• 제목/요약/키워드: Shiranuhi

검색결과 27건 처리시간 0.031초

부지화 감귤에서 자근의 발생 (Outbreak of Scion Root from 'Shiranuhi Mandarin' Hybrid Tree in Plastic Film House)

  • 강석범;문영일;이동훈;김용호;한승갑;채치원
    • 한국환경농학회지
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    • 제31권4호
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    • pp.313-317
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    • 2012
  • 감귤은 접목을 통해 일반적으로 번식을 한다. 국내에서 온주밀감의 주요 대목으로 이용되는 탱자(Poncirus trifoliata)는 다른 대목에 비해 왜성이고 내한성이 강한 특징을 갖고 있다. 부지화((Citrus unshiu ${\times}$ C. sinensis) C. reticulata) 감귤에서도 이러한 탱자대목의 우수한 특성 때문에 주요 대목으로 탱자를 이용하고 있고 이 대목에서 발생된 뿌리는 당연히 탱자뿌리로 인식하고 있다. 그러나 몇 년 전부터 부지화 감귤나무에서 착화량 감소가 농가들 사이에서 문제시 되어왔는데 이 원인에 대해서는 강전정, 과다착과, 생육기간의 온도에 의해 발생되었다고 추측하였다. 이러한 착화량 감소가 부지화의 자근에 의해 발생이 되었다고 생각하는 연구자는 거의 없었다. 그래서 본 연구는 국내에서 부지화 감귤 나무에서 자근의 존재여부를 밝히기 위하여 수행되었다. 본 연구를 위해 부지화 재배 농가들 중 착화가 불량한 과원당 나무 3~4주를 무작위로 선정하여 조사를 수행하였다. 뿌리는 각 조사나무에서 표토 5cm 부위에서 잔뿌리를 채취하여 DNA를 추출한 뒤 품종 판별에 이용하였다. 부지화 자근 판별에는 F2/R15, F4/R15, F5/R15의 3가지 프라이머 세트를 이용하였다. DNA 분석결과, 착화가 불량한 부지화 20주 중 14주가 자근이 발생된 것으로, 나머지 6주는 다른 요인에 의해 착화가 불량한 것으로 밝혀졌는데 이러한 결과는 국내에서 처음 보고되는 결과이다. 부지화 자근이 발생된 뿌리는 탱자보다 표면이 어두웠으며 직경도 더 컸다. 자근발생여부를 확인하기 위해 F2/R15, F4/R15, F5/R15 프라이머 세트를 이용하였을 때 부지화 자근과 탱자뿌리 판별이 가능하였고 자근이 실제 재배현장에서 발생되고 있음을 알 수 있었다. 자근이 발생된 나무는 정상대목의 나무에 비해 착과량이 유의하게 감소하였다. 금번 결과를 통해서 봤을 때 부지화 재배과원에서 자근의 발생과 그 영향에 대한 조사와 연구가 시급히 필요할 것으로 생각되며 그에 따른 재배법 개발도 뒤따라야 할 것으로 판단되었다.

Effect of Scion Root Occurrence on the Flowering, Fruit Quality and Yield of 'Shiranuhi' Mandarin Hybrid in Plastic Film House

  • Kang, Seok-Beom;Moon, Young-Eel;Kim, Yong-Ho
    • 한국토양비료학회지
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    • 제46권6호
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    • pp.525-529
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    • 2013
  • As most of citrus, shiranuhi mandarin ((Citrus unshiu ${\times}$ C. sinensis) ${\times}$ C. reticulata) mainly use the trifoliate orange (Poncirus trifoliata) as its rootstock which has dwarf and cold hardness in Korea. However, recently, scion root was observed in 'Shiranuhi' mandarin tree grafted onto the trifoliate orange. This study was carried out to find out effects of scion root occurrence on the fruit quality, flowering and yield of shiranuhi mandarin. For the experiment, we selected six farmers who have outbreak of scion root in their Shiranuhi mandarin orchards and surveyed the difference of fruit quality of shiranuhi mandarin hybrid between scion root and control (trifoliate orange root). In the results, flowering was severely decreased in scion root trees compared to control. As a result of survey of 174 Shiranuhi mandarins, 160 trees were proved to be scion root, and had less flowering. Fruiting of scion root also severely dropped compared to control and thus, yields of scion root (6.4 kg) reduced by 24% compared to control (26.7 kg). The fruit size, weight and soluble solid contents of scion root were significantly reduced, but there were no differences in acid contents and coloring of fruit. From the results, we concluded that scion root had negative influence on flowering, fruit size and the yields of Shrinanuhi mandarin hybrid.

Evaluation of Shiranuhi, a Hybrid of Kiyomi Tangor and Nakano No.3 Ponkan, for Resistance to Citrus Canker in Growth Chamber

  • Myung, Inn-Shik;Hyun, Jae-Wook;Kim, Kwang-Sik;Lee, Seong-Chan;Lim, Han-Cheol
    • The Plant Pathology Journal
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    • 제19권5호
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    • pp.253-256
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    • 2003
  • Citrus canker disease is caused by bacteria Xanthomonas axonopodis .pv. Citri. Shiranuhi cultivar, a hybrid of Kiyomi tangor and Nakano No.3 ponkan was evaluated for resistance to citrus canker based on initiation of disease, percent area of lesion infected and growth rate of bacteria in the leaf under growth chamber condition. Significant differences between susceptible plant and resistant plants were observed in these assays. Resistant plants showed delayed disease symptoms compared to the susceptible plants after spray inoculation of the pathogen. The resistant verities, satsuma, yuzu, and Shiranuhi showed symptoms after six days where as susceptible, mexican lime showed the symptoms just after three days of inoculation. 18 days after inoculation, percent area of lesions developed on leaf and disease severity differed significantly in susceptible and resistant plants, and were ranked as follows: mexican lime > early satsuma =Shiranuhi =yuzu (P <, 0.01). However, 30 days after inoculation, percent area of lesion was further differentiated into resistant and highly resistant plants. That was ranked as follows: sweet orange> early satsuma =Shiranuhi =Kiyomi > yuzu (P < 0.01). These results indicate that host reaction to the bacterial was more distinct when the disease developed for a longer period. Growth rates of a citrus canker bacterium during 16 40 h also were distinct after infiltration into leaves of susceptible and resistant plants, and were ranked as follows: sweet orange> early satsuma =Shiranuhi =Kiyomi =yuzu (P < 0.01). Based on these results, we concluded that Shiranuhi is resistant to citrus canker as compared to Kiyomi, early satsuma, and yuzu.

Anti-inflammatory Polymethoxyflavones Isolated from the Branches of Shiranuhi Tree

  • Jo, Yeon Jeong;Lee, Nam Ho
    • 대한화학회지
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    • 제65권5호
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    • pp.327-332
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    • 2021
  • Shiranuhi is a fruit of Citrus species widely cultivated in Jeju Island, Korea. From an extract of Shiranuhi tree branches were identified five polymethoxyflavones possessing anti-inflammatory effects; nobiletin (1), sinensetin (2), tetramethylscutellarein (3), 6-hydroxy-5,7,3',4'-tetramethoxyflavone (4) and 5-desmethylsinensetin (5). Evaluation of the activities was conducted by monitoring the production of nitric oxide (NO), prostaglandin E2 and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) as well as the levels of iNOS and COX-2 protein expression in LPS-induced RAW264.7 cells. Among the isolates, the compound 4 exhibited the most significant NO inhibition, and suppressed the levels of iNOS and related cytokines. Therefore, it was suggested that the extract and constituents from Shiranuhi tree branches could be useful as anti-inflammatory ingredient.

최근 부지화 감귤 품종에 발생하는 식물병의 종류 및 그 증상 (Diseases and the Symptoms Recently Occurred on 'Shiranuhi' Citrus Cultivar in Jeju Island)

  • 현재욱;김동환;김광식;이성찬;고상욱;임한철
    • 식물병연구
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    • 제10권2호
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    • pp.94-99
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    • 2004
  • 청견과 퐁깐의 교배종인 부지화 감귤은 그 모양이 독특하고 당 함량이 높은 고품질의 감귤로서 최근 재배면적과 농가수가 크게 증가하고 있는 품종이다. 대부분이 비닐하우스에서 재배되기 때문에 병이 크게 문제가 되지 않았지만 최근 몇몇 농가에서 병이 발생하여 큰 피해를 주는 경우가 있었다. 조사 결과 부지화 하우스에서 주로 발생하는 병은 Phytophthora citrophthora에 의한 역병, Alternaria sp.에 의한 배꼽썩음병, Penicillium digitatum에 의한 녹색곰팡이병, Botrytis cinerea에 의한 잿빛곰팡이병, Diaporthe citri에 의한 꼭지썩음병 그리고 Xanthomonas axonopodis pv. citri에 의한 궤양병 등 6종이 조사되었다.

Effect of Harvesting Season on the 6-Cryptoxanthin in Shiranuhi Mandarin Fruit Cultivated in Jeju Island

  • Heo, Ji-Man;Kim, Do-Hyun;Kim, In-Jung;Lee, Sam-Pin;Kim, Chan-Shick
    • Preventive Nutrition and Food Science
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    • 제10권3호
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    • pp.219-223
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    • 2005
  • [ $\beta-Cryptoxanthin$ ] content was determined in Shiranuhi mandarin fruits harvested at monthly intervals from October to February in Jeju Island. Crude carotenoids were extracted from both peel and flesh of Shiranuhi mandarin fruits and analyzed using TLC and HPLC; $\beta-cryptoxanthin$ was indicated the Rr value of 3.2 and retention time of 23 min, respectively. $\beta-Cryptoxanthin$ contents in both peel and flesh were increased gradually as the citrus fruits ripened fully until harvesting season (February). According to the harvesting time, $\beta-cryptoxanthin$ contents in the peel were $0.15\;mg\%\;(October),\;0.28\;mg\%\;(November),\;0.38\;mg\%\;(December),\;1.23\;mg\%\;(January),\;and\;1.71\;mg\%\;(February).$In the flesh, $\beta-cryptoxanthin$ contents were lower than those of peels, having $0.06\;mg\%\;(October),\;0.08\;mg\%\;(November),\;0.19\;mg\%\;(December),\;0.26\;mg\%\;(January),\;and\;0.65\;mg\%\;(February).$ These results demonstrate that $\beta-cryptoxanthin$ in Shiranuhi mandarin fruits accumulated during ripening of the citrus fruits. In particular, the peels had much higher concentrations of $\beta-cryptoxanthin$ and have potential for use as a functional ingredient.

Evaluation of Potential of Mandarin Hybrid 'Shiranuhi' against inoculation of Bacterial Canker Disease Pathogen (Xanthomonas axonopodis pv. citri) in Citrus Field in Jeju Island

  • Hyun, Jae-Wook;Myung, Inn-Shik;Lee, Seong-Chan;Kim, Kwang-Sik;Lim, Han-Cheol
    • The Plant Pathology Journal
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    • 제19권5호
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    • pp.248-252
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    • 2003
  • This study was carried out to evaluate potential of Shiranuhi cultivar against inoculation of causal pathogen of citrus canker, Xanthomonas axonopodis pv. citri in Jeju Island by comparing degree of susceptibility of fruits and leaves/twigs, and analysis of incidence of canker disease. Progression of symptom, disease incidence, and percent area of lesion were surveyed for evaluation of resistance. In greenhouse condition, symptoms of bacterial citrus canker progressed more rapidly in sweet orange, a moderately susceptible cultivar, than in other four cultivars (satsuma mandarin, 'Kiyomi', 'Shiranuhi' and 'Yuzu'). At 20 days after inoculation, disease severity was the highest in sweet orange (5.0$\pm$0.0), and all tested leaves were distorted or had dropped. On the other hand, 'Yuzu' showed the lowest disease severity (2.6$\pm$0.47), followed by 'Kiyomi' (4.0$\pm$0.0), 'Shiranuhi' (4.0$\pm$0.82), and satsuma mandarin (4.3$\pm$0.47). Percent area of lesion per leaf 30 days after inoculation was the highest in sweet orange (8.31$\pm$1.78), followed by satsuma mandarin (1.51$\pm$1.25), 'Shiranuhi' (1.39$\pm$0.94), and 'Kiyomi' (1.1$\pm$0.9), while the lowest was in 'Yuzu' (0.26$\pm$0.17). Infield condition, percentage of diseased leaf in 'Shiranuhi' was very low, 5.2$\pm$2.9, compared with sweet orange, 71.0$\pm$ 11.5, while that of satsuma mandarin and 'Kiyomi' were 6.9$\pm$7.0 and 4.3$\pm$2.0, respectively. Percentages of diseased leaf was higher (17.4$\pm$7.1) than that of diseased fruit (3.2$\pm$2.5) in severely diseased trees of Shiranuhi cultivar, and the disease was not observed on twig in open field condition. Lesion sizes on leaves and fruits in open field condition were 4.1$\pm$2.2 mm2 and 5.1$\pm$5.6 mm2, respectively, while those in greenhouse condition were 8.7$\pm$5.7 mm2, 10.4$\pm$9.2 mm2 and 5.6$\pm$2.6 mm2 on leaves, fruits and twigs, respectively. The disease was observed in 5.6% out of total 107 farmers Shiranuhi fields under polyethylene film house, and average percentages of diseased tree in 31 fields of Shiranuhi cultivar and adjacent satsuma mandarin fields were 0.02% and 14.8%, respectively. Average percentage of diseased fruit was 1.6% in satsuma mandarin which was not observed in anyone of all the 31 Shiranuhi farmers fields. Therefore, it was concluded that 'Shiranuhi' cultivar is not potential against causal pathogen of citrus canker disease in Jeju Island because the cultivar has similar resistance as satsuma mandarin which occupies over 95% of total 25,000 ha in Jeju Island in polyethylene film houses protected from outside.

부지화 자근발생 감귤나무의 착화와 수량에 미치는 환상박피의 영향 (Effect of Girdling on the Flowering and Yield in Scion Rooted 'Shiranuhi' Mandarin grown in Plastic Film House)

  • 강석범;문영일;한승갑;이혜진;최영훈
    • 한국환경농학회지
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    • 제35권4호
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    • pp.256-262
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    • 2016
  • BACKGROUND: 'Shiranuhi' mandarin is one of the popular citrus cultivars in Jeju Island, Korea. However, the emergence of scion roots since the past few years has altered its flowering, fruiting, and quality. Girdling of branches is one of the methods of increasing flowering in citrus trees. METHODS AND RESULTS: This experiment was conducted to determine the effect of girdling on the flowering and yields of scion rooted 'Shiranuhi' mandarin hybrid. We selected normal trees without scion roots as controls. The trees with scion roots were divided into two groups: trees without girdling and with girdling on main branches. Each group contained five replications and the experiment was conducted in Gosan and Harye of Jeju Island. The scion rooted trees revealed severely decreased flowering and low flowering/leaf ratios; however, the leaf/fruit ratio significantly increased. But, girdling on main branches significantly increased flowering and the flowering/leaf ratio. In the scion rooted trees, yields dropped due to poor flowering; however, girdling of branches efficiently improved the yields of the trees. Fruit quality, fruit size, and fruit weight of scion rooted trees were low in comparison with the control, whereas girdling of the branches improved flowering and the fruit weight to some extent. No significant difference in soluble solid contents was observed. CONCLUSION: Girdling is an effective method to induce flowering of the scion rooted 'Shiranuhi' mandarin trees. In addition, yields of scion rooted trees were improved.

부지화 미숙과 에센셜 오일의 항산화 및 항균 활성 효과 (Influence of Essential Oil in 'Shiranuhi' Immature Fruit on Antioxidant and Antimicrobial Activities)

  • 김상숙;현주미;김광식;박경진;박석만;최영훈
    • 한국약용작물학회지
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    • 제21권6호
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    • pp.493-497
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    • 2013
  • This study was designed to analyze the chemical composition of essential oil in 'Shiranuhi' immature fruit and to test their biological activities. 'Shiranuhi' immature essential oils (SIEO) were obtained by steam distillation from fruits collected from Jeju Island and were analyzed using gas chromatograph (GC)-flame ionization detectors (FID) and GC-MS. Fourteen components were identified in the essential oil. Limonene (75.21%) and terpineol (8.68%) were the major components in SIEO. Since acne vulgaris is the combined result of a bacterial infection and the inflammatory response to that infection, we examined whether SIEO possessed antibacterial against skin pathogens. As a result, SIEO showed excellent antibacterial activities against drug-susceptible and -resistant Propionibacterium acnes and Staphylococcus epidermidis, which are acne-causing bacteria. In this study, SIEO was examined on DPPH radical scavenging activities, which showed moderate antioxidant activity ($SC_{50}$, $15.36{\mu}L/mL$). In order to determined whether SIEO can be safely applied to human skin, the cytotoxicity effects of SIEO were determined by colorimetric MTT assays in normal human fibroblasts and keratinocyte HaCaT cells. They exhibited low cytotoxicity at $0.5{\mu}L/mL$ in both celllines. Based on these results, we suggest the possibility that essential oil of 'Shiranuhi' maybe considered as an antibacterial and antioxidant agent.

자근발생이 부지화 감귤나무의 수체 생육과 뿌리내 양분함량에 미치는 영향 (Effect of Occurrence of Scion Root on the Growth and Root Nutrient Contents of 'Shiranuhi' Mandarin Hybrid grown in Plastic Film House)

  • 강석범;문영일;양경록;좌재호;한승갑;이혜진;박우정
    • 한국환경농학회지
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    • 제38권3호
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    • pp.154-158
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    • 2019
  • BACKGROUND: 'Shiranuhi' mandarin is a major cultivar among all late ripening type of citrus, and is widely cultivated in Korea. However, many farmers have reported scion root problems in their orchard resulting in reduced flowering and fruiting. It is necessary that the physiology of scion-rooted 'Shiranuhi' mandarin trees is further understood. METHODS AND RESULTS: This experiment was conducted to understand the growth response and physiology of scion-rooted 'Shiranuhi' mandarin hybrids. In our study, 'Shiranuhi' mandarin trees were divided into two groups: trees without scion roots (control) and trees with scion roots. The experiment was conducted in Seogwipo of Jeju, with ten replicates for each group. Growth of trees with scion roots was more vigorous and the trees were taller than the controls. Tree height and trunk diameter of scion-rooted trees were significantly higher than those of control trees. Exposed length of rootstocks of scion-rooted trees was significantly lower (by about 2 cm) than that of control trees (8.6 cm). In terms of root nutrition, carbon contents of scion-rooted trees was significantly lower than that of control trees, but nitrogen and potassium concentrations in scion roots were significantly higher than those in control roots. CONCLUSION: Based on the results, we infer that growth of scion-rooted trees was very vigorous and the content of nitrogen in these roots was higher than that in the control tree roots. Thus, the carbon/nitrogen ratio of scion roots was significantly lower than that of the control roots.