• 대한의생명과학회지
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• 제8권4호
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• pp.211-215
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• 2002

The baculovirus/Sf9 cell expression can be employed as a powerful system for producing large amounts of the human erythrocyte glucose transporter, GLUT1 heterologously In order to exploit the system further, it is necessary to develop a convenient method for demonstrating that the transporter expressed in insect cells is biologically active. To achieve this, we have expressed the human CLUT1 in insect cells and photolabelled the expressed protein with [$^3$H] cytochalasin B, a potent inhibitor of the human erythrocyte glucose transporter. Subsequently, the labelled proteins were analysed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Membranes labelled with [$^3$H] cytochalasln B in the presence of L-Glucose yielded a single sharp peak of labelling of apparent $M_r$ 45,000 on SDS/polyacrylamide gels. The mobility of this peak corresponded exactly to that of the band detected by anti-glucose transporter antibodies on Western blots of membranes prepared from insect cells infected with recombinant virus. In addition, the sharpness of the radioactive peak provides further evidence for the conclusion that the expressed protein is much less heavily and heterogeneously glycosylated than its erythrocyte counterpart. No peak of labelling was seen with the membranes prepared from non-infected Sf9 cells. Furthermore, the incorporation of label into this peak was completely inhibited by the presence of 500 mM-D-Glucose during tile photolabelling procedure, showing the stereoselectivity of the labelling. These evidences clearly show that human glucose transporter expressed in insect cells exhibits native-like biological activity, and that photolabelling with [$^3$H] cytochalasin B can be a convenient means for analysing the biological activity of the transport protein expressed in insect cells.

• 대한수의학회지
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• 제31권3호
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• pp.329-335
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• 1991

The filter elution technique was used to assay $^{60}Co$ $\gamma$ ray-induced DNA strand breaks(SB) in EL4 mouse leukemia cell and mouse spleen lymphocyte. The lymphocytes were stimulated with lipopolysaccharide (LPS, $20{\mu}g/ml$) to label $[^3H]$ thymidine. EL4 cells and lymphocytes in suspension were exposed at $0^{\circ}C$ to 0Gy, 1Gy, 5Gy, 10Gy or l5Gy for DNA single strand breaks(SSB) assay and 0Gy, 25Gy, 50Gy, 75Gy or 100Gy for DNA double strand breaks(DSB) assay of $^{60}Co$ radiation and elution procedure was performed at pH12.1 and 9.6. The number of DNA strand breaks increased with increasing doses of r rays. The strand scission factor(SSF) was estimated in each experiment (eluted volume 21ml). The slope of SSB EL4 cells was $0.01301{\pm}0.00096Gy^{-1}$ (n=5), the slope of SSB for lymphocytes was $0.01097{\pm}0.00091Gy^{-1}$ (n=5) and the slope of DSB for lymphocytes was $0.001707{\pm}0.0000573Gy^{-1}$ (n=5). Thus EL4 cells were more sensitive to induction of DNA SSB by ionizing radiation than lymphocytes (p<0.005). The ratio of slope of dose-response relationship (SSF versus dose) of lymphocytes DNA SSB as compared with the slope of DNA DSB was 6.4.

• 한국철도학회논문집
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• 제18권2호
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• pp.149-156
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• 2015

도시철도 승객의 수입금 정산 배분을 위하여 승객의 경로를 파악할 필요가 있다. 승객의 예상 이동경로를 파악을 위해 다양한 경로탐색기법들이 있지만, 링크기반 k-경로탐색기법이 많이 활용되고 있다. 그러나 기존의 k-경로탐색기법은 통행 및 도보시간, 환승 패널티 등의 변수에 따라 다양한 최적경로가 존재하는 문제점이 있고 특히, 급행열차를 선호하는 승객의 특성, 열차의 특성 등을 반영하지 못하는 한계를 지니고 있다. 본 연구에서는 이를 보완하기위하여 교통카드자료를 기반으로 실제로 이용한 하나의 경로를 탐색하는 방안을 제시하였다. 또한, 각 철도운영기관의 열차스케줄을 연계하여 최적경로를 탐색하는 방안도 제안하였다.

• 한국임상약학회지
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• 제23권3호
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• pp.239-247
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• 2013

Background: Fixed drug combinations are formulations containing two or more active ingredients in a single dosage form. Such combination therapies are commonly applied to improve efficacy, reduce adverse events and replace co-administration, etc. National and international guidelines for hypertension treatment recommend addition of other classes of antihypertensive drugs rather than incremental dose of mono-therapy, when blood pressure is not adequately controlled. Thus, many dual combinations of antihypertensive drugs have been approved and pharmaceutical companies are recently interested in developing antihypertensive triple combinations. Clinical trial designs for the fixed combinations are various depending on the target patients, dosage and clinical endpoints. Thereby, further discussions for the clinical trials of antihypertensive triple therapies are required regarding the indication claimed. Conclusion: This article provides a review for the assessment of the label and medical reports of the clinical trials on antihypertensive triple therapies in advanced foreign countries.

• Journal of Multimedia Information System
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• 제9권2호
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• pp.113-120
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• 2022

Mongolian folk songs are inspired by Mongolian labor songs and are classified into long and short songs. Mongolian long songs have ancient origins, are rich in legends, and are a great source of folklore. So it was inscribed by UNESCO in 2008. Mongolian written literature is formed under the direct influence of oral literature. Mongolian long song has 3 classes: ayzam, suman, and besreg by their lyrics and structure. In ayzam long song, the world perfectly embodies the philosophical nature of world phenomena and the nature of human life. Suman long song has a wide range of topics such as the common way of life, respect for ancestors, respect for fathers, respect for mountains and water, livestock and animal husbandry, as well as the history of Mongolia. Besreg long songs are dominated by commanded and trained characters. In this paper, we proposed a method to classify their 3 types of long songs using machine learning, based on their lyrics structures without semantic information. We collected lyrics of over 80 long songs and extracted 11 features from every single song. The features are the name of a song, number of the verse, number of lines, number of words, general value, double value, elapsed time of verse, elapsed time of 5 words, and the longest elapsed time of 1 word, full text, and type label. In experimental results, our proposed features show on average 78% recognition rates in function type machine learning methods, to classify the ayzam, suman, and besreg classes.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제25권2호
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• pp.147-162
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• 2022

Purpose: To design a prospective study on endovascular closure of congenital portosystemic shunts. The primary endpoint was to assess the safety of endovascular closure. The secondary endpoint was to evaluate the clinical, analytical and imaging outcomes of treatment. Methods: Fifteen patients (age range: 2 days to 21 years; 10 male) were referred to our center due to congenital portosystemic shunts. The following data were collected prior to treatment: age, sex, medical history, clinical and analytical data, urine trimethylaminuria, abdominal-US, and body-CT. The following data were collected at the time of intervention: anatomical and hemodynamic characteristics of the shunts, device used, and closure success. The following data were collected at various post-intervention time points: during hospital stay (to confirm shunt closure and detect complications) and at one year after (for clinical, analytical, and imaging purposes). Results: The treatment was successful in 12 participants, migration of the device was observed in two, while acute splanchnic thrombosis was observed in one. Off-label devices were used in attempting to close the side-to-side shunts, and success was achieved using Amplatzer™ Ductus-Occluder and Amplatzer™ Muscular-Vascular-Septal-Defect-Occluder. The main changes were: increased prothrombin activity (p=0.043); decreased AST, ALT, GGT, and bilirubin (p=0.007, p=0.056, p=0.036, p=0.013); thrombocytopenia resolution (p=0.131); expansion of portal veins (p=0.005); normalization of Doppler portal flow (100%); regression of liver nodules (p=0.001); ammonia normalization (p=0.003); and disappearance of trimethylaminuria (p=0.285). Conclusion: Endovascular closure is effective. Our results support the indication of endovascular closure for side-to-side shunts and for cases of congenital absence of portal vein.

• 농업과학연구
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• 제50권4호
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• pp.697-711
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• 2023

The global waste crisis has been escalating and its consequent impact on soil, water, air pollution, and eventually climate change acceleration has shed light on the importance of reducing waste. Amidst COVID-19 and the following surge in single-use plastics for food delivery, waste generation is on the incline. Companies and governments have embarked on developing various eco-friendly packaging technologies, but their effectiveness on the consumers is vague as definitions of eco-friendly packaging are vague, and research on its link to purchase intention remains scarce. Thus, the adoption of eco-friendly packaging has been slow. To address this issue, this study analyzes the awareness and purchase intention of four visual attributes of eco-friendly packaging-material, verbal statement, eco-label, and color-along with the environmental consciousness among undergraduate university students in Korea through online surveys and the ordered logit regression model. The study distinguished the attributes into evidence-based and conjectural categories. The findings revealed that eco-friendly visual attributes had a positive effect on purchase intention amongst undergraduate students in Korea; however the level of environmental consciousness had marginal effect on the purchase intention of eco-friendly visual attributes. The level of effectiveness also varied with each visual element. Analyses revealed that visual attributes to eco-friendly material had marginal effect on purchase intention; color was deemed not an "Eco-friendly attribute" by most students, and although eco-friendly labels were deemed as an eco-friendly attribute, trust in the labels varied according to environmental consciousness. These findings have implications for businesses and policymakers aiming to promote eco-friendly consumption within packaged food products.

• 한국간담췌외과학회지
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• 제28권2호
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• pp.238-247
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• 2024

Backgrounds/Aims: Prolonged use of steroids after liver transplantation (LT) significantly increases the risk of diabetes or cardiovascular disease, which can adversely affect patient outcomes. Our study evaluated the effectiveness and safety of early steroid withdrawal within the first year following LT. Methods: This study was conducted as an open-label, multicenter, randomized controlled trial. Liver transplant recipients were randomly assigned to one of the following two groups: Group 1, in which steroids were withdrawn two weeks posttransplantation, and Group 2, in which steroids were withdrawn three months posttransplantation. This study included participants aged 20 to 70 years who were scheduled to undergo a single-organ liver transplant from a living or deceased donor at one of the four participating centers. Results: Between November 2012 and August 2020, 115 patients were selected and randomized into two groups, with 60 in Group 1 and 55 in Group 2. The incidence of new-onset diabetes after transplantation (NODAT) was notably higher in Group 1 (32.4%) than in Group 2 (10.0%) in the per-protocol set. Although biopsy-proven acute rejection, graft failure, and mortality did not occur, the median tacrolimus trough level/dose/weight in Group 1 exceeded that in Group 2. No significant differences in safety parameters, such as infection and recurrence of hepatocellular carcinoma, were observed between the two groups. Conclusions: The present study did not find a significant reduction in the incidence of NODAT in the early steroid withdrawal group. Our study suggests that steroid withdrawal three months posttransplantation is a standard and safe immunosuppressive strategy for LT patients.

• Translational and Clinical Pharmacology
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• 제25권3호
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• pp.153-156
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• 2017

UI14SDF100CW is a chewable tablet of sildenafil citrate, which was developed to improve compliance through convenience of administration. The purpose of this study was to compare the pharmacokinetic (PK) properties of sildenafil citrate chewable tablets (UI14SDF100CW) and conventional sildenafil citrate film-coated tablets ($Viagra^{(R)}$, Pfizer). A randomized, open-label, single dose, two-treatment, two-period, two-way crossover study was conducted in 60 healthy male volunteers. In each period, the subjects received a single oral dose of UI14SDF100CW or $Viagra^{(R)}$ (both tablets contain 140.45 mg of sildenafil citrate, which is equivalent to 100 mg of sildenafil). Serial blood samples were collected up to 24 h post-dose for PK analysis. The plasma concentration of sildenafil was determined using a validated HPLC-MS/MS assay. PK parameters of sildenafil were calculated using non-compartmental methods. The plasma concentration-time profiles of sildenafil in both formulations were similar. For UI14SDF100CW, the $C_{max}$ and $AUC_{last}$ of sildenafil were $1068.69{\pm}458.25$ (mean${\pm}$standard deviation) mg/L and $3580.59{\pm}1680.29h{\cdot}mg/L$, and the corresponding values for $Viagra^{(R)}$ were $1146.84{\pm}501.70mg/L$ and $3406.35{\pm}1452.31h{\cdot}mg/L$, respectively. The geometric mean ratios (90% confidence intervals) of UI14SDF100CW to $Viagra^{(R)}$ for $C_{max}$ and $AUC_{last}$ were 0.933 (0.853-1.021) and 1.034 (0.969-1.108), respectively, which met the bioequivalence criteria of Korean regulatory agency. In conclusion, UI14SDF100CW and $Viagra^{(R)}$ showed similar PK properties. Therefore, UI14SDF100CW can be an alternative to sildenafil for the treatment of erectile dysfunction, providing better compliance.

• 대한원격탐사학회지
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• 제38권5_3호
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• pp.967-977
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• 2022

산불의 발생과 강도는 기후 변화로 인하여 증가하고 있다. 산불 연기에 의한 배출가스 대기질과 온실 효과에 영향을 미치는 주요 원인 중 하나로 인식되고 있다. 산불 연기의 효과적인 탐지를 위해서는 위성 산출물과 기계학습의 활용이 필수적이다. 현재까지 산불 연기 탐지에 대한 연구는 구름 식별의 어려움 및 모호한 경계 기준 등으로 인한 어려움이 존재하였다. 본 연구는 우리나라 환경위성 센서인 Geostationary Environment Monitoring Spectrometer (GEMS)의 Level 1, Level 2 자료와 기계학습을 이용한 산불 연기 탐지를 목적으로 한다. 2022년 3월 강원도 산불을 사례로 선정하여 산불 연기 레이블 영상을 생성하고, 랜덤 포레스트 모델에 GEMS Level 1 및 Level 2 자료를 투입하여 연기 픽셀 분류 모델링을 수행하였다. 훈련된 모델에서 입력변수의 중요도는 Aerosol Optical Depth (AOD), 380 nm 및 340 nm의 복사휘도 차, Ultra-Violet Aerosol Index (UVAI), Visible Aerosol Index (VisAI), Single Scattering Albedo (SSA), 포름알데히드, 이산화질소, 380 nm 복사휘도, 340 nm 복사휘도의 순서로 나타났다. 또한 2,704개 픽셀에 대한 산불 연기 확률(0≤p≤1) 추정에서 Mean Bias Error (MBE)는 -0.002, Mean Absolute Error (MAE)는 0.026, Root Mean Square Error (RMSE)는 0.087, Correlation Coefficient (CC)는 0.981의 정확도를 보였다.