreases fibroblast function, resulting in a lack of collagen structure and also decreases keratinocyte function, which compromises the strength of the protective barrier. In this study, we found that treatment with γ-aminobutyric acid (GABA) had no toxicity to skin fibroblasts and GABA enhanced their migration ability, which can accelerate skin wound healing. UVB radiation was found to significantly induce the production of matrix metalloproteinase 1 (MMP-1), but treatment with GABA resulted in the inhibition of MMP-1 production. We also investigated the enhancement of filaggrin and aquaporin 3 in keratinocytes after treatment with GABA, showing that GABA can effectively improve skin moisturization. In vivo experiments showed that oral administration of GABA significantly improved skin wrinkles and epidermal thickness. After the intake of GABA, there was a significant decrease observed in the increase of skin thickness measured by calipers and erythema. Additionally, the decrease in skin moisture and elasticity in hairless mice exposed to UVB radiation was also significantly restored. Overall, this study demonstrates the potential of GABA as functional food material for improving skin aging and moisturizing.

• Archives of Plastic Surgery
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• 제33권4호
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• pp.525-529
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• 2006

Purpose: Facial nerve injury is a rare but feared complication of face lift. Uncertainty as to the depth and vulnerability of the facial nerve in face lift dissection causes some surgeon, particularly novices, to dissect slowly. Excessively thin flaps can be made because of fear of nerve injury, contributing to skin slough. Methods: From September 1998 to February 2003, the authors operated on 34 aging face patients using classical face-lift. We had analysed about quantity of skin removal and degree of elevated flap. The authors have found quantity of skin removal was 1.5-2.0 cm, degree of elevated flap was 40-45 degree on average. Results: The authors performed preauricular pre-excision face-lift technique on 12 aging face patients from July 2003 to Feburary 2005 based on experienced surgery. This technique reduced fear of dissecting skin flap necrosis and facial nerve injury because of firmly attached pre-auricular skin removed in advance. Conclusions: We easily dissected SMAS without visual field disturbance, nerve damage and reduced operation time and bleeding loss compared to classical face-lift.

• Bulletin of the Korean Chemical Society
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• 제28권1호
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• pp.99-102
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• 2007

To prepare freeze-dried ascorbyl palmitate (AsP)-containing liposome which can protect the drug from moisture attack and be used instantly by mixing with water for anti-aging and skin whitening therapy, AsP was encapsulated into liposomes and freeze-dried with trehalose. The freeze-dried liposome formulations were characterized by measuring water contents, particle size, time required for complete reconstitution. With the freeze-dried liposomes, we performed the stability test under accelerated conditions, skin permeation and localization test. The measurement of the time to perfect reconstitution showed that the freeze-dried liposomes can be changed to their initial state rapidly and short term stability test of AsP in reconstituted liposomes under accelerated conditions confirmed that the stability of AsP was considerably enhanced as compared to freshly prepared liposomes. The skin permeation and localization properties of AsP in reconstituted liposomes were not significantly different, indicating that the liposomal structures were maintained before and after freezedrying. In conclusion, the freeze-drying method provided a possible way to overcome the instability issue of AsP induced by the moisture and reproduced similar skin permeation and localization properties as shown by freshly prepared liposomes.

• Journal of Platform Technology
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• 제10권3호
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• pp.3-10
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• 2022

The effectiveness of an innovative skin treatment system that delivers an anti-aging solution deep into the skin without invasiveness and pain using a non-invasive air technology was investigated. In addition, an effective change using a non-invasive technique for delivering a solution for skin improvement was confirmed. The equipment named Cellre Jet is an effective skin care and drug delivery equipment that instantly opens the skin epidermis by using a maximum output pressure of 6 bars and high-pressure purified oxygen of 75-90% purity to deliver various nano-sized vital substances deep into the skin, and it uses the method of precisely controlling the equipment through an 8-inch digital touch display to accurately dispense the prescribed dosage. In this study, changes in skin condition were analyzed using this equipment and nano ampoules on subjects with actual skin problems through a related comparison and effectiveness judgment program. Through this study, skin care and drug delivery are possible, which will contribute to verifying the effectiveness of this non-invasive drug delivery equipment in the future, and is expected to establish the systematic effect in observing and studying changes in the skin.

• Journal of Ginseng Research
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• 제40권3호
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• pp.260-268
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• 2016

Background: During the aging process, skin shows visible changes, characterized by a loss of elasticity and the appearance of wrinkles due to reduced collagen production and decreased elasticity of elastin fibers. Panax ginseng Meyer has been used as a traditional medicine for various diseases due to its wide range of biological activities including skin protective effects. Ginsenosides are the main components responsible for the biological activities of ginseng. However, the protective activities of an enzymatic preparation of red ginseng against human skin aging have not been investigated. Methods: The efficacy of an enzyme-treated powder complex of red ginseng (BG11001) in preventing human skin aging was evaluated by oral administration to 78 randomized individuals. All patients were requested to take three daily capsules containing either 750 mg of BG11001 or a placebo vehicle for 24 wk; at the end of the testing period, skin roughness, elasticity, and skin water content were measured. Results: BG11001 significantly reduced the average roughness of eye wrinkles and the Global Photo Damage Score compared with the placebo, although there were no significant differences in arithmetic roughness average between the groups. In addition, gross elasticity and net elasticity values increased, and transepidermal water loss level decreased, indicating improved skin elasticity and moisture content. Conclusion: In conclusion, enzyme-treated red ginseng extract significantly improved eye wrinkle roughness, skin elasticity, and moisture content. Moreover, enzyme-treated red ginseng extract would be useful substance as a bio-health skin care product.

• 대한화장품학회지
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• 제47권2호
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• pp.139-145
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• 2021

식물 유래 exosome-like nanovesicles (plant-derived exosome-like nanovesicles, PELNs)은 다양한 생물학적 활성을 포함하고 높은 생체 적합성을 가지고 있다. 인체 내에서 PELNs은 세포 분화 및 증식 조절에 영향을 미칠 수 있어 여러 산업 분야에서 응용이 가능하다. 하지만, PELNs의 피부 생리적 기능에 대한 연구는 포유류 nanovesicles에 비해 미미한 실정이다. 본 연구에서는 사과 열매로부터 캘러스를 유도하고 exosome-like nanovesicles (Exosome-like nanovesicles isolated from Malus domestica (apple) fruit callus, ACELNs)를 분리하여 피부 장벽 및 피부 노화 개선에 대한 연구를 수행하였다. ACELNs의 수율은 6.42 × 10⁹ particles/mL이였으며, 입자 사이즈는 100 ~ 200 nm 범위로 감지되었다. 인간 유래 피부세포인 HDF cells과 HaCaT cells에서 세포 증식을 유도하였으며, 세포 독성 억제 효과를 보였다. 각질형성능이 유의하게 증가했으며, mRNA levels에서 COL1A1과 FBN1 발현을 증가시켰다. 또한, UVA 조사된 HDF cells에 대한 collagen 합성을 촉진시켰다. 이러한 결과들은 ACELNs가 피부장벽 개선 및 피부노화를 방지할 수 있는 소재로서 활용하기 우수한 소재로 사료된다.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.352-373
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• 2003

Rhamnose-rich (RROP-s) and fucose-rich (FROP-s) oligo-and polysaccharides were prepared and extensively characterised by physical and chemical procedures [1,2] and compared to L-fucose. Their biological properties were then studied on human skin fibroblast cell cultures, human skin explant cultures and on hairless rat skin, using a variety of cell-biological, biochemical and computerised morphometrical procedures. Among the most important properties we could establish, the following are of particular interest for the tretment and prevention of age-dependent modifications of human skin (loss of skin-tissue, cells and matrix, wrinkle formation and others) : stimulation of cell proliferation (by $^3$[H]-thymidine incorporation and the MTT test), scavenging of reactive oxygen species (ROS) using several different procedures, and protease (MMP-2 and MMP-9) down-regulation. A topical preparation, using RROP-s and FROP-s, and/or L-fucose, was shown to increase cell proliferation, dermal matrix synthesis, efficient scavenging of ROS-s and to increase also the thickness of dermal tissue when applied for 4 weeks on hairless rat skin, accompanied by the densification of collagen bundles as well as by an increase of elastin synthesis. Using fluorescent labeled FROPs, it could be shown that these oligosaccharides react with cell-membrane receptors and especially with the elastin-laminin-receptor and the fucose-mannose receptor, but they penetrate also in the cell nucleus, suggesting the possibility of a direct action on the regulation of gene expression. When applied to the human skin of a team of voluntary women encompassing all age-groups, the efficiency of FROP-containing preparation could be confirmed using indentometry and computerised evaluation of skin micro-relief, as well as evaluation of periorbital wrinkles. It appears therefore that these preparations correspond to all the requirements of active anti-aging principles.

• 한국패션뷰티학회지
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• 제2권1호
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• pp.5-13
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• 2004

This study was analyzed on relationship of blood vessel pressure massage and skin and management method on blood vessel pressure for skin care with Yin-yang 5 factors theory, Yin-yang 5 factors theory of blood vessel pressure massage with related Korean medicine is essential for descriptions of the physiology of human body and disease. Six elements(wind, heat, fire, dryness, wetness, and cold) and seven emotions(anger, happiness, thought, worry, sadness, surprise, and fear) effected on skin care and the five viscera and the six stomach. Blood vessel pressure massage related with skin consists of the five viscera and the six stomach and is improved blood circulation and is retarded aging of skin by controls of hormone and free nerve system. Blood vessel pressure massage for skin care improved in the intestine system and blood circulation and got healthy. The blood vessel pressure massage treatment of beauty art can aid the function of bio-rhythm of a human body and make our body health by healing the problems of the five viscera organs and the six stomach. It also help circulate of the blood flow and vigor. The study expects the related researches to improve the various treatments through this treatment. The researcher encountered many problems with the lack of concerned materials and former studies but expects this study to be a study to retard aging the skin and prevent the diseases through the study of the blood vessel pressure massage.