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http://dx.doi.org/10.5657/kfas.2009.42.6.585

Effects of Oral-Administered 17α-Methyltestosterone and Estradiol-17β for Sex Reversal of Hybrid Sturgeon, Bester Juvenile  

Kwon, O-Nam (Marine Biology Center for Research & Education, Gangneung-Wonju National University)
Adachi, Shinji (Division of Marine Biosciences, Graduate School of Fisheries Science, Hokkaido University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.42, no.6, 2009 , pp. 585-590 More about this Journal
Abstract
The purpose of this study was to examine the effects of oral-administered sex hormone for hybrid sturgeon, bester juvenile. The bester juveniles (2 months after hatching) were received a diet containing various doses of $17\alpha$-methyltestosterone (MT) or estradiol-$17\beta$ ($E_2$) for 6 months. Somatic growth of bester sturgeon juvenile did not show significant differences between experimental and control groups (27.9-30.5 cm; 125.1-161.7 g), although survival percentages showed a decreasing tendency in MT-treated animals. By histological examination, germ cells were recorded as smooth type in MT-treated fish and uneven type of germinal epithelium in $E_2$-treated animals. Their sex ratios were 5:4:1 (male: female: undifferentiation) in control and low dose of MT-treated fish (1 mg/kg), and 9:1:0 in fish treated with high dose of MT (10 mg/kg), whereas the ratios were reversed by both low and high doses of $E_2$ treatment, recorded as 2:8:0. Gonadal areas were not significantly differed in all trials (424,600.4 - 1,039,656.3 ${\mu}m^2$). Total number of germ cells, number of germ cells per gonadal areas and number of germ cells per area were significantly higher to 144.7-148.7 cells/section, 374.0-408.5 $cells/mm^2$ and 1,599.5-1,670.9 $cells/mm^2$ in $E_2$ treatment than those of others (30.4-63.9 cells/section, 148.4-226.9 $cells/mm^2$ and 850.0-1,050.6 $cells/mm^2$), respectively. And somatic growth according to their gender was not significantly differed between male and female.
Keywords
Hybrid sturgeon; Gonad; Germ cell; Sex reversal; Hormone treatment; Bester;
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