• Journal of Veterinary Clinics
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• v.22 no.4
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• pp.377-381
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• 2005

We tried to extract bone morphogenetic protein (BMP) from the freeze-dried bovine cortical bone (FBCB) for bone graft, which were defatted with chloroform-methanol for 20 days, freeze-dried at $-80^{\circ}C$ for 7 days and sterilized by ethylene oxide gas. Two kg of FBCB were pulverized in a wheel mill to $0.5-2.0mm^3$ cubic in size. The bone particles were demineralized in 0.6N HCI for 10 days at chloroform-methanol$4^{\circ}C$ and defatted with chloroform-methanol for 6 hours at room temperature, which was going to be defatting and demineralized cortical bone (DDM). For extracting BMP, DDM was agitated continuously through 72 hours with magnetic stirrer at $4^{\circ}C$ into 12 times of volume of 6 M guanidine hydrochloride (Gdn-HCl) solution containing proteinase inhibitors to protect BMP such as 2mM N-ethylaleimide, 1mM iodoacetic acid, 1mM phenylmethylsulfonyl fluoride and a sterilizer, 1mM sodium azide. The extraction procedure was repeated for three times. All extracted solution was centrifuged at 10,000 rpm for 30 min and then, the supernatant was dialyzed with 12 times of volume of deionized water at $4^{\circ}C$ for 24-72 hours, which cut off below 6,000-8,000 molecular weight. The dialyzed specimen contained crude-BMP was centrifuged, freeze-dried, and weighted. Through these processing, we could obtained $84.9\%$ as FBCB, $17.8\%$ as DDM and $0.71\%$ as crude-BMP from the wet cortical bone without cancellous bone, marrow and muscles. The crude-BMP were obtained $68.3\%$ from the first extraction, $29.6\%$ from secondary and $2.1\%$ from tertiary, respectively. It was suggested that high yield of crude-BMP migth be explained by three-time repetition of the extraction processing for crude-BMP with Gdn-Hcl sol.

• Korean Journal of Materials Research
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• v.9 no.8
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• pp.768-774
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• 1999

Yttia-doped ceria-stabilized ziconia polycrystals(Ce-TZP) was prepared by dipping method and its microstructure was investigated. By controlling doped-yttria content and annealing condition, yttria-doped Ce-TZP showed the microstructure with irregular grain shape and undulated grain boundary. Irregularity of grain shape increased with the amount of yttria doped, and severe undulated grain boundary was observed mainly at the surface region. In the case of yttria-doped Ce-TZP annealed at 1$650^{\circ}C$ for 2h after two dipping times into yttrium nitrate solution of 0.2M, it showed irregular grain shape both at the surface and at the interior region as well as the most severe irregularity. Hot pressed specimen had mean grain size of 0.3$\mu\textrm{m}$ and undulated grain boundary. All specimens with irregular grain shape were retained the tetragonal phase. The fracture toughness of yttria-doped Ce-TZP with irregular grain shape was over the value of 17.6MPa.m(sup)1/2.

• Journal of the Korean Society for Nondestructive Testing
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• v.20 no.5
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• pp.412-423
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• 2000

By using the relation between stress intensity factor and AE parameter, new approach method for assessing the crack length and detectability of crack was proposed. Laboratory experiment was carried out to identify AE characteristics of fatigue cracks for compact tension specimen. The relationship between a stress intensity factor and AE signals activity as well as conventional AE parameter analysis was discussed. As a result, the features of specific parameter such as the length of crack growth the AE energy, the AE peak amplitude, and the cumulative AE hits, showed the almost same trend in their increase as the number of fatigue cycle increased. From the comparisons of peak amplitude and AE energy with stress intensity factor, it was verified that the higher stress intensity factors generated AE signals with higher peak amplitude and a larger number of AE counts. If we can get more reliable database for the relation between AE parameters and stress intensity factor, this approach will provide a good information for evaluating both the existence of crack and the minimum detectable size of crack.

• Journal of the Korea institute for structural maintenance and inspection
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• v.24 no.4
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• pp.18-25
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• 2020

The purpose of this study is to investigate the mechanical and carbon-capture properties of foam concrete containing stainless steel argon oxygen decarbonization(AOD) slag. AOD slag was used as a binder, and foam concrete having a foaming ratio of 69 ± 0.5 % and a slurry density of 573.2 to 578.6 kg / ㎥ was produced. In order to examine the effect of carbonation, blended specimen was cured by two types : normal curing and CO₂ curing. As a result of the experiment, the specimens incorporating AOD slag showed higher compressive strength than Plain after CO₂ curing. According to the analysis of the image of foam concrete, it was confirmed that the ST30 has a lower total pore volume and average pore size than plain, resulting in high compressive strength. The SEM analysis confirmed the formation of calcite by carbonation of AOD slag. Through the thermogravimetric analysis, the increase of CO₂ uptake was confirmed by the incorporation of AOD slag. Foam concrete has a higher porosity than normal concrete, so it is expected that carbon-capture performance can be improved by using a AOD slag.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.4
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• pp.399-405
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• 2018

The platelet count in clinical laboratories is essential for the diagnosis and treatment of hemostasis abnormalities, and accurate platelet counting in the low count range is of prime importance for deciding if a platelet transfusion is needed and for monitoring after chemotherapy. Quality control is designed to reduce and correct any deficiencies in the internal analytical process of a clinical laboratory prior to the release of patient results. Fragmented erythrocytes are the major confusing factors for platelet counting because of their similar size to platelets. The authors found that the low range QC values were out of 2SD with a Sysmex automatic analyzer in internal quality control process. Thus far, there has been little discussion on the relationship between hemolysis and the platelet parameters. Therefore, this study focused on the performance of automated platelet counts, including the PLT-F, the PLT-I, and PLT-O methods at the low platelet range using the low level QC materials and compared the 5 platelet parameters with the hemolyzed samples. The results showed that the CV was the smallest with PLT-F and P-LCR increased from 18.4 to 31.9% in the hemolysis samples. These results indicate that a more accurate estimation of the platelet counts can be achieved using the PLT-F method than the PLT-I method at the low platelet range. The use of the PLT-F system improves the confidence of results in low platelets samples in a routine hematology laboratory. The results suggest that P-LCR is a new parameter in assessing samples when the specimen is suspected of hemolysis and deterioration. Nevertheless, further studies will be needed to establish the relationship with P-LCR and hemolysis using human blood specimens.

• Journal of the Korean Geotechnical Society
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• v.24 no.1
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• pp.51-59
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• 2008

In this study, cyclic shear characterics of sand-clay mixtures were analyzed. In order to perform cyclic triaxial tests on sand clay mixtures, natural clays with activity and silica sand were mixed variously to reproduce soils with wide range of grain size compositions. Test specimens with various fines contents were prepared by the moisture compaction and pre-consolidation methods, while paying attention to the void ratio expressed in terms of the sand structure and clay structures, and undrained cyclic shear tests were performed. In the test results, cyclic shear strength decreased with increasing of sand granular void ratio below 20% of fine contents. When the granular void ratio of the test specimen exceeded the maximum void ratio of the silica sand, the clay matrix dominated the soil structure, and soil structures were not influenced by compaction energy. It was observed that, the matrix structure of the coarse particles has great effect on the undrained cyclic shear strength characteristics for sand-clay mixtures, and therefore, it is more appropriate to pay more attention to the density of the sand structure, rather than to the fines content.

• Journal of Korean Society of Steel Construction
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• v.17 no.3 s.76
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• pp.325-335
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• 2005

This study tackles the development of an improved detail of partially restrained CFT square column-to-beam connection and the evaluation of its mechanical behavior under monotonic loading. The connection is designed to strengthen shearing capacity at the bottom of the connection due to the ultimate behavior of PR-CC by its detail of the bottom connection and simplify the fabrication process. The suggested connection is the welded bottom beam flange connection(M-2) and is compared with the existing PR-CC of bolted seat angle connection(M-1). Two specimens were fabricated in actual size and tested under monotonic loading. Based on the test results, the welded bottom beam flange connection exhibited about 85% of the stiffness of steel beam. It was similar to the bolted seat angle connection and behaved as PR-CC. The specimen of the supposed connection type failed at the shear connection of web but was similar to the bolted seat angle connection until the failure. It obtained sufficient stiffness and capacity through the reinforcingsteel and the capacity and deformational ability equivalent to the full-plastic moment through the anchor inside the steel tube at the web connection. So, it can be said that the suggested connection exhibits sufficient ductile behavior.

• Journal of Korean Society of Steel Construction
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• v.29 no.6
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• pp.479-486
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• 2017

Steel beams with corrugated web have been widely used in the steel structures. However, it is challenging to weld the section between the corrugated web and the flange straight, which increases the cost of production. In order to solve this issue, steel beam with intaglio and embossed web (It is called an IEB) was invented. A web with embossment is produced by cold pressing and welded to flange by automatic welding machine. The loading tests were conducted to investigate the load-carrying capacity of IEB, and its test result was compared with that of H-shaped beam having a same size of flange and web. The test results of IEB series showed about 40% higher load capacities than H-shaped series. As a result of comparing the IEB specimen with Eurocodes for steel beams with corrugated web, all of specimens tested in this study did not meet the design value. Therefore, it is difficult to apply existing formula to IEB and new design formula should be presented for field application.

• Journal of the Korean Society for Nondestructive Testing
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• v.33 no.2
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• pp.173-180
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• 2013

Compacted graphite iron(CGI), also known as vermicular graphite iron, is a metal which is gaining popularity in applications that require either greater strength, or lower weight than cast iron. Recently compacted graphite iron has been used for diesel engine blocks. Considering that using in exhaust manifold of the diesel engine, CGI340 was conducted the heat treatment during 1 hour to 96 hours from 873 to 1273 K. Mechanical characteristics were evaluated. The obtained results are as follows; The tensile strength of the heat treated specimens showed overall lower tensile strength than that of the base metal. Tensile strength decreases with increasing of heat treatment time, and the higher heat treatment temperature and the longer time, were more reduced. The fatigue limit by the ultrasonic fatigue test was approximately 130 MPa of base metal, 100 MPa of 1173 K (96 hrs) specimen, respectively. The hardness decreases with increasing heat treatment time, and the higher the heat treatment temperature was lowered hardness distribution. In CGI340, average hardness of nodular graphite was 120 Hv, average hardness of vermicular graphite was 114 Hv. This showed lower hardness than the base structure ferrite. The nodular graphite and vermicular graphite according to the heat treatment temperature and time didn't have a consistent change. However, the grain size of base structure grew with increasing of heat treatment time.

• Composites Research
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• v.29 no.3
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• pp.117-124
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• 2016

The failure strength of composite single-lap joint repaired using scarf patch was investigated by test and finite element method. A total of 45 specimens were tested changing scarf ratio, stacking pattern, and defect size to study the failure strength and mode. Except for one case, all repaired specimens showed the equal or higher strength than the sound specimens and the effect of considered repair parameters was not remarkable. It was found through the failure mode inspection that the surface treatment for bonding was not enough in the case which failed at the lower load than the sound specimen. Three-dimensional finite element analysis was conducted to verify the test results. It was confirmed that the considered repair parameters do not significantly affect the stress distribution of the specimens. It was also observed that the applied tensile load is relieved passing through the overlapped region thickness of which is almost double. From this study, it is concluded that if the bonding procedure for adherends and patch including surface treatment for fabric layer is thoroughly followed, the strength of repaired single-lap joint can be restored up to the strength of sound one.