• Journal of Microbiology and Biotechnology
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• 제17권6호
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• pp.1031-1035
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• 2007

Prostate specific $antigen-{\alpha}_1-antichymotrypsin$ was detected by a double-enhancement strategy involving the exploitation of both colloidal gold nanoparticles (AuNPs) and precipitation of an insoluble product formed by HRP-biocatalyzed oxidation. The AuNPs were synthesized and conjugated with horse-radish peroxidase-PSA polyclonal antibody by physisorption. Using the protein-colloid for SPR-based detection of the PSPJACT complex showed their enhancement as being consistent with other previous studies with regard to AuNPs enhancement, while the enzyme precipitation using DAB substrate was applied for the first time and greatly amplified the signal. The limit of detection was found at as low as 0.027 ng/ml of the PSA/ACT complex (or 300 fM), which is much higher than that of previous reports. This study indicates another way to enhance SPR measurement, and it is generally applicable to other SPR-based immunoassays.

• 전자공학회논문지S
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• 제34S권1호
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• pp.36-42
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• 1997

This paper proposes a high-speed contention resolution scheme featuring high flexibility to the bursty traffic for an input buffering ATM switching architecture and its hardware strategy. The scheme is based on the threshold on the occupancy of the input buffer. As the proposed scheme utilizes the threshold, it has high flexibility to the fluctuations of the input traffic. The hardware strategy for the proposed policy is provided with the aim of the simple structure that achieves the reduction of the signal path and the power consumption. The performance on the average buffer size of the proposed policy is performed and compared with the conventional schame under the bursty traffic through both the analysis based on the markov hain and the simulation. The relations among the parameters on the proposed policy is analyzed to improve the performance.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1990년도 한국자동제어학술회의논문집(국제학술편); KOEX, Seoul; 26-27 Oct. 1990
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• pp.1337-1342
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• 1990

This paper deals with control system design strategy for electrolmaginetic suspension (E.M.S.) system. For a successful control of E.M.S. system, the nature of E.M.S. system is deeply studied in the view point of non-linear, open-loop unstable, time-varying, non-minimum phase system. To find a special control treatment for E.M.S. system, analyses and simulations for various models are carried out. As one of the successful candidates, adaptive control concept is introduced and sample hardware system using digital signal processor is implemented.

• 대한전기학회논문지:시스템및제어부문D
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• 제50권3호
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• pp.120-129
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• 2001

A two-degree-of-freedom quarter-car model is used as the basis for LQ and the proposed controller design for a semi-active suspension. The LQ controller results in the best rms performance trade-offs(as defined by performance index) between ride, handling and packaging requirements. In LQ controller, however, the conflict between road holding and ride comfort remains. The adaptive semi-active suspension control based on the road frequency are introduced in this paper. With this method, the trade-off between road holding and ride comfort can be relaxed. The road frequency is estimated by wavelet transform if rattle space signal. The simulation results show that the proposed controller is superior to the conventional LQ controller.

• Bulletin of the Korean Chemical Society
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• 제35권10호
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• pp.3047-3051
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• 2014

In this paper, we describe a new method for the selective extraction and quantification of glutathione (GSH) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and maleimide-presenting gold nanoparticles (Mal-AuNPs). Our strategy utilizes the Michael addition to selectively extract GSH, from chosen samples, onto the maleimide of Mal-AuNPs. After the extraction step, the GSH bound to the AuNPs was analyzed by MALDI-TOF MS in the presence of an internal standard which was prepared by reacting Mal-AuNPs with isotope-labeled GSH ($GSH^*$). The $GSH^*$ has the same structure as GSH but a higher molecular weight, and therefore, enables absolute quantification of GSH by comparing the mass signal intensities of the GSH- and $GSH^*$-conjugated alkanethiols. Our strategy was verified by analyzing GSH-spiked fetal bovine serum and NIH 3T3 cells.

• Molecules and Cells
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• 제24권2호
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• pp.268-275
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• 2007

Protein phosphorylation plays a key role in signal transduction in cells. Since phosphoproteins are present in low abundance, enrichment methods are required for their purification and analysis. Chemical derivatization strategies have been devised for enriching phosphoproteins and phosphopeptides. In this report, we employed a strategy that replaces the phosphate moieties on serine and threonine residues with a biotin-containing tag via a series of chemical reactions. Ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO)-depleted protein extracts prepared from Arabidopsis seedlings were chemically modified for 'biotin-tagging'. The biotinylated (previously phosphorylated) proteins were then selectively isolated by avidin-biotin affinity chromatography, followed by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). This led to the identification of 31 protein spots, representing 18 different proteins, which are implicated in a variety of cellular processes. Despite its current technical limitations, with further improvements in tools and techniques this strategy may be developed into a useful approach.

• 대한전기학회논문지
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• 제43권6호
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• pp.1027-1035
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• 1994

This paper aims to make an artificial arm control strategy. For this, we propose a new feature extraction method and design artificial neural network for the functional classification of myoelectric signal(MES). We first transform the two channel myoelectric signals (MES) for biceps and triceps into frequency domain using fast Fourier transform (FFT). And features were obtained by comparing the magnitudes of ensemble spectrum data and used as inputs to the three-layer neural network for the learning. By changing the number of units in hidden layer of neural network we observed the improvement of classification performance. To observe the effeciency of the proposed scheme we performed experiments for classification of six arm functions to the three subjects. And we obtained on average 94[%] the ratio of classification.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2005년도 전력전자학술대회 논문집
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• pp.408-410
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• 2005

Multilevel converters have emerged like a new strategy for energy conversion from medium power to high power. The main characteristic of the topologies classified as multilevel, is the use of commutation devices connected in series, allowing the distribution of the voltage and reducing stress in the commutation switches. Stacked Multicell Converter (SMC), is classified as single-phase voltage source inverter(VSI). Due to the fact, the SMC generates a signal of alternating current of several levels of voltage of direct current. The following work will demonstrate the flexibility of the above mentioned topology using a low cost control circuit architecture.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2008년도 추계학술대회 논문집
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• pp.16-18
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• 2008

A novel general direct duty ratio pulse width modulation strategy is proposed to modulate matrix converters. By using average concept over one switching period, the modulation algorithm and the required equations are derived to synthesize the desired output voltage and to achieve the controlled input power factor. The proposed method use continuous carrier and the predetermined duty ratio signal for directly generating gating signals an thus is named "direct duty ratio PWM(DDPWM)". The feasibility and validity of the proposed strategy are verified by experimental results.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2003년도 학술회의 논문집 정보 및 제어부문 B
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• pp.357-360
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• 2003

Improvement is required to solve problems depending on long term use of signal equipment in the existing city railroad and to raise quality of passenger services. Accordingly, for more efficient improvement, we examined the procedure of executing construction of the communication based train control system in Korean city railroads, which is globally studied. The procedure of executing construction of the system is to present a plan for construction first of ail and then to prepare a strategy for construction of the system. It is required to establish a procedure for execution in each step depending on the prepared strategy and then to establish a detailed execution plan. We studied such an execution procedure, divided the execution procedure for application of the train control system through five steps to prepare the execution procedure.