• Journal of Bio-Environment Control
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• v.32 no.3
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• pp.242-248
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• 2023

This study was conducted to evaluate the effects of trinexapac-ethyl (TE) and prohexadione-calcium (PC) on the growth and turf quality of perennial ryegrass (Lolium perenne L.). Treatments were designed as follows; control (non-treatment), TE (TE 0.01 a.i. g·m^-2·100mL^-1), and PC (PC 0.01 a.i. g·m^-2·100mL^-1). Compared with control, turf color index and chlorophyll content of TE and PC treatments were not significantly different. As applied plant growth regulators (PGRs), shoot length in the TE and PC was decreased by 8.9-12.4% and 6.7-13.6%, respectively, and clipping yield by 44.4-45.8% and 40.6-40.9%. When evaluated with the growth of shoot length after applying PGRs, residual days of TE and PC were 43.6 and 37.9 days after treatment of PGRs (DAT) in the 1st experiment (July 29-August 26), respectively, and 38.3 and 39.5 DATs in the 2nd experiment (September 30-October 28). These results indicated that an application of TE or PC decreased shoot length and clipping yield in the perennial ryegrass, and their residual time was about 40±3 DATs. Perennial ryegrass applied with PGRs like TE and PC was not significant seasonal difference, and might be not phytotoxic.

• Korean Journal of Environmental Agriculture
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• v.42 no.4
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• pp.389-395
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• 2023

This study evaluated the effects of gibberellic acid (GA₃) on the growth and quality of creeping bentgrass (Agrostis palustris Huds.). Experimental treatments included a No application of fertilizer and GA₃ (NFG) Control [3 N active ingredient (a.i.) g/m²], 0.3GA₃ (GA₃ 0.3 a.i. mg/m²/200 mL), 0.6GA₃ (GA₃ 0.6 a.i. mg/m²/200 mL), 1.2GA₃ (GA₃ 1.2 a.i. mg/m²/200 mL), and 2.4GA₃ (GA₃ 2.4 a.i. mg/m²/200 mL). Additionally, the study included a 1.5N+GA₃ experiment with similar GA₃ treatments combined with 1.5N a.i. g/m² : NFG, Control (3N a.i. g/m²), 1.5N+ 0.3GA₃ (1.5N a.i. g/m²+GA₃ 0.3 a.i. mg/m²/200 mL), 1.5N+0.6GA₃ (1.5N a.i. g/m²+GA₃ 0.6 a.i. mg/m²/200 mL), 1.5N+1.2GA₃ (1.5N a.i. g/m²+GA₃ 1.2 a.i. mg/m²/ 200 mL), and 1.5N+2.4GA₃ (1.5N a.i. g/m²+GA₃ 2.4 a.i. mg/m²/200 mL). Compared to the NFG, turf color index chlorophyll content was not significantly different (p< 0.05). However, shoot length in 1.2GA₃, 2.4GA₃, 1.5N+0.3GA₃, 1.5N+0.6GA₃, 1.5N+1.2GA₃, and 1.5N+2.4GA₃ treatments increased by 0.8%, 10.6%, 5.15%, 8.3%, 13.5 %, and 21.6%, respectively, compared to the control. As compared to the control, clipping yield in 1.5N+1.2GA₃ and 1.5N+2.4GA₃ treatments increased by 7.1% and 14.3 %, respectively. These results indicated that GA₃ application increased shoot length, with the 1.2GA₃ treatment showing shoot length similar to the control (3N a.i. g /m² ).

• Journal of Plant Biotechnology
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• v.7 no.4
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• pp.247-257
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• 2005

An efficient, rapid and large-scale in vitro clonal propagation of agronomically important Indian cereal crop genotypes (NSH27 & K5) of Sorghum bicolor (L.) Moench. by enhanced shoot proliferation in shoot tip segments was designed. MS medium fortified with plant growth regulators and coconut water markedly influenced in vitro propagation of Sorghum bicolor. In vitro plantlet production system has been investigated on Murashige and Skoog (MS) medium with the synergistic combination of 6-benzyladenine ($22.2\;{\mu}M$), kinetin ($4.6\;{\mu}M$), adenine sulphate ($2.8\;{\mu}M$), 5% coconut water and 3% sucrose which promoted the maximum number of shoots as well as beneficial shoot length. Subculturing of shoot tip segments on a similar medium enabled continuous production of more than 100 healthy shoots with similar frequency. When the healthy shoot clumps were cultured on MS medium fortified with 6-benzyladenine ($22.2\;{\mu}M$), kinetin ($4.6\;{\mu}M$), adenine sulphate ($2.8\;{\mu}M$), ${\alpha}$-naphthaleneacetic acid ($2.7\;{\mu}M$), ascorbic acid ($30.0\;{\mu}M$) and 5% coconut water, a rapid production of axillary and adventitious buds was developed after 8 wk culture. More than 300 shoots were produced 10 wk after culture. Rooting was highest (100%) on half strength MS medium containing 22.8 mM IAA. Micropropagated plants established in garden soil, farmyard soil and sand (2:1:1) were uniform and identical to the donor plant with respect to growth characteristics. These plants grew normally without showing any traits.

• Journal of Plant Biotechnology
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• v.47 no.1
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• pp.40-45
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• 2020

An efficient protocol for multiple shoot induction and plant regeneration from axillary bud culture of Magnolia 'Vulcan' was developed in the present study. Primary shoots were obtained from axillary bud explants cultured on Murashige and Skoog (MS) medium containing 1.0 mg/L 6-benzylaminopurine (BA). To induce multiple shoots effectively, primary shoot tips were cultured on MS medium supplemented with different concentrations of BA and zeatin at 0, 0.2, 0.5, and 1.0 mg/L. Of these treatments, the MS medium with 0.5 mg/L BA resulted in the highest number of shoots per explant with an average value of 5.9, and it produced the greatest shoot height at 4.8 cm after 12 weeks of culturing. In the rooting of in vitro produced shoots, the greatest percentage of explants forming roots (91.3%), number of roots per explant (9.7), and root length (2.8 cm) were obtained in half-strength MS medium supplemented with 6.0 mg/L indole-3-butyric acid (IBA). Regenerated plantlets were successfully acclimatized and hardened off inside the culture room with 87.5% survival rate. Plants were transferred to a greenhouse with a 97.2% survival rate. The highly efficient shoot multiplication and plant regeneration system reported herein can be used for large-scale clonal propagation of valuable Magnolia species or cultivars.

• Journal of Korean Society of Forest Science
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• v.2 no.1
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• pp.48-51
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• 1962

1. The analysis of the shoot height growth of Phyllostachys edulis A. et C. Rivi$\acute{e}$re which stand is growing at the middle part of South Korea is presented in the present paper. The shoot height was carefully measured twice a day, 10 am and 4 pm. With this, six hours from 10 am to 4 pm is regarded as day-time-length and 18 hours of the rest as night-length. 2. The measurement were made in 1954. The very young shoots were classed into three diameter groups basing at the ground surface level to see the the afterwards influences of shoot size on height growth. 3. The hourly mean height growth in day time was higher than that in night time. The standard deviations and coefficients of variations of the daily growth of the shoot are presented in Figures 2 and 3 respectively. 4. The hourly mean height growth of the shoot by diameter groups and by day and night period are presented in Fig. 4 and 5 respectively. 5. The growth percentages of the shoot by diameter groups and by day and night is graphed in Fig. 6.

• Journal of Forest and Environmental Science
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• v.18 no.1
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• pp.1-6
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• 2001

This research was carried out to establish the large quantity propagation system for Kalopanax pictus using root cutting. The results are as follows. As there was no significant difference in the promotion of adventitious shoot development from the root cuttings of Kalopanax pictus among kinds (IBA, NAA and Kinetin) and concentrations (0 to 2000 mg/L) of growth regulators, no application is recommended, which showed more than 90% of shoot development. Horizontal and vertical placement of rooting showed more than 93% of shoot occurrence, but the latter showed more desirable growth traits in the number of shoot development, number of leaf, petiole length, and leaf length and leaf width Polarity was observed and thus normal vertical placement of root cutting is preferred. However, root cuttings placed upside down showed the shoot development at the distal end, which indicates further sophisticated test is necessary to investigate this phenomenon.

• FLOWER RESEARCH JOURNAL
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• v.18 no.2
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• pp.83-86
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• 2010

This study was conducted to develop in vitro propagation techniques of new cultivar, 'Greenstar', bred by Highland Agriculture Research Center. The multiple shoot induction and plant growth of in vitro plant were analyzed by MS media concentration (1/2 MS, 1 MS and 2 MS medium), plant growth regulators and its proper concentration; CPPU [Forchlorfenuron(N-(2-chloro-4-pridyl)-3-phenylurea) (0, 0.5, 1.0 and $2.0mg{\cdot}L^{-1}$), thidiazuron [(TDZ), (0.01, 0.1, 0.5 and $1.0mg{\cdot}L^{-1}$), zeatin (0, 0.5, 1.0 and $2.0mg{\cdot}L^{-1}$), and BA [6-benzylaminiopurine(BA), (0, 0.5, 1.0 and $2.0mg{\cdot}L^{-1}$)] in MS (3% sugar and 0.8% agar with pH 5.7) media. The highest number of induced shoots, leaves and roots were shown in 1/2 MS medium concentration. On the 1/2 MS medium, shoot numbers, shoot length, leaf numbers and root numbers were 11.0, 1.9 cm, 24.7, and 8.0, respectively. On the absence of CPPU in the 1/2 MS medium, shoot length and root numbers was greater than CPPU treatment, but the highest number of shoots was induced by the $2.0mg{\cdot}L^{-1}$ of CPPU concentration in 1/2MS medium. TDZ, zeatin, and BA treatments were not effective on the induction of multiple shoot in vitro culture. As a result, in vitro culture of new Saxifraga fortunei, 'Greenstar' with $2.0mg{\cdot}L^{-1}$ of CPPU in 1/2 MS medium was most effective for the rapid multiplication.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.111-111
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• 2022

According to data from the Ministry of Environment, food waste accounted for 27% of the nation5 s household waste in 2020, and 4.67 million tons of food waste is being discharged per year. According to the Food Waste Direct Landfill Prohibition Act, food waste must be incinerated, composted, fodder, and decomposed before landfilling. The cost of incineration and landfilling of food waste is considerable. Therefore, through the process of turning food waste into fertilizer, we are going to investigate the limit of crop application and the change in the growth of crops during continuous use of food waste fertilizer. This study investigated the growth of lettuce such as shoot length, root length, leaf number, fresh weight, and dry weight after treating lettuce with food waste dry powder mixed fertilizer. The experiment was carried out continuously in 2021 (1st year) and 2022 (2nd year), and the treatment groups were set to No Treatment (NT), Chemical Fertilizer (CF), Mixed Fertilizer (MF×1), and Mixed Fertilizer×2 (MF×2), was repeated 3 times. As a result of the 1st year growth survey, there was no significant difference between NT and CF in the case of shoot length, but MF×1 and MF×2 were significantly decreased compared to NT. Root length was not significantly different in all treatment groups. As for the leaf number, there was no significant difference between NT and MF×1, but CF and MF×2 were significantly decreased compared to NT. In fresh weight, MF×1 and MF×2 were significantly decreased compared to NT, and in the case of dry weight, there was no significant difference between NT, MF×1, and MF×2. As a result of the 2nd year growth survey, there was a significant difference in CF and MF×2 in leaf number, but there was no significant difference in all treatment groups with respect to shoot length, root length, fresh weight, and dry weight. Through continuous additional research, it is necessary to confirm the change in soil composition and the growth of crops due to food waste fertilizer treatment.

• Horticultural Science & Technology
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• v.32 no.4
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• pp.421-426
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• 2014

To investigate the optimal size of fruit bearing branches (FBBs) of 'Kawanakajima Hakuto' peach (Prunus persica Batsch) for production of high quality fruits, we evaluated shoot growth and fruit characteristics relative to FBB direction and thickness. FBB diameter, shoot length, fruit weight, and soluble solids content (SSC) averaged 6.9 mm, 32.6 cm, 333.6 g, and $11.2^{\circ}Brix$, respectively. Coefficients of variation of FBB diameter and shoot length were high as 35.8% and 75.3%. The highest frequency values revealed that 76.4% of FBBs had a diameter below 9.0 mm, 70.0% of shoot lengths were less than 40 cm, 24.8% of fruits were 310-340 g, and 66.9% of SSCs were $10.5-12.5^{\circ}Brix$. Shoot length and SSC were significantly affected by FBB diameter without direction, and fruit weight was significantly affected by interaction of FBB diameter and direction. There was a negative correlation ($r^2=0.094^*$) of FBB diameter and fruit weight with upward FBB directions, whereas FBB diameter and fruit weight were positively correlated ($r^2=0.246^{**}$) with downward FBB direction. With thick FBB diameter, shoot length, and SSC were significantly increased, but fruit weight was decreased. Therefore, downward FBBs below 9.0 mm in thickness are suitable for producing high-quality fruits of 'Kawanakajima Hakuto' peach.

• Journal of Korean Society of Forest Science
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• v.79 no.4
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• pp.359-366
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• 1990

This study was carried out to investigate rooting rate, plagiotrophic growth and root-promoting effect of IBA in Taxus cuspidata S, et Z. cuttings. The results obtained were summarized as follows ; 1. As the ortet ages of cuttings were increased in 5, 10, to 20 years, the rooting rates were decreased in order of 85.7%, 81.7% and 62.4%, and the number of primary roots were also decreased in 5.3, 3.7 and 2.9, respectively. 2. Rooting rate by cutting types of lateral shoot were higher than those of main shoot type, significant at the 1% level. 3. IBA(l00ppm) treatment promoted the rooting rates and the number of primary roots. 4. As the ortet ages were increased in 5, 10 to 20 years, angles of central shoot of rooted cuttings which mesured cyclophysis were decreased in order of $75.9^{\circ}$, $68.5^{\circ}$ and $59.6^{\circ}$, respectively, significant at the 1% level. 5. Average angles showed $77.0^{\circ}$ for main shoot cutting, $65.9^{\circ}$ for upper lateral shoot and $61.7^{\circ}$ for lower lateral, which was significant at 1% level, and lateral shoot cutting showed more topophysis than main shoot cutting. 6. Shoot length of rooted cuttings from main shoot cutting was longer than that from lateral shoot significant at the 1% level. 7. Relationship between some characteristics showed highly positive correlation.