• Title/Summary/Keyword: Single-Cell Analysis

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Characterization and Preparation of Ceramic Interconnect of SOFC by Thermal Plasma Spray Coating Process (열 플라스마 용사법에 의해 코팅된 고체산화물 연료전지용 세라믹 연결재 특성 연구)

  • Park, Kwang-Yeon;Lim, Tak-Hyoung;Lee, Seung-Bok;Park, Seok-Joo;Shin, Dong-Ryul;Song, Rak-Hyun
    • 한국신재생에너지학회:학술대회논문집
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    • 2009.11a
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    • pp.187-190
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    • 2009
  • $LaCrO_3$ series are the most common candidate materials for a ceramic interconnect for SOFC and the thermal expansion coefficient of these matches very well with that of YSZ electrolyte. In this study, characteristics of the coated films on the anode-support, which were formed by using $La_{0.8}Ca_{0.2}CrO_3$, $La_{0.8}Sr_{0.2}CrO_3$, $La_{0.8}Ca_{0.2}Co_{0.1}Cr_{0.9}O_3$ for ceramic interconnet for SOFC, were investigated. All powders showed single perovskite phase and the precursors had spherical shapes of $2{\mu}m{\sim}30{\mu}m$. According to SEM analysis, coated film of LCC on pretreated anode-support was more thicker, whereas the coated film on untreated anode-support was densely formed. As the results of electrical conductivity of anode-support coated with the ceramic interconnects, LCCC exhibited the most excellent electrical conductivity of 0.15S/cm at $750^{\circ}C$.

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Spatial Distribution Mapping of Cyanobacteria in Daecheong Reservoir Using the Satellite Imagery (위성영상을 이용한 대청호 남조류의 공간 분포 맵핑)

  • Back, Shin Cheol;Park, Jin Ki;Park, Jong Hwa
    • Journal of The Korean Society of Agricultural Engineers
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    • v.58 no.2
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    • pp.53-63
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    • 2016
  • Monitoring of cyanobacteria bloom in reservoir systems is important for water managers responsible of water supply system. Cyanobacteria affect the taste and smell of water and pose considerable filtration problems at water use places. Harmful cyanobacteria bloom in reservoir have significant economic impacts. We develop a new method for estimating the cyanobacteria bloom using Landsat TM and ETM+ data. Developed model was calibrated and cross-validated with existing in situ measurements from Daecheong Reservoir's Water Quality Monitoring Program and Algae Alarm System. Measurements data of three stations taken from 2004 to 2012 were matched with radiometrically converted reflectance data from the Landsat TM and ETM+ sensor. Stepwise multiple linear regression was used to select wavelengths in the Landsat TM and ETM+ bands 1, 2 and 4 that were most significant for predicting cyanobacteria cell number and bio-volume. Based on statistical analysis, the linear models were that included visible band ratios slightly outperformed single band models. The final monitoring models captured the extents of cyanobacteria blooms throughout the 2004-2012 study period. The results serve as an added broad area monitoring tool for water resource managers and present new insight into the initiation and propagation of cyanobacteria blooms in Daecheong reservoir.

Uncertainty Analysis and Improvement of an Altitude TestFacility for Small Jet Engines

  • Jun, Yong-Min;Yang, In-Young;Kim, Chun-Taek;Yang, Soo-Seok;Lee, Dae-Sung
    • International Journal of Aeronautical and Space Sciences
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    • v.5 no.1
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    • pp.46-56
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    • 2004
  • The verification and improvement of the measurement uncertainty have beenperformed in the altitude test facility for small gas turbine engines, which was built atthe Korea Aerospace Research Institute (KARI) in October 1999. This test is performedwith a single spool turbojet engine at several flight conditions. This paper discussesthe evaluation and validation process for the measurement uncertainty improvements usedin the altitude test facility. The evaluation process, defined as tests before the facilitymodification, shows that the major contnbutors to the measurement uncertainty are theflow meter discharge coefficient, the inlet static and total pressures, the cell pressureand the fuel flow rate. The measurement uncertainty is focused on the primary parametersof the engine performance such as airflow rate, thrust and specific fuel consumption (SFC).The validation process, defined as tests after the facility modification, shows that themeasurement uncertainty, in seal level condition, is tmproved to the acceptable level throughthe facility modification. In altitude test conditions, the measurement uncertainties arenot improved as much as the uncertainty in sea level condition.

Expressional Subpopulation of Cancers Determined by G64, a Co-regulated Module

  • Min, Jae-Woong;Choi, Sun Shim
    • Genomics & Informatics
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    • v.13 no.4
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    • pp.132-136
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    • 2015
  • Studies of cancer heterogeneity have received considerable attention recently, because the presence or absence of resistant sub-clones may determine whether or not certain therapeutic treatments are effective. Previously, we have reported G64, a co-regulated gene module composed of 64 different genes, can differentiate tumor intra- or inter-subpopulations in lung adenocarcinomas (LADCs). Here, we investigated whether the G64 module genes were also expressed distinctively in different subpopulations of other cancers. RNA sequencing-based transcriptome data derived from 22 cancers, except LADC, were downloaded from The Cancer Genome Atlas (TCGA). Interestingly, the 22 cancers also expressed the G64 genes in a correlated manner, as observed previously in an LADC study. Considering that gene expression levels were continuous among different tumor samples, tumor subpopulations were investigated using extreme expressional ranges of G64-i.e., tumor subpopulation with the lowest 15% of G64 expression, tumor subpopulation with the highest 15% of G64 expression, and tumor subpopulation with intermediate expression. In each of the 22 cancers, we examined whether patient survival was different among the three different subgroups and found that G64 could differentiate tumor subpopulations in six other cancers, including sarcoma, kidney, brain, liver, and esophageal cancers.

Capacity of Multiuser Diversity with Cooperative Relaying in Wireless Networks (협동 릴레이와 다중 사용자 다이버시티를 이용하는 무선 통신 네트워크의 용량 분석)

  • Joung, Hee-Jin;Mun, Cheol;Seo, Jeong-Tae;Yoo, Kang-Hee
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.33 no.6C
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    • pp.423-428
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    • 2008
  • We consider the use of cooperative diversity in a multiuser wireless data network. This paper provides an analysis of the interaction between cooperative diversity and multiuser diversity on downlink channels. By using approximation of the signal-to-noise ratio (SNR) distribution of each cooperative diversity link by Gamma distribution, an analytic expression is derived for the average throughput of a single-cell wireless system with multiple cooperative diversity links combined with a fair-access scheduler. The proposed analytic approach is verified through comparisons with simulated results and shows that cooperative diversity makes the detrimental impacts on multiuser diversity.

Circuit Modeling and Analysis of Touch Screen Panel (터치스크린 패널의 회로 모델링 및 분석)

  • Byun, Kisik;Min, Byung-Wook
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.25 no.1
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    • pp.47-52
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    • 2014
  • A simple RC circuit model of large-scale touch screen panels is developed and the frequency range of the RC model is analyzed. 2D EM simulation results of a single touch cell are cascaded for a 23 inch touch panel using a circuit simulator, and the shortest and longest channels of the full panel are modeled with a 5-element RC circuit. The 5-element RC circuit can model the touch screen panel upto 130 kHz with the channel phase error of $10^{\circ}$. 7-element RC circuit model is also proposed and the frequency range for the channel phase error of $10^{\circ}$ is extended to 200 kHz.

Agrobacterium-Mediated Transformation of Flammulina velutipes with NaOH Treatment (NaOH처리에 의한 Agrobacterium이용 팽이균사체 형질전환)

  • Shin, Dong-Il;Park, Hee-Sung
    • The Korean Journal of Mycology
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    • v.39 no.3
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    • pp.235-238
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    • 2011
  • Agrobacterium harboring vector pCHBs with hygromycin phosphotransferase(hph) and hepatitis B virus surface antigen(HBsAg)gene was transformed into the mycelial culture of Flammulina velutipes. In particular, mild NaOH solution was treated to the mycelia before Agrobacterium infection step. This was purposed to generate putative surface wounds in the mycelial cell walls. The results showed that hygromycin-resistant($hyg^r$) mycelia could be obtained only from NaOH-treated mycelia but not from intact mycelia. The integration of $hyg^r$ gene in fungal genome was confirmed by PCR. In addition, a single transgene integration and heterologous protein expression in F. velutipes could be verified by Southern blot hybridization and western blot analysis, respectively. This study demonstrated an efficient tool for the Agrobacterium-mediated transformation of F. velutipes mycelia.

Construction of a live attenuated Salmonella strain expressing FanC protein to prevent bovine enterotoxigenic Escherichia coli and evaluation of its immunogenicity in mice

  • Won, Gayeon;Kim, Hee Jung;Lee, John Hwa
    • Korean Journal of Veterinary Research
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    • v.57 no.1
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    • pp.9-15
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    • 2017
  • To construct a novel vaccine candidate against bovine enterotoxigenic Escherichia coli (ETEC), FanC, the major subunit of K99 fimbriae adhesion, was inserted into secretion plasmid pYA3560 containing a ${\beta}-lactamase$ secretion system. This was then transformed into ${\Delta}asd$ ${\Delta}crp$ Salmonella (S.) Typhimurium and designated as JOL950. Secretion of recombinant fanC fimbrial antigens was confirmed by immunoblot analysis. Groups of mice were inoculated with single or double doses of JOL950. Another group was used as a negative control. Compared to control mice, all immunized mice had significantly higher levels (p < 0.05) of serum immunoglobulin (Ig)G, and secretory IgA against FanC. The IgG2a and IgG1 titer assays revealed that immunization highly induced IgG2a compared to that of IgG1, indicating that T helper-1- related cell-mediated immune responses may be elicited by JOL950. The results show that both systemic and mucosal immunities against selected fimbrial antigens of bovine ETEC expressed by a live attenuated S. Typhimurium strain are prominently produced in mice immunized with JOL950 via an oral route.

Low temperature wet-chemical synthesis of spherical hydroxyapatite nanoparticles and their in situ cytotoxicity study

  • Mondal, Sudip;Dey, Apurba;Pal, Umapada
    • Advances in nano research
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    • v.4 no.4
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    • pp.295-307
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    • 2016
  • The present research work reports a low temperature ($40^{\circ}C$) chemical precipitation technique for synthesizing hydroxyapatite (HAp) nanoparticles of spherical morphology through a simple reaction of calcium nitrate tetrahydrate and di-ammonium hydrogen phosphate at pH 11. The crystallinity of the single-phase nanoparticles could be improved by calcinating at $600^{\circ}C$ in air. Thermogravimetric and differential thermal analysis (TG-DTA) revealed the synthesized HAp is stable up to $1200^{\circ}C$. Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) studies confirmed the formation of spherical nanoparticles with average size of $23.15{\pm}2.56nm$ and Ca/P ratio of 1.70. Brunauer-Emmett-Teller (BET) isotherm of the nanoparticles revealed their porous structure with average pore size of about 24.47 nm and average surface area of $78.4m2g^{-1}$. Fourier transform infrared spectroscopy (FTIR) was used to confirm the formation of P-O, OH, C-O chemical bonds. Cytotoxicity and MTT assay on MG63 osteogenic cell lines revealed nontoxic bioactive nature of the synthesized HAp nanoparticles.

Anti-Inflammatory Effects of Ethyl Acetate Fraction from Cnidium officinale Makino on LPS-Stimulated RAW 264.7 and THP-1 Cells

  • Jeong, Jin-Boo;Hong, Se-Chul;Jeong, Hyung-Jin;Koo, Jin-Suk
    • Korean Journal of Plant Resources
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    • v.25 no.3
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    • pp.299-307
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    • 2012
  • This work aimed to elucidate the anti-inflammatory effects of ethyl acetate fraction from Cnidium officinale Makino with a cellular system of LPS-stimulated RAW 264.7 and THP-1 cells. Some key pro-inflammatory cytokines and mediators including NO, iNOS, $PGE_2$, COX-2, TNF-${\alpha}$, NF-${\kappa}B$ p50 and NF-${\kappa}B$ p65 were studied by sandwich ELISA and western blot analysis. Ethyl acetate fraction could significantly inhibit the production of NO, $PGE_2$, TNF-${\alpha}$, iNOS and COX-2 in LPS-stimulated cell than that of single LPS-stimulated. And ethyl acetate fraction suppresses the activation of NF-${\kappa}B$ p50 and NF-${\kappa}B$ p65. All the results showed that ethyl acetate fraction had a good anti-inflammatory effect on LPS-stimulated RAW264.7 and THP-1 cells. Taken together, the anti-inflammatory actions of ethyl acetate fraction from Cnidium officinale Makino might be due to the down-regulation of NO, $PGE_2$, TNF-${\alpha}$, iNOS and COX-2 via the suppression of NF-${\kappa}B$ activation.