Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Sucrose, Calcium and Boron Added in the Medium on Pollen Germination of Peach(Prunus persica SIEB)

Sucrose, 칼슘, 붕소 첨가가 복숭아 화분 발아에 미치는 영향

  • Cheon, Beong-Duck (Ansong City Office of Agricultural Development and Technology Center) ;
  • Choi, In-Soo (School of Bio-Resources, Pusan National University) ;
  • Kang, Jum-Soon (School of Bio-Resources, Pusan National University)
  • 천병덕 (안성시 농업기술센타) ;
  • 최인수 (부산대학교 생명자원과학부) ;
  • 강점순 (부산대학교 생명자원과학부)
  • Published : 2006.07.31
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Abstract

This study was carried out to identify the effect of sucrose, calcium, and boron on the increase of pollen germination frequency in peach $(Prunus\;persica\;_{SIEB})$ under various temperature levels. The highest pollen germination was obtained at $20^{\circ}C$. Pollen germination was completed in 3.5 hrs. after planting of pollen on the basal medium which was composed of 1% agar and 10% sucrose, and conditioned pH 5.5. The optimum concentrations of sucrose and $H_3BO_3$ at $25^{\circ}C$ were 10% and $100\;mg\;{\cdot}\;L^{-1}$, respectively. However, $CaCl_2$ inhibited pollen germination in peach. Eight peach cultivars showed significantly different germinabilities at $10^{\circ}C$ on the basal medium. 'Nagasawa-Hakuho' showed the highest germinability of 26.1%, and 'Baekmijosaeng' showed the lowest germinability of 0.1%. $H_3BO_3$ strongly promoted pollen germination frequency when added to the germination medium at $10^{\circ}C$. Optimum concentration was $100\;mg\;{\cdot}\;L^{-1}$.

우리나라 과수 중 개화기가 빠른 복숭아는 저온에 의하여 화분발아 장해로 결실율이 저하되고 있다. 본 연구는 저온에서 화분 발아율을 증진시킬 수 있는 방법을 강구하기 위한 기초연구로서 화분 발아조건과 배지내에 sucrose, $CaCl_2$ 및 $H_3BO_3$ 첨가농도를 구명하고자 일련의 실험을 실시하였다. 복숭아 화분은 배지에 치상한 후 30분부터 발아가 시작되어 3시간 30분까지 지속되었으나, 그 이후는 더 이상 발아되지 않았다. 따라서 복숭아의 화분발아 판정을 위한 적정 시간은 치상 후 4시간이 좋았다. 복숭아 화분의 적정발아 온도는 $20^{\circ}C$였고, 이보다 저온이거나 고온에서는 화분발아율이 감소하였다. Sucrose는 화분발아를 촉진하였고 최적농도는 10%였다. 무기염류 중 $H_3BO_3$ 첨가는 '장택백봉'과 '수봉' 품종에서 화분 발아율을 무처리에 비해 $20{\sim}30%$ 증진시켰으며, 그 효과는 저온인 $10^{\circ}C$에서 현저하였다. 최적처리 농도는 $100\;mg\;{\cdot}\;L^{-1}$ 처리였다. 반면 $CaCl_2$ 첨가하면 화분발아율이 감소하였다. $10^{\circ}C$의 저온에서 화분발아율은 품종간에 뚜렷한 차이가 있었으며, '장택백봉', '백봉', '월미', '백도', '대구보', '유명', '수봉', '백미조생'의 순으로 화분발아율이 낮았다.

Keywords

References

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