• 한국발생생물학회지:발생과생식
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• 제14권4호
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• pp.261-268
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• 2010

As in the O. mykiss electrophoretic profiles of RNA, the signals of each RNA sample from 9 individual tissues such as liver, muscle, brain, heart, pituitary gland, kidney, intestine, spleen and gill similar to positive control were obtained. The tissue distributions of the complimentary DNA (cDNA) of O. mykiss four genes were analyzed using quantitative real-time PCR with primer sets for tissue expression analysis. In this rainbow trout species, author obtained bands of various sizes, ranged from 700 bp to 1,400 bp. A dissociation curve was made at the end of each run to make sure that there was no non-specific amplification. Supplementarily, the Ct of each DNA was compared. The Ct values of vinculin with rainbow trout tissues were determined in a manner similar to those for agouti-related protein (AgRP) and melanocortin receptors (MC4R I and MC4R II). Further, obtained Cts for standard curve of each DNA were affected by specific product (vinculin, AgRP and MC4R II genes). After several experiments with four individual genes of rainbow trout, author estimated a variation ratio of the mean Ct value of the DNA extracted using the comparative CTt method was 37.27, and the standard deviation was 5.33. The correlation coefficient between the Ct values and the concentration of cDNA was -0.98, -0.99, -0.91 and -0.86, respectively (vinculin, AgRP, MC4R I and MC4R II genes). Since this correlation showed high linearity, the straight line obtained was used as a standard for the O. mykiss tissues reared in aquarium. A PCR efficiency of 100% is ideally achieved when the slopes are close to the theoretical value of -3.31. According to quantification method, the results of quantification are strongly affected by the DNA fragmentation. The size of most DNA fragments obtained from various tissues of rainbow trout used in the experiment was approximately 100 bp. According to the four slopes, an efficiency of nearly 100% was estimated for four genes detection methods. Additionally, further analysis with more individuals and primers will be required to fully establish optimization in rainbow trout.

• 한국콘텐츠학회논문지
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• 제18권2호
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• pp.450-458
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• 2018

본 연구는 대학의 지식자본이 지역의 기업활동에 미치는 영향을 분석하였다. 그 결과 1단위 대학보유특허 수, 대학의 과학저널 논문 수, 현재 활동기업 수, 지역보유 산업재산권 표준편차의 증가는 신생지식집약기업의 수를 각각 15%, 11%, 54%, 22% 증가시키는 것으로 나타났다. 뿐만 아니라 대학의 지식자본과 지역의 지식자본과의 상호작용을 보여주는 계수도 통계적으로 유의성을 보였다. 한편 지역적 특성을 반영하고 있는 통제 변수 중에서 서울로 부터의 거리를 제외한 지자체의 면적, 인구밀도, 그리고 각 지자체에 살고 있는 20~40대 비중 등은 모두 90% 이상의 통계적 유의성을 보이면서 신생지식집약기업의 창업에 영향을 끼치는 것으로 나타났다. 특히 대학의 지식자본과 지역산업의 지식자본이 동시에 존재함으로서 지식집약기업의 창업에 주는 시너지 효과는 작지만 존재하는 것으로 보이며 두 주체의 소유 지식자본은 지역의 지식집약기업의 창업에는 상호 보완재로서의 역할을 하는 것으로 나타났다.

• 한국측량학회지
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• 제17권2호
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• pp.97-104
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• 1999

수치표고모델(DEM)은 정사영상 제작을 위한 중요한 기초 자료이며, 품질은 대상 점 또는 선형요소의 기하학적 정확도에 의존하게 된다. 따라서 본 연구에서는 격자의 간격과 스캐닝 해상도를 달리하며 상관매칭에 의해 수치표고모델 생성을 시도하였다. 실험결과 추출점의 격자간격이 작을수록 표준편차가 가장 작게 나타났으며 스캐닝의 해상도는 큰 영향이 없는 것으로 나타났다. 또한 수치표고모델에 대한 편이량 분석결과 소축척에 비해 대축척의 항공사진에서 편이가 크게 발생되었으며 이는 폐쇄영역이 가장 큰 영향 요소임을 알 수 있었다. 이러한 패쇄영역을 줄이기 위하여 다축척 영상의 이용과 종 중복도를 증가시키며 실험한 결과 한 쌍의 입체영상보다 다중영상을 이용한 경우 음영 및 폐쇄영역의 크기를 대폭 감소시킬 수 있는 것으로 나타났다.

• 한국산업융합학회 논문집
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• 제23권3호
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• pp.433-444
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• 2020

This study proposes a method to improve the sleep stage and efficiency estimation of sleep apnea patients using a UWB (Ultra-Wideband) radar. Motion and respiration extracted from the radar signal were used. Respiratory signal disturbances by motion artifacts and irregular respiration patterns of sleep apnea patients are compensated for in the preprocessing stage. Preprocessing calculates the standard deviation of the respiration signal for a shift window of 15 seconds to estimate thresholds for compensation and applies it to the breathing signal. The method for estimating the sleep stage is based on the difference in amplitude of two kinds of smoothed respirations signals. In smoothing, the window size is set to 10 seconds and 34 seconds, respectively. The estimated feature was processed by the k-nearest neighbor classifier and the feature filtering model to discriminate between the sleep periods of the rapid eye movement (REM) and non-rapid eye movement (NREM). The feature filtering model reflects the characteristics of the REM sleep that occur continuously and the characteristics that mainly occur in the latter part of this stage. The sleep efficiency is estimated by using the sleep onset time and motion events. Sleep onset time uses estimated features from the gradient changes of the breathing signal. A motion event was applied based on the estimated energy change in the UWB signal. Sleep efficiency and sleep stage accuracy were assessed with polysomnography. The average sleep efficiency and sleep stage accuracy were estimated respectively to be about 96.3% and 88.8% in 18 sleep apnea subjects.

• Geomechanics and Engineering
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• 제3권4호
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• pp.291-321
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• 2011

The paper describes a simple numerical FLAC model that was developed to simulate the dynamic response of two instrumented reduced-scale model reinforced soil walls constructed on a 1-g shaking table. The models were 1 m high by 1.4 m wide by 2.4 m long and were constructed with a uniform size sand backfill, a polymeric geogrid reinforcement material with appropriately scaled stiffness, and a structural full-height rigid panel facing. The wall toe was constructed to simulate a perfectly hinged toe (i.e. toe allowed to rotate only) in one model and an idealized sliding toe (i.e. toe allowed to rotate and slide horizontally) in the other. Physical and numerical models were subjected to the same stepped amplitude sinusoidal base acceleration record. The material properties of the component materials (e.g. backfill and reinforcement) were determined from independent laboratory testing (reinforcement) and by back-fitting results of a numerical FLAC model for direct shear box testing to the corresponding physical test results. A simple elastic-plastic model with Mohr-Coulomb failure criterion for the sand was judged to give satisfactory agreement with measured wall results. The numerical results are also compared to closed-form solutions for reinforcement loads. In most cases predicted and closed-form solutions fall within the accuracy of measured loads based on ${\pm}1$ standard deviation applied to physical measurements. The paper summarizes important lessons learned and implications to the seismic design and performance of geosynthetic reinforced soil walls.

• Steel and Composite Structures
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• 제26권4호
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• pp.485-499
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• 2018

In this paper, a hollow steel tube (HST) shear connector is proposed for use in a slim-floor system. The HST welded to a perforated steel beam web and embedded in concrete slab. A total of 10 push-out tests were conducted under static loading to investigate the mechanical behavior of the proposed HST connector. The variables were the shapes (circular, square and rectangular) and sizes of hollow steel tubes, and the compressive strength of the concrete. The failure mode was recorded as: concrete slab compressive failure under the steel tube and concrete tensile splitting failure, where no failure occurred in the HST. Test results show that the square shape HST in filled via concrete strength 40 MPa carried the highest shear load value, showing three times more than the reference specimens. It also recorded less slip behavior, and less compressive failure mode in concrete underneath the square hollow connector in comparison with the circular and rectangular HST connectors in both concrete strengths. The rectangular HST shows a 20% higher shear resistance with a longer width in the load direction in comparison with that in the smaller dimension. The energy absorption capacity values showed 23% and 18% improvements with the square HST rather than a headed shear stud when embedded in concrete strengths of 25 MPa and 40 MPa, respectively. Moreover, an analytical method was proposed and predicts the shear resistance of the HST shear connectors with a standard deviation of 0.14 considering the shape and size of the connectors.

• 한국멀티미디어학회논문지
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• 제16권12호
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• pp.1376-1384
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• 2013

본 논문에서는 산업용 CT 볼륨데이터에서 템플릿 매칭을 통하여 제품의 이물질을 자동 검출하는 기법을 제안한다. 제안방법은 다음과 같은 세 단계로 이루어진다. 첫째, 다운 샘플링 데이터에서 잡음제거 후 제품을 배경과 분리하고, 영상의 평균값 및 표준편차를 이용하여 초기 이물질 후보를 추출한 후, K-평균 클러스터링을 이용하여 이물질 후보를 추출한다. 둘째, 템플릿 매칭을 이용하여 제품과 유사도가 다른 이물질을 검출한다. 이 때, 검출하고자 하는 이물질의 크기에 따라 밝기값평균차이(SSD)와 조인트 엔트로피를 이용한 유사도 평가를 통하여 이물질을 검출한다. 셋째, 원 볼륨데이터에서 이물질 검출률을 개선하기 위하여 여과기법으로 제품의 이물질을 최종 검출한다. 본 제안 방법의 결과를 평가하기 위해 산업용 CT 볼륨데이터와 시뮬레이션 데이터를 사용하여 육안평가, 정확성 평가와 수행시간 측정을 수행하였다. 정확성 평가를 위하여 기존 밝기값 기반 검출 기법을 비교방법으로 사용하고, 다이스 계수 유사도를 측정하였다.

• 약학회지
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• 제54권4호
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• pp.295-299
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• 2010

The aim of this study was to develop and validate for determination of alibendol in human plasma by HPLC method. After precipitation of 500 ${\mu}l$ plasma samples by 50% methanol 50 ${\mu}l$ and 60% perchloric acid 30 ${\mu}l$ and the supernatant 50 ${\mu}l$ was injected into HPLC. The assay was performed isocratically using 10 mM potassium phosphate (pH 3.0) and acetonitrile (80 : 20, v/v) as mobile phase. The $C_{18}$ column (particle size $3.5{\mu}m$, $4.6{\times}50$ mm, Zorbax Eclipse) was used as a solid phase. The mobile phase was delivered at a flow-rate of 1.7 ml/min, detection was by ultraviolet absorption at 232 nm and concentrations were calculated on the basis of peak areas. In these conditions, alibendol can be separated from ethylparaben, the internal standard, and endogenous substances. The retention times of alibendol and ethylparaben were just about 2.6 and 3.5 minutes, respectively. This rapid HPLC method was validated by examining the precision and accuracy for inter- and intra-day analysis. The standard curve was linear ($R^2$=1.0000) over the concentration range of 0.05~20 ${\mu}g$/ml. The inter-day relative standard deviation (R.S.D.) and accuracy were 0.2~12.2% and 94.4~101.2% (82.7% at the lower limit of quatitation). The intra-day R.S.D. and accuracy were 0.1~11.8% and 98.8~102.5%, respectively. The method was successfully applied to the determination of alibendol in plasma for a pharmacokinetic study.

• 터널과지하공간
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• 제14권5호
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• pp.363-372
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• 2004

구마모토 안산암을 대상으로 이중 비틀림(DT) 시험을 통해 유도 홈의 단면 형상이 암석의 응력부식지수에 미치는 영향에 대하여 조사하였다. 정변위속도법에서 변위속도가 증가할수록 균열성장속도는 증가하지만 파괴인성계수는 평균 2.07 MN/m$^{3}$2/로 거의 일정하였다. 정변위법을 통하여 유도 홈의 형상이 사각형, 원형, 삼각형인 시험편을 이용하여 얻은 응력부식지수는 각각 평균 37, 36, 38로 홈의 형상에 관계없이 거의 일정한 값을 보이지만 표준편차는 삼각형 홈에서 가장 크게 나타났다. 암석의 DT시험은 중앙부에 유도 홈이 있는 시험편을 이용하는 것이 효과적이지만 광물의 평균입경 이상의 폭을 가진 사각형 단면의 홈을 이용하는 것이 가장 적절하다.

• 대한치과교정학회지
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• 제21권1호
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• pp.131-170
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• 1991

This study was designed to analyze morphological characteristics of Korean young adults, norms and standard deviation of variables, sexual differences, correlationship between each area of face and correlationship between hard tissue and soft tissue. The primary sample consisted of 45 males and 57 females who were early and middle twenties and had acceptable profile, no history of previous orthodontic treatment, absence of remarkably large overjet and overbite, full complement of permanent teeth, Class I skeletal and dental relationships and good vertical facial proportions. Their cephalograms were analyzed morphologically with a computer morphometrics. Then the final sample - 25 males and 38 females - were selected within 1 S.D. of E-line, ANB, P/A facial height ratio, Interincisal angle, L1 to A-Pog, ODI and APDI. The results of the study were as follows: 1 In the form and proportion of facial skeleton there were no significant differences between males and females, but in the size males were larger than females. 2. The dental protrusion patterns had no significant sexual difference and no significant correlationship between protrusion of upper lip and inclination of upper incisor. But mentolabial angle had positively correlated with interincisal angle and negatively with inclination of upper and lower incisor. 3. In the relationship between nose and soft-tissue profile, males were larger than females in nasal length, height and angular measurements. 4. In analysis of soft-tissue profile, males were larger than females in the length and thickness. In the angular measurements and proportion of soft-tissue profile, there were no significant differences between males and females.