• Korean Journal of Veterinary Research
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• v.38 no.4
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• pp.859-866
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• 1998

This study was carried out to investigate the prevalence of Neopsora (N) caninum infection in Korean dairy herds. To determine the prevalence of antibodies to N caninum in Korean dairy cattle, a total of 1,688 sera including 895 sera taken from 30 herds having recent high abortion rate and 793 sera selected randomly from 168 herds with no history of recent abortion problem, respectively, collected nationwide during a designated period were analyzed by indirect fluorescent antibody (IFA) test. Mean nationwide seropositive rate of the sera tested in herds and individual cattle tested were 53.5% and 35.6%, respectively. However mean seropositive rate of the samples from herds having abortion problem was approximately two and half times higher than those in herds with no recent abortion history. Regional seropositive rates of the samples from the herds with abortion problem were 48.6%, 51.6%, 44.4% and 71.4% at Kyunggi, Kangwon, Kyungbuk and Jeonnam province, respetively. Regional seropositive rates of the samples from the herds with no recent abortion problem were 35.6%, 18.3%, 16.5%, 37.5%, 19.4%, 33.3%, 32.1%, 3.8% and 0.0% at Kyunggi, Kangwon, Chungbuk, Chungnam, Kyungbuk, Kyungnam, Jeonbuk, Jeonnam and Jeju province, respectively. The results of this study suggested that N caninum infection was widespread and considerably associated with bovine abortion in Korea.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.19 no.2
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• pp.110-115
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• 2016

Purpose: We performed to reveal the association between the Helicobacter pylori infection and body weight among children. Methods: Out retrospective study included patients who underwent the H. pylori immunoglobulin G testing at Konyang University Hospital between March 2011 and June 2014. These patients were classified as seropositive (28 boys, 27 girls; mean age: $9.89{\pm}3.28years$) or seronegative (55 boys, 54 girls; mean age: $9.84{\pm}3.02years$). Next, we compared various characteristics between the seropositive and negative groups, as well as between obese children (body weight ${\geq}90th$ percentile) and non-obese children (body weight <90th percentile). Furthermore, we compared the change in body weight after 2 months of treatment with amoxicillin, clarithromycin and omeprazole among the 55 seropositive children (14 treated children and 41 non-treated children). Results: There were no differences in the weights and laboratory data for the 55 seropositive children and 109 seronegative children (weight; $40.96{\pm}18.11kg$ vs. $36.85{\pm}13.72kg$, respectively; p=0.14). And, there was no difference in the prevalence of H. pylori infection among the 29 obese and 135 non-obese children (p=0.581). However, after 2 months of eradication, the 14 treated patients exhibited a significant weight gain ($+0.91{\pm}0.52kg$), compared to the 41 non-treated patients ($-0.29{\pm}1.16kg$, p=0.025). Conclusion: Our findings present that obesity was not associated with the H. pylori infection, although H. pylori eradication led to significant increase in body weight.

• Parasites, Hosts and Diseases
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• v.47 no.2
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• pp.125-130
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• 2009

To figure out the epidemiological status and relevance with other diseases in toxoplasmosis, we checked serum IgG antibody titers of 1,265 patients and medical records of seropositive patients. Seropositive rates were 6.6% by latex agglutination test (LAT) and 6.7% by ELISA. No significant differences were detected between sexes and age groups. The peak seroprevalence was detected in the 40-49-year-old age group. According to clinical department, Toxoplasma-positive rates were high in patients in psychiatry, ophthalmology, health management, emergency medicine, and thoracic surgery. Major coincidental diseases in seropositive cases were malignant neoplasms, diabetes mellitus, arthritis, chronic hepatitis B, chronic renal diseases, schizophrenia, and acute lymphadenitis, in the order of frequency. In particular, some patients with chronic hepatitis B and malignant neoplasms had high antibody titers. These results revealed that the seroprevalence of toxoplasmosis in a general hospital-based study was similar to that in a community-based study, and T. gondii seropositivity may be associated with neoplasms, diabetes, and other chronic infections.

• Korean Journal of Veterinary Service
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• v.34 no.3
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• pp.245-250
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• 2011

In the present study, we carried out a seropositive survey of Neospora (N) caninum and Toxoplasma (T) gondii in cattle at northern area of Gyeongnam province, Korea. Serum sample was obtained from Holstein cattle (n=248, 44 farms in 5 counties) and Korean indigenous cattle (n=120, 23 farms in 4 counties). All 368 sera were tested for antibodies to N. caninum and T. gondii by an enzyme-linked immunosorbent assay (ELISA). Seropositive of N. caninum at farm and heads were 41.8% (28/67) and 12.8% (47/368), respectively. Twenty-five cattle (6.8%) were seropositive to T. gondii, and eighteen (26.9%) of 67 studied farms had cows seropositive to T. gondii. The seroprevalence of Holstein cattle was much higher than that of Korean indigenous cattle. There was no apparent association of T. gondii seropositivity with age, while the seropositivity for N. caninum was associated with cattle age. The farm of cattle above the age of 5 years (16.8%) was more infective than less than 4 years (9.3%). This is the first report on seroprevalence of N. canium and T. gondii in Holstein and Korean indigenous cattle in Gyeongnam, Korea.

• Korean Journal of Veterinary Research
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• v.30 no.3
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• pp.343-348
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• 1990

Total 51 calves born from both 28 seropositive and 23 seronegative dams were subjected to study both prenatal and postnatal infections of bovine leukemia virus (BLV), and the duration of passive colostral antibody by means of immunodiffusion (ID) test. All calves were tested for precolostral and postcolostral periods by 16 months of age. The results were as follows: 1. Of 28 precolostral sera of the calves born from infected dams, one appeared positive, indicating in utero BLV infection from the dam. 2. BLV-antibody test for the postcolostral sera of the calves born from seropositive or seronegative dams showed that the colostral antibody of the calves disappeared from 2 to 6 months of age, and the increase of the number of seropositive calves initiated from 3 to 4 months of age indicated postnatal infection.

• Korean Journal of Veterinary Research
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• v.47 no.3
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• pp.281-283
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• 2007

This study was conducted to survey of Ehrlichia (E.) canis and Borrelia (B.) burgdorferi antibodies among clinically healthy German shepherd dogs (116 females and 120 males) using a ELISA kit (SNAP 3Dx; IDEXX Laboratories, USA) in Korea. Whole blood samples are collected from the 236 dogs and are tested to detect E. canis and B. burgdorferi antibodies by using ELISA kit (SNAP 3Dx; IDEXX Laboratories, USA). Confidence interval comparisions revealed that dogs of 4-6 years have higher prevalence with a seropositive result (CI=0.17-0.45) in E. canis than the other age groups but there are no differences among age groups in B. burgdorferi. Also, no differences with a seropositive result were found among different regions in E. canis and B. burgdorferi antibodies. In conclusion, this study was the first large scale survey of canine E. canis and B. burgdorferi antibodies in Korea and provide an useful reference for clinicians.

• Korean Journal of Veterinary Research
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• v.58 no.3
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• pp.147-151
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• 2018

We assessed the seroprevalence of Coxiella burnetii (C. burnetii) in cattle on Ulleung Island, Korea in a population-based follow up study for 4 years and determined the spatial distribution and risk factors associated with C. burnetii. The seroprevalence of C. burnetii was determined to be 1.4-2.0% during 2011-2014. Overall, nine cattle from three farms that tested seropositive showed C. burnetii antibody seroconversions between 2011 and 2014. The number of seropositive cattle was low, suggesting that movement of and contact between animals was possible risk factors for the transmission of C. burnetii.

• Parasites, Hosts and Diseases
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• v.47 no.1
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• pp.65-68
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• 2009

Excretory-secretory products of Toxocara canis larvae have been considered as a major functional antigen in immune responses against toxocariasis. We studied ultrastructural localization of T. canis second-stage larval antigen using a seropositive human serum under immunogold electron microscopy. High-density gold particles were observed in the secretory cells, excretory duct, intestinal epithelium, and cuticle of the larval worm sections. The distribution of the positive reactions in the larval worms suggests that the nature of the antigen is excretory-secretory antigen including waste metabolites and secretory enzymes.

• Journal of Veterinary Clinics
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• v.20 no.3
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• pp.427-430
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• 2003

Akabane virus is a cause of severe congenital defects, but adult animals show no signs of infection. In this study, congenital abnormalitis associated with Akabane virus infection in Korean native goat. The prevalence of serum neutralizing antibodies to Akabane virus in goat population was investigated, indicating that approximately 30% of goats in Korea were seropositive(36/120). The mother goats have the highest titers of neutralizing antibodies, as 1:128. And also there showed seropositive of Akabane virus in newborn fetus fluids. The necropsy results of newborn fetus visceral organs were appeared normal. These findings provide that Akabane virus is the ethiological agent of congenital abnormalitis and stillbirth. Our results suggest that goat in natural situations are part of the Akabane virus transmission cycle.

• Korean Journal of Veterinary Research
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• v.55 no.3
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• pp.185-189
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• 2015

Bovine parainfluenza virus type 5 (bPIV5) was isolated from cattle with downer cow syndrome in 2012, and included both respiratory and neurotropic pathogens from a variety of animals. In the current study, we conducted serosurveillance using sera obtained from seven Korean farms and optimized a reverse transcription-polymerase chain reaction (RT-PCR) assay to detect bPIV5. The overall seropositive rate for Korean cattle was 21.4% (163/760). A farm located near the city of Milyang in Gyeoungnam province had a markedly elevated seropositive rate for bPIV5 compared to that of the other six farms. The regional seropositive rates were 4.2% (8/192) for Haman, 19.5% (18/55) for Hwasung, 73.9% (65/88) for Milyang, 26.0% (50/192) for Namwon, 1.0% (1/96) for Uljin, 13.5% (13/96) for Yeongju, and 32.7% (8/41) for Yongin. The sensitivity and specificity of three RT-PCR primer sets used to amplify the conserved fusion gene of bPIV5 were also evaluated. An RT-PCR assay using the bPIVFR3 primer set was 10-fold more sensitive than the assays using the two other primer sets and did not result in non-specific amplification. These results demonstrated that the bPIFR3 primer set can be used to detect bPIV5.