• Journal of Embryo Transfer
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• v.23 no.2
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• pp.119-125
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• 2008

A 3-month-old American Cocker spaniel was presented at the Veterinary Teaching Hospital, Chungbuk National University, for examination of urinary tract after dissection of vaginal mass at local clinic before 10 days. Clinical examination of the affected bitch revealed a normal sized vulva in a normal anatomical position with a grossly enlarged clitoris, which contained an os clitoris. On examinations of the genital gland, there were testis, epididymis, ductus deferens and uterus. The histology of both gonads was primarily testis. Seminiferous tubules were divided into many parts by fibrous connective tissue. A small number of spermatogonia was present, but large numbers of Leydig's cells were existed. A normal female karyotype (78, XX) was detected in metaphase spreads obtained from cultured peripheral lymphocytes. Y chromosome specific sequences were not detected in genomic DNA by PCR. After 27 months, the os clitoris was larger than 3-month-old dog and os bone was more calcified than young age. Combining the results of cytogenetic, molecular genetic and histological examinations, the dog was diagnosed as a female hermaphrodite with Sry-negative XX sex reversal.

• Journal of Veterinary Clinics
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• v.29 no.5
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• pp.422-426
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• 2012

A 3-year-old Cocker spaniel was presented for evaluation because of a mass protruding from the vulva. This mass confirmed the presence of an internal bone in clitoris on the radiography. We recommended to the owner of the surgical removal of the gonads and uterus as well as the excision of the enlarged clitoris and os clitoris. The dog had gonads in the region of where the ovaries were located and presented what appeared to be a bicornate uterus and ductus deferens. Histological analysis revealed a morphologically normal uterus and two testes with their epididymis and ductus deferens. Karyotyping revealed a normal female chromosome constitution (78, XX), and polymerase chain reaction (PCR) identified the absence of Sry. Based on these findings, the patient was diagnosed with Sry-negative XX sex reversal.

• Journal of Aquaculture
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• v.9 no.2
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• pp.141-149
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• 1996

Effects of $17{\alpha}$-methyltestosterone ($17{\alpha}-MT$) in rearing water on sex reversal of Nile tilapia (Oreochromis niloticus) were investigated and optimum conditions as initial treatment time, concentrations and treatment durations of hormone were evaluated in this study. For the study of the optimum initial treatment time, 0 to 20-day-old fry were treated by immersion in a 500 ppb solution of $17{\alpha}-MT$. Desirable results were obtained at 12-day-old fish as $86.4\%$ of male induction rates and this frequency was obviously higher than the other age groups. Twelve-day-old fry were immersed in $17{\alpha}-MT$ solutions at concentrations of $0\~2,000$ ppb for 24 hours for the study of dose dependent relationship between sex ratios and concentrations of $17{\alpha}-MT$, Male induction rates of all treated groups were higher than $80\%$, $X^2$ values against the sex ratio of control group indicate that sex reversal has taken place effectively in all experimental groups (P<0.001). Futhermore, 500 ppb treated groups showed highest male incidence of $86.4\%$. Effects of 5 different durations (0, 12, 18, 24 and 30 hours) treated with 1,000 ppb of $17{\alpha}-MT$ were also evaluated. Male induction rate from 0 to 24 hours treated group was increased with treatment durations. However, male incidence of 30-hour treated group ($84.0\%$) was sligthly decreased compare to the 24-hour treated group ($85.2\%$). It is concluded that immersion treatment using $17{\alpha}-MT$ on O. niloticus is possible on sex reversal at low concentration of 500 ppb and short treatment durations within 24 hours with 12-old-day fry.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.6
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• pp.585-590
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• 2009

The purpose of this study was to examine the effects of oral-administered sex hormone for hybrid sturgeon, bester juvenile. The bester juveniles (2 months after hatching) were received a diet containing various doses of $17\alpha$-methyltestosterone (MT) or estradiol-$17\beta$ ($E_2$) for 6 months. Somatic growth of bester sturgeon juvenile did not show significant differences between experimental and control groups (27.9-30.5 cm; 125.1-161.7 g), although survival percentages showed a decreasing tendency in MT-treated animals. By histological examination, germ cells were recorded as smooth type in MT-treated fish and uneven type of germinal epithelium in $E_2$-treated animals. Their sex ratios were 5:4:1 (male: female: undifferentiation) in control and low dose of MT-treated fish (1 mg/kg), and 9:1:0 in fish treated with high dose of MT (10 mg/kg), whereas the ratios were reversed by both low and high doses of $E_2$ treatment, recorded as 2:8:0. Gonadal areas were not significantly differed in all trials (424,600.4 - 1,039,656.3 ${\mu}m^2$). Total number of germ cells, number of germ cells per gonadal areas and number of germ cells per area were significantly higher to 144.7-148.7 cells/section, 374.0-408.5 $cells/mm^2$ and 1,599.5-1,670.9 $cells/mm^2$ in $E_2$ treatment than those of others (30.4-63.9 cells/section, 148.4-226.9 $cells/mm^2$ and 850.0-1,050.6 $cells/mm^2$), respectively. And somatic growth according to their gender was not significantly differed between male and female.

• Journal of Aquaculture
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• v.8 no.2
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• pp.77-83
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• 1995

The effects of six levels (0, 5, 10, 15, 20, and 25 ppm) of dietary $17\alpha-Methyltestosterone$ (MT) on sex reversal and growth of Oreochromis aureus (Steindachner) and O. niloticus (L.) were evaluated. Fifteen ppm MT produced $100\%$ male populations in both species. Both MT-treated and untreated O. niloticus grew significantly faster than their O. aureus counterparts (P<0.05).

• Journal of Aquaculture
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• v.9 no.1
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• pp.101-106
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• 1996

Gynogenetic males induced from sex reversed female (${\Delta}XY$) were crossed with normal female (XX) for analysing their genotypes. The fish tested produced a high percentage of male progenies (93.3 to $100\%$) and were considered as supermales (YY). Superfemales (${\Delta}YY$) were also produced by combination of sex reversal and chromosome manipulation techniques. Superfemale fish can be produced approximetly $90\%$ of male when the fish were crossed with normal male. Chi-square values against an expected 1 : 1 (male : female) ratio were highly significant for both YY males${\times}$ normal females (P<0.01 or P<0.001) and ${\Delta}YY$ females${\times}$normal males (P<0.005 or P<0.001). All male progenies were produced consistently when crossed supermales (YY) with superfemales (${\Delta}YY$).

• Korean Journal of Animal Reproduction
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• v.13 no.1
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• pp.40-48
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• 1989

Guppy fry were treated for the first 40 days of life with 0, 20, 40, 60 & 100$\mu\textrm{g}$ of estradiol per gram of food in order to change the sex of normal males to functional females(genetic male). The present investigation deals with the effects of steroid hormones, such as $\beta$-estradiol and testosterone, on the sex differentiation in guppy and tilapia. The results obtained were summarized as follows. 1. In B (20$\mu\textrm{g}$/g diet) group 17$\beta$-estradiol-treated, 67.8% of male offsprings were produced. 2. In D (60$\mu\textrm{g}$/g diet) group 17$\beta$-treated, 67% of female offsprings were produced. 3. B, D groups of genetic male brooders had significantly different effects (P<0.01) upon sex ratios of their progeny. 4. This strongly indicates that sex direction has been achieved and that the male is the heterogametic sex. 5. The group that produced the highest percentage of male offspring(male percentage of observed number to expected number was 91%) contained only full-sibling male brooders to the sex-reversed female brooders. 6. After 7 months following treatment, the sex-reversed males had ovarian portion in the anterior region and a testicular portion in the posterior region of the same intersexual gonad, respectively. 7. At 7 months after treatment, the ovareis revealed a complete arrest of the ovarian formation, and appearances of spermatogenetic cell cysts among surviving auxocytes. 8. In most of sex-reversed fish, anterior portion of test is was devoid of sperm ducts including the seminal vesicle and vas deferens. 9. The male transferrin showed two strong bands, while the female transferrin showed a single weak band. 10. One of the two bands of male transferrin showed the same mobility with band of female transferrin.

• Journal of Aquaculture
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• v.13 no.4
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• pp.331-338
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• 2000

To establish an alternate technology for all male seed production of Nile tilapia, Oreochromis niloticus at hatchery scale, the performance of progenies produced by crossing superfemale with sex reversed male was evaluated. Free crossings between 21 superfemales and 7 sex reversed males stocked in an aquarium for 60 days resulted in the production of 93.5 % male progenies, compared to 53.2 % males produced by an equal number of normal females and normal males. Spawning frequency was lower (19 times) in the experimental cross including superfemales than that (24 times) in the control including normal females. Seed production in the experimental aquarium was (3,085) significantly lower than that (3,797) of the control. Both in terms of seeds per female per day or seeds per g female per day, seed productivity did not significantly differ between these groups. The alternate technology involving crossing between superfemale and sex reversed male has proven to be an efficient alternate technology for mass production of all male seeds at the hatchery scale.

• Journal of Aquaculture
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• v.18 no.3
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• pp.167-172
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• 2005

Sex reversal to a functional male of sevenband grouper $(41.0{\pm}1.3cm\;TL,\;1.4{\pm}0.1kg\;BW)$ was induced by $17{\alpha}-methyltestosterone\;(MT,\;0.5\sim2.0mg/kg\;BW)$ implantation from March 17 to May 12,2002. Gonad of control group was composed of genial cells and peri-nucleolus oocyte during the experimental period. Gonad of fish treated with 0.5 mg MT/kg BW had peri-nucleolus oocytes, spermatogonia, spermatids and spermatozoa at the late stages of spermatogenesis, while the fish group treated with 1.0 and 2.0 mg MT/kg BW contained spermatoza in the efferent duct. Sperm were obtained from the experimental groups treated with a dose of $1.0{\sim}2.0mg$ MT/kg BW. In the MT treated groups, testosterone and 11-ketotestosterone levels were higher than those in the control group during the $2{\sim}6$ weeks of the experimental period (P<0.05). $Estradiol-17{\beta}$ was detected from fish in the experimental fish.

• Journal of Genetic Medicine
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• v.2 no.1
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• pp.11-15
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• 1998

This paper reports 3 cases with 46,XX sex reversed male. Three 46,XX hypogonadal subjects showed complete sex reversal and had normal phallus and azoospermia. We studied them under clinical, cytogenetic and molecular aspects to find out the origin of the sex reversal. Patients had markedly elevated serum follicle-stimulating hormone (FSH) and lutenizing hormone (LH) and decreased or normal range of serum testosterone. The testicular volumes were small (3-8ml). Testicular biopsy showed Leydig cell hyperplasia and atrophy of seminiferous tubules. We obtained the results of normal 46,XX, and the presence of Y chromosome mosaicism was ruled out through XY dual fluorescent in situ hybridization (FISH). By using polymerase chain reaction (PCR), we amplified short arm (SRY, PABY, ZFY and DYS14), centromere (DYZ3), and heterochromatin (DYZ1) region of the Y chromosome. PCR amplification of DNA from these patients showed the presence of the sex-determining region of the Y chromosome (SRY) but didn't show the centromere and heterochromatin region sequence. The SRY gene was detected in all the three patients. Amplification patterns of the other regions were different in these patients; one had four amplified loci (PABY+, SRY+, ZFY+, DYS14+), another had two loci (SRY+, ZFY+) and the other had two loci (PABY+, SRY+). We have found that each patient's translocation elements had different breakpoints at upstream and downstream of the SRY gene region. We conclude that the testicular development in 46,XX male patients were due to insertion or translocation of SRY gene into X chromosome or autosomes.