• Preventive Nutrition and Food Science
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• v.15 no.2
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• pp.159-166
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• 2010

The aim of this study was to develop species-specific primer sets for kimchi Lactobacillus. Known gene sequences of Lactobacillus 16S rRNA were collected from the NCBI Gene bank, and 69 primer sets were designed using the homologous gene sequence. Six species of kimchi Lactobacilli were used as reference strains: Lactobacillus brevis KCTC3102, Lactobacillus farciminis KCTC3681, Lactobacillus fermentum KCTC3112, Lactobacillus hilgardii KCTC3500, Lactobacillus plantarum KCTC3099, and Lactobacillus sanfranciscensis KCTC3205. PCR amplification and gel electrophoresis were performed to identify the accuracy and specificity of the developed primer set. The results show that the primer set of 5'-aagcctgcgaaggcaag-3' & 5'-aggccaccggctttg-3', 5'-acatactatgcaaatctaagagattagacg-3' & 5'-actgagaatggctttaagagattagcttac-3' resulted in a specific PCR band on L. hilgardii, and primer set of 5'-ctaataccgcataacaactactttcacat-3' & 5'-aacttaataaaccgcctacattctctttac-3' on L. farciminis. The results indicate that the developed primer sets can provide a useful tool for the identification and differentiation of L. hilgardii and L. farciminis from other Lactobacillus species of kimchi.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.6
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• pp.788-798
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• 2017

Antarctic krill Euphausia superba are important components of the Antarctic marine ecosystem both economically and ecologically; to manage this species effectively, their distribution and abundance must be understood. Using the Kwang Ja-Ho (3,012 tonnage), a commercial fishing vessel, we conducted acoustic surveys during April 13-24, 2016, to estimate the distribution and population size of krill around the South Shetland Islands of the Antarctic Continent, We used acoustic techniques based on the dB-difference, a method used mainly to classify of marine species. We found that Antarctic krill were present in numbers over 99% at six survey stations, with the exception of Station 3, where we only found Electrona carlsbergi. There was no difference in cell size due to frequency differences, but echo signals differed between species: 4.7-12.0 dB for Antarctic krill, and -4.1~0 dB for Electrona carlsbergi.

• Journal of Wetlands Research
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• v.7 no.4
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• pp.51-65
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• 2005

The insects and Benthic Macro-Invertebrates are very important for index of investigation. This reserch is executed a field investigation with a date analysis of the laboratory under such as above hypothesis and we have grasped the change of the creature according to the season through the executing of field investigation(We executed six times about the insects, seven times about the Benthic Macro-Inbertebrates each expending from Apr. to Oct. 2004). The result of summary in 2004 show that, we have grasped 12 orders 56 familys 116 species on 2001 and 10 orders 42 familys 78 species on 2002 through the investigation. Also we have grasped 13 orders 53 familys 114 species on 2003 and 12 orders 63 familys 121 speceies on 2004 through the investigation. In the case of enthic Macro-Invertebrates, we executed the analysis through the investigation result expending from 2003 to 2004 due to there is no literature investigation record from 2001 to 2002. We have grasped 8 orders 17 familys 24 species on 2003 and 7 orders 11 familys 24 species on Aug. 2004 through the investigation. We executed the analysis about an each condition due to it is difficult to compare the above result simply by the various variable at the investigation. We expect that it become the fundamental reference data to establish a efficient mode of life undo plan after grasped the inhabitation and movement route of creature inside the river through this reserch.

• Korean Journal of Environment and Ecology
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• v.18 no.1
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• pp.1-6
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• 2004

Seed characteristics-weight, seed length and width, pure seed percentage are measured on 15 native herb species, and seeds of six native herb species were tested on the seedbed in the forest with autumn seeding. Seeds of 13 species including Aster scaber were very small; seed length is less than 7mm and seed width is less than 3mm pure seed percentages of 10 native herb speciesin-cluding Ligularia fischeriwere were much lower than 5％. Germination percent of five native herb species were significantly different among the growing sites. The mean germination percent was highest 47.00％ for Angelica gigas, 38.25％ for Ligularia fischeri, 30.88％ for PimPinella brachycarpa, 15.75％ for Aralia continetalis, 10.00％ for Cirsium setidens, and the lowest 9.32％ for Aster scaber.

• The Korean Journal of Zoology
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• v.11 no.2
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• pp.65-68
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• 1968

Collections of the drosophilid flies were made at 6 regions near the dimilitarized znoe form October 1966 to June 1968, with particular attention to their habitats and distribution. A total of 5022 flies representing 34 species belonging to 7 genera were obtained mostly by the traps baited with fermenting apples and banana, partially by the aid of net. The data accumulated for about 21 months are summarized in this paper. Drosophila auraria and D. angularis were found to be most common species showing the most extensive distribution. In eastern part, a total of 1552 individuals(30.90%) representing 27 species, 7 genera were sampled. In central part, a total of 2658 flies (52.93%) yielded 29 species, 6 genera. In western part, a total of 812 specimens(16.17%) which belong to 16 species representing 3 genera were collected. Most specimens belonging to the genus Scaptomyza have been found on grasses.

• The Korean Journal of Zoology
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• v.35 no.2
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• pp.219-225
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• 1992

The genetic variation in mitochondrial DNA (mtDNA) was analysed within and between two species of tree frogs. Hyla japonica and H. suweonensis from South Korea. Purified mtDNAs were digested with each of 11 restriction enLvmes which cleave at six base recognition sequences. The genome size of H. iaponica revealed ho types (20.0 $\pm$ 0.3 and 19.6 $\pm$ 0.3 kb) and this difference is explained by either addition or deletion of about 0.4 kb fragment. On the other hand, the genome sire of H. suueonensis was about 19.0 $\pm$ 0.4 kb only. For the analysis, level of fragment homology (F) and nucleotide sequence divergence (p) were estimated from comparisons of digestion profiles. Among four populations of H. iaponica, substantial mean sequence divergence was 0.017 (range 0.001-0.026); between identical types, 0.001 IslilaRl type) and 0.004 (Large type) respectively; between different ones, 0.024 (range 0.023-0.026). The level of sequence divergence between he species was 0.142 (range 0.131-0.146). This result suggested that he species ㅂwere distinctly differentiated species. The divergence time between ko species was estimated 7.1 million years.

• Molecules and Cells
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• v.43 no.8
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• pp.728-738
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• 2020

Time and cost-effective production of next-generation sequencing data has enabled the performance of population-scale comparative and evolutionary studies for various species, which are essential for obtaining the comprehensive insight into molecular mechanisms underlying species- or breed-specific traits. In this study, the evolutionary and functional analysis of Korean native pig (KNP) was performed using single nucleotide polymorphism (SNP) data by comparative and population genomic approaches with six different mammalian species and five pig breeds. We examined the evolutionary history of KNP SNPs, and the specific genes of KNP based on the uniqueness of non-synonymous SNPs among the used species and pig breeds. We discovered the evolutionary trajectory of KNP SNPs within the used mammalian species as well as pig breeds. We also found olfaction-associated functions that have been characterized and diversified during evolution, and quantitative trait loci associated with the unique traits of KNP. Our study provides new insight into the evolution of KNP and serves as a good example for a better understanding of domestic animals in terms of evolution and domestication using the combined approaches of comparative and population genomics.

• Korean Journal of Medicinal Crop Science
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• v.13 no.2
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• pp.69-74
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• 2005

Kalopanax pictus is a long-lived woody species mostly distributed in East Asia. K. pictus has been regarded as medically and ecologically important species in Korea. Thornless castor aralia variant, local name 'Cheongsong' is an endemic to Cheongsong province in Korea. Random amplified polymorphic DNA (RAPD) was used to investigate the genetic variation and structure of Korean populations of two species. A high level of genetic variation was found in six K. pictus populations. Twelve primers revealed 49 loci, of which 29 were polymorphic (59.2%). Nei's gene diversity for K.pictus and K. pictus variant were 0.119 and 0.098, respectively. Mean of genetic diversity in K. pictus was higher than average values for species with similar life history traits. The asexual and sexual reproduction, perennial habitat, and longevity are proposed as possible factors contributing to high genetic diversity. An indirect estimate of the number of migrants per generation (Nm=0.857) indicated that gene flow was not extensive among Korean populations of K.pictus. It is suggested that the isolation of geographical distance and reproductive isolation between K.pictus and K.pictus variant populations may have played roles in shaping the population structure of this species.

• Korean journal of applied entomology
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• v.30 no.1
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• pp.80-85
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• 1991

For the study of ant fauna in Mt. Kyeryongsan, we selected three areas and collected 317 colonies nine times from 1986 to 1990. The communities of ants were composed of 3 subfamilies, 23 genera and 45 species. Among them, Solenopsis sp. was an unrecorded species in Korea. The number of ant species distributed in each area was almost the same, and the species distributed uniformly in the nine subareas were Paratrechina flavipes and Aphaenogaster japonica. The number of species collected in the only one subarea were ten, and six among them were collected in the area of Sinwonsa temple, the westward slope. It is remarkable that Camponotus tokioensis, which is known to be distributed only in the island and coast so far, is found to be distributed in the area of Sinwonsa temple as well.

• Korean Journal of Ichthyology
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• v.18 no.3
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• pp.244-250
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• 2006

The ichthyofauna and fish community structure were investigated at five stations in Yeongok stream, Gangneung, during April and December 2005 monthly. During the surveyed period twenty nine species belonging to ten families were collected. Dominant species in number was Zacco platypus (relative abundance : 29.0%) and subdominant was Rhynchocypris steindachneri (12.1%). There were six Korean endemic species including Rhynchocypris kumgangensis. Koreocobitis rotundicaudata and Luciogobius guttatus that were not reported in near streams were collected in Yeongok stream only. Ladislabia taczanowskii was considered as a geographically important species. There were stabilized fish community structure with species diversity index of the community 2.521 in Yeongok stream ichthyofauna.