• Journal of Information Technology Applications and Management
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• v.19 no.3
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• pp.69-84
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• 2012

A web site focused on a specific product brand so called product-brand web site has become one major marketing tool as such product-brand web site provides chances for customers to experience the detailed information of the specific brand as well as emphatic the image of the brand. The purpose of this research is to examine the influence of the product-brand web site on the formation of attitude toward the product brand. Subjects of this study are users of two web sites of leading sanitary product brands. The result shows that not only emotion, playfulness and convenience perceived from the web site but also existing attitude toward the brand influence the attitude toward the web site. Further, the attitude toward the web site in turn affects attitude toward the image of the brand. The importance of this study is in its conceptualization and empirical verification of the relationship between the product-brand web site and the reputation of a brand.

• 한국전자현미경학회:학술대회논문집
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• 2001.11a
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• pp.18-22
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• 2001

A technique to characterize specific site of materials using a combination of a dedicated focused ion beam system(FIB), and Intermediate-voltage scanning transmission electron microscope(STEM) or transmission electron microscope(TEM) equipped with a scanning electron microscope(SEM) unit has been developed. The FIB system is used for preparation of electron transparent thin samples, while STEM or TEM is used for localization of a specific site to be milled in the FIB system. An FIB-STEM(TEM) compatible sample holder has been developed to facilitate thin sample preparation with high positional accuracy Positional accuracy of $0.1{\mu}m$ or better can be achieved by the technique. In addition, an FIB micro-sampling technique has been developed to extract a small sample directly from a bulk sample in a FIB system These newly developed techniques were applied for the analysis of specific failure in Si devices and also for characterization of a specific precipitate In a metal sample.

• Microbiology and Biotechnology Letters
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• v.26 no.5
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• pp.387-392
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• 1998

Brevibacterium lactofermentum, a gram-positive bacteria, has both the diaminopimelate (DAP) pathway and meso-DAP-dehydrogenase (DDH) pathway for L-lysine biosynthesis. To investigate importance of DDH pathway and the related ddh gene in lysine production, we introduced site-specific mutagenesis technique. A 300 bp DNA fragment central to the meso-DAP-dehydrogenase gene (ddh) of B. lactofermentum was used to inactive chromosomal ddh gene via homologous recombination. Southern hybridization analysis confirmed that the chromosomal ddh gene was disrupted by the vector sequence. The B. lactofementum ddh mutant obtained have an inactive DDH pathway. The results reveal that inactivation of the ddh gene in B. lactofermentum leads to dramatic reduction of lysine production as well as decrease of the growth rate, indicating that the DDH pathway is essential for high-level lysine production as well as biosynthesis of meso-DAP.

• Journal of Soil and Groundwater Environment
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• v.26 no.6
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• pp.74-81
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• 2021

A simple and efficient scheme is presented that attempts to implement the site-specific denitrification rate in the reactive transport modeling for the nitrate in groundwater. A series of correlation analyses were conducted using 133 datasets obtained from different nitrate-contaminated sites to find the empirical relationships between denitrification rates and various subsurface properties. Based on Pearson's correlation analysis, the soil organic carbon concentrations showed a statistically significant correlation (r = 0.75, p < 0.05) with the denitrification rates. A linear regression was performed, which could be utilized to effectively determine the site-specific denitrification rate based on the soil organic carbon concentration of a site. The proposed method is expected to effectively replace the conventional methods which either were too complicated for practical application or impose large uncertainties that might end up with unreliable results.

• Journal of the Earthquake Engineering Society of Korea
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• v.3 no.4
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• pp.1-10
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• 1999

Free-field ground motion during earthquake is significantly affected by the local site conditions and it is essential for the seismic design to perform the site specific ground response analysis. In this paper, the procedures of site specific ground response analysis were suggested based on the Korean seismic guideline and the review of state of the art technologies. The concept of ground response analysis was introduced, and the techniques of obtaining soil data for one dimensional equivalent linear analysis which include site investigation planning, field and laboratory testing techniques, deformational characteristics of soils at small to large strains, and site characterization techniques combining field and laboratory test results, were suggested. Finally, the case study was performed at Inchon area following the suggested procedure.

• Microbiology and Biotechnology Letters
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• v.39 no.4
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• pp.337-344
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• 2011

Integrase MJ1 from the bacteriophage ${\Phi}FC1$ carries out recombination between two DNA sequences (the phage attachment site, attP and the bacterial attachment site, attB) in NIH3T3 mouse cells. In this study, the integration vector containing attP, attB and the integrase gene MJ, was constructed. The integration mediated by integrase MJ1 in Escherichia coli led to excision of LacZ. Therefore, the frequency of integration was measured by the counting of the white colony, which is detectable on X-Gal plates. The extrachromosomal integration in NIH3T3 mouse cells was monitored by the expression of the green fluorescent protein (GFP) as a reporter. To demonstrate integration mediated integrase MJ1 in NIH3T3 cells, vectors containing attP and attB were co-transfected into NIH3T3 cells. The integration was confirmed by fluorescent microscopy. The expression of GFP was induced in NIH3T3 cells expressing MJ1 without accessory factors. By contrast, the excision mediated by the MJ1 between attR and attL had no effect on the expression of GFP. These results suggest that integrase MJ1 may enable a variety of genomic modifications for research and therapeutic purposes in higher living cells.

• Earthquakes and Structures
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• v.14 no.4
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• pp.285-295
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• 2018

In this study, the effect of local site conditions (site class and site amplifications) and structural variability are investigated on fragility functions of typical building structures. The study area is chosen as Eastern Turkey. The fragility functions are developed using site-specific uniform hazard spectrum (UHS). The site-specific UHS is obtained based on simulated ground motions. The implementation of ground motion simulation into seismic hazard assessment has the advantage of investigating detailed local site effects. The typical residential buildings in Erzincan are represented by equivalent single degree of freedom systems (ESDOFs). Predictive equations are accomplished for structural seismic demands of ESDOFs to derive fragility functions in a straightforward manner. To study the sensitivity of fragility curves to site class, two sites on soft and stiff soil are taken into account. Two alternative site amplification functions known as generic and theoretical site amplifications are examined for these two sites. The reinforced concrete frames located on soft soil display larger fragilities than those on stiff soil. Theoretical site amplification mostly leads to larger fragilities than generic site amplification more evidently for reinforced concrete buildings. Additionally, structural variability of ESDOFs is generally observed to increase the fragility especially for rigid structural models.

• Journal of Soil and Groundwater Environment
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• v.25 no.3
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• pp.65-73
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• 2020

Exposure assessment methodology for outdoor air inhalation pathways (i.e., inhalation of volatile compounds and fugitive dust in outdoor air) was investigated. Default values of several parameters currently used in Korea (e.g., Q/C; inverse value of concentration per unit flux, and frs; soil fraction in PM10) may not be suitable and lack site-specificity, as they have been adopted from the risk assessment guidance of the United States or the Netherlands. Such limitation can be addressed to a certain degree by incorporating the volatilization factor (VF) and the particulate emission factor (PEF) with Box model. This approach was applied to an exposure assessment of a site contaminated with petroleum hydrocarbons in Korea. The result indicated that the suggested methodology led to more accurate site-specific exposure assessment for outdoor inhalation pathways. Further work to establish methodology to determine site-specific Q/C values in Korea needs to be done to secure the reliability of the exposure assessment for outdoor air inhalation pathways.

• Trans-
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• v.12
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• pp.223-244
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• 2022

The rapid change of the culture and art environment is led to new art & cultural education and differences in culture and art education facilities, away from the traditional closed space culture and art education. Phenomena such as plays out of the theater, exhibitions out of the art museum, and pictures taking a walk indicate that cultural and artistic educational facilities can no longer stay in the existing paradigm and are changing along with the changes in the cultural and creative world. Therefore, to develop a site-specific theater education program centered around a specific place rather than a theater or studio, in line with the changing times of cultural and artistic educational facilities, this researcher analyzes Brecht's radio play experiment and the recent performative performance experiment. Furthermore, using the regional and community values of arts and culture education confirmed as the motive for research on site-specific theater programs, I analyze the implementation and application process by experimenting with theater programs out of the theaters and studios. As a prior study, research on site-specific performances is being conducted relatively actively, but earlier studies were dealing only with the Ligna group performance cases are lacking. However, I would like to use the previous research on site-specific performance cases as an epistemological background. As a result of the study, for the place-specific theater program through a total of 10 learners, a text based on a specific place was created that did not depend on traditional literary texts. Through this, the possibility of a site-specific theater education program could be confirmed.

• Korean Journal of Microbiology
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• v.29 no.4
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• pp.209-214
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• 1991

The PRD1 DNA polymerase is a small multi-functional enzyme containing conserved amino acid sequences shared by family B DNA polymerases. Thus the PRD1 DNA polymerase provides an useful model system with which to study structure-functional relationships of DNA polymerase molecules. In order to investigate the functional and structural roles of the highly conserved amino acid sequences, we have introduced three mutations into a conserved amino acid of the PRD1 DNA polymerase. Genetic complememtation study indicated that each mutation inactivated DNA polymerase catalytic activity.