Inhibitory Effect of Extract from Ostericum koreanum on LPS-induced Proinflammatory Cytokines Production in RAW264.7 Cells

LPS로 자극한 RAW264.7 세포에서 강활 추출물의 염증성세포활성물질의 억제효과

  • Park, Hee-Je (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Bae, Gi-Sang (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Do-Yun (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Seo, Sang-Wan (Dept. of Microbiology, College of Medicine, Kyung Hee University) ;
  • Park, Kyung-Bae (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Kim, Byung-Jin (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Song, Je-Moon (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Kyung-Yong (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Na, Chul (Dept. of Oriental Rehabilitation Medicine, IKsan Wonkwang Oriental Hospital) ;
  • Shin, Byung-Chul (Dept. of Oriental Rehabilitation Medicine, IKsan Wonkwang Oriental Hospital) ;
  • Park, Sung-Joo (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Song, Ho-Joon (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Hwang, Sung-Yeon (Dept. of Herbology, College of Oriental Medicine, Wonkwang University)
  • 박희제 (원광대학교 한의과대학 본초학교실) ;
  • 배기상 (원광대학교 한의과대학 본초학교실) ;
  • 김도윤 (원광대학교 약학대학 한약학과) ;
  • 서상완 (경희대학교 의과대학 미생물학교실) ;
  • 박경배 (원광대학교 약학대학 한약학과) ;
  • 김병진 (원광대학교 약학대학 한약학과) ;
  • 송제문 (원광대학교 한의과대학 본초학교실) ;
  • 이경용 (원광대학교 한의과대학 본초학교실) ;
  • 나철 (원광대학교 한방병원 재활의학과) ;
  • 신병철 (원광대학교 한방병원 재활의학과) ;
  • 박성주 (원광대학교 한의과대학 본초학교실) ;
  • 송호준 (원광대학교 한의과대학 본초학교실) ;
  • 황성연 (원광대학교 한의과대학 본초학교실)
  • Published : 2008.09.30

Abstract

Objectives : The present study was designed to investigate whether Ostericum koreanum (OK) could regulate lipopolysaccharide (LPS)-induced inflammatory response in vitro and in vivo. Methods : To evaluate of anti-inflammatory effect of OK, we examined Nitric oxide (NO), proinflammatory cytokines production in LPS-stimulated RAW264.7 cells. Furthermore, we checked molecular mechanism especially in the phosphorylation of mitogen-activated protein kinases (MAPKs) and the degradation of inhibitory kappa B a ($Ik-B{\alpha}$) using western blot and also investigated survival of mice in LPS-mediated endotoxin shock. Results : 1. Extract from OK itself have weak cytotoxic effect on RAW264.7 cells. Extract from OK inhibited LPS-induced NO, tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), interleukin $(IL)-1{\beta}$, IL-6 and IL-10 production in RAW264.7 cells. 2. OK inhibited the phosphorylation of MAPKs, such as p38, extracelluar signal-regulated kinase (ERK1/2) and c-Jun NH2-terminal kinase (JNK) and also the degradation of $I{\kappa}-B{\alpha}$ in the LPS-stimulated RAW264.7 cells 3. OK did not inhibit LPS-induced endotoxin shock. Conclusions : OK down-regulated LPS-induced NO and cytokines production through suppressing activation of MAPKs and degradation of $I{\kappa}-B{\alpha}$. Our results suggested that OK may be a beneficial drug against inflammatory diseases.

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