• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1995년도 Proceedings of the Korea Automation Control Conference, 10th (KACC); Seoul, Korea; 23-25 Oct. 1995
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• pp.39-42
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• 1995

A new methodology for representing the interaction between machines and the interlock signals required in FMCs has been developed. Object-Oriented Philosophies (OOPs) lend themselves to the development of such a scheme. A methodology developed here regards the tasks to be performed by the cell or any of its constituent machines for being primal. Sensory signals indicating the changes of state pf machines are used to trigger or initiate tasks. A task may be simple and require a relatively short time to execute, or may be complex and lengthy. This methodology may be depicated by a set of diagrams called Task Initiation Diagram (TID) and their accompanying rules.

• 한국정밀공학회지
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• 제25권3호
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• pp.147-156
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• 2008

A new approach based on cone prism intersection method combined with sorting algorithm is proposed for the fast and robust signed distance field computation. In the method, the space bounding the geometric model composed of triangular net is divided into multiple smaller cells. For the efficient calculation of distance fields, valid points among the triangular net which will generate minimum distances with current cell are selected by checking the intersection between current cell and cone prism generated at each point. The method is simple to implement and able to achieve an order of magnitude improvement in the computation time as compared to earlier approaches. Further the method is robust in handling the traditional sign problems. The validity of the suggested method was demonstrated by providing numerous examples including Boolean operation, shape deformation and morphing of complex geometric models.

• KSBB Journal
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• 제13권4호
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• pp.456-460
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• 1998

Fed-batch fermentations were carried out in order to improve the efficiency of hirudin production by recombinant Saccharomyces cerevisiae. A fed-batch fermentation done with the optimized semi-synthetic medium resulted in a maximum hirudin concentration of 342mg/$\ell$ by keeping a galactose concentrations between 10 and 30g/$\ell$ which corresponded to a 11.4-fold increase in hirudin concentration compared with the simple bach fermentation done with the same medium. Comparison of the chromatographic pattern of proteins in the growth medium clearly showed that the use of the semi-synthetic medium is more advantageous for separation of hirudin than the case o fusing the complex medium. Continuous cell recycle fermentation done at dilution rate of 0.1h-1 and an inlet galactose concentration of 100g/$\ell$ yielded a maximum hirudin productivity of 19.1mg hirudin/$\ell$$.$h.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2008년도 학술발표회 논문집
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• pp.1752-1756
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• 2008

A simple, accurate and efficient mesh generation technique, the cut-cell method, is able to represent an arbitrarily complex geometry. Both structured and unstructured grid meshes are used in this method. First, the numerical domain is constructed with regular Cartesian grids as a background grid and then the solid boundaries or bodies are cut out of the background Cartesian grids. As a result, some boundary cells can be contained two numerical conditions such as the flow and solid conditions, where the special treatment is needed to simulate such physical characteristics. The HLLC approximate Riemann solver, a Godunov-type finite volume method, is employed to discretize the advection terms in the governing equations. Also, the TVD-WAF method is applied on the Cartesian cut-cell grids to stabilize numerical results. Present method is validated for the rectangular dam break problems. Initially, a conventional grid is constructed with the Cartesian regular mesh only and then applied to the dam-break flow simulation. As a comparative simulation, a cut-cell grids are applied to represent the flow domain rotated with arbitrary angles. Numerical results from this study are compared with the results from the case of the Cartesian regular mesh only. A good agreement is achieved with other numerical results presented in the literature.

• 한국패류학회지
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• 제12권2호
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• pp.109-121
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• 1996

Histochemical experiment was carry out respectively to confirm the properties of the salis (Achatina fulica and Incilaria fruhstorferi). SDS-PAGE was carried out to compare and invertigate the distribution aspects of protein patterns between the two species. Five types(A, B, F, H and I)of gland cells with four neutral mucopolysaccharide cells and one acid mucopolysaccharide cells and one acid mucopolysaccharide cell were observed in acinous of Achatina fulica, while six types were observed in acinous of Incilaria fruhstorferi: ond acid mucopolysaccharide cell(type-A) and four neutral mucopolysaccharide cells(type-B, C, D and F) and one cell that acid mucopolysaccharide is only mimbrane that surrounded granule(type-E). The results are follows:The thpe-A fland cell is commonly observed between the two species. The type-A gland cell in Achatina fulica possesses a nucleus with a developed heterdchromatin, and the cytoplasm was filled with round granules. The granules were surrounded with an uncertain boundary mimbrane and confirmed with neutral mucopolysaccharides, but is confirmed acid mucopolysaccharide in Incilaria fruhstorferi.The type-B gland cell is obwerved in the two species, too. The type-B gland cell in Achatina fulica was round shaped, and included an evenly alrge nucleus. The uncleoplasm included granules that were confirmed in the neutral mucopolysaccharides of the two species. The type-C and D gland cells exist only in Incilaria fruhstorferi, nucleoplasm was well developed heterochromatins. The type-E gland cell appears in the acinous surrounded the salivary gland of Incilaria fruhstorferi. Thdse granules appear irregular irregular shape and size and the cytoplasm is formed in alveolar. The type-F gland cells are commonly observed in the salivary glands of the two species. They are similar with the type-B gland cell, but the granular shape is comparatively small and irregular, and possess the neutral mucos granules. The type-H gland cells are mainly seen in only Achatina, and in nucleus is a well developed heterochromatin. The cytoplasm is filled with round small granules with acid mucopolysaccharide for alcianophilia observed. The type-I cell was small cell with an irregular shape and only observed in the gland cells of Achatina fulica. The heterochromatins were developed in the nucleus and the granules are not observed in cytoplasm.Secretory ducts of saliva are composed of the interlobular duct and interlobar secretory duct. In Achatina fulica the interlobular duct consists of a simple cuboidal epithelium, while the endothelium of intralobar secretory duct of Incilaria fruhstorferi consists of a simple squamous epithelium and in the cytoplasm is filled with granules(type-G secretory cell). A SDS-PAGE was carried out to confirm that the protein band pattern consist of salivary gland. In conclusions, five more bands in Achatina fulica and three bands in Incilaria fruhstorferi were confirmed in MW<29 kDa. one main band coincides comparatively with both and is between 29-45 kDa. There are four main bands in Achatina fulica and two main bands in Incilaria fruhstorferi between 45-66.5 kDa respectively. The bands in Achatina fulica seem more complex than in incilaria fruhstorferi.

• 청정기술
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• 제17권1호
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• pp.25-30
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• 2011

본 연구 논문은 수용액 기반의 청정 표면 개질 기술을 이용하여 세포칩을 제작하는 방법에 관한 것이다. 세포칩의 활용범위는 유전학, 의생물학, 세포생물학 등과 같은 기초학문과 더불어 암 진단 및 치료에 대한 유용한 도구로 응용 가능성을 가지게 된다. 기존의 세포 칩 제작을 위해서는 다량의 유기용매의 사용, 반도체 공정의 복잡성, 고가의 장비 등을 사용함으로 인해 경제적 손실과 환경적 악영향을 주었다. 본 연구에서는 수용액 기반의 청정 표면 개질 기술과 마이크로 컨택트 프린팅 방법을 이용한 세포 패터닝 기술을 융합하여 매우 손쉬운 세포 칩 구현을 하는 기반기술을 제시하였다. 이 세포칩을 이용하여 암세포와 정상세포간의 세포표면에서 발현되는 다양한 탄수화물 및 그의 유도체의 발현양의 차이를 분석할 수 있었다. 이를 바탕으로 새로운 암진단 기술 및 기초 의공학 기술에 활용하고자 한다.

• Composites Research
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• 제32권5호
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• pp.211-215
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• 2019

정적인 상태인 체외 환경(in vitro)에서의 세포배양 과정은 실제 생체 내 환경에서의 세포발달과정과는 많은 차이가 존재한다. 따라서, 체내 환경의 정밀한 모사를 위해서는, 기계적인 자극을 세포에 전달하여 줄 수 있는 동적 세포배양장치가 필수적이다. 하지만 기존의 동적 세포배양장치에는 튜브, 펌프, 모터 등의 비교적 복잡한 장치들을 필요로 하였으며, 전달되는 기계적 자극도 단순한 형태였다. 본 연구에서는 단순한 장치로 구동되는 동적 세포배양장치를 위하여 전기활성고분자(EAP) 구동기를 동력원으로 하는 소형 동적 세포배양장치를 설계하였다. 이 장치는 다양한 기계적 자극을 세포에 전달하는 것이 가능하다.

• 한국복합재료학회:학술대회논문집
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• 한국복합재료학회 2003년도 추계학술발표대회 논문집
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• pp.71-74
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• 2003

The cell modeling homogenization method to derive the constitutive equation considering the microstructures of the fiber reinforced composites has been previously developed for composites with simple microstructures such as 2D plane composites and 3D rectangular shaped composites. Here, the method has been further extended for 3D circular braided composites, utilizing B-spline curves to properly describe the more complex geometry of 3D braided composites. For verification purposes, the method has been applied for orthotropic elastic properties of the 3D circular braided glass fiber reinforced composite, in particular for the tensile property. Prepregs of the specimen have been fabricated using the 3D braiding machine through RTM (resin transfer molding) with epoxy as a matrix. Experimentally measured uniaxial tensile properties agreed well with predicted values obtained fer two volume fractions.

• Clinical and Experimental Reproductive Medicine
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• 제20권1호
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• pp.9-17
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• 1993

This study was carried out investigate the effect of water quality and the kind of media on the in vitro development of 1-cell and stage mouse embryos. $F_1$ hybrid mice were superovulated and timely mated. 1-cell stage and 2-cell stage mouse embryos were recruited and taken into Ham's F-10 or m-KRB media which was made of two of two kinds of water having different quality, highly purified water and tap water. 2-cell stage embryos grew up well in vitro to blastocyst or hatching blastocyst regardless of the composition of culture media, but 1-cell stage mouse embryo didn't develop well to blastocyst or hatching blastocyst in simple media like m-KRB. These results meant in vitro devleopment of 1-cell stage mouse embryo neded complex media like Ham's F-10 which contained abundant protein components. In case of quality control for water, in vitro fertilization program. observation of in vitro development of 2-cell mouse embryos up to blastocyst or hatching blastocyst media such as m-KRB would be efficatious in detecting the difference of water quality.

• 한국수정란이식학회지
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• 제6권1호
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• pp.33-40
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• 1991

In vitro developmental ability of early preimplantation monse embryos was shown to be depend on the embryonic stages, media and snpplements and their interaction(Experiment 1). The development of I-cell embryos were more promoted in the complex medinm(Ham's Fl0) than in the simple one(m-KRB), but that of 2-cell embryos showed the reverse effect. The bovine serum albumin(BSA) as a medium snpplement more promoted the development of I- and 2-cell embryos, compared with human fetal cord serum(HCS). On the other hand, the harmful effect of HCS was especially shown on the early cleavage in the embryonic development of the two stages. The effect of serum, in the respect of interaction between media and snpplements. was also more significantly appeared in m-KRB than Ham's Fl0. In the experiment 2, when the harmful effect of HCS was compared with that of fetal bovine serum(FBS), the former more promoted the development of l - and 2-cell embryos than the latter. The effect of HCS was more significantly shown in the development of I-cell than that of 2-cell embryos. Conclusively, as I- and 2-cell embryos were different in the requirements for the in vitro development. the optimal medium and supplement have to be selected for each embryonic stage. It is also respected to the better result if it take into consideration into the kinds of sera when serum is used for culture of early preimplantation embryos.