• Imaging Science in Dentistry
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• v.30 no.4
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• pp.275-279
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• 2000

Purpose: Radiation damage is produced and viable cell number is reduced. We need to know the type of cell death by the ionizing radiation and the amount and duration of cell cycle arrest. In this study, we want to identified the main cause of the cellular damage in the oral cancer cells and normal keratinocytes with clinically useful radiation dosage. Materials and Methods: Human gingival tissue specimens obtained from healthy volunteers were used for primary culture of the normal human oral keratinocytes (NHOK). Primary NHOK were prepared from separated epithelial tissue and maintained in keratinocyte growth medium containing 0.15 mM calcium and a supplementary growth factor bullet kit. Fadu and Hep-2 cell lines were obtained from KCLB. Cells were irradiated in a /sup 137/Cs γ-irradiator at the dose of 10 Gy. The dose rate was 5.38 Gy/min. The necrotic cell death was examined with Lactate Dehydrogenase (LDH) activity in the culture medium. Every 4 day after irradiation, LDH activities were read and compared control group. Cell cycle phase distribution and preG1-incidence after radiation were analyzed by flow cytometry using Propidium Iodine staining. Cell cycle analysis were carried out with a FAC Star plus flowcytometry (FACS, Becton Dickinson, USA) and DNA histograms were processed with CELLFIT software (Becton Dickinson, USA). Results: LDH activity increased in all of the experimental cells by the times. This pattern could be seen in the non-irradiated cells, and there was no difference between the non-irradiated cells and irradiated cells. We detected an induction of apoptosis after irradiation with a single dose of 10 Gy. The maximal rate of apoptosis ranged from 4.0% to 8.0% 4 days after irradiation. In all experimental cells, we detected G2/M arrest after irradiation with a single dose of 10 Gy. Yet there were differences in the number of G2/M arrested cells. The maximal rate of the G2/M ranges from 60.0% to 80.0% 24h after irradiation. There is no significant changes on the rate of the G0/G1 phase. Conclusion: Radiation sensitivity was not related with necrosis but cell cycle arrest and apoptosis. These data suggested that more arrested cell is correlated with more apoptosis.

• 한국신재생에너지학회:학술대회논문집
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• 2010.11a
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• pp.78.1-78.1
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• 2010

The metal-supported solid oxide fuel cell (SOFC) was studied. Hydrocarbon fueled operation is necessary to make SOFC system. Different operating characteristics for metal-supported SOFC are used than for conventional ones as hydrocarbon fueled operation. Metal-supported SOFC was successfully fabricated by a high temperature sinter-joining method and the cathode was in-situ sintered. Synthetic gas, which is compounded as the diesel reformate gas composition and low hydrocarbons was completely removed by the diesel reformer. Metal-supported SOFC with synthetic gas was operated and evaluated and its characteristics analyzed. Button cell and $5{\times}5cm^2$ single stack were mainly operated and analyzed. Long-term operation using diesel reformate shows degradation, and degradation analysis was completed in the view of metal oxidation. Solution to increase stability of long-term operation was tried in the way of materials and operating conditions. Finally, $5{\times}5cm^2$ metal-supported single stack using synthetic gas was operated for 1000 hours under the modified condition.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.19 no.7
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• pp.601-604
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• 2006

In this paper, we propose the single poly-Si Flash EEPROM device with a new structure which does not need the high voltage switching circuits. The device was designed, fabricated and characterized. From the measurement results, it was found that the program, the erase and the read operations worked properly. The threshold voltage was 3.1 V after the program in which the control gate and the drain were biased with 12 V and 7 V for $100{\mu}S$, respectively. And it was 0.4 V after the erase in which the control gate was grounded and the drain were biased with 11 V for $200{\mu}S$. On the other hand, it was found that the program and the erase speeds were significantly dependent on the capacitive coupling ratio between the control gate and the floating gate. The larger the capacitive coupling ratio, the higher the speeds, but the target the area per cell. The optimum structure of the cell should be chosen with the consideration of the trade-offs.

• Journal of computational fluids engineering
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• v.6 no.1
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• pp.1-13
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• 2001

The natural convection heat transfer and solidification in a stratified pool are studied. The flow and heat transfer characteristics in a heat generating pool are compared between single-layered and double-layered pools. And local Nusselt number distributions on outer walls are obtained to consider thermal loads on a vessel wall. The cooling and solidification of Al₂O₃/Fe melt in a hemispherical vessel are simulated to study the mechanism of heat transfer and temperature distribution. A unstructured mesh is chosen for this study because of the non-orthogonality originated from the boundaries of double-layered pool. Interface between the layers is modeled to be fixed. With this assumption mass flux across the interface is neglected, but shear force and heat flux are considered by boundary conditions. The colocated cell-centered finite volume method is used with the Rhie-Chow interpolation to compute cell face velocity. To prevent non-physical solutions near walls in case body force is large the wall pressure is extrapolated by the way to include body force. The numerical solutions calculated by current method show that averaged downward heat flux of the double-layered pool increases compared to single-layered pool and maximum temperature occurs right below the interface of the layers.

• Environmental Mutagens and Carcinogens
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• v.22 no.2
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• pp.83-89
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• 2002

The single cell gel electrophoresis (comet) assay is one of the useful tools for the study of genetic damage in humans exposed to environmental mutagens and carcinogens. This study was undertaken to evaluate the status of DNA damage in peripheral lymphocytes depending on their sex, age, smoking habits, and other factors in normal healthy Korean population. The 99 volunteers included in the study and out of these, 36 volunteers were smoker and 63 volunteers were non-smoker aged between 20-59 years. All individual answered a questionnaire that assessed their general information including smoking habits and the extent of the environmental tobacco smoke (ETS) exposure, and blood samples were obtained. There was a statistically significant difference in the extent of DNA damage between smoker and non-smoker (p<0.001). A significant difference was also observed between male and female (p<0.001) and amongst the different group of age (p<0.005), however, correlation analysis showed that only smoking habit was a significant factor for DNA damage. No significant effect of smoking duration, number of cigarettes smoking a day, SPY (smoke pack years) in smokers and environmental tobacco smoke exposure in non-smokers on the status of DNA damage was observed.

• The Transactions of the Korean Institute of Electrical Engineers B
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• v.52 no.7
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• pp.343-351
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• 2003

In this paper, interleaved boost converter is applied as a first-stage converter in switch mode power supply. The first-stage converter plays a role to improve power factor. Interleaved Boost Power Factor Corrector(IBPFC) can reduce input current ripple as a single voltage control loop only without inner current loop, because input current is divided each 50% by two switching devices. Each converter cell is also operated in discontinuous current mode and inductor current of each converter is discontinuous. Total input current which is composed by each converter cell is continuous current. Thus, IBPFC is able to improve input current ripple. IBPFC operating in discontinuous current mode can be classified as six modes from switching state and be carried out state space averaging small signal modeling. A control transfer function is obtained according to the modeling. Not only steady-state characteristics but also dynamic characteristics is considered. Single voltage control loop is also constructed by the control transfer function. From experimental result, improvement of power factor and input current ripple are verified.

• Molecules and Cells
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• v.42 no.4
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• pp.356-362
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• 2019

The binding of MS2 bacteriophage coat protein (MCP) to MS2 binding site (MBS) RNA stem-loop sequences has been widely used to label mRNA for live-cell imaging at single-molecule resolution. However, concerns have been raised recently from studies with budding yeast showing aberrant mRNA metabolism following the MS2-GFP labeling. To investigate the degradation pattern of MS2-GFP-labeled mRNA in mammalian cells and tissues, we used Northern blot analysis of ${\beta}$-actin mRNA extracted from the Actb-MBS knock-in and $MBS{\times}MCP$ hybrid mouse models. In the immortalized mouse embryonic cell lines and various organ tissues derived from the mouse models, we found no noticeable accumulation of decay products of ${\beta}$-actin mRNA compared with the wild-type mice. Our results suggest that accumulation of MBS RNA decay fragments does not always happen depending on the mRNA species and the model organisms used.

• Bulletin of the Korean Chemical Society
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• v.14 no.2
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• pp.258-262
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• 1993

Two single crystals of fully dehydrated $Rb^+$ -exchanged zeolite A have been prepared by the reduction of all $Ca^{2+}$ ions in dehydrated $Ca_6$-A by rubidium vapor. Their structures were determined by single crystal X-ray diffraction methods in the cubic space group Pm3m (a=12.160(2) $^{\AA}$ and 12.166(2) $^{\AA}$) at 22(1)$^{\circ}$C. In these structures, 12.4(2) to 13.3(2) Rb species are found per unit cell, more than 12 Rb$^+$ ions needed to balance the anionic charge of the zeolite framework, indicating that the sorption $Rb^0$ has occurred. In each structure, three $Rb^+$ ions per unit cell are located at the centers of the 8-rings. Six to eight $Rb^+$ ions are found opposite the 6-rings on threefold axes, and three $Rb^+$ ions are found in a sodalite unit. About 0.5 $Rb^+$ ion lies opposite a 4-ring. The structural analysis indicates the presence of a triangular rubidium cluster in the sodalite cavities. The triangular rubidium clusters may be stabilized by the coordination to two and/or three rubidium ions in the large cavity. Therefore, this cluster may be viewed as $(Rb_5)^{4+}$ and/or $(Rb_6)^{4+}$.

• Journal of Microbiology
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• v.35 no.1
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• pp.61-65
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• 1997

A novel strategy was developed for isolating suppressors from sporulation-deficient mutants. The mutation in the BCY1 gene, which codes for the regulatory subunit of cAMP-dependent protein kinase, when homozygous, results in diploids being meiosis and sporulation deficient. Two plasmids, YCp-MAT.alpha. and YEp-SPOT7-lacZ, were introduced into MAT.alpha. BCY1$\^$+/ or MAT.alpha. bcy1 haploid cells. The transformant of the BCY1$\^$+/ haploid cell produced .betha.-galactosidase under nutrient starvation, but the bcy1 transformant did not. Using this system, the mutagenesis experiment performed on the bcy1 transformant strain resulted in a number of sporulation mutants that produced .betha.-galactosidase under nutrient starvation. One complementation group, sob1, was identified from the isoalted suppressor mutants and characterized as a single recessive mutation by tetrad analysis. Genetic analysis revealed that the sob1 mutation suppressed the sporulation deficiency, the failure to arrest at the G1 phase of the cell cecle, and the sensitivity to heat or nitrogen starvation caused by the bcy1 mutation. However, the sob1 mutation did not suppress the sporulation deficiency of ime1 and of ime2 diploids. These results suggest that the sob1 mutation affects a gene which functions as a downstream regulator in both meiosis and cell cycle regulation.

• Journal of the Korean Society for Geothermal and Hydrothermal Energy
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• v.17 no.4
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• pp.46-58
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• 2021

As accidents with thermal runaway (TR) of lithium-ion batteries occur sporadically, the safety concern is the main obstacle that hinders the large-scale applications of lithium ion batteries. In most accidents, the TR of a single cell occurred first, and then dissipated the heat to the surroundings and triggered the TR of adjacent cells, resulting in TR propagation. Therefore, it is important to understand the mechanism of TR propagation and determine the key parameters during TR propagation in a battery pack. In this study, we performed a numerical analysis on the thermal runaway phenomenon by cathode active materials and appearance sizes in cylindrical lithium-ion batteries using a two-dimensional analysis model. The model results showed that the TR propagation of 21700 type cells (21 mm diameter, 70 mm height) occurs more rapidly than 46800 type cells (46 mm diameter, 80 mm height) and the LFP cell has higher thermal safety than the NCM cell. Especially, we found that the effect of the separator on the occurrence of TR is negligible.