• 제목/요약/키워드: Single-Cell Analysis

Search Result 860, Processing Time 0.032 seconds

Estimation of Fermentation State and Metabolic Stoichiometry of Kyuywomyces marxianus (Krupwomyces marxianus의 발효상태 및 대사 양론식 추정)

  • 류두현
    • KSBB Journal
    • /
    • v.8 no.3
    • /
    • pp.272-281
    • /
    • 1993
  • State varibles were estimated for fermentations of K. marxianus under various dilution rates and dissolved oxygen concentrations. The number of elementary reaction stoichiometry with fixed coefficients was determined by singular variable decomposition. Stoichiometry with feasible physical meaning was obtained by target factor analysis. States of fermentations were estimated by linear quadratic programming. The process conditions of single cell production to maximize carbon source consumption were suggested.

  • PDF

Performance Improvement of A Hybrid TDMA/CDMA Systems with Multi-channel Linear Equalizer (다중채널 선형등화기를 이용한 혼합 TDMA/CDMA 시스템의 성능개선)

  • 김응배
    • The Journal of Korean Institute of Communications and Information Sciences
    • /
    • v.25 no.9A
    • /
    • pp.1273-1281
    • /
    • 2000
  • In this paper we studied for multi-user detection system, which hold the merit of CDMA system and can enhance the system capacity. We designed actually realizable quasi-optimal multiuser detection system by use of linear equalizer on the concept that multiuser detection algorithm can be reduced by combining TDMA with CDMA. we call this the hybrid TDMA/CDMA system. And we proposed multiuser detection system, which can use PSAD and MSDD channel estimation method. As a result of performance analysis we acquired equal or much better performance by use of linear multichannel equalizer in the case of not so many user. And on the occasion of many user within cell we can also acquired much better performance in comparison with conventional single user detection system by use of hybrid TDMA/CDMA system.

  • PDF

Enhanced Equivalent Circuit Modeling for Li-ion Battery Using Recursive Parameter Correction

  • Ko, Sung-Tae;Ahn, Jung-Hoon;Lee, Byoung Kuk
    • Journal of Electrical Engineering and Technology
    • /
    • v.13 no.3
    • /
    • pp.1147-1155
    • /
    • 2018
  • This paper presents an improved method to determine the internal parameters for improving accuracy of a lithium ion battery equivalent circuit model. Conventional methods for the parameter estimation directly using the curve fitting results generate the phenomenon to be incorrect due to the influence of the internal capacitive impedance. To solve this phenomenon, simple correction procedure with transient state analysis is proposed and added to the parameter estimation method. Furthermore, conventional dynamic equation for correction is enhanced with advanced RC impedance dynamic equation so that the proposed modeling results describe the battery dynamic characteristics more exactly. The improved accuracy of the battery model by the proposed modeling method is verified by single cell experiments compared to the other type of models.

Organic Photovoltaic Effects Depending on the Layer Thickness (CuPc/$C_{60}$를 이용한 유기 광기전 소자에서 유기층의 두께에 따른 특성)

  • Han, Wone-Keun
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
    • /
    • 2005.07a
    • /
    • pp.535-536
    • /
    • 2005
  • Organic photovoltaic effects were studied in a device structure of ITO/CuPc/Al and ITO/CuPc/$C_{60}$/BCP/Al. A thickness of CuPc layer was varied from 10 nm to 50 nm, we have obtained that the optimum CuPc layer thickness is around 40 nm from the analysis of the current density-voltage characteristics in CuPc single layer photovoltaic cell. From the thickness-dependent photovoltaic effects in CuPc/$C_{60}$ heterojunction devices, higher power conversion efficiency was obtained in ITO/20nm CuPc/40nm $C_{60}$/Al, which has a thickness ratio (CuPc:$C_{60}$) of 1:2 rather than 1:1 or 1:3. Light intensity on the device was measured by calibrated Si-photodiode and radiometer/photometer of International Light Inc(IL14004).

  • PDF

Application of Grid-based Kinematic Wave Storm Runoff Model

  • Kim, Seong-Joon;Kim, Sun-Joo;Chae, Hyo-Seok
    • Proceedings of the Korea Water Resources Association Conference
    • /
    • 2000.05a
    • /
    • pp.20-27
    • /
    • 2000
  • The grid-based KlneMatic wave STOrm Runoff Modei (Kim, 1998; Kim, et al., 1998) which predicts temporal variation and spatial distribution of saturated overland flow, subsurface flow and stream flow was evaluated at two watersheds. This model adopts the single overland flowpath algorithm and simulates surface and/or subsurface water depth at each cell by using water balance of hydrologic components. The model programed by C-language uses ASCII-formatted map data supported by the irregular gridded map of the GRASS (Geographic Resources Analysis Support System) GIS and generates the spatial distribution maps of discharge, flow depth and soil moisture of the watershed.

  • PDF

Dynamic Network Loading Model based on Moving Cell Theory (Moving Cell Theory를 이용한 동적 교통망 부하 모형의 개발)

  • 김현명
    • Journal of Korean Society of Transportation
    • /
    • v.20 no.5
    • /
    • pp.113-130
    • /
    • 2002
  • In this paper, we developed DNL(Dynamic Network Loading) model based on Moving cell theory to analyze the dynamic characteristics of traffic flow in congested network. In this paper vehicles entered into link at same interval would construct one cell, and the cells moved according to Cell following rule. In the past researches relating to DNL model a continuous single link is separated into two sections such as running section and queuing section to describe physical queue so that various dynamic states generated in real link are only simplified by running and queuing state. However, the approach has some difficulties in simulating various dynamic flow characteristics. To overcome these problems, we present Moving cell theory which is developed by combining Car following theory and Lagrangian method mainly using for the analysis of air pollutants dispersion. In Moving cell theory platoons are represented by cells and each cell is processed by Cell following theory. This type of simulation model is firstly presented by Cremer et al(1999). However they did not develop merging and diverging model because their model was applied to basic freeway section. Moreover they set the number of vehicles which can be included in one cell in one interval so this formulation cant apply to signalized intersection in urban network. To solve these difficulties we develop new approach using Moving cell theory and simulate traffic flow dynamics continuously by movement and state transition of the cells. The developed model are played on simple network including merging and diverging section and it shows improved abilities to describe flow dynamics comparing past DNL models.

Characterization of Oszinc626, knock-out in zinc finger RING-H2 protein gene, in Ac/Ds mutant lines of rice(Oryza sativar L.) (Zinc finger RING-H2 protein관련 Ac/Ds전이인자 삽입 변이체 Oszinc626 유전자의 특성 분석)

  • Park, Seul-Ah;Jung, Yu-Jin;Ahn, Byung-Ohg;Yun, Doh-Won;Ji, Hyeon-So;Park, Yong-Hwan;Eun, Moo-Young;Suh, Seok-Cheol;Lee, Soon-Youl;Lee, Myung-Chul
    • Journal of Plant Biotechnology
    • /
    • v.35 no.3
    • /
    • pp.177-183
    • /
    • 2008
  • Ac/Ds mutant lines of this study were transgenic rice plants, each of which harbored the maize transposable element Ds together with a GUS coding sequence under the control of a promoterless(Ds-GUS). We selected the mutants that were GUS expressed lines, because the GUS positive lines will be useful for identifying gene function in rice. One of these mutants was identified knock-out at Oszinc626(NP_001049991) gene, encoding a RING-H2 zinc-finger protein, by Ds insertion. In this mutant, while primary root development is normal, secondary root development from lateral root was very poor and seed development was incomplete compare with normal plant. RING zinc-finger proteins play important roles in the regulation of development in a variety of organisms. In the plant kingdom, a few genes encoding RING zinc-finger proteins have been documented with visible effects on plant growth and development. The consensus of the RING-H2(C3-H2-C3 type) domain for this group of protein is $Cys-X_2-Cys-X_{28}-Cys-X-His-X_2-His-X_2-Cys-X_{14}-Cys-X_2-Cys$. Oszinc626 encodes a predicted protein product of 445 amino acids residues with a molecular mass of 49 kDa, with a RING-zinc-finger motif located at the extreme end of the C-terminus. RT-PCR analysis indicated that the expression of Oszinc626 gene was induced by IAA, cold, dehydration, high-salinity and abscisic acid, but not by 2,4-D, and the transcription of Oszinc626 gene accumulated primarily in rice immature seeds, root meristem and shoots. The gene accumulation patterns were corresponded with GUS expression.

Synthesis and Characterization of Pt based Alloy Catalysts for Direct Ethanol Fuel Cell (직접 에탄올 연료전지용 백금합금촉매의 합성과 특성분석)

  • Kim, Yi-Young;Kim, Soo-Kil;Han, Jong-Hee;Kim, Han-Sung
    • Journal of the Korean Electrochemical Society
    • /
    • v.11 no.2
    • /
    • pp.109-114
    • /
    • 2008
  • Though ethanol can theoretically generate 12 electrons during oxidation to carbon dioxide, the complete oxidation of ethanol is hard to achieve due to the strong bond between the two carbons in its molecular structure. Therefore, development of high activity catalyst for ethanol oxidation is necessary for the commercialization of direct ethanol fuel cell. In this study, some binary and ternary electrocatalysts of PtSn/C and PtSnAu/C have been synthesized and characterized. The catalysts were fabricated with modified polyol method with the amounts of 20 wt%, where the Pt : Sn ratios in the PtSn/C were 1 : 0, 4 : 1, 3 : 1, 2 : 1, 1.5 : 1, 1 : 1, 1 : 1.5 and Pt:Sn:Au ratios in the PtSnAu/C were 5 : 5 : 0, 5 : 4 : 1, 5 : 3 : 2, 5 : 2 : 3. From the XRD and TEM analysis results, the catalysts were found to have face centered cubic structure with particle size of around $1.9{\sim}2.4\;nm$. The activity in the ethanol oxidation was examined with cyclic voltammetry and the results indicated that PtSn(1.5 : 1)/C and PtSnAu(5 : 2 : 3)/C had the highest activity in each catalyst system. Further tests with single cell were performed with those catalysts. It was found that PtSn/C(1.5 : 1) exhibited the best performance while the long term stability of PtSnAu/C(5 : 2 : 3) is better than PtSn/C(1.5 : 1).

Overexpressed Derlin-1 Inhibits ER Expansion in the Endothelial Cells Derived from Human Hepatic Cavernous Hemangioma

  • Hu, Dong;Ran, Yu-Liang;Zhong, Xing;Hu, Hai;Yu, Long;Lou, Jin-Ning;Sun, Li-Xing;Yang, Zhi-Hua
    • BMB Reports
    • /
    • v.39 no.6
    • /
    • pp.677-685
    • /
    • 2006
  • Proteins that are unfolded or misfolded in the endoplasmic reticulum (ER) must be targeted for refolding or degradation to maintain the homeostasis of the ER. Derlin-1 was reportedly implicated in the retro-translocation of misfolded proteins from the ER to the cytosol for degradation. In this report, we showed that Derlin-1 was down-regulated in the endothelial cells derived from human hepatic cavernous hemangioma (CHEC) compared with other tested cells. Electron microscopy analysis showed that ER was aberrantly enlarged in CHEC cells, but not in other tested cells. When overexpressed, Derlin-1 induced the dilated ER to return normal size. This ER dynamic was associated with the activation of unfolded protein response (UPR). In CHEC cells where Derlin-1 was down-regulated, increased expression of the immunoglobulin heavy chain-binding protein (Bip) and UPR-specific splicing of X-box DNA-binding protein 1 (XBP1) mRNA were detected, as compared with that in other tested cells, indicating that UPR was activated. After Derlin-1 overexpression, the extent of UPR activation diminished, as evidenced by decreased expression of Bip, reduced amount of the spliced form of XBP1 ($XBP1_S$), and elevated expression of the unspliced form of XBP1 ($XBP1_U$). Taken together, these findings provide another example of a single protein being able to affect ER dynamic in mammalian cells, and an insight into the possible molecular mechanism(s).

Determination of the linear elastic stiffness and hygroexpansion of softwood by a multilayered unit cell using poromechanics

  • Gloimuller, Stefan;de Borst, Karin;Bader, Thomas K.;Eberhardsteiner, Josef
    • Interaction and multiscale mechanics
    • /
    • v.5 no.3
    • /
    • pp.229-265
    • /
    • 2012
  • Hygroexpansion of wood is a known and undesired characteristic in civil engineering. When wood is exposed to changing environmental humidity, it adsorbs or desorbs moisture and warps. The resulting distortions or - at restrained conditions - cracks are a major concern in timber engineering. We herein present a multiscale model for prediction of the macroscopic hygroexpansion behavior of individual pieces of softwood from their microstructure, demonstrated for spruce. By applying poromicromechanics, we establish a link between the swelling pressure, driving the hygroexpansion of wood at the nanoscale, and the resulting macroscopic dimensional changes. The model comprises six homogenization steps, which are performed by means of continuum micromechanics, the unit cell method and laminate theory, all formulated in a poromechanical framework. Model predictions for elastic properties of wood as functions of the moisture content closely approach corresponding experimental data. As for the hygroexpansion behavior, the swelling pressure has to be back-calculated from macroscopic hygroexpansion data. The good reproduction of the anisotropy of wood hygroexpansion, based on only a single scalar calibration parameter, underlines the suitability of the model. The multiscale model constitutes a valuable tool for studying the effect of microstructural features on the macroscopic behavior and for assessing the hygroexpansion behavior at smaller length scales, which are inaccessible to experiments. The model predictions deliver input parameters for the analysis of timber at the structural scale, therewith enabling to optimize the use of timber and to prevent moisture-induced damage or failure.