• Title/Summary/Keyword: Sesamum indicum L.

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Macrophomina phaseolina Detected in Seeds of Sesamum indicum and It's Pathogenicity (참깨 종자에서 검출된 Macrophomina phaseolina와 그의 병원성에 관하여)

  • YU S.H.;Park J.S.
    • Korean journal of applied entomology
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    • v.19 no.3 s.44
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    • pp.135-140
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    • 1980
  • Out of 12 seed samples of Sesamum indicum L. tested, Macrophomina phaseolina (Tassi.) Goid was detected in 7 samples for the first time in Korea. Detailed descriptions of the habit character and pycnidial and pycnospore morphology of this fungus were described. Pycnidia of this fungus were not formed on agar media but they were formed on Water Agar Leaf Media under fluorescent light. M. phaseolina caused heavy reduction in seed germination and seedling stand of sesame and produced charcoal rot symptom on potato tubers. It was also detected from over wintered plant debris and diseased seedlings in the field.

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Anti-oxidant Function and Inhibitory Effects of the Expression of MITF, TRP-1, TRP-2 and Tyrosinase of Sesamum indicum L. in B16F10 Melanoma Cells (참깨 추출물의 항산화 활성 및 melanoma cell (B16F10)에서 MITF, TRP-1, TRP-2, tyrosinase 의 발현 저해)

  • Yoo, Dan-Hee;Joo, Da-Hye;Lee, Jin-Young
    • Journal of Life Science
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    • v.27 no.3
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    • pp.318-324
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    • 2017
  • This study was performed to improve the antioxidant and skin-whitening activities of 70% ethanol extract from Sesamum indicum L. (SIL). The electron-donating ability of the SIL extract was 71.7% at a concentration of $1,000{\mu}g/ml$. The whitening effects that was measured by tyrosinase inhibition assay. As a result, SIL extract was shown 42% at $1,000{\mu}g/ml$ concentration. The cell toxicity on B16F10 melanoma cells of SIL of 70% ethanol extract showed 84.3% at $1,000{\mu}g/ml$ concentration. The microphthalmia-associated transcription factor (MITF), tyrosinase relate protein-1 (TRP-1), tyrosinase relate protein-2 (TRP-2) and Tyrosinase protein and mRNA expression inhibitory effect of SIL extract were measured by western blot and reverse transcription- polymerase chain reaction (PCR) at 50, 250, $500{\mu}g/ml$ concentration. Consequently, the MITF, TRP-1, TRP-2, Tyrosinase protein expression inhibitory effect of SIL extract was decreased by 68.3%, 39.2%, 89.7%, 22.3%, respectively, at $500{\mu}g/ml$ concentration. Moreover, MITF, TRP-1, TRP-2, Tyrosinase mRNA expression inhibitory effect by reverse-transcription-PCR of SIL extract was decreased by 81.8%, 66.5%, 84.2%, 68.1%, respectively, at $500{\mu}g/ml$ concentration. Therefore, we excellently identified the antioxidant activities and whitening effect of SIL extract, and this finding suggested that SIL extract has great potential as a cosmetic ingredients.