• Asian Pacific Journal of Cancer Prevention
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• v.15 no.22
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• pp.9557-9565
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• 2014

MiRNAs are endogenous, single stranded ~22-nucleotide non-coding RNAs (ncRNAs) which are transcribed by RNA polymerase II and mediate negative post-transcriptional gene regulation through binding to 3'untranslated regions (UTR), possibly open reading frames (ORFs) or 5'UTRs of target mRNAs. MiRNAs are involved in the normal physiology of eukaryotic cells, so dysregulation may be associated with diseases like cancer, and neurodegenerative, heart and other disorders. Among all cancers, lung cancer, with high incidence and mortality worldwide, is classified into two main groups: non-small cell lung cancer and small cell lung cancer. Recent promising studies suggest that gene expression profiles and miRNA signatures could be a useful step in a noninvasive, low-cost and repeatable screening process of lung cancer. Similarly, every stage of lung development during fetal life is associated with specific miRNAs. Since lung development and lung cancer phenomena share the same physiological, biological and molecular processes like cell proliferation, development and shared mRNA or expression regulation pathways, and according to data adopted from various studies, they may have partially shared miRNA signature. Thus, focusing on lung cancer in relation to lung development in miRNA studies might provide clues for lung cancer diagnosis and prognosis.

• Journal of Microbiology and Biotechnology
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• v.25 no.10
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• pp.1714-1727
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• 2015

This work describes a new strategy for optimal design of Multiplex-PCR primer sequences. The process is based on the Particle Swarm Optimization-Simplex algorithm (Mult-PSOS). Diverging from previous solutions centered on heuristic tools, the Mult-PSOS is selfconfigured because it does not require the definition of the algorithm's initial search parameters. The successful performance of this method was validated in vitro using Multiplex-PCR assays. For this validation, seven gene sequences of the most prevalent bacteria implicated in urinary tract infections were taken as DNA targets. The in vitro tests confirmed the good performance of the Mult-PSOS, with respect to infectious disease diagnosis, in the rapid and efficient selection of the optimal oligonucleotide sequences for Multiplex-PCRs. The predicted sequences allowed the adequate amplification of all amplicons in a single step (with the correct amount of DNA template and primers), reducing significantly the need for trial and error experiments. In addition, owing to its independence from the initial selection of the heuristic constants, the Mult-PSOS can be employed by non-expert users in computational techniques or in primer design problems.

• Journal of Microbiology and Biotechnology
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• v.23 no.9
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• pp.1244-1252
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• 2013

Sugar beet pulp is an abundant industrial waste material that holds a great potential for bioethanol production owing to its high content of cellulose, hemicelluloses, and pectin. Its structural and chemical robustness limits the yield of fermentable sugars obtained by hydrolyzation and represents the main bottleneck for bioethanol production. Physical (ultrasound and thermal) pretreatment methods were tested and combined with enzymatic hydrolysis by cellulase and pectinase to evaluate the most efficient strategy. The optimized hydrolysis process was combined with a fermentation step using a Saccharomyces cerevisiae strain for ethanol production in a single-tank bioreactor. Optimal sugar beet pulp conversion was achieved at a concentration of 60 g/l (39% of dry weight) and a bioreactor stirrer speed of 960 rpm. The maximum ethanol yield was 0.1 g ethanol/g of dry weight (0.25 g ethanol/g total sugar content), the efficiency of ethanol production was 49%, and the productivity of the bioprocess was 0.29 $g/l{\cdot}h$, respectively.

• Journal of the Korean Ceramic Society
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• v.47 no.4
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• pp.333-337
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• 2010

Domain structure and polarization switching in (001)-oriented $Pb(Mg_{1/3}Nb_{2/3})O_3-x%PbTiO_3$ (PMN-x%PT) crystals for x=20 and 35at% have been investigated in-situ by scanning force microscope (SFM) in a piezoresponse mode under a step DC electrical voltage. In the initial annealed condition, polar nano domains (PND) and domain striations oriented along {110} were observed in x=20 and x=35, respectively. For x=20, domain switching occurs by heterogeneous nucleation, where nucleation is not confined in the vicinity of domain boundaries, but rather can occur throughout the crystal volume. However, for x=35, domain switching tends to be preferentially initiated near pre-existing twin boundaries. With increasing the applying voltage, the nuclei density increased and assembled into {110} striations, indicating a stress-accommodated domain growth process. At higher voltage, nucleation occurs heterogeneously throughout the crystal volume.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.21 no.3
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• pp.222-226
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• 2008

With different seed adhesion methods, we obtained two different aspects with or without micro-bubble in the interface between a seed and a dense graphite seed holder. To improve the quality of SiC wafer, we introduced a sucrose caramelizing step at the seed adhesion using the sucrose, The n-type 2 inch single crystal exhibiting the polytype of 6H-SiC were successfully fabricated and carrier concentration levels of about $10^{16}/cm^3$ was determined from Hall measurements, As compared to the characteristics of SiC crystal grown with micro-bubble in the interface between the seed and the dense graphite seed holder, the SiC crystal grown without micro-bubble definitely exhibited lower resistivity, lower micropipe density and higher mobility relatively.

• Proceedings of the KSRS Conference
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• 1998.09a
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• pp.186-191
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• 1998

The first step in change detection in any SAR monitoring, including SAR interferometry, is the co-registration of the images. CCPs (Ground Control Points) for co-registration are usually detected manually, but for qualitative analyses of enormous volumes of data, some automation of the process will become necessary. An automated determination of common CCPs for the same path/row data is especially desirable. We selected the intersections of linear features as the candidates of common GCPs Very bright point targets, which are commonly used as GCPs, have the drawback of appearing and disappearing depending on the conditions of the observation. But in the case of linear features, some detailed elements may appear differently in some case, but the overall line-likeness will remain. In this study, we selected 18 common GCPs for a single-look JERS-1 SAR image of Omaezaki area in central Japan. Although the GCPs in the first image had to be selected either interactively or semi-automatically, the same GCPs in all other images were successively detected automatically using a tiny sub-image around each GCP and a dilated mask of each linear feature in the first image as the reference data.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2002.05a
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• pp.77-81
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• 2002

A new method of optimal blank shape design using the initial nodal velocity (INOV) has been proposed for the drawings of arbitrary shaped cups. With the given information of tool shape and the final product shape, corresponding initial blank shape has been found from the motion of boundary nodes. Although the sensitivity method, the past work of Hynbo Shim and Kichan Son, has been proved to be excellent method to find optimal blank shapes, the method has a problem that a couple of deformation analysis is required at each design step and it also exhibits an abnormal behaviors in the rigid body rotation prevailing region. In the present method INOV, only a single deformation analysis per each design stage is required. Drawings of practical products as well as oil-pan have been chosen as the examples. At every case the optimal blank shapes have been obtained only after a few times of modification without predetermined deformation path. The deformed shape with predicted optimal blank almost coincides with the target shape at every case. Through the investigation the INOV is found to be very effective in the arbitrary shaped drawing process design.

• Journal of Internet Computing and Services
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• v.20 no.6
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• pp.29-36
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• 2019

When dealing with outdoor images in a variety of computer vision applications, the presence of shadow degrades performance. In order to understand the information occluded by shadow, it is essential to remove the shadow. To solve this problem, in many studies, involves a two-step process of shadow detection and removal. However, the field of shadow detection based on CNN has greatly improved, but the field of shadow removal has been difficult because it needs to be restored after removing the shadow. In this paper, it is assumed that shadow is detected, and shadow-less image is generated by using original image and shadow mask. In previous methods, based on CGAN, the image created by the generator was learned from only the aspect of the image patch in the adversarial learning through the discriminator. In the contrast, we propose a novel method using a discriminator that judges both the whole image and the local patch at the same time. We not only use the residual generator to produce high quality images, but we also use joint loss, which combines reconstruction loss and GAN loss for training stability. To evaluate our approach, we used an ISTD datasets consisting of a single image. The images generated by our approach show sharp and restored detailed information compared to previous methods.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.15 no.12
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• pp.1054-1059
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• 2002

A great deal of research has been done in the field of characterization for piezoelectric thin films after the first report on the measurement for the piezoelectric coefficient of thin films in 1990. The main idea of this research is to provide a distinctive solution for the measurement and standardization of both the longitudinal and the transverse piezoelectric d-coefficients, d33 and d31, of ferroelectric thin films. In general, to get these two coefficients of thin films, two different measuring systems are required. Here, we propose the improved method for the evaluation of these two coefficients with single equipment and with the relatively convenient procedure. The two-step loading process of applying the both positive and the negative pressure has been introduced to acquire the piezoelectric coefficients. These results have been calibrated for both the longitudinal and 4he transverse piezoelectric d-coefficients, d33 and 431, of thin films by comparison with the virtual standard created from FEM. In this experiments, we have obtained d33 of 331pC/N and 031 of -92.2pC/N for the PZT thin films.

• Proceedings of the KIEE Conference
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• 2001.11b
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• pp.395-398
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• 2001

Recently, the increasing need for internet and the activation of hiring business of communication line makes the demand for OPGW cable which can construct the optical communication network in the basis of existing overhead power transmission line. Especially, the demand is focused on the high fiber count OPGW due to high capacity transmission. In step with the trend toward high fiber count cable we have developed the Stainless Steel Loose Tube type OPGW within which have 144core firstly in KOREA. This paper describes the cable design and manufacturing process which gives the stable operation in very severe conditions and the long-term reliability test results conducted in according to dominant specification IEEE Std. 1138-1194.