Journal of Plant Biotechnology

  • 제36권4호
  • /
  • Pages.373-383
  • /
  • 2009
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

형질전환 효율성 향상을 위한 국화 최적 품종 선발 및 배지.배양조건설정

Selection of the fittest varieties of chrysanthemum (Dendranthema grandiflorum Kitamura) and set of culture condition for efficient transformation

  • 발행 : 2009.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

To set efficient transformation system in chrysanthemum, thirty-four chrysanthemum (Dendranthema grandiflorum Kitamura) varieties were collected and cultured for shoot regeneration. Five varieties, ‘Shuho-no-chikara', ‘Zinba', ‘Baekma', ‘Pink pride' and ‘Keumsu' of them were selected, because they had a high shoot regeneration efficiency. MS medium containing 1.0 mg/L NAA and BA respectively was very adequate for shoot regeneration in those varieties. MS medium with 3.0 mg/L NAA and 1.0 mg/L kinetin in ‘Shuho-no-chikara' and the medium with 0.5 mg/L NAA and 3.0 mg/L BA in ‘Keumsu' were also suitable for shoot regeneration. The most efficient callus induction and shoot regeneration were obtained on MS medium. Shoot regeneration was enhanced more than 8% on MS medium with 0.3% phytagel and 10-15 mg/L putrescine. The best cultural material for shoot regeneration was stem. When stem was used as a culture material, shoot regeneration rate was increased more than 26% and the days to shoot regeneration was shortened about 14 days.

키워드

참고문헌

  1. Ammirato PV (1983) Embryogenesis In Da Evans, Sharp WR, Ammirato PV, Yamada Y(eds), Handbook of Plant Cell Culture Vol. 1 pp 82-123. Techniques for propagation and breeding, Macmillan Publishing co. New york
  2. Brar MS, Moore MJ, Al-KhayriJ M, Morelock TE, Anderson EJ (1999) Ethylene inhibitors promote in Vitro regeneration of cowpea (Vigna unguiculata L.). In Vitro Cellular and Develop. Biol. Plant 35
  3. Bhojwani SS, Razdan MK, Choi SJ (1989) Plant tissue culture. Kore text cooperation pp 33-49, pp 118-119
  4. Carla EV, Schaik A, Posthuma MJ, De Jeu, Jacobsen E (1996) Plant regeneration through somatic embryogenesis from callus induction immature embryos of Alstromeria spp. L. Plant Cell Report 15:377-380 https://doi.org/10.1007/BF00232375
  5. Choi YE, Yeo UD, Soh WY (1986) Somatic embryogenesis and plantlet regeneration from stem callus of Cedrala sinensis Juss. Korean J. Plant Tissue Culture 13:61-70
  6. Chung JD, Harn JS, Sohn JK (1995) Somatic Embryogenesis from Filament-Derived Callus of Paeonia lactiflora PALL. Korean J. Plant Tissue Culture 22:47-52
  7. Cogner BV, Hanning GE, Mcdaniel DJ (1983) Direct embryogenesis from mesopphyll cells of orchardgrass. Science 221:850-851 https://doi.org/10.1126/science.221.4613.850
  8. Lee JH, Lee SY (1995) Effects of Gelling Agents and Growth Regulation on Rice Anther Culture. Korean J. Plant Tissue Culture 22: 35-40
  9. Lee JH, Lee SY, Namkoong SB (1996) Effects of Growth Regulators, Sucrose and Gelling Agents on Callus Growth and Plant Regeneration in Angelica koreana MAX. Korean J. Medicinal crop sci. 4: 78-85
  10. Ferrer R, Mignon G, Litvey J (1984) Arginine decarboxylase and polyamines required for embryogenesis in the wild carrots. Science 223: 1433-1435 https://doi.org/10.1126/science.223.4643.1433
  11. Hakan T (2004) The effect of nitrate(ethylene inhibitor) on in vitro shoot development in potato (Solanum tuberosum L). Biotechnology 3: 72-74 https://doi.org/10.3923/biotech.2004.72.74
  12. Han BH, Suh EJ, Lee SY, Shin HK, Lim YP (2007) Selection of nonbranching lines induced by introducing Ls-like cDNA into chrysanthemum (Dendranthema × grandiflorum (Ramat.) Kitamura) “Shuho-no-chikara”. Scientia Horticulturae 115:70-75 https://doi.org/10.1126/science.221.4613.850
  13. Hodges TK, Kamo KK, Imbrie CW, Becwar MR (1986) Genotype specificity of somatic embryogenesis and regeneration in maize. Bio. Tech. 4:219-223 https://doi.org/10.1038/nbt0386-219
  14. Hyde CL and Philips GC (1996) Silver nitrate promotes shoot development and plant regeneration of chile pepper (Capsicum annunm L.) via organogenesis. L. In Vitro Cellular and Develop. Biol. Plant 32:72-80
  15. Kim SH, Kim MW, Kang YH, Lee SH (1993) Changes in endogenous polyamine levels during adventitious root regeneration from populus leaf segments. Korean J. Plant Tissue Culture 20:329-335
  16. Korner RN and Nataraja K (1965) Experimental studies in Ranunculus sceleratus L. development of embryos from the stem epidermis. Phytomorphology 15: 132-137
  17. Kubo T, Tsuro M, Tsukimori A, Shizukawa Y, Takemoto T, Inaba K, Shiozaki S (2006) Morphological and physiological changes in transgenic chrysanthemum morifolium Ramat. 'Oguranishiki'with rolC. Journal of the Japanese Society for Horticultural Science 75: 312-317 https://doi.org/10.2503/jjshs.75.312
  18. Levi A, Sink KC (1991) Somatic embryogenesis in Asparagus; The role of explants and growth regulators. Plant Cell Report 10:71-75 https://doi.org/10.1007/BF00236460
  19. Linsmaier EM and Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18:100-127 https://doi.org/10.1111/j.1399-3054.1965.tb06874.x
  20. Liu LF and Lai KL (1985) High frequency plant regeneration from water stressed rice tissue culture. In abstracts of the first international congress of plant molecular biology. p 11
  21. Liu LF and Lai KL (1986) Further studies on the variability of plant regeneration from young embryos callus culture in rice plant (Orisa sativa L.). Jpn J Crop Sci. 55:41-46 https://doi.org/10.1626/jcs.55.41
  22. Mitiouchkina TY, Dolgov SV (2000) Modification of chrysanthemum plant and flower architecture by rol C gene from Agrobacterium rhizogenesis induction. Acta Hort 508:163-169
  23. Mohiuddin AKM, Choedhury MKU, Abdullah ZC, Napis S (1997) Influence of silver nitrate (ethylene inhibitor) on cucumber in vitro shoot regeneration. Plant Cell Tissue, Organ Culture 51:75-78 https://doi.org/10.1023/A:1005814514409
  24. Moon HK, Hong YP, Kim YW, Lee JS (2001) Genotype Effect on Somatic Embryogenesis and Plant Regeneration of 15 Aralia elata. Korean J. Plant Tissue Culture 28:129-134
  25. Moon HP, Choi SH, Soh YH (1983) Effect of high agar medium on plant regeneration in rice anther culture. Kor. J. Breed 20:335-340
  26. Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  27. Paek KY, Cho JT (1994) Year-round production system of pathogen-free stock and micropropagation in Fritillaria thunbergii. Korea RDA crop experiment station. pp 11-12
  28. Ryu JH, Choo BG, DOoo HS, Kwon TH (1996) Plant Regeneration by the Stem Culture in Houttuynia cordata Thunberg. Korean J. Medicinal crop sci. 4:126-131
  29. Sakurai N and Masuda Y (1977) Effect of indole-3-acetic acid on cell wall loosening ; Changes in mechanical properties and noncellulosic glucose content of Avenacoleoptile cell wall. Plant Cell Physiol. 18: 587-594
  30. Sharp WR, Sondahl MR, Caldas LS, Maraffa SF (1980) The physiology of asexual embryogenesis. In : Horticultural Reviews II, AVI Publishing Company, Westport
  31. Smith DL and krikorian AD (1990) Somatic preembryos production from excised wounded zygotic carrot embryos on hormone-free medium evaluation of the effects of pH, ethylene and activated charcol. Plant Cell Rep. 9:34-37
  32. Song JJ, Kim MW, Lee SH, Kang BG, Kang YH (1993) Mechanism of polar differentiation of poplar; Endogenous hormone content and effect of NAA and polyamine on poplar differentiation. Korean J. Plant Tissue Culture 20: 21-26
  33. Sung NS, Paek KY, Chae YA (1994) Micropropagation of Aconitum carmichaeli Debx. and Rehmania glutinosa Libosch. through the plant tissue culture techniques. Korea RDA crop experiment station. pp33-34
  34. Bhojwani SS, Razdan MK, Choi SJ (1989) Plant tissue culture. Kore text cooperation pp33-49, pp 118-119
  35. Takatsu Y, Nishizawa Y, Hibi T, Akutsu T (1999) Transgenic chrysanthemum (Dendrantherma grandiflorum) expressing a rice chitinase gene shows enhanced resistance to gray mold (Botrytis cineria). Sci. Hort. 82:113-123 https://doi.org/10.1016/S0304-4238(99)00034-5
  36. Takayama S, and Misawa M (1980) Differentiation in lillium bulb scales grown in vitro. Effect of activated charcol. physiological age of bulbs and sucrose concentration on differentiation and scale leaf formation in vitro. Physiol Plant 48:121-125 https://doi.org/10.1111/j.1399-3054.1980.tb03229.x
  37. Van Eck and Kitto SL (1992) Regeneration of peppermint and orangemint from leaf discs. Plant Cell, Tissue, Organ Culture 30:41-49 https://doi.org/10.1007/BF00039999
  38. Vogelezang JL, Cujipers M, Th de Graaf-Van der Zande (1992) Growth regulation of breeding plants by reversed day/night temperature. Acta Hort. 305:37-43
  39. Wetherel DF and DougallK (1976) Source of nitrogen supporting growth and embryogenesis in cultured wild carrot tissue. Physiol Plant 37:97-103 https://doi.org/10.1111/j.1399-3054.1976.tb03939.x
  40. Williames EG and Maheswaran G (1986) Somatic embry ogenesis; Factors influcing coordinated behavior of cells on embryogenic group. Ann. Bot. 57:443-462 https://doi.org/10.1093/oxfordjournals.aob.a087127