• The Korea Journal of Herbology
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• v.23 no.3
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• pp.127-134
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• 2008

Objectives : The present study was designed to investigate whether Ostericum koreanum (OK) could regulate lipopolysaccharide (LPS)-induced inflammatory response in vitro and in vivo. Methods : To evaluate of anti-inflammatory effect of OK, we examined Nitric oxide (NO), proinflammatory cytokines production in LPS-stimulated RAW264.7 cells. Furthermore, we checked molecular mechanism especially in the phosphorylation of mitogen-activated protein kinases (MAPKs) and the degradation of inhibitory kappa B a ($Ik-B{\alpha}$) using western blot and also investigated survival of mice in LPS-mediated endotoxin shock. Results : 1. Extract from OK itself have weak cytotoxic effect on RAW264.7 cells. Extract from OK inhibited LPS-induced NO, tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), interleukin $(IL)-1{\beta}$, IL-6 and IL-10 production in RAW264.7 cells. 2. OK inhibited the phosphorylation of MAPKs, such as p38, extracelluar signal-regulated kinase (ERK1/2) and c-Jun NH2-terminal kinase (JNK) and also the degradation of $I{\kappa}-B{\alpha}$ in the LPS-stimulated RAW264.7 cells 3. OK did not inhibit LPS-induced endotoxin shock. Conclusions : OK down-regulated LPS-induced NO and cytokines production through suppressing activation of MAPKs and degradation of $I{\kappa}-B{\alpha}$. Our results suggested that OK may be a beneficial drug against inflammatory diseases.

• Korean Journal of Clinical Pharmacy
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• v.27 no.4
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• pp.221-227
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• 2017

Background: As personalized healthcare industry has attracted much attention, big data analysis of healthcare data is essential. Lots of healthcare data such as product labeling, biomedical literature and social media data are unstructured, extracting meaningful information from the unstructured text data are becoming important. In particular, text mining for adverse drug reactions (ADRs) reports is able to provide signal information to predict and detect adverse drug reactions. There has been no study on text analysis of expert opinion on Korea Adverse Event Reporting System (KAERS) databases in Korea. Methods: Expert opinion text of KAERS database provided by Korea Institute of Drug Safety & Risk Management (KIDS-KD) are analyzed. To understand the whole text, word frequency analysis are performed, and to look for important keywords from the text TF-IDF weight analysis are performed. Also, related keywords with the important keywords are presented by calculating correlation coefficient. Results: Among total 90,522 reports, 120 insulin ADR report and 858 tramadol ADR report were analyzed. The ADRs such as dizziness, headache, vomiting, dyspepsia, and shock were ranked in order in the insulin data, while the ADR symptoms such as vomiting, 어지러움, dizziness, dyspepsia and constipation were ranked in order in the tramadol data as the most frequently used keywords. Conclusion: Using text mining of the expert opinion in KIDS-KD, frequently mentioned ADRs and medications are easily recovered. Text mining in ADRs research is able to play an important role in detecting signal information and prediction of ADRs.

• Molecules and Cells
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• v.21 no.3
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• pp.381-388
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• 2006

Heat shock proteins (Hsp) 70 are a ubiquitous family of molecular chaperones involved in many cellular processes. A yeast strain, ssa1/2, with two functionally redundant cytosolic Hsp70s (SSA1 and SSA2) deleted shows thermotolerance comparable to mildly heatshocked wild type yeast, as well as increased protein synthesis and ubiquitin-proteasome protein degradation. Since mRNA abundance does not always correlate well with protein expression levels it is essential to study proteins directly. We used a gel-based approach to identify stress-responsive proteins in the ssa1/2 mutant and identified 43 differentially expressed spots. These were trypsin-digested and analyzed by nano electrospray ionization liquid chromatography tandem mass spectrometry (nESI-LC-MS/MS). A total of 22 non-redundant proteins were identified, 11 of which were confirmed by N-terminal sequencing. Nine proteins, most of which were up-regulated (2-fold or more) in the ssa1/2 mutant, proved to be stress-inducible proteins such as molecular chaperones and anti-oxidant proteins, or proteins related to carbohydrate metabolism. Interestingly, a translational factor Hyp2p up-regulated in the mutant was also found to be highly phosphorylated. These results indicate that the cytosolic Hsp70s, Ssa1p and Ssa2p, regulate an abundance of proteins mainly involved in stress responses and protein synthesis.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.2
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• pp.385-389
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• 2008

Nitric oxide (NO) has been suggested to play an important role in endotoxin-mediated shock and inflammation. In this study, we investigated the effect of Rosa laevigata Michx. (Rosaceae) on the production of NO and the molecular mechanism of its action. Rosa laevigata inhibited NO generation and iNOS expression in LPS-stimulated murine macrophages. Activity of nuclear $factor{-\kappa}B\;(NF{-\kappa}B)$ and the degradation of $I{\kappa}B-{\alpha}$ were suppressed by Rosa laevigata. Furthermore, extracellular signal-stimulated kinase (ERK), which is known to be involved in $NF{-\kappa}B$ activation, is inhibited by Rosa laevigata. These results suggest that Rosa laevigata could exert its anti-inflammatory actions by suppressing the synthesis of NO through inhibition of $NF{-\kappa}B$ activity.

• The Journal of the Korea institute of electronic communication sciences
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• v.11 no.6
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• pp.605-610
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• 2016

The ELCB(: Earth Leakage Circuit Breaker) is the only way being used to prevent accidents from happening caused by electrical disaster. However, the existing ELCB has a limit to prevent damages to life and property due to a electric fire and a human body electric shock caused by the resistive leakage current, because of detecting the total leakage current in the block range of 15mA~30mA. It also has problems such as reduced productivity and reliability due to malfunctions by capacitive leakage currents. In this study, we have implemented the algorithm for the resistive leakage current detection technique and developed the resistive leakage current detection module based on a MSP430 processor, 16bit signal processor and this module can be operated in a desired block threshold within 0.03 seconds as specified in KS C 4613.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.13 no.1
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• pp.1-14
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• 2014

The expansion of the physical road in response to changes in social conditions and policy of the country has reached the limit. In order to alleviate congestion on the existing road to reconsider the effectiveness of this method should be asking. Currently, how to collect traffic information for management of the intersection is limited to point detection systems. Intelligent Transport Systems (ITS) was the traffic information collection system of point detection method such as through video and loop detector in the past. However, intelligent transportation systems of the next generation(C-ITS) has evolved rapidly in real time interval detection system of collecting various systems between the pedestrian, road, and car. Therefore, this study is designed to evaluate the development of an algorithm for queue length based real-time traffic signal control methodology. Four coordinates estimate on time-space diagram using the travel time each individual vehicle collected via the interval detector. Using the coordinate value estimated during the cycle for estimating the velocity of the shock wave the queue is created. Using the queue length is estimated, and determine the signal timing the total queue length is minimized at intersection. Therefore, in this study, it was confirmed that the calculation of the signal timing of the intersection queue is minimized.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.35 no.1
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• pp.19-24
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• 1999

This paper is second part on the auditory thresholds and fish behaviors to the underwater sounds for luring of target species at the set-net in the coast of Cheju. In order to obtain the critical ratio of yellow tails(Seriola quinqueradiata) and the emission level of underwater sound for luring of them, we make experiments to measure the auditory threshold of them using conditioning with electric shock. In state that the white noise with 10dB higher sound pressure level than ambient noise is emitted, the auditory thresholds of yellow tails are measured with 100~116.5dB and they are higher than those in state of no emission of white noise by the masking effects of it. Although sound pressure level of background noise go down, the auditory thresholds go up with frequency above than 300Hz.The critical ratio of yellow-tails in frequency of 80Hz, 100Hz, 200Hz, 500Hz, 800Hz are 46dB, 40dB, 50dB, 52dB, 60dB, 70dB respectively. The sound pressure level of which the signal sound is recognized by yellow tails under the ambient noise is above 100dB and the critical ratio of them is above 40dB.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.5 no.7
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• pp.1210-1218
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• 2001

In this paper, A X-band voltage-controlled hair-pin resonator oscillator(VCHRO) is able to a local oscillator or a signal source in transmitter/receiver of a microwave communication system for mobile radar, is designed and fabricated In order to apply mobile radar system is used the hair-pin resonator stronger on shock or vibration than the dielectric resonator, and also, in order to improvement the phase noise and output power is used a system of serial feedback format A hair-pin resonator was simulated by momentum method of HP ADS and then a oscillator circuit was designed that operates at 10.525 GHz by nonlinear method in harmonic balance simulation. The HRO generated output power of 6.93 dBm at 10.525 GHz, phase noise of -57.74 dBc at 100 kHz offset from carrier and the 2'nd harmonic was suppressed -23.90 dBc.

• The Korean Journal of Physiology and Pharmacology
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• v.19 no.6
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• pp.515-522
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• 2015

Notch signaling is a key regulator of neuronal fate during embryonic development, but its function in the adult brain is still largely unknown. Mind bomb-2 (Mib2) is an essential positive regulator of the Notch pathway, which acts in the Notch signal-sending cells. Therefore, genetic deletion of Mib2 in the mouse brain might help understand Notch signaling-mediated cell-cell interactions between neurons and their physiological function. Here we show that deletion of Mib2 in the mouse brain results in impaired hippocampal spatial memory and contextual fear memory. Accordingly, we found impaired hippocampal synaptic plasticity in Mib2 knock-out (KO) mice; however, basal synaptic transmission did not change at the Schaffer collateral-CA1 synapses. Using western blot analysis, we found that the level of cleaved Notch1 was lower in Mib2 KO mice than in wild type (WT) littermates after mild foot shock. Taken together, these data suggest that Mib2 plays a critical role in synaptic plasticity and spatial memory through the Notch signaling pathway.

• The Journal of the Acoustical Society of Korea
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• v.25 no.1E
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• pp.32-35
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• 2006

The screech tone of underexpanded jet is numerically calculated without any specific modeling for the screech tone itself. Fourth-order optimized compact scheme and fourth-order Runge-Kutta method are used to solve the 2D axisymmetric Euler equation. Adaptive nonlinear artificial dissipation model and generalized characteristic boundary condition are also used. The screech tone, generated by a closed loop between instability waves and quasi-periodic shock cells at the near field, is reasonably analyzed with present numerical methods for the underexpanded jet having Mach number 1.13. First of all, the centerline mean pressure distribution is calculated and compared with experimental and other numerical results. The instantaneous density contour plot shows Mach waves due to mixing layer convecting supersonically, which propagate downstream. The pressure signal and its Fourier transform at upstream and downstream shows the directivity pattern of screech tone very clearly. Most of all, we can simulate the axisymmetric mode change of screech tone very precisely with present method. It can be concluded that the basic phenomenon of screech tone including the frequency can be calculated by using high-order and high-resolution schemes without any specific numerical modeling for screech tone feedback loop.