• Biotechnology and Bioprocess Engineering:BBE
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• 제11권5호
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• pp.442-448
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• 2006

Human lactoferrin (hLf) is an iron-binding glycoprotein that has been considered to play many biological roles in the human, including the stimulation of the immune system, antimicrobial and anti-inflammatory effects, and regulation of iron absorption. We generated transgenic Siberian ginseng (Acanthopanax senticosus) cell cultures producing a functional hLf protein using the signal peptide sequence from the endoplasmic reticulum and driven by an oxidative stress-inducible SWPA2 promoter which is highly expressed in plant cell cultures. The production of hLf increased proportionally to cell growth and showed a maximal level (up to 3.6% of total soluble protein) at the stationary phase in suspension cultures. Full-length hLf protein was identified by immunoblot analysis in transgenic cell cultures of Siberian ginseng. Recombinant hLf (rhLf) was purified from suspension cells of Siberian ginseng by ammonium sulfate precipitation, cation-exchange and gel filtration chromatography. N-terminal sequences of rhLf were identical to native hLf (nhLf). The overall monosaccharide composition of rhLf showed the presence of plant specific xylose while sialic acid is absent. Antibacterial activity of purified rhLf was higher than that of nhLf. Taken together, we anticipate that medicinal Siberian ginseng cultured cells, as demonstrated by this study, will be a biotechnologically useful source for commercial production of functional hLf not requiring further purification.

• Parasites, Hosts and Diseases
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• 제57권6호
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• pp.567-573
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• 2019

An archaeoparasitological analysis of the soil samples from Nadym Gorodok site of Western Siberia has been carried out in this study. The archaeological site was dated as the 13 to 18th century, being characterized as permafrost region ensuring good preservation of ancient parasite eggs. Parasite eggs as Opisthorchis felineus, Alaria alata, and Diphyllobothrium sp. were found in the archaeological soil samples, which made clear about the detailed aspects of Nadym Gorodok people's life. We found the Diphyllobothrium sp. eggs throughout the 14 to 18th century specimens, allowing us to presume that raw or undercooked fish might have been commonly used for the foods of Nadym Gorodok inhabitants and their dogs for at least the past 400 years. Our study on Nadym Gorodok specimens also demonstrate that there might have been migratory interactions and strong economic ties between the people and society in Western Siberia, based on archaeoparasitological results of Opisthorchis felineus in Western Siberia.

• 한국산림과학회지
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• 제108권1호
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• pp.133-137
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• 2019

하늘다람쥐의 형태적 특성을 파악하기 위해 강원도 원주시 백운산에서 2014년 4월부터 2016년 3월까지 하늘다람쥐의 6가지 외부형태적 특성에 대한 측정을 실시하고 분석하였다. 그 결과 하늘다람쥐는 암컷이 수컷보다 큰 암컷편향적 성적이형성을 나타내는 것으로 확인되었다. 이는 단독 생활을 하고 암컷 혼자 새끼를 키우는 하늘다람쥐의 번식전략과 관련이 있는 것으로 보인다. 국내와 핀란드, 일본에서 파악된 하늘다람쥐의 외부형태 측정치 중 체중과 머리-몸통의 길이를 비교한 결과, 모두 핀란드와 일본 그리고 한국 순서대로 위도가 높은 지역이 크게 나타났다. 이러한 형태적 특성의 차이는 분포 지역 위도에 따른 기후와 서식환경 차이에 따른 것으로 생각되며, 추후 체중과 머리-몸통의 길이 이외의 외부형태 측정치에 대한 비교 연구가 필요할 것으로 생각된다.

• 한국임상수의학회지
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• 제21권3호
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• pp.314-318
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• 2004

A 7-month-old 8.2 kg female Siberian Husky with history of exercise intolerance, delayed growth, and tachypnea was presented to Veterinary Medical Teaching Hospital, Kyungpook National University. A holosystolic murmur was identified in cardiac ausculation. In electrocardiography, thoracic radiographs, and ultrasonography, a right axis deviation, enlarged right ventricle, stenotic main pulmonary artery, large ventricular septal defect, marked right ventricle hypertrophy, and overriding aorta with dilation were identified. The color Doppler examination showed the left-to-right shunting of blood via interventricular septal defect and the blood flow streams converging from the right and left ventricles into the aorta. The dog was diagnosed as tetralogy of Fallot. The current state of the dog is well-tolerated without any specific medication.

• Bulletin of the Korean Chemical Society
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• 제35권10호
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• pp.2891-2894
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• 2014

The effects of mechanical activation of carbon black on the processing and properties of butadiene nitrile rubber were studied. Mechanical activation of carbon black caused an improvement in the physical and mechanical properties of the butadiene-nitrile rubber, BNR-18AMN. The optimum activation time that would afford rubber with improved properties was established.

• 한국임상수의학회지
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• 제15권1호
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• pp.171-173
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• 1998

A case of peritonitis caused by Actinomycotic spp is reported in a 12-year-old male Siberian tiger. Grossly, the mesentery was markedly thickened and contained numerous 1 to 3 mm diameter, white to yellowish foci. Fibrous adhesion showing tumorous thickening was also noted between the mesentery and abdominal organs. Histologicallyi the thickened mesentery and masses consisted of necrotic center with bacterial colonies surrounded by eosinophilic club (Splendore-hoeppli), neutrophils, macrophages, a few Iymphocytes and fibrosis. The bacterial colonies stained positvely with Gram's stain but were negative on acid-fast and periodic acid-Schifr method. Howeverr since the bacterial culture was not availablei the definitive causative agent was not able to specified.

• Animal cells and systems
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• 제12권4호
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• pp.269-277
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• 2008

Siberian flying squirrel, an endangered species in South Korea, is distributed through major mountain regions of South Korea. The number of Siberian flying squirrel(Pteromys volans) in South Korea has decreased and their habitats are fragmented and isolated because of anthropogenic activities. So far no molecular genetic data has, however, been available for their conservation and management. To obtain better information concerning genetic diversity and phylogenetic relationships of the Siberian flying squirrel in South Korea, we examined 14 individuals from South Korea, 7 individuals from Russia, and 5 individuals from northeastern China along with previously published 29 haplotypes for 1,140 bp of the mtDNA cytochrome b gene. The 14 new individuals from South Korea had 7 haplotypes which were not observed in the regions of Russia and Hokkaido. The level of genetic diversity(0.616%) in the South Korean population was lower than that in eastern Russia(0.950%). The geographical distribution of mtDNA haplotypes and reduced median network confirmed that there are three major lineages of Siberian flying squirrel, occupying; Far Eastern, northern Eurasia, and the island of Hokkaido. The South Korean population only slightly distinct from the Eurasia, and eastern Russian population, and is part of the lineage Far Eastern. Based on these, we suggest that the South Korean population could be considered to belong to one partial ESU(Far Eastern) of three partial ESUs but a different management unit. However, the conservation priorities should be reconfirmed by nuclear genetic marker and ecological data.

• 한국광물학회지
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• 제30권4호
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• pp.219-227
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• 2017

북극해 망간단괴는 카라해, 바렌츠해 등에서 발견되었지만, 광물조성과 화학조성에 대해서는 정확하게 조사된 바가 없다. 이번 연구에서는 Arctic Expedition ARA07C 탐사를 통해 동시베리아 북부 해역에서 획득한 망간단괴에 대하여, 광물학적 및 지구화학적 특성을 파악하고, 이를 통하여 그 성인에 대하여 추정하였다. 망간단괴를 구성하는 주된 산화망간광물은 부서라이트, 버네사이트, 버나다이트 등이다. 망간단괴는 대체로 방사상대와 괴상대 조직을 나타내며, 층상대는 제한적으로 발달한다. 동시베리아해 망간단괴의 주요한 특징인 방사상대에는 주로 첨-구상조직이 발달한다. 동시베리아해 망간단괴는 태평양이나 인도양에서 산출되는 망간단괴에 비하여 Mn은 풍부하지만 Fe의 함량은 매우 부족하다. 단괴 외곽과 내부, 또는 미세조직에 따른 화학성분의 차이는 거의 없었다. 미세조직 간의 화학적 성분 차이가 거의 나타나지 않기 때문에 단괴는 성장하는 동안 하나의 성인을 가질 가능성이 크며, $Mn-Fe-(Cu+Ni+Co){\times}10$ 삼각도표에서 모두 속성기원을 지시한다. 북극해로 유입되는 대부분의 Mn은 강 또는 연안 침식에 의해 비롯되며, 이들 대부분이 북극해에 갇혀져 있기 때문에 동시베리아해 망간단괴에서 특징적으로 높은 Mn 함량을 나타내는 것으로 판단된다.

• Animal cells and systems
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• 제15권2호
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• pp.161-168
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• 2011

Siberian chipmunks, Tamias sibiricus, are one of several popular companion animals found in the pet shops of South Korea. At present, however, there have been no studies done in South Korea examining their origin even though they could be potential carriers of zoonotic diseases, and are a species of concern for efficient conservation and management strategies. Sequences of the mitochondrial cytochrome b gene (1140 bp) were determined to investigate the origin of Siberian chipmunks sold in four South Korean pet shops through comparison with sequence data from animals of known locality. Nine Siberian chipmunks were collected from pet shops in South Korea, which resulted in nine haplotypes. One (AR) of these coincided with the haplotype previously described. Phylogenetic and network analyses using 53 haplotypes including 45 haplotypes from GenBank showed three phylogenetic groups in South Korea, almost concordant to locality, designated as northern, central, and southern parts as described in a previous study. Of the nine individuals examined from the pet shops, eight were clustered into the northern phylogroup but one (cgrb9153) was grouped with the southern phylogroup, implying that at least the Siberian chipmunks examined in this study did not originate from other countries. It is likely that most individuals sold in the pet shops of Seoul were caught in the wild in Gyeonggi-do and Gangwon-do, or are maternal descendants of captive-bred individuals originating from the northern part of South Korea. It is recommended that conservation and management units of Korean chipmunks should be examined in further detail.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2010년도 춘계학술대회 논문집
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• pp.656-663
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• 2010

본 연구에서는 K.Terzaghi의 유효응력이론을 인용하여 압밀기반의 안전하중 산출 과정과 결과를 제시하였다. 일반적인 강도를 평가하기 위하여 이용된 J.I.Solovyov의 순간강도 이론에 기초를 두어, 제시한 방법은 포화된 연약점토지반에서 도로의 유지보수 뿐 아니라 축조 과정에 안전하중의 계산 적용이 가능하다. 실제로 편리한 표준 압밀 drain test에서 정의된 점착력, 내부 마찰각과 같은 강도정수를 가진 유효응력은 실제적 적용을 고려하기 위하여 산출된다.