• Title/Summary/Keyword: Sheet Model

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3D Light-Sheet Fluorescence Microscopy of Cranial Neurons and Vasculature during Zebrafish Embryogenesis

  • Park, Ok Kyu;Kwak, Jina;Jung, Yoo Jung;Kim, Young Ho;Hong, Hyun-Seok;Hwang, Byung Joon;Kwon, Seung-Hae;Kee, Yun
    • Molecules and Cells
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    • v.38 no.11
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    • pp.975-981
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    • 2015
  • Precise 3D spatial mapping of cells and their connections within living tissues is required to fully understand developmental processes and neural activities. Zebrafish embryos are relatively small and optically transparent, making them the vertebrate model of choice for live in vivo imaging. However, embryonic brains cannot be imaged in their entirety by confocal or two-photon microscopy due to limitations in optical range and scanning speed. Here, we use light-sheet fluorescence microscopy to overcome these limitations and image the entire head of live transgenic zebrafish embryos. We simultaneously imaged cranial neurons and blood vessels during embryogenesis, generating comprehensive 3D maps that provide insight into the coordinated morphogenesis of the nervous system and vasculature during early development. In addition, blood cells circulating through the entire head, vagal and cardiac vasculature were also visualized at high resolution in a 3D movie. These data provide the foundation for the construction of a complete 4D atlas of zebrafish embryogenesis and neural activity.

Non-Contact Pick-up System for Turning Large Flexible Thin Sheets (대형 유연박판 회전이송용 비접촉 파지시스템 설계)

  • Kim, Joon Hyun;Ahn, Sung Wook;Lee, Se Jin
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.23 no.5
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    • pp.435-442
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    • 2014
  • This paper describes an improved design model that can be used to configure a non-contact pneumatic device to turn a large sheet at the in-line system. For rotational moving in the conveyor system, the conventional method is to turn the system itself. The improved non-contact pick-up system mainly uses 8 pairs of L-shaped latches and 12 swirl type heads. It is positioned above the upward air flow table. This system performs the non-contact gripping and side-edge contact support in the vertical and rotational directions to hold the self-weight of a large flat sheet. A non-contact air head can exert a sufficient gripping ability at 4N lower than the standard working pressure. The side latches support 60% of the lifting force required. Through structural and flow analysis, the working conditions were simultaneously considered in accordance with the deflection and flatness of the glass.

Studies on the suppression of transmission of anthracnose with covering method and environment friendly agricultural materials (EFAM) in pepper field

  • Kang, B.R.;Ko, S.J.;Kim, D.I.;Choi, D.S.;Kim, J.D.;Choi, K.J.
    • Korean Journal of Organic Agriculture
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    • v.19 no.spc
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    • pp.291-294
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    • 2011
  • We studies a model for management of pepper anthracnose based covering method and spraying system in field. 1. Among 82 organic fungicides, 42 materials showed most effective inhibition against mycelia growth of the Colletotrichum acutatum in vitro. 23 formulated biocontrol agents were chosen to control the disease from 42 biocontrol agents in greenhouse. In the end, five kinds (2 plant extracts, 2 biopesiticides, 1 Bordeaux mixture) were selected from 23 materials in the field. 2. The mulching materials of bed covering in fruit season were thin non-woven fabric sheet and black plastic. The use of a fabric sheet was reduced the spread of anthracnose as compared to the plastic covering. 3. The application with the chosen materials was reduced 34% of anthracnose for 7 times sprays to planting 70 days as compared to the untreated control. In yield, nonwoven fabric sheet with formulated biopesticides was increased 17% than black plastic. 4. This result indicated that the developed biocontrol strategy could be an effective and economic crop protection system in organic pepper cultivation field.

A Study on an Operation Mode of a Rotary-type Small-scaled LIM for Railway Transit (철도차량용 회전형 축소모델 선형유도전동기의 운전모드 연구)

  • Park, Chan-Bae;Lee, Byung-Song;Lee, Hyung-Woo;Kwon, Sam-Young;Park, Hyun-June;Han, Kyung-Hee
    • Proceedings of the KSR Conference
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    • 2008.11b
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    • pp.310-315
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    • 2008
  • Authors design a rotary-type small-scaled linear induction motor(LIM) and a performance test machine before manufacturing a real-scaled LIM for a railway transit. The designed LIM is a single-sided, short primary type and its primary has 4 poles. The rated power is 10(kW). In order to analyze characteristics of the LIM, authors use a mixed 2D-3D FEM analysis. 3-D FEM analysis is used for calculating a distribution of eddy-current on the semi-caped AL-sheet of the secondary reaction. Authors calculate a correction factor of conductivity and an equivalent conductivity on the secondary AL-sheet using a normalized eddy-current. The equivalent conductivity which is calculated in this way includes a transverse edge-effect of the LIM. Authors apply the equivalent conductivity on the secondary AL-sheet of 2D-FEM model and get performance characteristics of the LIM. Basic characteristics such as thrust and normal force, input current, efficiency and power factor of the LIM have been analyzed with the variation of frequency and speed. In order to apply an air-gap control system, the variation of the basic characteristics have been analyzed with the air-gap length variation of the LIM. Finally, authors introduce an operation mode using the air-gap control system and conduct a research on feasibility of the system.

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Micro-CT image-based reconstruction algorithm for multiscale modeling of Sheet Molding Compound (SMC) composites with experimental validation

  • Lim, Hyoung Jun;Choi, Hoil;Yoon, Sang-Jae;Lim, Sang Won;Choi, Chi-Hoon;Yun, Gun Jin
    • Composite Materials and Engineering
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    • v.3 no.3
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    • pp.221-239
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    • 2021
  • This paper presents a multiscale modeling method for sheet molding compound (SMC) composites through a novel bundle packing reconstruction algorithm based on a micro-CT (Computed Tomography) image processing. Due to the complex flow pattern during the compression molding process, the SMC composites show a spatially varying orientation and overlapping of fiber bundles. Therefore, significant inhomogeneity and anisotropy are commonly observed and pose a tremendous challenge to predicting SMC composites' properties. For high-fidelity modeling of the SMC composites, the statistical distributions for the fiber orientation and local volume fraction are characterized from micro-CT images of real SMC composites. After that, a novel bundle packing reconstruction algorithm for a high-fidelity SMC model is proposed by considering the statistical distributions. A method for evaluating specimen level's strength and stiffness is also proposed from a set of high-fidelity SMC models. Finally, the proposed multiscale modeling methodology is experimentally validated through a tensile test.

Structural integrity assessment procedure of PCSG unit block using homogenization method

  • Gyogeun Youn;Wanjae Jang;Youngjae Jeon;Kang-Heon Lee;Gyu Mahn Lee;Jae-Seon Lee;Seongmin Chang
    • Nuclear Engineering and Technology
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    • v.55 no.4
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    • pp.1365-1381
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    • 2023
  • In this paper, a procedure for evaluating the structural integrity of the PCSG (Printed Circuit Steam Generator) unit block is presented with a simplified FE (finite element) analysis technique by applying the homogenization method. The homogenization method converts an inhomogeneous elastic body into a homogeneous elastic body with same mechanical behaviour. This method is effective when the inhomogeneous elastic body has repetitive microstructures, and thus the method was applied to the sheet assembly among the PCSG unit block components. From the method, the homogenized equivalent elastic constants of the sheet assembly were derived. The validity of the determined material properties was verified by comparing the mechanical behaviour with the reference model. Thermo-mechanical analysis was then performed to evaluate the structural integrity of the PCSG unit block, and it was found that the contact region between the steam header and the sheet assembly is a critical point where large bending stress occurs due to the temperature difference.

Analytical Evaluation of High Velocity Impact Resistance of Two-way RC Slab Reinforced with Steel Fiber and FRP Sheet (강섬유 및 FRP Sheet로 보강한 2방향 RC 슬래브의 고속 충격저항성능에 대한 해석적 평가)

  • Lee, Jin Young;Shin, Hyen Oh;Min, Kyeng Hwan;Yoon, Young Soo
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.17 no.3
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    • pp.1-9
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    • 2013
  • This paper presents high-velocity impact analysis of two-way RC slabs, including steel fibers and strengthening with fiber reinforced polymer (FRP) sheets for evaluating impact resistance. The analysis uses the LS-DYNA program, which is advanced in impact analysis. The present analysis was performed similarly to the high-velocity impact tests conducted by VTT, the technical research center of Finland, to verify the analysis results. High-velocity impact loads were applied to $2100{\times}2100{\times}250$ mm size two-way RC slab specimens, using a non-deformable steel projectile of 47.5kg mass and 134.9m/s velocity. In this research, extra impact analysis of material specimens was carried out to verify the material models used to the analysis. The elastic-plastic hydrodynamic model, concrete damage model and orthotropic elastic model were used to simulate the non-linear softening behavior of steel fiber reinforced concrete (SFRC), and material properties of normal concrete and FRP sheets, respectively. It is concluded that the suggested analysis technique has good reliability, and can be effectively applied in evaluating the effectiveness of reinforcing/retrofitting materials and techniques. Also, the Steel fiber and FRP sheet strengthening systems provided outstanding performance under high-velocity impact loads.

The Numerical Study on Breakup and Vaporization Process of GDI Spray under High-Temperature and High-Pressure Conditions (고온.고압의 분위기 조건에서 GDI 분무의 분열 및 증발과정에 대한 수치적 연구)

  • 심영삼;황순철;김덕줄
    • Transactions of the Korean Society of Automotive Engineers
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    • v.12 no.3
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    • pp.44-50
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    • 2004
  • The purpose of this study is to improve the prediction ability of the atomization and vaporization processes of GDI spray under high-pressure and high-temperature conditions. Several models have been introduced and compared. The atomization process was modeled using hybrid breakup model that is composed of Conical Sheet Disintegration (CSD) model and Aerodynamically Progressed TAB(APTAB) model. The vaporization process was modeled using Spalding model, modified Spalding model and Abramzon & Sirignano model. Exciplex fluorescence method was used for comparing the calculated with the experimental results. The experiment and calculation were performed at the ambient pressure of 0.5 MPa and 1.0 MPa and the ambient temperature of 473k. Comparison of caldulated and experimental spray characteristics was carried out and Abramzon & Sirignano model and modified Spalding model had the better prediction ability for vaporization process than Spalding model.

Experimental and analytical investigation of steel beams rehabilitated using GFRP sheets

  • El Damatty, A.A.;Abushagur, M.;Youssef, M.A.
    • Steel and Composite Structures
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    • v.3 no.6
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    • pp.421-438
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    • 2003
  • Aging and deterioration of existing steel structures necessitate the development of simple and efficient rehabilitation techniques. The current study investigates a methodology to enhance the flexural capacity of steel beams by bonding Glass Fibre Reinforced Plastic (GFRP) sheets to their flanges. A heavy duty adhesive, tested in a previous study is used to bond the steel and the GFRP sheet. In addition to its ease of application, the GFRP sheet provides a protective layer that prevents future corrosion of the steel section. The study reports the results of bending tests conducted on a W-shaped steel beam before and after rehabilitation using GFRP sheets. Enhancement in the moment capacity of the beam due to bonding GFRP sheet is determined from the test results. A closed form analytical model that can predict the yield moment as well as the stresses induced in the adhesive and the GFRP sheets of rehabilitated steel beam is developed. A detailed finite element analysis for the tested specimens is also conducted in this paper. The steel web and flanges as well as the GFRP sheets are simulated using three-dimensional brick elements. The shear and peel stiffness of the adhesive are modeled as equivalent linear spring systems. The analytical and experimental results indicate that a significant enhancement in the ultimate capacity of the steel beam is achieved using the proposed technique. The finite element analysis is employed to describe in detail the profile of stresses and strains that develop in the rehabilitated steel beam.

Investigation on the Applicability of Defocus Blur Variations to Depth Calculation Using Target Sheet Images Captured by a DSLR Camera

  • Seo, Suyoung
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.38 no.2
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    • pp.109-121
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    • 2020
  • Depth calculation of objects in a scene from images is one of the most studied processes in the fields of image processing, computer vision, and photogrammetry. Conventionally, depth is calculated using a pair of overlapped images captured at different view points. However, there have been studies to calculate depths from a single image. Theoretically, it is known to be possible to calculate depth using the diameter of CoC (Circle of Confusion) caused by defocus under the assumption of a thin lens model. Thus, this study aims to verify the validity of the thin lens model to calculate depth from edge blur amount which corresponds to the radius of CoC. For this study, a commercially available DSLR (Digital Single Lens Reflex) camera was used to capture a set of target sheets which had different edge contrasts. In order to find out the pattern of the variations of edge blur against varying combination of FD (Focusing Distance) and OD (Object Distance), the camera was set to varying FD and target sheet images were captured at varying OD under each FD. Then, the edge blur and edge displacement were estimated from edge slope profiles using a brute-force method. The experimental results show that the pattern of the variations of edge blur observed in the target images was apart from their corresponding theoretical amounts derived under the thin lens assumption but can still be utilized to calculate depth from a single image for the cases similar to the limited conditions experimented under which the tendency between FD and OD is manifest.