• The Korean Journal of Food And Nutrition
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• v.6 no.1
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• pp.1-7
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• 1993

19 Samples of koji-starters using in brewing were collected from Korea and Japan, and then 31 strains of koji-molds were isolated from them. After Identification of the Isolate, rice koji was made with each strains, and its saccharogenic activity, dextrogenic activity, proteolytic activity, acid Producing ability, browning reaction and flavor were tested. Among 31 strains of isolates, 10 strains were Identified as Asp nwamori var. kawachii, 18 strains as Asp. oryzae, 3 strains as Asp. usamii mkt. shirousamii. The koji-starters made in Korea were composed of single species of koji-mold with same strain, but those made in Japan were composed of the mixture of different two species or the mixture of different 2 ∼4 strains in same species. Judging from amylolytic and proteolytic ability by species, Asp. awamori var. kawachii H1, I1 and 11, Asp. owsae J2, L2, M2, P3 and P4, and Asp. usamii writ. shirousamii S1 were better than the others. Mold strains isolated from Korean koji-starters were much lower in amylolytic or proteolytic activity than those from Japanese koji-starters. The typical characteristics for the 3 species of koji-molds were that Asp. awamori var. kawachii was strong in acid producing ability, but week in amylolytic and proteolytic ability, that Asp. owzae had strong amylolytic activity and good aroma, but produced little amount of acid, and that Asp. usamii mut. shirousamii had strong Proteolytic activity but some off-flavor.

• Journal of Soil and Groundwater Environment
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• v.16 no.6
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• pp.46-57
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• 2011

In this study, analytical solution of multip-species transport equations coupled with a first-order reaction network under constant concentration boundary condition or total flux boundary condition is obtained using similarity transformation approach of Clement et al. (2000). The study shows the schematic process about how multi-species transport equations with first-order sequential reaction network is transformed through the similarity transformation approach into independent and uncoupled single species transport equations with first-order reaction. The analytical solution was verified through the comparison with popular commercial programs such as 2DFATMIC and RT3D. The analytical solution can be utilized in nuclear waste sites where radioactive contaminants and their daughter products occur and in industrial complex cities where chlorinated solvent such as PCE, TCE, and its biodegradation products produces. In addition, it can help the verification of the developed numerical code.

• Korean Journal of Veterinary Research
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• v.52 no.2
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• pp.75-82
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• 2012

We have developed and optimized a multiplex polymerase chain reaction (mPCR) for simultaneous detection of Brucella, Salmonella and Leptospira with high sensitivity and specificity. Three pairs of oligonucleotide primers were designed to specifically amplify the targeted genes of Salmonella, Leptospira and Brucella species with sizes of 521, 408 and 223 bp, respectively. The mPCR did not produce any nonspecific amplification products when tested against 15 related species of bacteria. The sensitivity of the mPCR was 100 fg for Brucella and 1 pg for both Salmonella and Leptospira species. In the field application, kidney, liver and spleen were collected from wild rats and stray cats and examined by mPCR. The high specificity and sensitivity of this mPCR assay provide a valuable tool for diagnosis and for the simultaneous and rapid detection of three zoonotic bacteria that cause disease in both humans and animals. Therefore, this assay could be a useful alternative to the conventional method of culture and single PCR for the detection of each pathogen.

• Mycobiology
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• v.34 no.1
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• pp.7-13
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• 2006

Arbuscular mycorrhizas (AM) have mutualistic symbiosis with plants and thus efforts have been placed on application of these symbiotic relationships to agricultural and environmental fields. In this study, AM fungi were collected from 25 sites growing with 16 host plant species in Korea and cultured with Sorghum bicolor in greenhouse condition. AM fungal spores were extracted and identified using both morphological and molecular methods. Using morphological characters, total 15 morpho-speices were identified. DNA was extracted from single spore of AM fungi and a partial region on 18S rDNA was amplified using nested PCR with AM fungal specific primers AML1/AML2. A total of 36 18S rDNA sequences were analyzed for phylogenetic analysis and 15 groups of AM fungi were identified using both morphological and molecular data of spores. Among the species, 4 species, Archaeospora leptoticha, Scutellospora castanea, S. cerradensis, S. weresubiae were described for the first time in Korea and two species in Glomus and a species in Gigaspora were not identified. Morphological and molecular identification of AM fungal spores in this study would help identify AM fungal community colonizing roots.

• Korean Journal of Plant Taxonomy
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• v.48 no.3
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• pp.195-200
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• 2018

Genetic assessments of rare and endangered species are among the first steps necessary to establish the proper management of natural populations. Transcriptome-derived single-sequence repeat markers were developed for the Korean endangered species Astilboides tabularis (Saxifragaceae) to assess its genetic diversity. A total of 96 candidate microsatellite loci were isolated based on transcriptome data using Illumina pair end sequencing. Of these, 26 were polymorphic, with one to five alleles per locus in 60 individuals from three populations of A. tabularis. The observed and expected heterozygosity per locus ranged from 0.000 to 0.950 and from 0.000 to 0.741, respectively. These polymorphic transcriptome-derived simple sequence repeat markers would be invaluable for future studies of population genetics and for ecological conservation of the endangered species A. tabularis.

• Genomics & Informatics
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• v.11 no.4
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• pp.272-276
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• 2013

Sequence analysis of the 16S rRNA gene has been widely used for the classification of microorganisms. However, we have been unable to clearly identify five Flavobacterium species isolated from a freshwater by using the gene as a single marker, because the evolutionary history is incomplete and the pace of DNA substitutions is relatively rapid in the bacteria. In this study, we tried to classify Flavobacterium species through multilocus sequence analysis (MLSA), which is a practical and reliable technique for the identification or classification of bacteria. The five Flavobacterium species isolated from freshwater and 37 other strains were classified based on six housekeeping genes: gyrB, dnaK, tuf, murG, atpA, and glyA. The genes were amplified by PCR and subjected to DNA sequencing. Based on the combined DNA sequence (4,412 bp) of the six housekeeping genes, we analyzed the phylogenetic relationship among the Flavobacterium species. The results indicated that MLSA, based on the six housekeeping genes, is a trustworthy method for the identification of closely related Flavobacterium species.

• Korean Journal of Breeding Science
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• v.43 no.4
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• pp.265-272
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• 2011

This study is to generate SCARs markers for identification of Perilla species. A SCAR is a genomic DNA fragment at a single genetically defined locus that is identified by PCR amplification using a pair of specific oligonucleotide primers. We derived SCARs by sequencing and cloning the both ends of the amplified products of RAPD markers. Sixteen sequence-specific primers were synthesized from eight RAPD markers, which were completely sequenced. We developed the species-specific SCAR markers which could be used successfully in detecting genetic variation in four Perilla species. These markers could be used to verify species-origins of various forms of Perilla germplasms.

• ALGAE
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• v.37 no.1
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• pp.33-47
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• 2022

Two new species of Pseudostaurosiropsis are described based on material collected from rivers in southern United States of America, P. californicus sp. nov. and P. elaboratus sp. nov. Both species have specific characters that set them apart from taxa currently ascribed to the genus. Pseudostaurosiropsis californicus sp. nov. has lanceolate valves with subrostrate, broadly rounded apices, an axial area at the same level as the virgae in internal and external views and both are at the same level as striae in external view, the spine tips are dentate and their growth is produced by filamentous extensions, and it has equal-sized apical pore fields on both valve extremes. On the other hand, P. elaboratus sp. nov. has heavily silicified valves, the virgae are slender than the striae in outer view and wider in inner view, it only has a single, externally occluded apical pore field on one valve extreme, and the spines have a solid core. All species within Pseudostaurosiropsis are contrasted with one another and unique features are described for each based on literature and newly collected image data from type material. The genus is reconsidered and two distinguishing features are recognized: rotae externally occluding the areolae and areolae that are funnel-shaped. These features are contrasted with those in other genera and additional published species that should be included in Pseudostaurosiropsis are discussed.

• Animal Systematics, Evolution and Diversity
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• v.39 no.4
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• pp.176-211
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• 2023

Nine copepod species of the family Artotrogidae Brady, 1880 are described or redescribed from Korea. Included new species are two in the genus Artotrogus Boeck, 1859, one each in the genera Bradypontius Giesbrecht, 1895, Cryptopontius Giesbrecht, 1899, Neopontius Scott T., 1898 and Chejupontius n. gen., and two in the genus Sewellopontius Ummerkutty, 1966. Chejupontius n. gen. resembles Ascidipontius Kim I. H., 1996, Pulicitrogus Kim I. H., 1998, and Sewellopontius in having reduced armature of leg 1, but is distinguished from these and other genera of the family by the large, mediodistally expanded protopod of leg 5 and by a combination of characters exhibited in leg 1 that the first exopodal segment lacks an inner seta, the second exopodal segment lacks an outer spine, the third exopodal segment is armed with three spines plus one to three setae, and the second endopodal segment is armed with a single inner seta. Pteropontius cristatus Giesbrecht, 1895 known previously only from the Mediterranean Sea is redescribed, based on a newly collected specimen from Korea, and P. trimerus Kim I. H., 1996 is removed to the genus Sewellopontius. A short note is given for the epibiotic suctorian Thecacineta calix (Schroder, 1907) which was found attached to the antennule of a new copepod species.

• Journal of Species Research
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• v.13 no.1
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• pp.89-99
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• 2024

Larger benthic foraminifera (LBF) are a group of marine organisms that inhabit oligotrophic subtropicaltropical photic zones, commonly possess relatively large shells (i.e., tests) with a complex internal structure and host photosynthetic endosymbionts. In this study, we provide a new report of a LBF species belonging to the genus Sorites Ehrenberg, 1839, with a brief description and images of the test. The sediment samples were collected by SCUBA diving from the east coast of Jeju Island (South Korea). The discoidal test shape and annular series of chamberlets with a single row of apertures surrounded by rim indicate that these specimens belong to the genus Sorites, and this is the first record of the genus in Korea. The arrangement of early chamberlets, septular disposition, and the aperture details suggest that the specimens can be assigned to Sorites orbiculus(Forsskål in Niebuhr, 1775). Only three LBF species, including the present species, have been reported from Korean waters. The present study provides additional information for the evaluation of foraminiferal diversity including LBF in Korea and suggests the necessity of continuous investigations to understand the diversity of Sorites species in Korea.