Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Mouse Embryo Culture used in Quality Control of Water for Human in Vitro Fertilization : The One-cell Stage Versus the Two-cell Stage Model

수질에 대한 1-세포기 및 2-세포기 생쥐배아를 이용한 생물학적 정도관리에 관한 연구

  • Lee, Ye-Kyung (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
  • Chung, Hye-Won (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
  • Kim, Hyung-Mee (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
  • Oh, Seung-Eun (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
  • Son, Young-Soo (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
  • Yu, Han-Ki (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
  • Woo, Bock-Hee (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University)
  • 이예경 (이화여자대학교 의과대학 산부인과학교실) ;
  • 정혜원 (이화여자대학교 의과대학 산부인과학교실) ;
  • 김향미 (이화여자대학교 의과대학 산부인과학교실) ;
  • 오승은 (이화여자대학교 의과대학 산부인과학교실) ;
  • 손영수 (이화여자대학교 의과대학 산부인과학교실) ;
  • 유한기 (이화여자대학교 의과대학 산부인과학교실) ;
  • 우복희 (이화여자대학교 의과대학 산부인과학교실)
  • Published : 1993.06.04
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Abstract

This study was carried out investigate the effect of water quality and the kind of media on the in vitro development of 1-cell and stage mouse embryos. $F_1$ hybrid mice were superovulated and timely mated. 1-cell stage and 2-cell stage mouse embryos were recruited and taken into Ham's F-10 or m-KRB media which was made of two of two kinds of water having different quality, highly purified water and tap water. 2-cell stage embryos grew up well in vitro to blastocyst or hatching blastocyst regardless of the composition of culture media, but 1-cell stage mouse embryo didn't develop well to blastocyst or hatching blastocyst in simple media like m-KRB. These results meant in vitro devleopment of 1-cell stage mouse embryo neded complex media like Ham's F-10 which contained abundant protein components. In case of quality control for water, in vitro fertilization program. observation of in vitro development of 2-cell mouse embryos up to blastocyst or hatching blastocyst media such as m-KRB would be efficatious in detecting the difference of water quality.

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