• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2002년도 추계학술대회
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• pp.159-161
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• 2002

Bacteriophage lambda integrase carries out the site-specific recombination of lambda. Integrase contains two DNA binding domains with distinct sequence specificity, namely arm-type binding and core-type binding domains. The amino-terminal arm-binding domain is structurally related to the three-stranded $\beta-sheet$ family of DNA-binding domains. Integrase binding to the high affinity arm-type site by the amino-terminal domain facilitates Int binding to the low affinity core-type site, where the cleavage and strand exchange occurs. The amino-terminal domain of Int also modulates the core-binding and catalysis through intramolecular domain-domain interaction and/or intermolecular interactions between Int monomers. In addition, the amino-terminal domain interacts cooperatively with excisionase during excision. This indicates that amino-terminal domain of Int plays an important role in formation of proper higher-order nucleoprotein structure required for lambda site-specific recombination.

• 한국방재학회 논문집
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• 제7권5호
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• pp.139-150
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• 2007

컴퓨터 기술이 급속히 발전됨에 따라, 최근에는 많은 분야에서 지리정보시스템(GIS)이 활용되고 있다. 이 연구에서는 서울 내의 한 시범 지역을 대상으로 지하 지반 조건에 따라 영향을 받는 부지 효과에 관련된 지진피해 가능성을 GIS 기법을 이용하여 평가하였다. 시범 지역에서의 지역적 부지고유 지진 응답의 평가를 위하여 서울 대도시 지역에 대한 기존 시추 조사 자료의 분포를 파악하였다. GIS 토대의 지반 정보 시스템(GTIS)을 구축하여 시범 지역 전체에 대한 공간 지층 정보를 예측하였다 서울 시법 지역의 부지고유 지진응답 예측을 위한 부지 주기$(T_G)$의 정밀구역화를 GTIS 내에서 수행하였다. 공간 분포 정밀구역화 지도로부터 시범 지역 내 2 층에서 4 층 건물들의 지진 취약성을 확인하였다. 또한, GTIS 내에서 $T_G$ 분포를 토대로 설계 지반 운동 결정을 위한 지반 분류 지도를 작성하였으며, 그로부터 대상 시범 지역 부지의 거의 대부분이 현행 지반 분류 C 와 D로 분류됨을 확인하였다. 이와 같은 서울 시범 지역에 대한 지진 정밀구역화 적용 구조 체계는 특별히 기존 시추 자료를 활용하여 대도시 서울 지역 전체에 대해 적용 가능할 것으로 판단된다.

• Bulletin of the Korean Chemical Society
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• 제32권12호
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• pp.4427-4430
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• 2011

• Bulletin of the Korean Chemical Society
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• 제28권6호
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• pp.907-908
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• 2007

• BMB Reports
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• 제31권4호
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• pp.399-404
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• 1998

In order to gain further insight on the relationship between structure and function of glutathione S-transferase (GST), the six active-site mutants, R13T, K44T, Q51A, Q64A, S65A, and D98A, of human GST P1-1 were expressed in Escherichia coli and purified to electrophoretic homogeneity by affinity chromatography on immobilized GSH. The active-site mutants showed marked differences in substrate specificity. The substitution of Gln51 with threonine resulted in a drastic decrease in the specific activities to <10% of the wild-type value. The substitution of Arg13 with threonine resulted in more decreased specific activity toward cumene hydroperoxide and in the $I_{50}$ values of S-(2,4-dinitrophenyl) glutathione and benanstatin A. These results suggest that the substitution of Arg13 with threonine changes the conformation of the active site to increase the affinity for the product or electrophilic substrate. Lys44 seems to be in the vicinity of the H-site of hGST P1-1 or may contribute to some extents to the electrophile binding.

• Journal of Microbiology and Biotechnology
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• 제19권3호
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• pp.217-228
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• 2009

Mobile genetic segments, or transposons, are also referred to as jumping genes as they can shift from one position in the genome to another, thus inducing a chromosomal mutation. According to the target site-specificity of the transposon during a transposition event, the result is either the insertion of a gene of interest at a specific chromosomal site, or the creation of knockout mutants. The former situation includes the integration of conjugative transposons via site-specific recombination, several transposons preferring a target site of a conserved AT-rich sequence, and Tn7 being site-specifically inserted at attTn7, the downstream of the essential glmS gene. The latter situation is exploited for random mutagenesis in many prokaryotes, including IS (insertion sequence) elements, mariner, Mu, Tn3 derivatives (Tn4430 and Tn917), Tn5, modified Tn7, Tn10, Tn552, and Ty1, enabling a variety of genetic manipulations. Randomly inserted transposons have been previously employed for a variety of applications such as genetic footprinting, gene transcriptional and translational fusion, signature-tagged mutagenesis (STM), DNA or cDNA sequencing, transposon site hybridization (TraSH), and scanning linker mutagenesis (SLM). Therefore, transposon-mediated genetic engineering is a valuable discipline for the study of bacterial physiology and pathogenesis in living hosts.

• 한국지진공학회논문집
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• 제4권2호
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• pp.37-46
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• 2000

본 논문에서는 부지특성을 고려한 설계지반운동의 산정방법을 연구하였으며 그 해석결과를 지반 구조물의 내진설계에 적용하는 방법을 제안하였다 지진응답 해석시 사용되는 설계응답스펙트럼과 설계시간 이력등의 입력운동의 통제점 위치가 지반구조물 내진설동 지층내 암반운동 그리고 노두운동을 사용하는 방법으로 나눌 수 있고 이에 따라 작용 설계지진운동이 변화하므로 지반구조물의 경계조건에 적합한 방법을 사용하여야 한다.

• 국제학술발표논문집
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• The 6th International Conference on Construction Engineering and Project Management
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• pp.109-110
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• 2015

This paper presents a computational method that identifies an exact set of optimal overlap rates between critical activities to meet job site specific needs by using rework cost-slope. The procedures to compute the exact solution are provided in peudocode algorithm. The method is coded into Exact Concurrent Construction Scheduling system that allows practitioners to make more informed decision in accordance with the site-specific condition involved in the overlapping of critical activities. Test cases verify the validity of the computational method and the usability of the system.

• Steel and Composite Structures
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• 제26권2호
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• pp.197-211
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• 2018

The study presents the earthquake performance of the Bosphorus Bridge under multi-point earthquake excitation considering the spatially varying site-specific earthquake motions. The elaborate FE model of the bridge is firstly established depending on the new considerations of the used FEM software specifications, such as cable-sag effect, rigid link and gap elements. The modal analysis showed that singular modes of the deck and the tower were relatively effective in the dynamic behavior of the bridge due to higher total mass participation mass ratio of 80%. The parameters and requirements to be considered in simulation process are determined to generate the spatially varying site-specific ground motions. Total number of twelve simulated ground motions are defined for the multi-support earthquake analysis (Mp-sup). In order to easily implement multi-point earthquake excitation to the bridge, the practice-oriented procedure is summarized. The results demonstrated that the Mp-sup led to high increase in sectional forces of the critical components of the bridge, especially tower base section and tensile force of the main and back stay cables. A close relationship between the dynamic response and the behavior of the bridge under the Mp-sup was also obtained. Consequently, the outcomes from this study underscored the importance of the utilization of the multi-point earthquake analysis and the necessity of considering specifically generated earthquake motions for suspension bridges.

• Tuberculosis and Respiratory Diseases
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• 제48권6호
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• pp.879-886
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• 2000

연구배경: 폐특이 전사조절 유전자인 Thyroid Transcription Factor-1 (TTF-1)유전자는 폐에 선택적인 유전자의 표현의 조절에 중요한 전사인자로 작용하고 폐의 발생에서 morphogenic protein으로서 작용한다. 그러나 현재까지 이 TTF-1 유전자의 전사인자에 대한 연구는 거의 미미하다. DNase 1 hypersensitive(DH) regions은 활동적인 염색체에 대한 중요한 표식자이며 유전자를 조절하는 많은 DNA sequences와 밀접한 관계가 있다. 방법 : 추정적인 distal regulatory elements를 밝혀 내기 위해서 TTF-1을 표현하는 인간의 폐선암 세포주인 NCI-H441을 사용해 DNase 1 hypersensitive site assay를 이용하였다. 결과 : TTF-1 유전자에는 전사의 시작부위에서 +150, -450, -800, 그리고 -1500 base pair부위에 4곳의 DH sites가 있음을 할 수 있었다. 결론 : 이상의 결과로 전사 조절부위가 TTF-1 유전자 내에 그리고 5' prime부위에 위치함을 추정할 수 있었다.