• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.335-339
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• 2003

Several experiments were carried out to transfer LEAFY gene to Dendranthema grandiflora 'Shuho-no-chikara' by Agrobacterium LBA4404 carrying pSK109 encoding LEAFY gene. Kanamycin 10mg/L was used in first selection medium, and 20mg/L in the second one. Co-culture for 3 days was more effective in increasing transformation efficiency than that for 7 days. The transformation efficiency by Agrobacterium LBA4404 carrying pSK109 encoding LEAFY gene was about 2.8％ until the second selection, but only 0.13％ of shoots (two plants) was confirmed as a transgenic plants in Southern analysis. The escape of putative transformants was occured seriously in the process of selections, PCR analysis for confirming of neomycin phosphotransferaseII (npt II), and Southern analysis for LEAFY gene. One transgenic plant appeared 7 days'early flowering in field.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.10a
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• pp.170-170
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• 2004

• Journal of Plant Biotechnology
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• v.36 no.4
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• pp.373-383
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• 2009

To set efficient transformation system in chrysanthemum, thirty-four chrysanthemum (Dendranthema grandiflorum Kitamura) varieties were collected and cultured for shoot regeneration. Five varieties, ‘Shuho-no-chikara', ‘Zinba', ‘Baekma', ‘Pink pride' and ‘Keumsu' of them were selected, because they had a high shoot regeneration efficiency. MS medium containing 1.0 mg/L NAA and BA respectively was very adequate for shoot regeneration in those varieties. MS medium with 3.0 mg/L NAA and 1.0 mg/L kinetin in ‘Shuho-no-chikara' and the medium with 0.5 mg/L NAA and 3.0 mg/L BA in ‘Keumsu' were also suitable for shoot regeneration. The most efficient callus induction and shoot regeneration were obtained on MS medium. Shoot regeneration was enhanced more than 8% on MS medium with 0.3% phytagel and 10-15 mg/L putrescine. The best cultural material for shoot regeneration was stem. When stem was used as a culture material, shoot regeneration rate was increased more than 26% and the days to shoot regeneration was shortened about 14 days.

• Journal of Multimedia Information System
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• v.9 no.2
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• pp.161-170
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• 2022

Under General anesthesia with isoflurane, we insert a chicken's esophageal catheter into the near the left atrium. 1MHz radio wave was added to electrocardiogram electrodes of the esophagus, and the change of impedance (phase) was obtained by amplitude synchronous detection technique. At the same time, a thin tube is surgically inserted into the axillary artery to continuously measure blood pressure. The correlation between impedance (phase) and blood pressure was obtained. Both showed a very high correlation (R²=0.9665). It was also observed the waveform flowing from the left atrium into the left ventricle. When an individual infected with the avian influenza virus develops, the cytokine storms lead to hypotension earlier than the test for antigen-antibody reaction. In order to detect this, in the future, this impedance technique will be useful for screening individuals infected with avian influenza virus by measuring the blood pressure of chickens in cages in a non-contact manner using microwaves.

• Korean Journal of Applied Biomechanics
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• v.33 no.4
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• pp.119-127
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• 2023

Objective: This study aimed to compare the activity of the trunk and leg muscles while performing fente (in fencing) wearing weighted and waterbag vests. Method: The electromyography test was used to measure and analyze the activation of the trunk and leg muscles. Eight active fencers from B University (age: 19.5 ± 0.66 years, height: 179.75 ± 5.93 cm, weight: 72 ± 6.32 kg) were selected for this study. Results: According to the EMG analysis results of the 4 muscles measured in this study, left-right differences were observed for rectus abdominis and external oblique abdominis, but left-right differences between the groups were not significant. The gluteus medius muscle was not significantly different from the adductor muscle, but there were significant differences between the groups. Conclusion: The electromyographic analysis of the four muscles measured in this study revealed no significant difference between the left and right recti abdominis and external obliques depending on the vests. However, significant differences were observed between the left and right gluteus medius and adductor longus. Our results can be interpreted as the effects of the inherent movements involved in the fente. Furthermore, our results indicate that the weight transfer while wearing a waterbag vest, which provides an unstable environment, increased the activity of leg muscles.

• Journal of Life Science
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• v.16 no.5
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• pp.711-715
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• 2006

The objective of this study was focused on the enhancement of pollen germination frequency in peach (Prunus $persica\;_{SIEB}$) under low temperature conditions. The effect of factors such as amino acid, polyamine, and flavonoid on the pollen germination was investigated, and the results are summarized as follows. When amino acid, polyamine or flavonoid was added to the germination medium at $10^{\circ}C$, pollen germination frequency was strongly promoted. Optimum concentration of each supplement for pollen germination enhancement was $100\;mg{\cdot}L^{-1}\;H_3BO_3$, 10 mM asparagine, 10 mM glutamine, 100 mM spermine, $1000\;{\mu}M$ putrescine, and $1.0\;{\mu}M$ kaemferol. The best combination of factors in pollen germination was $100\;mg{\cdot}L^{-1}\;H_3BO_3+10\;mM$ asparagine, followed by $100\;mg{\cdot}L^{-1}\;H_3BO_310mM$ glutamine, $100\;mg{\cdot}L^{-1}\;H_3BO_3+200mM$ spermine, and 10 mM asparagine. These combinations promoted pollen germination by 18% in 'Nagasawa-Hakuho', and 19% in 'Shuho' compared to their germination percentage on the basal medium.

• Journal of Life Science
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• v.16 no.4
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• pp.561-565
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• 2006

This study was carried out to identify the effect of sucrose, calcium, and boron on the increase of pollen germination frequency in peach $(Prunus\;persica\;_{SIEB})$ under various temperature levels. The highest pollen germination was obtained at $20^{\circ}C$. Pollen germination was completed in 3.5 hrs. after planting of pollen on the basal medium which was composed of 1% agar and 10% sucrose, and conditioned pH 5.5. The optimum concentrations of sucrose and $H_3BO_3$ at $25^{\circ}C$ were 10% and $100\;mg\;{\cdot}\;L^{-1}$, respectively. However, $CaCl_2$ inhibited pollen germination in peach. Eight peach cultivars showed significantly different germinabilities at $10^{\circ}C$ on the basal medium. 'Nagasawa-Hakuho' showed the highest germinability of 26.1%, and 'Baekmijosaeng' showed the lowest germinability of 0.1%. $H_3BO_3$ strongly promoted pollen germination frequency when added to the germination medium at $10^{\circ}C$. Optimum concentration was $100\;mg\;{\cdot}\;L^{-1}$.